LOCUS       sequence-197769-        9557 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of human codon optimized human SNF2L and pRbAp48 from 
            pFastBac dual.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9557)
  TITLE     pFastBac1_FLAG-BPTF
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9557)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pFastBac_Dual_SNF2L-opt_p48
FEATURES             Location/Qualifiers
     source          1..9557
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      102..556
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(189..208)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     398..419
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        582..686
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(687..755,756..1547)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(905..924)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1718..2306
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2207..2226
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2460..2477
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  2611..2835
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2902..3435)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3605..3610,3611..3627,3628..3633))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     polyA_signal    4141..4189
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     4214..4233
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     promoter        complement(7463..7572)
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     promoter        7596..7687
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     7612..7629
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     polyA_signal    9147..9281
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9184..9203)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9238..9257
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  9310..9475
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 ttctctgtca cagaatgaaa atttttctgt catctcttcg ttattaatgt ttgtaattga
       61 ctgaatatca acgcttattt gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
      121 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
      181 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
      241 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
      301 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
      361 ttttcgccct ttgacgttgg agtccacgtt cttaatagtg gactcttgtt ccaaactgga
      421 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
      481 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
      541 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
      601 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
      661 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
      721 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
      781 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
      841 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
      901 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
      961 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     1021 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     1081 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     1141 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     1201 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     1261 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     1321 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     1381 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     1441 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     1501 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     1561 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     1621 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     1681 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     1741 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     1801 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     1861 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     1921 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     1981 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     2041 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     2101 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     2161 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     2221 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     2281 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     2341 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     2401 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     2461 gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc
