LOCUS       sequence-197775-        7087 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  split YFP. Plant expression of p35S:TN3-YFPn.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7087)
  TITLE     Robatzek Lab plasmids
REFERENCE   2  (bases 1 to 7087)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p35S:TN3-YFPn
FEATURES             Location/Qualifiers
     source          1..7087
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      20..608
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     509..528
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    920..944
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(1038..1290)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(1310..1861)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(1903..2082)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        2551..2895
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     2839..2859
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     primer_bind     2897..2913
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     2898..2914
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2930..2954
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4278..4302)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(4310..4312,4313..4315,4316..4774)
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMA"
     primer_bind     complement(4355..4376)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4616..4635)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    5066..5090
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      5202..5911
                     /label=oriV
                     /note="incP origin of replication"
     promoter        5984..6075
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6076..6144,6145..6936)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6294..6313)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
       61 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
      121 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
      181 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
      241 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
      301 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
      361 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
      421 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
      481 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
      541 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
      601 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
      661 ctcacatgga ctctagctag aggatcacag gcagcaacgc tctgtcatcg ttacaatcaa
      721 catgctaccc tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc
      781 cgaatagcat cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc
      841 cgtcccggac tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg
      901 ggagctgttg gctggctggt ggcaggatat attgtggtgt aaacaaattg acgcttagac
      961 aacttaataa cacattgcgg acgtttttaa tgatcgaata ctaacgtctc taccagatat
     1021 cagcttgcat gccggtcgat ctagtaacat agatgacacc gcgcgcgata atttatccta
     1081 gtttgcgcgc tatattttgt tttctatcgc gtattaaatg tataattgcg ggactctaat
     1141 cataaaaacc catctcataa ataacgtcat gcattacatg ttaattatta catgcttaac
     1201 gtaattcaac agaaattata tgataatcat cgcaagaccg gcaacaggat tcaatcttaa
     1261 gaaactttat tgccaaatgt ttgaacgatc tgcttgactc taggggtcat cagatttcgg
     1321 tgacgggcag gaccggacgg ggcggcaccg gcaggctgaa gtccagctgc cagaaaccca
     1381 cgtcatgcca gttcccgtgc ttgaagccgg ccgcccgcag catgccgcgg ggggcatatc
     1441 cgagcgcctc gtgcatgcgc acgctcgggt cgttgggcag cccgatgaca gcgaccacgc
     1501 tcttgaagcc ctgtgcctcc agggacttca gcaggtgggt gtagagcgtg gagcccagtc
     1561 ccgtccgctg gtggcggggg gagacgtaca cggtcgactc ggccgtccag tcgtaggcgt
     1621 tgcgtgcctt ccagggaccc gcgtaggcga tgccggcgac ctcgccgtcc acctcggcga
     1681 cgagccaggg atagcgctcc cgcagacgga cgaggtcgtc cgtccactcc tgcggttcct
     1741 gcggctcggt acggaagttg accgtgcttg tctcgatgta gtggttgacg atggtgcaga
     1801 ccgccggcat gtccgcctcg gtggcacggc ggatgtcggc cgggcgtcgt tctgggctca
     1861 tggtagatcc cctcgatcga gttgagagtg aatatgagac tctaattgga taccgagggg
     1921 aatttatgga acgtcagtgg agcatttttg acaagaaata tttgctagct gatagtgacc
     1981 ttaggcgact tttgaacgcg caataatggt ttctgacgta tgtgcttagc tcattaaact
     2041 ccagaaaccc gcggctcagt ggctccttca acgttgcggt tctgtcagtt ccaaacgtaa
     2101 aacggcttgt cccgcgtcat cggcgggggt cataacgtga ctcccttaat tctccgctca
     2161 tgatcagatt gtcgtttccc gccttcggtt tgggcgcgcc cggttcagaa gaccagaggg
     2221 ctattgagac ttttcaacaa agggtaatat cgggaaacct cctcggattc cattgcccag
     2281 ctatctgtca cttcatcgaa aggacagtag aaaaggaaga tggcttctac aaatgccatc
     2341 attgcgataa aggaaaggct atcgttcaag atgcctctac cgacagtggt cccaaagatg
     2401 gacccccacc cacgaggaac atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc
     2461 aagtggattg atgtgataca tggtggagca cgacactctc gtctactcca agaatatcaa
     2521 agatacagtc tcagaagacc agagggctat tgagactttt caacaaaggg taatatcggg
     2581 aaacctcctc ggattccatt gcccagctat ctgtcacttc atcgaaagga cagtagaaaa
     2641 ggaagatggc ttctacaaat gccatcattg cgataaagga aaggctatcg ttcaagatgc
     2701 ctctaccgac agtggtccca aagatggacc cccacccacg aggaacatcg tggaaaaaga
