LOCUS       sequence-197801-       13886 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  fluorescent tagging. Plant expression of EFR Y875F (15).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13886)
  TITLE     Robatzek Lab plasmids
REFERENCE   2  (bases 1 to 13886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: EFR Y875F (15)
FEATURES             Location/Qualifiers
     source          1..13886
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        72..417
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     360..380
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    429..453
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3583..3607)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3630..4340
                     /codon_start=1
                     /gene="mgfp5"
                     /product="GFP with folding enhancement mutations"
                     /label=mgfp5
                     /note="suitable for expression in plants due to removal of 
                     a cryptic intron"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKAN
                     FKTRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(3657..3685)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     4280..4300
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             4347..4364
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      4422..5129
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     primer_bind     complement(5154..5171)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5154..5170)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5163..5185)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    5373..5397
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6698..7327
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             7756..8829
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      8895..9089
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     misc_feature    9433..9573
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     9488..9507
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(9588..9605)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9759..10347)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9839..9858)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(10434..11228)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    11653..11677
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(11763..12015)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     CDS             complement(12025..12576)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(12582..12962)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
ORIGIN
        1 atggtggagc acgacactct ggtctactcc aaaaatgtca aagatacagt ctcagaagac
       61 caaagggcta ttgagacttt tcaacaaagg ataatttcgg gaaacctcct cggattccat
      121 tgcccagcta tctgtcactt catcgaaagg acagtagaaa aggaaggtgg ctcctacaaa
      181 tgccatcatt gcgataaagg aaaggctatc attcaagatc tctctgccga cagtggtccc
      241 aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct
      301 tcaaagcaag tggattgatg tgacatctcc actgacgtaa gggatgacgc acaatcccac
      361 tatccttcgc aagacccttc ctctatataa ggaagttcat ttcatttgga gaggacacgc
      421 tcgagatcac aagtttgtac aaaaaagcag gctccgcggc cgcccccttc accatgaagc
      481 tgtccttttc acttgttttc aatgctctca cgttgcttct tcaagtttgc atctttgctc
