LOCUS       sequence-197764-       10670 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast two hybrid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10670)
  AUTHORS   Gao W, Yang J, Liu W, Wang Y, Shao F
  TITLE     Site-specific phosphorylation and microtubule dynamics control Pyrin
            inflammasome activation.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Aug 16;113(33):E4857-66. doi: 
            10.1073/pnas.1601700113. Epub 2016 Aug 1.
  PUBMED    27482109
REFERENCE   2  (bases 1 to 10670)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1601700113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-08-16- 16"; volume: "113"; issue: "33"; pages: 
            "E4857-66"
COMMENT     Sequence Label: pGAD-GH-Mefv iso1
FEATURES             Location/Qualifiers
     source          1..10670
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..705
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             751..771
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             787..1128
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 activation domain
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     polyA_signal    3641..3775
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3665..3684)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3719..3738
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(3884..3902)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3909..3926)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3909..3925)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3918..3940)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      4328..4515
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(4383..4407)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(4632..5726)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(5727..6132)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     primer_bind     complement(6174..6190)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6174..6190)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6187..6209)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6198..6214
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6222..6228,6229..6246,6247..6252))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6267..6288
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     protein_bind    6343..6376
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    6454..6487
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(6556..6573)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6727..7315)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6807..6826)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7486..8277,8278..8346))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8109..8128
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8347..8451)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8519..8537
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8575..8597)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8697..8716
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      8733..9897
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
ORIGIN
        1 atccttttgt tgtttccggg tgtacaatat ggacttcctc ttttctggca accaaaccca
       61 tacatcggga ttcctataat accttcgttg gtctccctaa catgtaggtg gcggagggga
      121 gatatacaat agaacagata ccagacaaga cataatgggc taaacaagac tacaccaatt
      181 acactgcctc attgatggtg gtacataacg aactaatact gtagccctag acttgatagc
      241 catcatcata tcgaagtttc actacccttt ttccatttgc catctattga agtaataata
      301 ggcgcatgca acttcttttc tttttttttc ttttctctct cccccgttgt tgtctcacca
      361 tatccgcaat gacaaaaaaa tgatggaaga cactaaagga aaaaattaac gacaaagaca
      421 gcaccaacag atgtcgttgt tccagagctg atgaggggta tctcgaagca cacgaaactt
      481 tttccttcct tcattcacgc acactactct ctaatgagca acggtatacg gccttccttc
      541 cagttacttg aatttgaaat aaaaaaaagt ttgctgtctt gctatcaagt ataaatagac
      601 ctgcaattat taatcttttg tttcctcgtc attgttctcg ttccctttct tccttgtttc
      661 tttttctgca caatatttca agctatacca agcatacaat caactccaag ctttgcaaag
