LOCUS       sequence-197797-       13886 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  fluorescent tagging. Plant expression of EFR WT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13886)
  TITLE     Robatzek Lab plasmids
REFERENCE   2  (bases 1 to 13886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: EFR WT
FEATURES             Location/Qualifiers
     source          1..13886
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        588..933
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     876..896
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    945..969
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4099..4123)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4146..4856
                     /codon_start=1
                     /gene="mgfp5"
                     /product="GFP with folding enhancement mutations"
                     /label=mgfp5
                     /note="suitable for expression in plants due to removal of 
                     a cryptic intron"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKAN
                     FKTRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(4173..4201)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     4796..4816
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             4863..4880
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      4938..5645
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     primer_bind     complement(5670..5687)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5670..5686)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5679..5701)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    5889..5913
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7214..7843
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             8272..9345
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      9411..9605
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     misc_feature    9949..10089
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     10004..10023
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(10104..10121)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10275..10863)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10355..10374)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(10950..11744)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    12169..12193
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(12279..12531)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     CDS             complement(12541..13092)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(13098..13478)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
ORIGIN
        1 cggagattac aatggacgat ttcctctatc tttacgatct aggaaggaag ttcgaaggtg
       61 aaggtgacga cactatgttc accactgata atgagaaggt tagcctcttc aatttcagaa
      121 agaatgctga cccacagatg gttagagagg cctacgcagc aggtctcatc aagacgatct
      181 acccgagtaa caatctccag gagatcaaat accttcccaa gaaggttaaa gatgcagtca
      241 aaagattcag gactaattgc atcaagaaca cagagaaaga catatttctc aagatcagaa
      301 gtactattcc agtatggacg attcaaggct tgcttcataa accaaggcaa gtaatagaga
      361 ttggagtctc taaaaaggta gttcctactg aatctaaggc catgcatgga gtctaagatt
      421 caaatcgagg atctaacaga actcgccgtg aagactggcg aacagttcat acagagtctt
      481 ttacgactca atgacaagaa gaaaatcttc gtcaacatgg tggagcacga cactctggtc
