LOCUS       sequence-197796-       13013 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses sgRNA targeting mouse p27.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13013)
  AUTHORS   Song W, Hwang Y, Youngblood VM, Cook RS, Balko JM, Chen J, 
            Brantley-Sieders DM
  TITLE     Targeting EphA2 impairs cell cycle progression and growth of 
            basal-like/triple-negative breast cancers.
  JOURNAL   Oncogene. 2017 Jun 5. doi: 10.1038/onc.2017.170.
  PUBMED    28581527
REFERENCE   2  (bases 1 to 13013)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Oncogene. 
            2017 Jun 5. doi: 10.1038/onc.2017.170."
COMMENT     Sequence Label: sgp27#1
FEATURES             Location/Qualifiers
     source          1..13013
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     202..222
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    321..909
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(374..394)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(792..803)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             981..1214
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(1240..1257)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1246..1470
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      1516..1944
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1603..1622)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1813..1834
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(1953..1973)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        1958..2287
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2138..2273
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2200..2219
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        2335..2382
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             2401..2775
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    2905..3026
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2942..2961)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2996..3015
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3075..3091)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3075..3091)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3088..3110)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3099..3115
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3123..3129,3130..3147,3148..3153))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3168..3189
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3306..3323)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3477..4065)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3557..3576)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4236..5027,5028..5096))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4859..4878
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5097..5201)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(5276..5295)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        5467..5846
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5848..6046
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6001..6021
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             6064..6244
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6291..6416
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6909..7142
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7327..7371
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7669..7786
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7837..8085
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     7837..7857
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     8008..8027
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        8107..8182
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        8244..8455
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      8474..8483
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8480..12583
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             8480..12583
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             12584..12631
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             12632..12655
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             12665..12721
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(12722..13013,1..305)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
ORIGIN
        1 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca
       61 aggagcccgc gtggttcctg gccaccgtcg gagtctcgcc cgaccaccag ggcaagggtc
      121 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct
      181 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca
      241 ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg
      301 cctgaacgcg ttaagtcgac aatcaacctc tggattacaa aatttgtgaa agattgactg
      361 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
      421 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
      481 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
      541 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
      601 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
      661 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
      721 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
      781 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
      841 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
      901 cctccccgcg tcgactttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
      961 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct
     1021 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     1081 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     1141 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     1201 gaaaatctct agcagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt
     1261 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
     1321 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
     1381 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
     1441 ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct
     1501 ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     1561 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     1621 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     1681 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     1741 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     1801 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     1861 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     1921 tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa
     1981 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     2041 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     2101 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     2161 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     2221 cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     2281 ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcagc acgtgttgac
     2341 aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag gaactaaacc
     2401 atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc
     2461 gagttctgga ccgaccggct cgggttctcc cgggacttcg tggaggacga cttcgccggt
     2521 gtggtccggg acgacgtgac cctgttcatc agcgcggtcc aggaccaggt ggtgccggac
     2581 aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag
     2641 gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca tgaccgagat cggcgagcag
     2701 ccgtgggggc gggagttcgc cctgcgcgac ccggccggca actgcgtgca cttcgtggcc
     2761 gaggagcagg actgacacgt gctacgagat ttcgattcca ccgccgcctt ctatgaaagg
     2821 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
     2881 atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa
     2941 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     3001 ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc
     3061 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     3121 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     3181 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     3241 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     3301 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     3361 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     3421 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     3481 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     3541 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     3601 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     3661 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     3721 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     3781 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     3841 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     3901 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     3961 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     4021 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     4081 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     4141 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     4201 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     4261 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     4321 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     4381 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     4441 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     4501 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     4561 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     4621 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     4681 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     4741 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     4801 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     4861 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     4921 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     4981 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     5041 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     5101 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     5161 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     5221 ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa
     5281 tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg
     5341 ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca
     5401 tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata
     5461 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
     5521 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
     5581 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
     5641 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
     5701 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
     5761 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
     5821 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
     5881 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
     5941 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
     6001 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt
     6061 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     6121 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     6181 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     6241 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
     6301 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
     6361 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
     6421 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
     6481 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
     6541 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
     6601 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
     6661 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
     6721 agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga
     6781 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
     6841 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
     6901 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
     6961 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
     7021 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
     7081 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
     7141 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
     7201 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
     7261 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
     7321 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
     7381 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
     7441 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
     7501 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga
     7561 atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatcg
     7621 gcactgcgtg cgccaattct gcagacaaat ggcagtattc atccacaatt ttaaaagaaa
     7681 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
     7741 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
     7801 ggacagcaga gatccagttt ggttaattaa ggtaccgagg gcctatttcc catgattcct