      541 aagccaggtt ttctaatgag actgatatgc aagctttgct tgagttcaag tctcaagttt
      601 ctgaaaacaa caagagagag gtcttggctt catggaatca ctcctcccca ttttgtaatt
      661 ggattggagt cacatgtggc cgcaggagag aaagagttat aagtttgaac cttggaggat
      721 tcaagttaac cggtgtgatc tcaccctcca ttggtaatct ctcctttctt agattactta
      781 atcttgcaga caactctttt ggaagtacca tccctcaaaa ggtgggaagg ctatttaggc
      841 ttcagtactt gaacatgagc tataatcttc tcgaaggaag gattccgtct agtctttcta
      901 actgctctag actgtcgacc gttgatttat cgtcaaacca tcttggacat ggtgttcctt
      961 cagaactagg ttcactttct aagcttgcca ttctggatct tagcaaaaac aaccttactg
     1021 gaaattttcc tgcatcttta ggaaacttga cgtcacttca gaagcttgac tttgcatata
     1081 accagatgag aggtgagatt ccagacgaag tagctagatt gactcaaatg gtgtttttcc
     1141 aaatagcact gaatagtttt tcaggtggtt ttcctcctgc attgtacaac atctcctctc
     1201 ttgagtctct atctctagct gacaatagct tttcgggtaa tcttagggct gattttggtt
     1261 atcttctacc aaatctaaga agacttcttt tgggaacaaa tcagttcact ggagctattc
     1321 ccaaaacact tgccaatatc tcaagccttg aaaggtttga tatctcatct aattacctgt
     1381 ctggtagtat ccctttgagc tttggaaagt tacgtaatct gtggtggtta gggattcgta
     1441 ataactctct tggaaataac tcgtccagtg gtcttgaatt tattggggct gtggcgaact
     1501 gcactcaatt agagtactta gatgttggtt acaatagact tggaggtgag cttcctgctt
     1561 ctatagccaa tctgtccact acattgacta gtctgttcct tggacaaaat cttatctctg
     1621 gaaccattcc tcatgacatc gggaatcttg taagcctgca agaactcagc ttagaaacaa
     1681 atatgttgag tggagaactt cccgtctctt tcgggaagct tttgaacttg caggttgtgg
     1741 atctgtattc aaatgcaata tcgggggaaa taccatctta ttttggcaac atgactcggt
     1801 tgcagaagct ccatttgaat agcaatagtt tccacggaag aatccctcag agtcttggac
     1861 gttgtcgata cttgctagac ctgtggatgg atacaaatag gttgaatggg actatacctc
     1921 aggaaatact gcaaattcca tccctcgctt acatagattt gtcaaacaat ttcttgacag
     1981 gccattttcc agaagaagtt ggaaagttag aacttcttgt tggactaggt gcttcgtaca
     2041 acaaattatc aggaaagatg ccacaagcta tagggggttg tctttcgatg gaatttctct
     2101 ttatgcaagg aaattcgttt gacggagcca ttccagatat aagccggttg gtaagcctaa
     2161 agaatgttga cttctccaac aacaatctct ctggccggat acctcgatat ctggccagtc
     2221 ttccttcgct gcgaaatctg aatctttcta tgaacaagtt tgagggaagg gtgccaacaa
     2281 caggagtgtt tcgaaatgct acagcagttt ctgtttttgg taacacaaat atttgcggag
     2341 gcgtccgaga aatgcaacta aagccatgca ttgtacaggc atctccaagg aagagaaagc
     2401 ctctgtcagt tagaaagaaa gttgtcagtg gtatttgtat aggtatagct tcgcttttgt
     2461 taatcataat tgtggcttct ctgtgttggt tcatgaagag gaaaaagaaa aacaatgcca
     2521 gtgatggtaa cccatctgat tctactactt tggggatgtt ccatgagaag gtaagttatg
     2581 aagagcttca tagtgcaaca agtcgcttct cttcaaccaa tttgattggt tcaggcaatt
     2641 tcggtaatgt gtttaaagga ttgcttggcc ctgagaataa actcgtcgcg gttaaagttt
     2701 tgaacctcct aaagcatgga gcgacgaaaa gctttatggc ggaatgtgaa accttcaagg
     2761 gtatacgaca tcgtaacctt gtaaaactga taacggtttg ttcaagcctt gattccgagg
     2821 gaaatgattt cagagctctg gtctatgagt tcatgccaaa aggaagtctg gatatgtggc
     2881 tgcagctaga agatctggaa agggtaaacg atcactcgag atctttaaca cccgcagaga
     2941 aactcaacat agcaatagat gtggcttcag ctttggagta tctgcacgtt cattgtcatg
     3001 accctgtagc tcactgtgat attaagccaa gcaacattct tctagacgat gatctgactg
     3061 ctcatgttag tgactttggt ttggctcagc tcctctttaa atacgatcga gaatcctttc
     3121 taaaccagtt tagttctgct ggtgtcagag gcaccattgg ctatgccgcg ccagagtatg