      541 tactccaaaa atgtcaaaga tacagtctca gaagaccaaa gggctattga gacttttcaa
      601 caaaggataa tttcgggaaa cctcctcgga ttccattgcc cagctatctg tcacttcatc
      661 gaaaggacag tagaaaagga aggtggctcc tacaaatgcc atcattgcga taaaggaaag
      721 gctatcattc aagatctctc tgccgacagt ggtcccaaag atggaccccc acccacgagg
      781 agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga ttgatgtgac
      841 atctccactg acgtaaggga tgacgcacaa tcccactatc cttcgcaaga cccttcctct
      901 atataaggaa gttcatttca tttggagagg acacgctcga gatcacaagt ttgtacaaaa
      961 aagcaggctc cgcggccgcc cccttcacca tgaagctgtc cttttcactt gttttcaatg
     1021 ctctcacgtt gcttcttcaa gtttgcatct ttgctcaagc caggttttct aatgagactg
     1081 atatgcaagc tttgcttgag ttcaagtctc aagtttctga aaacaacaag agagaggtct
     1141 tggcttcatg gaatcactcc tccccatttt gtaattggat tggagtcaca tgtggccgca
     1201 ggagagaaag agttataagt ttgaaccttg gaggattcaa gttaaccggt gtgatctcac
     1261 cctccattgg taatctctcc tttcttagat tacttaatct tgcagacaac tcttttggaa
     1321 gtaccatccc tcaaaaggtg ggaaggctat ttaggcttca gtacttgaac atgagctata
     1381 atcttctcga aggaaggatt ccgtctagtc tttctaactg ctctagactg tcgaccgttg
     1441 atttatcgtc aaaccatctt ggacatggtg ttccttcaga actaggttca ctttctaagc
     1501 ttgccattct ggatcttagc aaaaacaacc ttactggaaa ttttcctgca tctttaggaa
     1561 acttgacgtc acttcagaag cttgactttg catataacca gatgagaggt gagattccag
     1621 acgaagtagc tagattgact caaatggtgt ttttccaaat agcactgaat agtttttcag
     1681 gtggttttcc tcctgcattg tacaacatct cctctcttga gtctctatct ctagctgaca
     1741 atagcttttc gggtaatctt agggctgatt ttggttatct tctaccaaat ctaagaagac
     1801 ttcttttggg aacaaatcag ttcactggag ctattcccaa aacacttgcc aatatctcaa
     1861 gccttgaaag gtttgatatc tcatctaatt acctgtctgg tagtatccct ttgagctttg
     1921 gaaagttacg taatctgtgg tggttaggga ttcgtaataa ctctcttgga aataactcgt
     1981 ccagtggtct tgaatttatt ggggctgtgg cgaactgcac tcaattagag tacttagatg
     2041 ttggttacaa tagacttgga ggtgagcttc ctgcttctat agccaatctg tccactacat
     2101 tgactagtct gttccttgga caaaatctta tctctggaac cattcctcat gacatcggga
     2161 atcttgtaag cctgcaagaa ctcagcttag aaacaaatat gttgagtgga gaacttcccg
     2221 tctctttcgg gaagcttttg aacttgcagg ttgtggatct gtattcaaat gcaatatcgg
     2281 gggaaatacc atcttatttt ggcaacatga ctcggttgca gaagctccat ttgaatagca
     2341 atagtttcca cggaagaatc cctcagagtc ttggacgttg tcgatacttg ctagacctgt
     2401 ggatggatac aaataggttg aatgggacta tacctcagga aatactgcaa attccatccc
     2461 tcgcttacat agatttgtca aacaatttct tgacaggcca ttttccagaa gaagttggaa
     2521 agttagaact tcttgttgga ctaggtgctt cgtacaacaa attatcagga aagatgccac
     2581 aagctatagg gggttgtctt tcgatggaat ttctctttat gcaaggaaat tcgtttgacg
     2641 gagccattcc agatataagc cggttggtaa gcctaaagaa tgttgacttc tccaacaaca
     2701 atctctctgg ccggatacct cgatatctgg ccagtcttcc ttcgctgcga aatctgaatc
     2761 tttctatgaa caagtttgag ggaagggtgc caacaacagg agtgtttcga aatgctacag
     2821 cagtttctgt ttttggtaac acaaatattt gcggaggcgt ccgagaaatg caactaaagc
     2881 catgcattgt acaggcatct ccaaggaaga gaaagcctct gtcagttaga aagaaagttg
     2941 tcagtggtat ttgtataggt atagcttcgc ttttgttaat cataattgtg gcttctctgt
     3001 gttggttcat gaagaggaaa aagaaaaaca atgccagtga tggtaaccca tctgattcta
     3061 ctactttggg gatgttccat gagaaggtaa gttatgaaga gcttcatagt gcaacaagtc
     3121 gcttctcttc aaccaatttg attggttcag gcaatttcgg taatgtgttt aaaggattgc