     2521 tgtgcggtat ttcacaccgc atagaccagc cgcgtaacct ggcaaaatcg gttacggttg
     2581 agtaataaat ggatgccctg cgtaagcggg tgtgggcgga caataaagtc ttaaactgaa
     2641 caaaatagat ctaaactatg acaataaagt cttaaactag acagaatagt tgtaaactga
     2701 aatcagtcca gttatgctgt gaaaaagcat actggacttt tgttatggct aaagcaaact
     2761 cttcattttc tgaagtgcaa attgcccgtc gtattaaaga ggggcgtggc caagggcatg
     2821 gtaaagacta tattcgcggc gttgtgacaa tttaccgaac aactccgcgg ccgggaagcc
     2881 gatctcggct tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa agtgcatcac
     2941 ttcttcccgt atgcccaact ttgtatagag agccactgcg ggatcgtcac cgtaatctgc
     3001 ttgcacgtag atcacataag caccaagcgc gttggcctca tgcttgagga gattgatgag
     3061 cgcggtggca atgccctgcc tccggtgctc gccggagact gcgagatcat agatatagat
     3121 ctcactacgc ggctgctcaa acttgggcag aacgtaagcc gcgagagcgc caacaaccgc
     3181 ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta
     3241 atcggagtcc ggctgatgtt gggagtaggt ggctacgtct ccgaactcac gaccgaaaag
     3301 atcaagagca gcccgcatgg atttgacttg gtcagggccg agcctacatg tgcgaatgat
     3361 gcccatactt gagccaccta actttgtttt agggcgactg ccctgctgcg taacatcgtt
     3421 gctgctccat aacatcaaac atcgacccac ggcgtaacgc gcttgctgct tggatgcccg
     3481 aggcatagac tgtacaaaaa aacagtcata acaagccatg aaaaccgcca ctgcgccgtt
     3541 accaccgctg cgttcggtca aggttctgga ccagttgcgt gagcgcatac gctacttgca
     3601 ttacagttta cgaaccgaac aggcttatgt caactgggtt cgtgccttca tccgtttcca
     3661 cggtgtgcgt cacccggcaa ccttgggcag cagcgaagtc gaggcatttc tgtcctggct
     3721 ggcgaacgag cgcaaggttt cggtctccac gcatcgtcag gcattggcgg ccttgctgtt
     3781 cttctacggc aaggtgctgt gcacggatct gcccttgctt caggagatcg gtagacctcg
     3841 gccgtcgcgg cgcttgccgg tggtgctgac cccggatgaa gtggttcgca tcctcggttt
     3901 tctggaaggc gagcatcgtt tgttcgccca ggactctagc tatagttcta gtggttggct
     3961 acgtacccgt agtggctatg gcagggcttg ccgccccgac gttggctgcg agccctgggc
     4021 cttcacccga acttgggggt tggggtgggg aaaaggaaga aacgcgggcg tattggtccc
     4081 aatggggtct cggtggggta tcgacagagt gccagccctg ggaccgaacc ccgcgtttat
     4141 gaacaaacga cccaacaccc gtgcgtttta ttctgtcttt ttattgccgt catagcgcgg
     4201 gttccttccg gtattgtctc cttccgtgtt tcagttagcc tcccccatct cccggtacct
     4261 tacgacttca ccttcttcac atccttcttg cccgacgatt cagtagccga ctcagccttt
     4321 ctcttttggg acattggagt cttggtggca cgcttcttct tctcagcgcg ttcacgctct
     4381 tcgatctcca tgttctcctt ttcgatcaga gagatcaaag tgttacatct cctctggaac
     4441 tccatggcgg tgcgggactt gatgaaccag tcgaaacgga attgaggagc gttgcgaaca
     4501 cactgacgca gctcttcgta gacgttctca cgatcgaatc ccatcttgtg caacatgcag
     4561 atgaggaacc tgtcctcttc ctctgtgtag ttctttccct ttgaagtgcc gtattggatt
     4621 ctcagctggt ggaagggggc cttgtacctg gcgatcttag cgtccaaggc cttcttgatg
     4681 ctgatgcgac gctggattct ggcctcgccc ctttcgattt gagccatgat cttctcgatg
     4741 tcctgcagct cgttgcaacg ttcccagaac acagccgagt actccataac ttcctcaggt
     4801 gacttgccct ccacttctct ggcgatgtta tcgatatcgt cccttccgta cttctcgtta
     4861 gccttgatga attggttgaa gtcacgctta gtccagttgg tgaagccctg agtcagcaac
     4921 ttctcctttt cctcggtttc ctctggtgtg agaggttcag caccatcgat cttcttttgt
     4981 tcctccctct gagccagggc agggtttggg atgtctgggt ttctcgggac cttgtaaccg
     5041 atggtcttac ggtagtagag gatctccttt tcgagcagct cgaacagcct aggggggaag
     5101 aattggaagt cctgcacgtt tggctgcttt ggtggtcttg gagccttagg gatcttgggc
     5161 tcagagacgc gcagagcttc acggaagtaa gcgtccacgg cgtagttagc ctttctctcc
     5221 ctcttagggg gctcgatcca ttcgaccatg ccgagctttt gcttttcgcg gtaatcctcg
     5281 ccttcgaact tgtacaggga ctgctcgatg tccatgcgga agttacgcag gctagattct
     5341 cccatcttct gcaagcgctc gttcatttca gcggtcttct tctcgccacg ttccaagatg
     5401 gttgtgatat cctcgtcggt gagctctgat tccttgctgg cgaagacgtg tgtagcaccg
     5461 tgacggatca tctgcagcat ttcctccttg gccaacttgt tggattgctg atcgatcagg
     5521 cgtccttgct ggataacgat cgagtccaga cgcaacttga tctcagctct ttccacgatc
     5581 ctttcctcaa ctgtgttgtc agtgatgagt ctgaacaccc taactggctt cttctggccg
     5641 atgcggtggg cacgatccat agcttgcagg tccacctgag ggttccaatc tgagtcgtac
     5701 aagatcacaa cgtcagcgct ggccaggttg atacccaaac ctccagcgcg ggtagacagc
     5761 atgaagatga acttggacga gtttggggcg ttgaaagcct cgatggcttc ctcacgttcc
     5821 tcgtgagggg tctgaccgtc gaggcgacag tactcgtatc cacgccacat gcagtaatct
     5881 tccaagatgt ccaagagtct tgtcatttgg ctgaagatga gaaccctaga tccctgctcc