      721 atggataaag cggaattaat tcccgagcct ccaaaaaaga agagaaaggt cgaattgggt
      781 accgccgcca attttaatca aagtgggaat attgctgata gctcattgtc cttcactttc
      841 actaacagta gcaacggtcc gaacctcata acaactcaaa caaattctca agcgctttca
      901 caaccaattg cctcctctaa cgttcatgat aacttcatga ataatgaaat cacggctagt
      961 aaaattgatg atggtaataa ttcaaaacca ctgtcacctg gttggacgga ccaaactgcg
     1021 tataacgcgt ttggaatcac tacagggatg tttaatacca ctacaatgga tgatgtatat
     1081 aactatctat tcgatgatga agatacccca ccaaacccaa aaaaagagat cctagaacta
     1141 gtggatcccc cgggctgcag gaattcaggc cggccaatgg ccaagaccct tggtgatcat
     1201 ctgctaaaca ccctggagga gctgctgccc tatgactttg agaagttcaa gtttaagcta
     1261 cagaacacca gcctggagaa gggccactct aagatccccc ggggccatat gcagatggcc
     1321 aggccagtaa agctggccag ccttctgata acctactacg gggaggagta tgctgtaagg
     1381 ctgaccctgc agatcctgcg ggccaccaac cagcgccaac tagcagagga gcttcgcaaa
     1441 gccacaggca cagaacattt aattgaagaa aatagagttg gtggttctgt ccagtcttct
     1501 gtagagaata aagccaagag tgtgaaggta ccagatgtcc ctgaaggtga tgggacacag
     1561 caaaacaatg atgaatcaga cacccttcca tccagccagg ctgaagtggg gaagggaccc
     1621 cagaagaagt cactgaccaa aaggaaggat cagaggggcc ccgagagcct ggactcacag
     1681 accaagccat ggacaaggag cacagcgcct ctctatagga gaacccaggg cacccagtcc
     1741 ccaggggata aggagagcac agcaagtgcc cagctccgca ggaatgtcag ctctgcaggg
     1801 aggcttcaag gactttacaa caatgcccca gggaggagag aaagcaagaa agccgaagtg
     1861 tatgtatatt tgccttcagg aaagaagcga cccaggagtc ttgaaattac cacttattca
     1921 agagaaggag aacccccaaa ttcagaagtt cttccaactc aagaggaaac aagaaatggg
     1981 agtctcattc gcatgagaac agctactctg aatggaagga ctacgggggc tttagaaaag
     2041 ggcacaggga ttccagagca ttccatggtg ctggatgaaa aaacattcag aaacatgtct
     2101 tccaaaacat cgttgattgg ggaggagaga tgccctacat catggacgga aaatggaaat
     2161 gggagtccag aaaccacaga gtcctcggga gagacggctg gcagcatact ctctgatcca
     2221 gaagtgcctt tgtcactatg tgaaaaacca gctaaaactc cagaagaccc agcatcctta
     2281 ggacaggccg cttgtgaagg taccctcctt caggtctcaa gaaaatgtga tgcaggccca
     2341 gaaaaatgca catttacaca tcttgcacta caggttgcat caaatttcgg aaggtcacag
     2401 gacaaggctg tgtgccctct ttgccacacc caggaaggag acctgcgtgg tgatacctgt
     2461 gtgcaaagtt cctgtagctg ctccattgct cctggggatc ccaaggcctc cggcagatgc
     2521 tccatatgct ttcagtgcca aggcttactt gccagaaaga gctgcgaagc ccagagcccc
     2581 cagtccctac cacagtgccc tcgtcacatg aagcaggtgc tgctgctttt ctgtgaggac
     2641 cacagggagc ccatctgcct catctgcagg ctgagcctgg agcatcaagg acatcgagtg
     2701 cgccccatag aggaggctgc actagagtac aaggagcaga tccgggagca gctggagcgc
     2761 ctgcgggaga tgaggggata tgtggaggag cacaggctac agggagacaa gaaaacagac
     2821 gatttcctga aacaaacaga aatccagaag cagaagattt catgtccact tgagaagcta
     2881 taccaactcc tggagaagca agaacaactc tttgtgacct ggctacagga gctaagccag
     2941 accatcagca aggtcagaga gacatactac acccgggttt ccctactgga tgagatgatt
     3001 gaagaactag aggccaagca agaccagcca gagtgggacc tcatgcagga cattggaatc
     3061 accttgcaca gggctaagat gatgtctgcc tctgagctgt tggacactcc tccaggtgtt
     3121 aaagaaaagc ttcatctgct ctaccagaag tcaaagtctg tggagaagaa tatgcagtgt
     3181 ttctcagaaa tgctgagctc tgaaatggca ttcagcgctt cagatgtagc aaaatgggaa
     3241 gggcgccagc ctagcgccac acaggtccag ggcttggttc ccaccgttca cctaaagtgt
     3301 gatggtgcac acacacagga ctgtgatgta gtattttacc cagaacggga agctggagga
     3361 tcagaaccta aagattacct tcatcctcag ccagctcaag acacacctga gctacatgag
     3421 atccattctc ggaacaacaa aagaaaattt aaatctttcc tgaaatggaa accttcattc
     3481 attgggagca ccttgggaga agcccacaga tccgcactcc cagctaggca ttcttcttcc
     3541 tgcagttacc gtgatttgga ccaagcagct gctcacccca acctcatctt ctcgatgata
     3601 tgaggcgcgc ctctagaact atagtgagtc gtattacgta gatccagaca tgataagata
     3661 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga
     3721 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
     3781 caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaatt
     3841 cgcggccgcg gcgccaatgc attgggcccg gtacccaatt cgccctatag tgagtcgtat