     3181 ttggccctga gaataaactc gtcgcggtta aagttttgaa cctcctaaag catggagcga
     3241 cgaaaagctt tatggcggaa tgtgaaacct tcaagggtat acgacatcgt aaccttgtaa
     3301 aactgataac ggtttgttca agccttgatt ccgagggaaa tgatttcaga gctctggtct
     3361 atgagttcat gccaaaagga agtctggata tgtggctgca gctagaagat ctggaaaggg
     3421 taaacgatca ctcgagatct ttaacacccg cagagaaact caacatagca atagatgtgg
     3481 cttcagcttt ggagtatctg cacgttcatt gtcatgaccc tgtagctcac tgtgatatta
     3541 agccaagcaa cattcttcta gacgatgatc tgactgctca tgttagtgac tttggtttgg
     3601 ctcagctcct ctataaatac gatcgagaat cctttctaaa ccagtttagt tctgctggtg
     3661 tcagaggcac cattggctat gccgcgccag agtatggaat gggaggccaa ccatcaatac
     3721 aaggagatgt gtacagcttc ggaattctac ttttggagat gtttagtgga aagaaaccaa
     3781 cagatgaatc atttgcaggc gattataacc tccacagcta cacaaagtct atattatcgg
     3841 gttgcacgag cagtggaggc agcaacgcca ttgatgaggg gttgagactg gttttgcagg
     3901 tggggataaa gtgttctgaa gaatatccga gggataggat gagaacggat gaagcagtac
     3961 gagaattaat ctcaataaga tctaagttct tcagttccaa gacgactatt acagagagtc
     4021 ctcgagatgc tccgcaaagt tctcctcagg aatggatgtt aaatacggac atgcatacta
     4081 tgaagggtgg gcgcgccgac ccagctttct tgtacaaagt ggtgctcgag atggtagatc
     4141 tgactagtaa aggagaagaa cttttcactg gagttgtccc aattcttgtt gaattagatg
     4201 gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat gcaacatacg
     4261 gaaaacttac ccttaaattt atttgcacta ctggaaaact acctgttccg tggccaacac
     4321 ttgtcactac tttctcttat ggtgttcaat gcttttcaag atacccagat catatgaagc
     4381 ggcacgactt cttcaagagc gccatgcctg agggatacgt gcaggagagg accatcttct
     4441 tcaaggacga cgggaactac aagacacgtg ctgaagtcaa gtttgaggga gacaccctcg
     4501 tcaacaggat cgagcttaag ggaatcgatt tcaaggagga cggaaacatc ctcggccaca
     4561 agttggaata caactacaac tcccacaacg tatacatcat ggccgacaag caaaagaacg
     4621 gcatcaaagc caacttcaag acccgccaca acatcgaaga cggcggcgtg caactcgctg
     4681 atcattatca acaaaatact ccaattggcg atggccctgt ccttttacca gacaaccatt
     4741 acctgtccac acaatctgcc ctttcgaaag atcccaacga aaagagagac cacatggtcc
     4801 ttcttgagtt tgtaacagct gctgggatta cacatggcat ggatgaacta tacaaagcta
     4861 gccaccacca ccaccaccac gtgtgaccta ggtgagtcta gagagttaat taagacccgg
     4921 gactagtccc tagagtcctg ctttaatgag atatgcgaga cgcctatgat cgcatgatat
     4981 ttgctttcaa ttctgttgtg cacgttgtaa aaaacctgag catgtgtagc tcagatcctt
     5041 accgccggtt tcggttcatt ctaatgaata tatcacccgt tactatcgta tttttatgaa
     5101 taatattctc cgttcaattt actgattgta ccctactact tatatgtaca atattaaaat
     5161 gaaaacaata tattgtgctg aataggttta tagcgacatc tatgatagag cgccacaata
     5221 acaaacaatt gcgttttatt attacaaatc caattttaaa aaaagcggca gaaccggtca
     5281 aacctaaaag actgattaca taaatcttat tcaaatttca aaagtgcccc aggggctagt
     5341 atctacgaca caccgagcgg cgaactaata acgctcactg aagggaactc cggttccccg
     5401 ccggcgcgca tgggtgagat tccttgaagt tgagtattgg ccgtccgctc taccgaaagt
     5461 tacgggcacc attcaacccg gtccagcacg gcggccgggt aaccgacttg ctgccccgag
     5521 aattatgcag catttttttg gtgtatgtgg gccccaaatg aagtgcaggt caaaccttga
     5581 cagtgacgac aaatcgttgg gcgggtccag ggcgaatttt gcgacaacat gtcgaggctc
     5641 agcaggacct gcaggcatgc aagcttggca ctggccgtcg ttttacaacg tcgtgactgg
     5701 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     5761 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     5821 gaatgctaga gcagcttgag cttggatcag attgtcgttt cccgccttca gtttaaacta