     5941 ttgagcttgg ccagcaactt gtccaggacc accatcttgc cggagttcga gacgatgtgc
     6001 tcatcagtgg tgtacggtgg accgggttca gctccgtcga acaggtacgg gtggttacag
     6061 cacttgcgga gttgcatcag gatgttgagc agacgcatct tgtccatctt tcctgagctg
     6121 ttcaagacat cgatgtcctt catgaggatc ttagtgtacc actccctctg catcttgctg
     6181 aggcccaggt agatcttgat ctccttctta gggggcagag acttttccac gtctgtcttg
     6241 attctcctca agaggaaagg cttgaggaca gcgtgcaggc gctccaccaa cttctggtca
     6301 ccgaggcagt tcttagtatc gaaccaggag tcgaaatcgt cggccgagtt gaagacgtcg
     6361 ggcagcaaga agttgagcag agcccacaac tcgtggaggt tgttctgcag tggtgttcca
     6421 gtcaagagca gtctgttggt gctcttgaac tccctcacga tttcagacaa cttggacttc
     6481 tcgttcttga ttctgtgggc ttcgtcgatg accaggtaac gccagtggaa cttcttgaaa
     6541 acagacttct ccttgatgac catttcgtag gaagtgacac acacatccca ttcaccgggc
     6601 atcatttcgt cgcggatgaa agcggcacga gcatccttgt ctccaacgaa gcagatgact
     6661 ctcagcgatg gcacccaacg cttgaactcg ttcatccagt tgtggagggt tgacttcgga
     6721 accaggacca tgtgcggtcc tgggatgttg cggtagtgct tcaggtagcc caagagggcg
     6781 atggtttgga gtgtctttcc caggcccatc tcatcagcca agatgccgtt gacaccgttt
     6841 tcgtacagag agatcaacca gttgaggcct ctgatctggt agtccctcag ggggccaccc
     6901 ttgacgtatg acgggctcac ctcgaagcgg atgcacacgt tggatgtctt acgagactcg
     6961 gacagcaatt cctcgtcttc ctcttgttca gtgcgacggt gacggtaatc gccagccgag
     7021 atcagtgact gcttctcgtc cttcttgatg cgaggacgac ccaacttcat gttgagcggg
     7081 ctagttggag acttttgggc tgagggctgg atgaagtgag cgaacagctc ggtctgcttg
     7141 agcaggaatt cgaatctctt agccctatcg gccttcatct tttcctcgta ctctgggtcc
     7201 atctcctttt cgctcttagg ggccttagcg gccaacttga gttggaaaga ggacacgttc
     7261 ttctccttct tcttctcgcc cttttcagta gcggcggtag cttctgtagc agcagcagca
     7321 gctccttcct cctgcgaggt tgagggaccc ggttgttcat cttcgataac gacgatagta
     7381 gcggtggcat cagcagcagc gacggtagca gcgacggcag cagtgtcttg ttccatcccg
     7441 ggtgatcaag tcttcgtcga gtgattgtaa ataaaatgta atttacagta tagtatttta
     7501 attaatatac aaatgatttg ataataattc ttatttaact ataatatatt gtgttgggtt
     7561 gaattaaagg tccgtatact ccggaatatt aatagatcat ggagataatt aaaatgataa
     7621 ccatctcgca aataaataag tattttactg ttttcgtaac agttttgtaa taaaaaaacc
     7681 tataaatatt ccggattatt cataccgtcc caccatcggg cgcggatcgg gatggccgac
     7741 aaggaagcag ccttcgacga cgcagtggaa gaacgagtga tcaacgagga atacaaaata
     7801 tggaaaaaga acaccccttt tctttatgat ttggtgatga cccatgctct ggagtggccc
     7861 agcctaactg cccagtggct tccagatgta accagaccag aagggaaaga tttcagcatt
     7921 catcgacttg tcctggggac acacacatcg gatgaacaaa accatcttgt tatagccagt
     7981 gtgcagctcc ctaatgatga tgctcagttt gatgcgtcac actacgacag tgagaaagga
     8041 gaatttggag gttttggttc agttagtgga aaaattgaaa tagaaatcaa gatcaaccat
     8101 gaaggagaag taaacagggc ccgttatatg ccccagaacc cttgtatcat cgcaacaaag
     8161 actccttcca gtgatgttct tgtctttgac tatacaaaac atccttctaa accagatcct
     8221 tctggagagt gcaacccaga cttgcgtctc cgtggacatc agaaggaagg ctatgggctt
     8281 tcttggaacc caaatctcag tgggcactta cttagtgctt cagatgacca taccatctgc
     8341 ctgtgggaca tcagtgccgt tccaaaggag ggaaaagtgg tagatgcgaa gaccatcttt
     8401 acagggcata cggcagtagt agaagatgtt tcctggcatc tactccatga gtctctgttt
     8461 gggtcagttg ctgatgatca gaaacttatg atttgggata ctcgttcaaa caatacttcc
     8521 aaaccaagcc actcagttga tgctcacact gctgaagtga actgcctttc tttcaatcct
     8581 tatagtgagt tcattcttgc cacaggatca gctgacaaga ctgttgcctt gtgggatctg
     8641 agaaatctga aacttaagtt gcattccttt gagtcacata aggatgaaat attccaggtt
     8701 cagtggtcac ctcacaatga gactatttta gcttccagtg gtactgatcg cagactgaat
     8761 gtctgggatt taagtaaaat tggagaggaa caatccccag aagatgcaga agacgggcca
     8821 ccagagttgt tgtttattca tggtggtcat actgccaaga tatctgattt ctcctggaat
     8881 cccaatgaac cttgggtgat ttgttctgta tcagaagaca atatcatgca agtgtggcaa
     8941 atggcagaga acatttataa tgatgaagac cctgaaggaa gcgtggatcc agaaggacaa
     9001 gggtcctagg catgccccag cttgtcgaga agtactagag gatcataatc agccatacca
     9061 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
     9121 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
     9181 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     9241 gtttgtccaa actcatcaat gtatcttatc atgtctggat ctgatcactg cttgagccta
     9301 ggagatccga accagataag tgaaatctag ttccaaacta ttttgtcatt tttaattttc
     9361 gtattagctt acgacgctac acccagttcc catctatttt gtcactcttc cctaaataat
     9421 ccttaaaaac tccatttcca cccctcccag ttcccaacta ttttgtccgc ccacagcggg
     9481 gcatttttct tcctgttatg tttttaatca aacatcctgc caactccatg tgacaaaccg
     9541 tcatcttcgg ctacttt
//