     3181 gaatgggagg ccaaccatca atacaaggag atgtgtacag cttcggaatt ctacttttgg
     3241 agatgtttag tggaaagaaa ccaacagatg aatcatttgc aggcgattat aacctccaca
     3301 gctacacaaa gtctatatta tcgggttgca cgagcagtgg aggcagcaac gccattgatg
     3361 aggggttgag actggttttg caggtgggga taaagtgttc tgaagaatat ccgagggata
     3421 ggatgagaac ggatgaagca gtacgagaat taatctcaat aagatctaag ttcttcagtt
     3481 ccaagacgac tattacagag agtcctcgag atgctccgca aagttctcct caggaatgga
     3541 tgttaaatac ggacatgcat actatgaagg gtgggcgcgc cgacccagct ttcttgtaca
     3601 aagtggtgct cgagatggta gatctgacta gtaaaggaga agaacttttc actggagttg
     3661 tcccaattct tgttgaatta gatggtgatg ttaatgggca caaattttct gtcagtggag
     3721 agggtgaagg tgatgcaaca tacggaaaac ttacccttaa atttatttgc actactggaa
     3781 aactacctgt tccgtggcca acacttgtca ctactttctc ttatggtgtt caatgctttt
     3841 caagataccc agatcatatg aagcggcacg acttcttcaa gagcgccatg cctgagggat
     3901 acgtgcagga gaggaccatc ttcttcaagg acgacgggaa ctacaagaca cgtgctgaag
     3961 tcaagtttga gggagacacc ctcgtcaaca ggatcgagct taagggaatc gatttcaagg
     4021 aggacggaaa catcctcggc cacaagttgg aatacaacta caactcccac aacgtataca
     4081 tcatggccga caagcaaaag aacggcatca aagccaactt caagacccgc cacaacatcg
     4141 aagacggcgg cgtgcaactc gctgatcatt atcaacaaaa tactccaatt ggcgatggcc
     4201 ctgtcctttt accagacaac cattacctgt ccacacaatc tgccctttcg aaagatccca
     4261 acgaaaagag agaccacatg gtccttcttg agtttgtaac agctgctggg attacacatg
     4321 gcatggatga actatacaaa gctagccacc accaccacca ccacgtgtga cctaggtgag
     4381 tctagagagt taattaagac ccgggactag tccctagagt cctgctttaa tgagatatgc
     4441 gagacgccta tgatcgcatg atatttgctt tcaattctgt tgtgcacgtt gtaaaaaacc
     4501 tgagcatgtg tagctcagat ccttaccgcc ggtttcggtt cattctaatg aatatatcac
     4561 ccgttactat cgtattttta tgaataatat tctccgttca atttactgat tgtaccctac
     4621 tacttatatg tacaatatta aaatgaaaac aatatattgt gctgaatagg tttatagcga
     4681 catctatgat agagcgccac aataacaaac aattgcgttt tattattaca aatccaattt
     4741 taaaaaaagc ggcagaaccg gtcaaaccta aaagactgat tacataaatc ttattcaaat
     4801 ttcaaaagtg ccccaggggc tagtatctac gacacaccga gcggcgaact aataacgctc
     4861 actgaaggga actccggttc cccgccggcg cgcatgggtg agattccttg aagttgagta
     4921 ttggccgtcc gctctaccga aagttacggg caccattcaa cccggtccag cacggcggcc
     4981 gggtaaccga cttgctgccc cgagaattat gcagcatttt tttggtgtat gtgggcccca
     5041 aatgaagtgc aggtcaaacc ttgacagtga cgacaaatcg ttgggcgggt ccagggcgaa
     5101 ttttgcgaca acatgtcgag gctcagcagg acctgcaggc atgcaagctt ggcactggcc
     5161 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     5221 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     5281 caacagttgc gcagcctgaa tggcgaatgc tagagcagct tgagcttgga tcagattgtc
     5341 gtttcccgcc ttcagtttaa actatcagtg tttgacagga tatattggcg ggtaaaccta
     5401 agagaaaaga gcgtttatta gaataatcgg atatttaaaa gggcgtgaaa aggtttatcc
     5461 gttcgtccat ttgtatgtgc atgccaacca cagggttccc ctcgggatca aagtactttg
     5521 atccaacccc tccgctgcta tagtgcagtc ggcttctgac gttcagtgca gccgtcttct
     5581 gaaaacgaca tgtcgcacaa gtcctaagtt acgcgacagg ctgccgccct gcccttttcc
     5641 tggcgttttc ttgtcgcgtg ttttagtcgc ataaagtaga atacttgcga ctagaaccgg
     5701 agacattacg ccatgaacaa gagcgccgcc gctggcctgc tgggctatgc ccgcgtcagc
     5761 accgacgacc aggacttgac caaccaacgg gccgaactgc acgcggccgg ctgcaccaag