     5881 tcagtgtttg acaggatata ttggcgggta aacctaagag aaaagagcgt ttattagaat
     5941 aatcggatat ttaaaagggc gtgaaaaggt ttatccgttc gtccatttgt atgtgcatgc
     6001 caaccacagg gttcccctcg ggatcaaagt actttgatcc aacccctccg ctgctatagt
     6061 gcagtcggct tctgacgttc agtgcagccg tcttctgaaa acgacatgtc gcacaagtcc
     6121 taagttacgc gacaggctgc cgccctgccc ttttcctggc gttttcttgt cgcgtgtttt
     6181 agtcgcataa agtagaatac ttgcgactag aaccggagac attacgccat gaacaagagc
     6241 gccgccgctg gcctgctggg ctatgcccgc gtcagcaccg acgaccagga cttgaccaac
     6301 caacgggccg aactgcacgc ggccggctgc accaagctgt tttccgagaa gatcaccggc
     6361 accaggcgcg accgcccgga gctggccagg atgcttgacc acctacgccc tggcgacgtt
     6421 gtgacagtga ccaggctaga ccgcctggcc cgcagcaccc gcgacctact ggacattgcc
     6481 gagcgcatcc aggaggccgg cgcgggcctg cgtagcctgg cagagccgtg ggccgacacc
     6541 accacgccgg ccggccgcat ggtgttgacc gtgttcgccg gcattgccga gttcgagcgt
     6601 tccctaatca tcgaccgcac ccggagcggg cgcgaggccg ccaaggcccg aggcgtgaag
     6661 tttggccccc gccctaccct caccccggca cagatcgcgc acgcccgcga gctgatcgac
     6721 caggaaggcc gcaccgtgaa agaggcggct gcactgcttg gcgtgcatcg ctcgaccctg
     6781 taccgcgcac ttgagcgcag cgaggaagtg acgcccaccg aggccaggcg gcgcggtgcc
     6841 ttccgtgagg acgcattgac cgaggccgac gccctggcgg ccgccgagaa tgaacgccaa
     6901 gaggaacaag catgaaaccg caccaggacg gccaggacga accgtttttc attaccgaag
     6961 agatcgaggc ggagatgatc gcggccgggt acgtgttcga gccgcccgcg cacgtctcaa
     7021 ccgtgcggct gcatgaaatc ctggccggtt tgtctgatgc caagctggcg gcctggccgg
     7081 ccagcttggc cgctgaagaa accgagcgcc gccgtctaaa aaggtgatgt gtatttgagt
     7141 aaaacagctt gcgtcatgcg gtcgctgcgt atatgatgcg atgagtaaat aaacaaatac
     7201 gcaaggggaa cgcatgaagg ttatcgctgt acttaaccag aaaggcgggt caggcaagac
     7261 gaccatcgca acccatctag cccgcgccct gcaactcgcc ggggccgatg ttctgttagt
     7321 cgattccgat ccccagggca gtgcccgcga ttgggcggcc gtgcgggaag atcaaccgct
     7381 aaccgttgtc ggcatcgacc gcccgacgat tgaccgcgac gtgaaggcca tcggccggcg
     7441 cgacttcgta gtgatcgacg gagcgcccca ggcggcggac ttggctgtgt ccgcgatcaa
     7501 ggcagccgac ttcgtgctga ttccggtgca gccaagccct tacgacatat gggccaccgc
     7561 cgacctggtg gagctggtta agcagcgcat tgaggtcacg gatggaaggc tacaagcggc
     7621 ctttgtcgtg tcgcgggcga tcaaaggcac gcgcatcggc ggtgaggttg ccgaggcgct
     7681 ggccgggtac gagctgccca ttcttgagtc ccgtatcacg cagcgcgtga gctacccagg
     7741 cactgccgcc gccggcacaa ccgttcttga atcagaaccc gagggcgacg ctgcccgcga
     7801 ggtccaggcg ctggccgctg aaattaaatc aaaactcatt tgagttaatg aggtaaagag
     7861 aaaatgagca aaagcacaaa cacgctaagt gccggccgtc cgagcgcacg cagcagcaag
     7921 gctgcaacgt tggccagcct ggcagacacg ccagccatga agcgggtcaa ctttcagttg
     7981 ccggcggagg atcacaccaa gctgaagatg tacgcggtac gccaaggcaa gaccattacc
     8041 gagctgctat ctgaatacat cgcgcagcta ccagagtaaa tgagcaaatg aataaatgag
     8101 tagatgaatt ttagcggcta aaggaggcgg catggaaaat caagaacaac caggcaccga
     8161 cgccgtggaa tgccccatgt gtggaggaac gggcggttgg ccaggcgtaa gcggctgggt
     8221 tgcctgccgg ccctgcaatg gcactggaac ccccaagccc gaggaatcgg cgtgagcggt
     8281 cgcaaaccat ccggcccggt acaaatcggc gcggcgctgg gtgatgacct ggtggagaag
     8341 ttgaaggccg cgcaggccgc ccagcggcaa cgcatcgagg cagaagcacg ccccggtgaa
     8401 tcgtggcaag cggccgctga tcgaatccgc aaagaatccc ggcaaccgcc ggcagccggt