     2761 agacgttcca accacgtctt caaagcaagt ggattgatgt gatatctcca ctgacgtaag
     2821 ggatgacgca caatcccact atccttcgca agacccttcc tctatataag gaagttcatt
     2881 tcatttggag aggacctcga ggtcgacggt atcgataagc ttcccatcaa caagtttgta
     2941 caaaaaagca ggctccgcgg ccgccccctt caccatggct gcatctactt cttcttcttc
     3001 ttcatcatca tcttcttctt tatcttctcc ttcaccttgc acatggagat acatgacttt
     3061 cacgagcttc catggaccag atgtccgcaa cacatttctt agtcatttga ggaaacagtt
     3121 taacaccaat ggaattacga tgtttgatga tcaaaggatg gagagaagcc aaacccttgc
     3181 ccctactctc acgcaggcga ttagagaatc gaagatctac atcgtcttgc tctcgaagaa
     3241 ctatgcttca tcgagctggt gtcttgatga gctattggag attttaaact gcaaagaaaa
     3301 aagaggacag agagtgatga caatcttcta cggcgtaaat ccatctgatg tgcggaaaca
     3361 aaccggagaa tttggaatcg ctttcaacga aacttgtgct cgtaaaacag aggaggagag
     3421 gagaaaatgg agccacgctt tgacctgtgt gggcaacata accggagtac acgtccaaga
     3481 cagggacgat gaggcaaaca tgatcgagaa aattgcaact gatgtctcag aaaaactgaa
     3541 tgctacagag tcaaaggatt ttgatgaaat ggtgggaatt aaagctcatt tgacgaaaat
     3601 tgagtctttg ctatctttag attatgataa agttaagatc gttgggatct ccggtcctgc
     3661 aggcattggt aagagtacca ttgccagagc cttacataac ctactctcta gcagttttca
     3721 tcttagttgt tttatggaaa accttattag ccaaagcaac ccccatagtt ctctagagta
     3781 tagttcgaaa ttgagtttac aagagcaact tctctcacaa gttttgaacg aaaaggacat
     3841 taggatacgc catttaggtg cgatacaaga aaggctacac gaccagaggg ttcttatcat
     3901 tcttgatgac gtgacgagtc tagagcaact agaggttttg gctaatatca agtggtatgg
     3961 tcctggaagt aggatcatag taatcactaa aaagaaagat attttggtgc aacatggtat
     4021 ctgtgatata taccatgtgg gttttccaac agatgcagat gctctaaaga tcttttgtct
     4081 atctgcttat agacaaacct ctccacctga tggatctatg aaaattcatg agtgtgaaat
     4141 gtttataaaa atttgtggca atcttccatt gcatctacat gttttggggt cggcgcttcg
     4201 gggaaggagt tatggcagag tgcagagttt gtgtaatcta gtgtctctgg ctgattttgt
     4261 aaagggtggg cgcgccgacc cagctttctt gtacaaagtg gttgatggga tggtgagcaa
     4321 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
     4381 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
     4441 cctgaagctg atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
     4501 cctgggctac ggcctgcagt gcttcgcccg ctaccccgac cacatgaagc agcacgactt
     4561 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
     4621 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
     4681 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
     4741 caactacaac agccacaacg tctatatcat ggcctaatct agagtccgca aaaatcacca
     4801 gtctctctct acaaatctat ctctctctat ttttctccag aataatgtgt gagtagttcc
     4861 cagataaggg aattagggtt cttatagggt ttcgctcatg tgttgagcat ataagaaacc
     4921 cttagtatgt atttgtattt gtaaaatact tctatcaata aaatttctaa ttcctaaaac
     4981 caaaatccag tgaccgggcg gccgccaccg cggtggaggg ggatcagatt gtcgtttccc
     5041 gccttcagtt taaactatca gtgtttgaca ggatatattg gcgggtaaac ctaagagaaa
     5101 agagcgttta ttagaataat cggatattta aaagggcgtg aaaaggttta tccgttcgtc
     5161 catttgtatg tgtacatcac cgacgagcaa ggcaagaccg agcgcctttc cgacgctcac
     5221 cgggctggtt gccctcgccg ctgggctggc ggccgtctat ggccctgcaa acgcgccaga
     5281 aacgccgtcg aagccgtgtg cgagacaccg gccgccggcg ttgtggatac ctcgcggaaa
     5341 acttggccct cactgacaga tgaggggcgg acgttgacac ttgaggggcc gactcacccg
     5401 gcgcggcgtt gacagatgag gggcaggctc gatttcggcc ggcgacgtgg agctggccag
     5461 cctcgcaaat cggcgaaaac gcctgatttt acgcgagttt cccacagatg atgtggacaa
     5521 gcctggggat aagtgccctg cggtattgac acttgagggg cgcgactact gacagatgag
     5581 gggcgcgatc cttgacactt gaggggcaga gtgctgacag atgaggggcg cacctattga
     5641 catttgaggg gctgtccaca ggcagaaaat ccagcatttg caagggtttc cgcccgtttt
     5701 tcggccaccg ctaacctgtc ttttaacctg cttttaaacc aatatttata aaccttgttt
     5761 ttaaccaggg ctgcgccctg tgcgcgtgac cgcgcacgcc gaaggggggt gccccccctt
     5821 ctcgaaccct cccggcccgc taacgcgggc ctcccatccc cccaggggct gcgcccctcg
     5881 gccgcgaacg gcctcacccc aaaaatggca gcgctggcag tccttgccat tgccgggatc
     5941 ggggcagtaa cgggatgggc gatcagcccg aaagctaccc ctatttgttt atttttctaa
     6001 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     6061 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     6121 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     6181 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     6241 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     6301 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     6361 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     6421 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     6481 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     6541 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     6601 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     6661 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     6721 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     6781 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     6841 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     6901 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     6961 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     7021 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     7081 cccgtag
//