     5821 ctgttttccg agaagatcac cggcaccagg cgcgaccgcc cggagctggc caggatgctt
     5881 gaccacctac gccctggcga cgttgtgaca gtgaccaggc tagaccgcct ggcccgcagc
     5941 acccgcgacc tactggacat tgccgagcgc atccaggagg ccggcgcggg cctgcgtagc
     6001 ctggcagagc cgtgggccga caccaccacg ccggccggcc gcatggtgtt gaccgtgttc
     6061 gccggcattg ccgagttcga gcgttcccta atcatcgacc gcacccggag cgggcgcgag
     6121 gccgccaagg cccgaggcgt gaagtttggc ccccgcccta ccctcacccc ggcacagatc
     6181 gcgcacgccc gcgagctgat cgaccaggaa ggccgcaccg tgaaagaggc ggctgcactg
     6241 cttggcgtgc atcgctcgac cctgtaccgc gcacttgagc gcagcgagga agtgacgccc
     6301 accgaggcca ggcggcgcgg tgccttccgt gaggacgcat tgaccgaggc cgacgccctg
     6361 gcggccgccg agaatgaacg ccaagaggaa caagcatgaa accgcaccag gacggccagg
     6421 acgaaccgtt tttcattacc gaagagatcg aggcggagat gatcgcggcc gggtacgtgt
     6481 tcgagccgcc cgcgcacgtc tcaaccgtgc ggctgcatga aatcctggcc ggtttgtctg
     6541 atgccaagct ggcggcctgg ccggccagct tggccgctga agaaaccgag cgccgccgtc
     6601 taaaaaggtg atgtgtattt gagtaaaaca gcttgcgtca tgcggtcgct gcgtatatga
     6661 tgcgatgagt aaataaacaa atacgcaagg ggaacgcatg aaggttatcg ctgtacttaa
     6721 ccagaaaggc gggtcaggca agacgaccat cgcaacccat ctagcccgcg ccctgcaact
     6781 cgccggggcc gatgttctgt tagtcgattc cgatccccag ggcagtgccc gcgattgggc
     6841 ggccgtgcgg gaagatcaac cgctaaccgt tgtcggcatc gaccgcccga cgattgaccg
     6901 cgacgtgaag gccatcggcc ggcgcgactt cgtagtgatc gacggagcgc cccaggcggc
     6961 ggacttggct gtgtccgcga tcaaggcagc cgacttcgtg ctgattccgg tgcagccaag
     7021 cccttacgac atatgggcca ccgccgacct ggtggagctg gttaagcagc gcattgaggt
     7081 cacggatgga aggctacaag cggcctttgt cgtgtcgcgg gcgatcaaag gcacgcgcat
     7141 cggcggtgag gttgccgagg cgctggccgg gtacgagctg cccattcttg agtcccgtat
     7201 cacgcagcgc gtgagctacc caggcactgc cgccgccggc acaaccgttc ttgaatcaga
     7261 acccgagggc gacgctgccc gcgaggtcca ggcgctggcc gctgaaatta aatcaaaact
     7321 catttgagtt aatgaggtaa agagaaaatg agcaaaagca caaacacgct aagtgccggc
     7381 cgtccgagcg cacgcagcag caaggctgca acgttggcca gcctggcaga cacgccagcc
     7441 atgaagcggg tcaactttca gttgccggcg gaggatcaca ccaagctgaa gatgtacgcg
     7501 gtacgccaag gcaagaccat taccgagctg ctatctgaat acatcgcgca gctaccagag
     7561 taaatgagca aatgaataaa tgagtagatg aattttagcg gctaaaggag gcggcatgga
     7621 aaatcaagaa caaccaggca ccgacgccgt ggaatgcccc atgtgtggag gaacgggcgg
     7681 ttggccaggc gtaagcggct gggttgcctg ccggccctgc aatggcactg gaacccccaa
     7741 gcccgaggaa tcggcgtgag cggtcgcaaa ccatccggcc cggtacaaat cggcgcggcg
     7801 ctgggtgatg acctggtgga gaagttgaag gccgcgcagg ccgcccagcg gcaacgcatc
     7861 gaggcagaag cacgccccgg tgaatcgtgg caagcggccg ctgatcgaat ccgcaaagaa
     7921 tcccggcaac cgccggcagc cggtgcgccg tcgattagga agccgcccaa gggcgacgag
     7981 caaccagatt ttttcgttcc gatgctctat gacgtgggca cccgcgatag tcgcagcatc
     8041 atggacgtgg ccgttttccg tctgtcgaag cgtgaccgac gagctggcga ggtgatccgc
     8101 tacgagcttc cagacgggca cgtagaggtt tccgcagggc cggccggcat ggccagtgtg
     8161 tgggattacg acctggtact gatggcggtt tcccatctaa ccgaatccat gaaccgatac
     8221 cgggaaggga agggagacaa gcccggccgc gtgttccgtc cacacgttgc ggacgtactc
     8281 aagttctgcc ggcgagccga tggcggaaag cagaaagacg acctggtaga aacctgcatt
     8341 cggttaaaca ccacgcacgt tgccatgcag cgtacgaaga aggccaagaa cggccgcctg
     8401 gtgacggtat ccgagggtga agccttgatt agccgctaca agatcgtaaa gagcgaaacc