     8461 gcgccgtcga ttaggaagcc gcccaagggc gacgagcaac cagatttttt cgttccgatg
     8521 ctctatgacg tgggcacccg cgatagtcgc agcatcatgg acgtggccgt tttccgtctg
     8581 tcgaagcgtg accgacgagc tggcgaggtg atccgctacg agcttccaga cgggcacgta
     8641 gaggtttccg cagggccggc cggcatggcc agtgtgtggg attacgacct ggtactgatg
     8701 gcggtttccc atctaaccga atccatgaac cgataccggg aagggaaggg agacaagccc
     8761 ggccgcgtgt tccgtccaca cgttgcggac gtactcaagt tctgccggcg agccgatggc
     8821 ggaaagcaga aagacgacct ggtagaaacc tgcattcggt taaacaccac gcacgttgcc
     8881 atgcagcgta cgaagaaggc caagaacggc cgcctggtga cggtatccga gggtgaagcc
     8941 ttgattagcc gctacaagat cgtaaagagc gaaaccgggc ggccggagta catcgagatc
     9001 gagctagctg attggatgta ccgcgagatc acagaaggca agaacccgga cgtgctgacg
     9061 gttcaccccg attacttttt gatcgatccc ggcatcggcc gttttctcta ccgcctggca
     9121 cgccgcgccg caggcaaggc agaagccaga tggttgttca agacgatcta cgaacgcagt
     9181 ggcagcgccg gagagttcaa gaagttctgt ttcaccgtgc gcaagctgat cgggtcaaat
     9241 gacctgccgg agtacgattt gaaggaggag gcggggcagg ctggcccgat cctagtcatg
     9301 cgctaccgca acctgatcga gggcgaagca tccgccggtt cctaatgtac ggagcagatg
     9361 ctagggcaaa ttgccctagc aggggaaaaa ggtcgaaaag gtctctttcc tgtggatagc
     9421 acgtacattg ggaacccaaa gccgtacatt gggaaccgga acccgtacat tgggaaccca
     9481 aagccgtaca ttgggaaccg gtcacacatg taagtgactg atataaaaga gaaaaaaggc
     9541 gatttttccg cctaaaactc tttaaaactt attaaaactc ttaaaacccg cctggcctgt
     9601 gcataactgt ctggccagcg cacagccgaa gagctgcaaa aagcgcctac ccttcggtcg
     9661 ctgcgctccc tacgccccgc cgcttcgcgt cggcctatcg cggccgctgg ccgctcaaaa
     9721 atggctggcc tacggccagg caatctacca gggcgcggac aagccgcgcc gtcgccactc
     9781 gaccgccggc gcccacatca aggcaccctg cctcgcgcgt ttcggtgatg acggtgaaaa
     9841 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
     9901 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac
     9961 ccagtcacgt agcgatagcg gagtgtatac tggcttaact atgcggcatc agagcagatt
    10021 gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
    10081 cgcatcaggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
    10141 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
    10201 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
    10261 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
    10321 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
    10381 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
    10441 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
    10501 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
    10561 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
    10621 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
    10681 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
    10741 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
    10801 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
    10861 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
    10921 taagggattt tggtcatgca ttctaggtac taaaacaatt catccagtaa aatataatat
    10981 tttattttct cccaatcagg cttgatcccc agtaagtcaa aaaatagctc gacatactgt
    11041 tcttccccga tatcctccct gatcgaccgg acgcagaagg caatgtcata ccacttgtcc
    11101 gccctgccgc ttctcccaag atcaataaag ccacttactt tgccatcttt cacaaagatg
    11161 ttgctgtctc ccaggtcgcc gtgggaaaag acaagttcct cttcgggctt ttccgtcttt
    11221 aaaaaatcat acagctcgcg cggatcttta aatggagtgt cttcttccca gttttcgcaa