     7861 tcatatttgc atatacgata caaggctgtt agagagataa ttagaattaa tttgactgta
     7921 aacacaaaga tattagtaca aaatacgtga cgtagaaagt aataatttct tgggtagttt
     7981 gcagttttaa aattatgttt taaaatggac tatcatatgc ttaccgtaac ttgaaagtat
     8041 ttcgatttct tggctttata tatcttgtgg aaaggacgaa acaccgtcaa acgtgagagt
     8101 gtctaagttt tagagctaga aatagcaagt taaaataagg ctagtccgtt atcaacttga
     8161 aaaagtggca ccgagtcggt gcttttttga attcgctagc taggtcttga aaggagtggg
     8221 aattggctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt
     8281 ggggggaggg gtcggcaatt gatccggtgc ctagagaagg tggcgcgggg taaactggga
     8341 aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag
     8401 tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacaggaccg
     8461 gttctagagc gctgccacca tggacaagaa gtacagcatc ggcctggaca tcggcaccaa
     8521 ctctgtgggc tgggccgtga tcaccgacga gtacaaggtg cccagcaaga aattcaaggt
     8581 gctgggcaac accgaccggc acagcatcaa gaagaacctg atcggagccc tgctgttcga
     8641 cagcggcgaa acagccgagg ccacccggct gaagagaacc gccagaagaa gatacaccag
     8701 acggaagaac cggatctgct atctgcaaga gatcttcagc aacgagatgg ccaaggtgga
     8761 cgacagcttc ttccacagac tggaagagtc cttcctggtg gaagaggata agaagcacga
     8821 gcggcacccc atcttcggca acatcgtgga cgaggtggcc taccacgaga agtaccccac
     8881 catctaccac ctgagaaaga aactggtgga cagcaccgac aaggccgacc tgcggctgat
     8941 ctatctggcc ctggcccaca tgatcaagtt ccggggccac ttcctgatcg agggcgacct
     9001 gaaccccgac aacagcgacg tggacaagct gttcatccag ctggtgcaga cctacaacca
     9061 gctgttcgag gaaaacccca tcaacgccag cggcgtggac gccaaggcca tcctgtctgc
     9121 cagactgagc aagagcagac ggctggaaaa tctgatcgcc cagctgcccg gcgagaagaa
     9181 gaatggcctg ttcggaaacc tgattgccct gagcctgggc ctgaccccca acttcaagag
     9241 caacttcgac ctggccgagg atgccaaact gcagctgagc aaggacacct acgacgacga
     9301 cctggacaac ctgctggccc agatcggcga ccagtacgcc gacctgtttc tggccgccaa
     9361 gaacctgtcc gacgccatcc tgctgagcga catcctgaga gtgaacaccg agatcaccaa
     9421 ggcccccctg agcgcctcta tgatcaagag atacgacgag caccaccagg acctgaccct
     9481 gctgaaagct ctcgtgcggc agcagctgcc tgagaagtac aaagagattt tcttcgacca
     9541 gagcaagaac ggctacgccg gctacattga cggcggagcc agccaggaag agttctacaa
     9601 gttcatcaag cccatcctgg aaaagatgga cggcaccgag gaactgctcg tgaagctgaa
     9661 cagagaggac ctgctgcgga agcagcggac cttcgacaac ggcagcatcc cccaccagat
     9721 ccacctggga gagctgcacg ccattctgcg gcggcaggaa gatttttacc cattcctgaa
     9781 ggacaaccgg gaaaagatcg agaagatcct gaccttccgc atcccctact acgtgggccc
     9841 tctggccagg ggaaacagca gattcgcctg gatgaccaga aagagcgagg aaaccatcac
     9901 cccctggaac ttcgaggaag tggtggacaa gggcgcttcc gcccagagct tcatcgagcg
     9961 gatgaccaac ttcgataaga acctgcccaa cgagaaggtg ctgcccaagc acagcctgct
    10021 gtacgagtac ttcaccgtgt ataacgagct gaccaaagtg aaatacgtga ccgagggaat
    10081 gagaaagccc gccttcctga gcggcgagca gaaaaaggcc atcgtggacc tgctgttcaa
    10141 gaccaaccgg aaagtgaccg tgaagcagct gaaagaggac tacttcaaga aaatcgagtg
    10201 cttcgactcc gtggaaatct ccggcgtgga agatcggttc aacgcctccc tgggcacata
    10261 ccacgatctg ctgaaaatta tcaaggacaa ggacttcctg gacaatgagg aaaacgagga
    10321 cattctggaa gatatcgtgc tgaccctgac actgtttgag gacagagaga tgatcgagga
    10381 acggctgaaa acctatgccc acctgttcga cgacaaagtg atgaagcagc tgaagcggcg
    10441 gagatacacc ggctggggca ggctgagccg gaagctgatc aacggcatcc gggacaagca
    10501 gtccggcaag acaatcctgg atttcctgaa gtccgacggc ttcgccaaca gaaacttcat
    10561 gcagctgatc cacgacgaca gcctgacctt taaagaggac atccagaaag cccaggtgtc
    10621 cggccagggc gatagcctgc acgagcacat tgccaatctg gccggcagcc ccgccattaa
    10681 gaagggcatc ctgcagacag tgaaggtggt ggacgagctc gtgaaagtga tgggccggca
    10741 caagcccgag aacatcgtga tcgaaatggc cagagagaac cagaccaccc agaagggaca
    10801 gaagaacagc cgcgagagaa tgaagcggat cgaagagggc atcaaagagc tgggcagcca
    10861 gatcctgaaa gaacaccccg tggaaaacac ccagctgcag aacgagaagc tgtacctgta
    10921 ctacctgcag aatgggcggg atatgtacgt ggaccaggaa ctggacatca accggctgtc
    10981 cgactacgat gtggaccata tcgtgcctca gagctttctg aaggacgact ccatcgacaa
    11041 caaggtgctg accagaagcg acaagaaccg gggcaagagc gacaacgtgc cctccgaaga
    11101 ggtcgtgaag aagatgaaga actactggcg gcagctgctg aacgccaagc tgattaccca
    11161 gagaaagttc gacaatctga ccaaggccga gagaggcggc ctgagcgaac tggataaggc
    11221 cggcttcatc aagagacagc tggtggaaac ccggcagatc acaaagcacg tggcacagat
    11281 cctggactcc cggatgaaca ctaagtacga cgagaatgac aagctgatcc gggaagtgaa
    11341 agtgatcacc ctgaagtcca agctggtgtc cgatttccgg aaggatttcc agttttacaa
    11401 agtgcgcgag atcaacaact accaccacgc ccacgacgcc tacctgaacg ccgtcgtggg
    11461 aaccgccctg atcaaaaagt accctaagct ggaaagcgag ttcgtgtacg gcgactacaa
    11521 ggtgtacgac gtgcggaaga tgatcgccaa gagcgagcag gaaatcggca aggctaccgc
    11581 caagtacttc ttctacagca acatcatgaa ctttttcaag accgagatta ccctggccaa
    11641 cggcgagatc cggaagcggc ctctgatcga gacaaacggc gaaaccgggg agatcgtgtg
    11701 ggataagggc cgggattttg ccaccgtgcg gaaagtgctg agcatgcccc aagtgaatat
    11761 cgtgaaaaag accgaggtgc agacaggcgg cttcagcaaa gagtctatcc tgcccaagag
    11821 gaacagcgat aagctgatcg ccagaaagaa ggactgggac cctaagaagt acggcggctt
    11881 cgacagcccc accgtggcct attctgtgct ggtggtggcc aaagtggaaa agggcaagtc
    11941 caagaaactg aagagtgtga aagagctgct ggggatcacc atcatggaaa gaagcagctt
    12001 cgagaagaat cccatcgact ttctggaagc caagggctac aaagaagtga aaaaggacct
    12061 gatcatcaag ctgcctaagt actccctgtt cgagctggaa aacggccgga agagaatgct
    12121 ggcctctgcc ggcgaactgc agaagggaaa cgaactggcc ctgccctcca aatatgtgaa
    12181 cttcctgtac ctggccagcc actatgagaa gctgaagggc tcccccgagg ataatgagca
    12241 gaaacagctg tttgtggaac agcacaagca ctacctggac gagatcatcg agcagatcag
    12301 cgagttctcc aagagagtga tcctggccga cgctaatctg gacaaagtgc tgtccgccta
    12361 caacaagcac cgggataagc ccatcagaga gcaggccgag aatatcatcc acctgtttac
    12421 cctgaccaat ctgggagccc ctgccgcctt caagtacttt gacaccacca tcgaccggaa
    12481 gaggtacacc agcaccaaag aggtgctgga cgccaccctg atccaccaga gcatcaccgg
    12541 cctgtacgag acacggatcg acctgtctca gctgggaggc gacaagcgac ctgccgccac
    12601 aaagaaggct ggacaggcta agaagaagaa agattacaaa gacgatgacg ataagggatc
    12661 cggcgcaaca aacttctctc tgctgaaaca agccggagat gtcgaagaga atcctggacc
    12721 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg
    12781 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg
    12841 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
    12901 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
    12961 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tga
//