     8461 gggcggccgg agtacatcga gatcgagcta gctgattgga tgtaccgcga gatcacagaa
     8521 ggcaagaacc cggacgtgct gacggttcac cccgattact ttttgatcga tcccggcatc
     8581 ggccgttttc tctaccgcct ggcacgccgc gccgcaggca aggcagaagc cagatggttg
     8641 ttcaagacga tctacgaacg cagtggcagc gccggagagt tcaagaagtt ctgtttcacc
     8701 gtgcgcaagc tgatcgggtc aaatgacctg ccggagtacg atttgaagga ggaggcgggg
     8761 caggctggcc cgatcctagt catgcgctac cgcaacctga tcgagggcga agcatccgcc
     8821 ggttcctaat gtacggagca gatgctaggg caaattgccc tagcagggga aaaaggtcga
     8881 aaaggtctct ttcctgtgga tagcacgtac attgggaacc caaagccgta cattgggaac
     8941 cggaacccgt acattgggaa cccaaagccg tacattggga accggtcaca catgtaagtg
     9001 actgatataa aagagaaaaa aggcgatttt tccgcctaaa actctttaaa acttattaaa
     9061 actcttaaaa cccgcctggc ctgtgcataa ctgtctggcc agcgcacagc cgaagagctg
     9121 caaaaagcgc ctacccttcg gtcgctgcgc tccctacgcc ccgccgcttc gcgtcggcct
     9181 atcgcggccg ctggccgctc aaaaatggct ggcctacggc caggcaatct accagggcgc
     9241 ggacaagccg cgccgtcgcc actcgaccgc cggcgcccac atcaaggcac cctgcctcgc
     9301 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
     9361 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
     9421 cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt
     9481 aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg
     9541 cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac
     9601 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     9661 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     9721 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     9781 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     9841 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     9901 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     9961 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
    10021 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
    10081 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
    10141 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
    10201 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
    10261 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
    10321 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
    10381 gctcagtgga acgaaaactc acgttaaggg attttggtca tgcattctag gtactaaaac
    10441 aattcatcca gtaaaatata atattttatt ttctcccaat caggcttgat ccccagtaag
    10501 tcaaaaaata gctcgacata ctgttcttcc ccgatatcct ccctgatcga ccggacgcag
    10561 aaggcaatgt cataccactt gtccgccctg ccgcttctcc caagatcaat aaagccactt
    10621 actttgccat ctttcacaaa gatgttgctg tctcccaggt cgccgtggga aaagacaagt
    10681 tcctcttcgg gcttttccgt ctttaaaaaa tcatacagct cgcgcggatc tttaaatgga
    10741 gtgtcttctt cccagttttc gcaatccaca tcggccagat cgttattcag taagtaatcc
    10801 aattcggcta agcggctgtc taagctattc gtatagggac aatccgatat gtcgatggag
    10861 tgaaagagcc tgatgcactc cgcatacagc tcgataatct tttcagggct ttgttcatct
    10921 tcatactctt ccgagcaaag gacgccatcg gcctcactca tgagcagatt gctccagcca
    10981 tcatgccgtt caaagtgcag gacctttgga acaggcagct ttccttccag ccatagcatc
    11041 atgtcctttt cccgttccac atcataggtg gtccctttat accggctgtc cgtcattttt
    11101 aaatataggt tttcattttc tcccaccagc ttatatacct tagcaggaga cattccttcc
    11161 gtatctttta cgcagcggta tttttcgatc agttttttca attccggtga tattctcatt
    11221 ttagccattt attatttcct tcctcttttc tacagtattt aaagataccc caagaagcta