    11281 tccacatcgg ccagatcgtt attcagtaag taatccaatt cggctaagcg gctgtctaag
    11341 ctattcgtat agggacaatc cgatatgtcg atggagtgaa agagcctgat gcactccgca
    11401 tacagctcga taatcttttc agggctttgt tcatcttcat actcttccga gcaaaggacg
    11461 ccatcggcct cactcatgag cagattgctc cagccatcat gccgttcaaa gtgcaggacc
    11521 tttggaacag gcagctttcc ttccagccat agcatcatgt ccttttcccg ttccacatca
    11581 taggtggtcc ctttataccg gctgtccgtc atttttaaat ataggttttc attttctccc
    11641 accagcttat ataccttagc aggagacatt ccttccgtat cttttacgca gcggtatttt
    11701 tcgatcagtt ttttcaattc cggtgatatt ctcattttag ccatttatta tttccttcct
    11761 cttttctaca gtatttaaag ataccccaag aagctaatta taacaagacg aactccaatt
    11821 cactgttcct tgcattctaa aaccttaaat accagaaaac agctttttca aagttgtttt
    11881 caaagttggc gtataacata gtatcgacgg agccgatttt gaaaccgcgg tgatcacagg
    11941 cagcaacgct ctgtcatcgt tacaatcaac atgctaccct ccgcgagatc atccgtgttt
    12001 caaacccggc agcttagttg ccgttcttcc gaatagcatc ggtaacatga gcaaagtctg
    12061 ccgccttaca acggctctcc cgctgacgcc gtcccggact gatgggctgc ctgtatcgag
    12121 tggtgatttt gtgccgagct gccggtcggg gagctgttgg ctggctggtg gcaggatata
    12181 ttgtggtgta aacaaattga cgcttagaca acttaataac acattgcgga cgtttttaat
    12241 gtactgaatt aacgccgaat taattcgagc tcggatctga taatttattt gaaaattcat
    12301 aagaaaagca aacgttacat gaattgatga aacaatacaa agacagataa agccacgcac
    12361 atttaggata ttggccgaga ttactgaata ttgagtaaga tcacggaatt tctgacagga
    12421 gcatgtcttc aattcagccc aaatggcagt tgaaatactc aaaccgcccc atatgcagga
    12481 gcggatcatt cattgtttgt ttggttgcct ttgccaacat gggagtccaa gattctgcag
    12541 tcaaatctcg gtgacgggca ggaccggacg gggcggtacc ggcaggctga agtccagctg
    12601 ccagaaaccc acgtcatgcc agttcccgtg cttgaagccg gccgcccgca gcatgccgcg
    12661 gggggcatat ccgagcgcct cgtgcatgcg cacgctcggg tcgttgggca gcccgatgac
    12721 agcgaccacg ctcttgaagc cctgtgcctc cagggacttc agcaggtggg tgtagagcgt
    12781 ggagcccagt cccgtccgct ggtggcgggg ggagacgtac acggtcgact cggccgtcca
    12841 gtcgtaggcg ttgcgtgcct tccaggggcc cgcgtaggcg atgccggcga cctcgccgtc
    12901 cacctcggcg acgagccagg gatagcgctc ccgcagacgg acgaggtcgt ccgtccactc
    12961 ctgcggttcc tgcggctcgg tacggaagtt gaccgtgctt gtctcgatgt agtggttgac
    13021 gatggtgcag accgccggca tgtccgcctc ggtggcacgg cggatgtcgg ccgggcgtcg
    13081 ttctgggctc atcgattcga tttggtgtat cgagattggt tatgaaattc agatgctagt
    13141 gtaatgtatt ggtaatttgg gaagatataa taggaagcaa ggctatttat ccatttctga
    13201 aaaggcgaaa tggcgtcacc gcgagcgtca cgcgcattcc gttcttgctg taaagcgttg
    13261 tttggtacac ttttgactag cgaggcttgg cgtgtcagcg tatctattca aaagtcgtta
    13321 atggctgcgg atcaagaaaa agttggaata gaaacagaat acccgcgaaa ttcaggcccg
    13381 gttgccatgt cctacacgcc gaaataaacg accaaattag tagaaaaata aaaactgact
    13441 cggatactta cgtcacgtct tgcgcactga tttgaaaaat ctcagaattc caatcccaca
    13501 aaaatctgag cttaacagca cagttgctcc tctcagagca gaatcgggta ttcaacaccc
    13561 tcatatcaac tactacgttg tgtataacgg tccacatgcc ggtatatacg atgactgggg
    13621 ttgtacaaag gcggcaacaa acggcgttcc cggagttgca cacaagaaat ttgccactat
    13681 tacagaggca agagcagcag ctgacgcgta cacaacaagt cagcaaacag acaggttgaa
    13741 cttcatcccc aaaggagaag ctcaactcaa gcccaagagc tttgctaagg ccctaacaag
    13801 cccaccaaag caaaaagccc actggctcac gctaggaacc aaaaggccca gcagtgatcc
    13861 agccccaaaa gagatctcct ttgccc
//