     3901 tacaattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctga tctatgaatc
     3961 gtagatactg aaaaaccccg caagttcact tcaactgtgc atcgtgcacc atctcaattt
     4021 ctttcattta tacatcgttt tgccttcttt tatgtaacta tactcctcta agtttcaatc
     4081 ttggccatgt aacctctgat ctatagaatt ttttaaatga ctagaattaa tgcccatctt
     4141 ttttttggac ctaaattctt catgaaaata tattacgagg gcttattcag aagctttgga
     4201 cttcttcgcc agaggtttgg tcaagtctcc aatcaaggtt gtcggcttgt ctaccttgcc
     4261 agaaatttac gaaaagatgg aaaagggtca aatcgttggt agatacgttg ttgacacttc
     4321 taaataagcg aatttcttat gatttatgat ttttattatt aaataagtta taaaaaaaat
     4381 aagtgtatac aaattttaaa gtgactctta ggttttaaaa cgaaaattct tattcttgag
     4441 taactctttc ctgtaggtca ggttgctttc tcaggtatag catgaggtcg ctcttattga
     4501 ccacacctct accggccggt cgaaattccc ctaccctatg aacatattcc attttgtaat
     4561 ttcgtgtcgt ttctattatg aatttcattt ataaagttta tgtacaaata tcataaaaaa
     4621 agagaatctt tttaagcaag gattttctta acttcttcgg cgacagcatc accgacttcg
     4681 gtggtactgt tggaaccacc taaatcacca gttctgatac ctgcatccaa aaccttttta
     4741 actgcatctt caatggcctt accttcttca ggcaagttca atgacaattt caacatcatt
     4801 gcagcagaca agatagtggc gatagggttg accttattct ttggcaaatc tggagcagaa
     4861 ccgtggcatg gttcgtacaa accaaatgcg gtgttcttgt ctggcaaaga ggccaaggac
     4921 gcagatggca acaaacccaa ggaacctggg ataacggagg cttcatcgga gatgatatca
     4981 ccaaacatgt tgctggtgat tataatacca tttaggtggg ttgggttctt aactaggatc
     5041 atggcggcag aatcaatcaa ttgatgttga accttcaatg taggaaattc gttcttgatg
     5101 gtttcctcca cagtttttct ccataatctt gaagaggcca aaacattagc tttatccaag
     5161 gaccaaatag gcaatggtgg ctcatgttgt agggccatga aagcggccat tcttgtgatt
     5221 ctttgcactt ctggaacggt gtattgttca ctatcccaag cgacaccatc accatcgtct
     5281 tcctttctct taccaaagta aatacctccc actaattctc tgacaacaac gaagtcagta
     5341 cctttagcaa attgtggctt gattggagat aagtctaaaa gagagtcgga tgcaaagtta
     5401 catggtctta agttggcgta caattgaagt tctttacgga tttttagtaa accttgttca
     5461 ggtctaacac tacctgtacc ccatttagga ccacccacag cacctaacaa aacggcatca
     5521 gccttcttgg aggcttccag cgcctcatct ggaagtggaa cacctgtagc atcgatagca
     5581 gcaccaccaa ttaaatgatt ttcgaaatcg aacttgacat tggaacgaac atcagaaata
     5641 gctttaagaa ccttaatggc ttcggctgtg atttcttgac caacgtggtc acctggcaaa
     5701 acgacgatct tcttaggggc agacattaga atggtatatc cttgaaatat atatatatat
     5761 tgctgaaatg taaaaggtaa gaaaagttag aaagtaagac gattgctaac cacctattgg
     5821 aaaaaacaat aggtccttaa ataatattgt caacttcaag tattgtgatg caagcattta
     5881 gtcatgaacg cttctctatt ctatatgaaa agccggttcc ggcgctctca cctttccttt
     5941 ttctcccaat ttttcagttg aaaaaggtat atgcgtcagg cgacctctga aattaacaaa
     6001 aaatttccag tcatcgaatt tgattctgtg cgatagcgcc cctgtgtgtt ctcgttatgt
     6061 tgaggaaaaa aataatggtt gctaagagat tcgaactctt gcatcttacg atacctgagt
     6121 attcccacag ttgggggatc tcgactctag ctagaggatc aattcgtaat catgtcatag
     6181 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     6241 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     6301 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgataacttc gtataatgta
     6361 tgctatacga agttattagg tctgaagagg agtttacgtc cagccaagct agcttggctg
     6421 caggtcgagc ggccgcgatc cggaaccctt aatataactt cgtataatgt atgctatacg
     6481 aagttatcag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     6541 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     6601 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     6661 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     6721 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     6781 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     6841 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     6901 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     6961 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     7021 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     7081 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     7141 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     7201 