    11281 attataacaa gacgaactcc aattcactgt tccttgcatt ctaaaacctt aaataccaga
    11341 aaacagcttt ttcaaagttg ttttcaaagt tggcgtataa catagtatcg acggagccga
    11401 ttttgaaacc gcggtgatca caggcagcaa cgctctgtca tcgttacaat caacatgcta
    11461 ccctccgcga gatcatccgt gtttcaaacc cggcagctta gttgccgttc ttccgaatag
    11521 catcggtaac atgagcaaag tctgccgcct tacaacggct ctcccgctga cgccgtcccg
    11581 gactgatggg ctgcctgtat cgagtggtga ttttgtgccg agctgccggt cggggagctg
    11641 ttggctggct ggtggcagga tatattgtgg tgtaaacaaa ttgacgctta gacaacttaa
    11701 taacacattg cggacgtttt taatgtactg aattaacgcc gaattaattc gagctcggat
    11761 ctgataattt atttgaaaat tcataagaaa agcaaacgtt acatgaattg atgaaacaat
    11821 acaaagacag ataaagccac gcacatttag gatattggcc gagattactg aatattgagt
    11881 aagatcacgg aatttctgac aggagcatgt cttcaattca gcccaaatgg cagttgaaat
    11941 actcaaaccg ccccatatgc aggagcggat cattcattgt ttgtttggtt gcctttgcca
    12001 acatgggagt ccaagattct gcagtcaaat ctcggtgacg ggcaggaccg gacggggcgg
    12061 taccggcagg ctgaagtcca gctgccagaa acccacgtca tgccagttcc cgtgcttgaa
    12121 gccggccgcc cgcagcatgc cgcggggggc atatccgagc gcctcgtgca tgcgcacgct
    12181 cgggtcgttg ggcagcccga tgacagcgac cacgctcttg aagccctgtg cctccaggga
    12241 cttcagcagg tgggtgtaga gcgtggagcc cagtcccgtc cgctggtggc ggggggagac
    12301 gtacacggtc gactcggccg tccagtcgta ggcgttgcgt gccttccagg ggcccgcgta
    12361 ggcgatgccg gcgacctcgc cgtccacctc ggcgacgagc cagggatagc gctcccgcag
    12421 acggacgagg tcgtccgtcc actcctgcgg ttcctgcggc tcggtacgga agttgaccgt
    12481 gcttgtctcg atgtagtggt tgacgatggt gcagaccgcc ggcatgtccg cctcggtggc
    12541 acggcggatg tcggccgggc gtcgttctgg gctcatcgat tcgatttggt gtatcgagat
    12601 tggttatgaa attcagatgc tagtgtaatg tattggtaat ttgggaagat ataataggaa
    12661 gcaaggctat ttatccattt ctgaaaaggc gaaatggcgt caccgcgagc gtcacgcgca
    12721 ttccgttctt gctgtaaagc gttgtttggt acacttttga ctagcgaggc ttggcgtgtc
    12781 agcgtatcta ttcaaaagtc gttaatggct gcggatcaag aaaaagttgg aatagaaaca
    12841 gaatacccgc gaaattcagg cccggttgcc atgtcctaca cgccgaaata aacgaccaaa
    12901 ttagtagaaa aataaaaact gactcggata cttacgtcac gtcttgcgca ctgatttgaa
    12961 aaatctcaga attccaatcc cacaaaaatc tgagcttaac agcacagttg ctcctctcag
    13021 agcagaatcg ggtattcaac accctcatat caactactac gttgtgtata acggtccaca
    13081 tgccggtata tacgatgact ggggttgtac aaaggcggca acaaacggcg ttcccggagt
    13141 tgcacacaag aaatttgcca ctattacaga ggcaagagca gcagctgacg cgtacacaac
    13201 aagtcagcaa acagacaggt tgaacttcat ccccaaagga gaagctcaac tcaagcccaa
    13261 gagctttgct aaggccctaa caagcccacc aaagcaaaaa gcccactggc tcacgctagg
    13321 aaccaaaagg cccagcagtg atccagcccc aaaagagatc tcctttgccc cggagattac
    13381 aatggacgat ttcctctatc tttacgatct aggaaggaag ttcgaaggtg aaggtgacga
    13441 cactatgttc accactgata atgagaaggt tagcctcttc aatttcagaa agaatgctga
    13501 cccacagatg gttagagagg cctacgcagc aggtctcatc aagacgatct acccgagtaa
    13561 caatctccag gagatcaaat accttcccaa gaaggttaaa gatgcagtca aaagattcag
    13621 gactaattgc atcaagaaca cagagaaaga catatttctc aagatcagaa gtactattcc
    13681 agtatggacg attcaaggct tgcttcataa accaaggcaa gtaatagaga ttggagtctc
    13741 taaaaaggta gttcctactg aatctaaggc catgcatgga gtctaagatt caaatcgagg
    13801 atctaacaga actcgccgtg aagactggcg aacagttcat acagagtctt ttacgactca
    13861 atgacaagaa gaaaatcttc gtcaac
//