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     7261 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     7321 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     7381 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     7441 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     7501 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     7561 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     7621 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     7681 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     7741 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     7801 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     7861 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     7921 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     7981 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     8041 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     8101 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     8161 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     8221 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     8281 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     8341 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     8401 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     8461 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
     8521 taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg
     8581 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
     8641 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc
     8701 atcagagcag attgtactga gagtgcacca taacgcattt aagcataaac acgcactatg
     8761 ccgttcttct catgtatata tatatacagg caacacgcag atataggtgc gacgtgaaca
     8821 gtgagctgta tgtgcgcagc tcgcgttgca ttttcggaag cgctcgtttt cggaaacgct
     8881 ttgaagttcc tattccgaag ttcctattct ctagctagaa agtataggaa cttcagagcg
     8941 cttttgaaaa ccaaaagcgc tctgaagacg cactttcaaa aaaccaaaaa cgcaccggac
     9001 tgtaacgagc tactaaaata ttgcgaatac cgcttccaca aacattgctc aaaagtatct
     9061 ctttgctata tatctctgtg ctatatccct atataaccta cccatccacc tttcgctcct
     9121 tgaacttgca tctaaactcg acctctacat tttttatgtt tatctctagt attactcttt
     9181 agacaaaaaa attgtagtaa gaactattca tagagtgaat cgaaaacaat acgaaaatgt
     9241 aaacatttcc tatacgtagt atatagagac aaaatagaag aaaccgttca taattttctg
     9301 accaatgaag aatcatcaac gctatcactt tctgttcaca aagtatgcgc aatccacatc
     9361 ggtatagaat ataatcgggg atgcctttat cttgaaaaaa tgcacccgca gcttcgctag
     9421 taatcagtaa acgcgggaag tggagtcagg ctttttttat ggaagagaaa atagacacca
     9481 aagtagcctt cttctaacct taacggacct acagtgcaaa aagttatcaa gagactgcat
     9541 tatagagcgc acaaaggaga aaaaaagtaa tctaagatgc tttgttagaa aaatagcgct
     9601 ctcgggatgc atttttgtag aacaaaaaag aagtatagat tctttgttgg taaaatagcg
     9661 ctctcgcgtt gcatttctgt tctgtaaaaa tgcagctcag attctttgtt tgaaaaatta
     9721 gcgctctcgc gttgcatttt tgttttacaa aaatgaagca cagattcttc gttggtaaaa
     9781 tagcgctttc gcgttgcatt tctgttctgt aaaaatgcag ctcagattct ttgtttgaaa
     9841 aattagcgct ctcgcgttgc atttttgttc tacaaaatga agcacagatg cttcgttgct
     9901 tgcatgcctg caggtcgaga tccgggatcg aagaaatgat ggtaaatgaa ataggaaatc
     9961 aaggagcatg aaggcaaaag acaaatataa gggtcgaacg aaaaataaag tgaaaagtgt
    10021 tgatatgatg tatttggctt tgcggcgccg aaaaaacgag tttacgcaat tgcacaatca
    10081 tgctgactct gtggcggacc cgcgctcttg ccggcccggc gataacgctg ggcgtgaggc
    10141 tgtgcccggc ggagtttttt gcgcctgcat tttccaaggt ttaccctgcg ctaaggggcg
    10201 agattggaga agcaataaga atgccggttg gggttgcgat gatgacgacc acgacaactg
    10261 gtgtcattat ttaagttgcc gaaagaacct gagtgcattt gcaacatgag tatactagaa
    10321 gaatgagcca agacttgcga gacgcgagtt tgccggtggt gcgaacaata gagcgaccat
    10381 gaccttgaag gtgagacgcg cataaccgct agagtacttt gaagaggaaa cagcaatagg
    10441 gttgctacca gtataaatag acaggtacat acaacactgg aaatggttgt ctgtttgagt
    10501 acgctttcaa ttcatttggg tgtgcacttt attatgttac aatatggaag ggaactttac
    10561 acttctccta tgcacatata ttaattaaag tccaatgcta gtagagaagg ggggtaacac
    10621 ccctccgcgc tcttttccga tttttttcta aaccgtggaa tatttcggat
//
