LOCUS       sequence-197802-       10273 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Venus-tagged human Scribble protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10273)
  AUTHORS   Tatsis N, Lannigan DA, Macara IG
  TITLE     The function of the p190 Rho GTPase-activating protein is controlled
            by its N-terminal GTP binding domain.
  JOURNAL   J Biol Chem. 1998 Dec 18. 273(51):34631-8.
  PUBMED    9852136
REFERENCE   2  (bases 1 to 10273)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 1998 Dec 18. 273(51):34631-8."
COMMENT     Sequence Label: pKvenus-Scribble
FEATURES             Location/Qualifiers
     source          1..10273
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    102..123
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(138..143,144..161,162..168)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    176..192
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     181..203
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     200..216
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     200..216
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        245..624
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        625..828
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     779..799
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     825..849
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        1059..1077
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1059..1076
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     1090..1109
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             join(1167..1169,1170..1172,1173..1883)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1473..1492)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             3354..3365
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     regulatory      6804..6813
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    6817..6951
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6854..6873)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6908..6927
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        7020..7377
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7020..7211
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7043..7063)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7228..7363
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7397..7414)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7397..7413)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7406..7428)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7626..8081
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7713..7732)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7923..7944
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8098..8117)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8217..8239
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8277..8295)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8363..8467
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8468..8536,8537..9328)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8686..8705)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9499..10087
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9988..10007
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10241..10258
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
       61 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
      121 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
      181 agcggataac aatttcacac aggaaacagc tatgacatga ttacgaatta attcgagctc
      241 gcccgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
      301 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
      361 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
      421 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
      481 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
      541 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
      601 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
      661 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
      721 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
      781 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
      841 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgggac
      901 cgatccagcc tccgcggccg ggaacggtgc attggaacgc ggattccccg tgccaagagt
      961 gacgtaagta ccgcctatag agtctatagg cccaccccct tggcttcgtt agaacgcggc
     1021 tacaattaat acataacctt atgtatcata cacatacgat ttaggtgaca ctatagaata
     1081 acatccactt tgcctttctc tccacaggtg tccactccca ggtccaactg cacctcggtt
     1141 ctaagcttct gcaggtcgac tctagaatgg tgagcaaggg cgaggagctg ttcaccgggg
     1201 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
     1261 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagctgatc tgcaccaccg
     1321 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gggctacggc ctgcagtgct
     1381 tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
     1441 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
     1501 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
     1561 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
     1621 atatcaccgc cgacaagcag aagaacggca tcaaggccaa cttcaagatc cgccacaaca
     1681 tcgaggacgg cggcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
     1741 gccccgtgct gctgcccgac aaccactacc tgagctacca gtccgccctg agcaaagacc
     1801 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
     1861 tcggcatgga cgagctgtac aagtttggat ccatgctcaa gtgcatcccg ctgtggcgct
     1921 gcaaccggca cgtggagtcg gtggacaagc ggcactgttc gctgcaggcc gtgccggagg
     1981 agatctaccg ctacagccgc agcctggagg agctgctgct cgacgccaac cagctgcgcg
     2041 agctgcccaa gccttttttc cggctgctga acttgcgcaa gctgggcctg agcgacaacg
     2101 agatccagcg gttgcctccc gaggtggcca acttcatgca gctggtggag ctggacgtgt
     2161 cccggaacga tatccctgag atcccggaga gcatcaagtt ctgcaaggct ctggagatcg
     2221 cggacttcag cgggaacccc ctctccaggc tccctgatgg cttcactcag ctgcgcagcc
     2281 tggctcacct ggccctgaat gatgtgtctc tgcaggcact gcccggggac gtgggcaacc
     2341 tcgccaacct ggtgaccctg gagctccggg agaacctgct caagtccctg ccagcgtccc
     2401 tgtcatttct ggtcaagctg gaacagctgg atctgggagg caacgatctg gaagtgctgc
     2461 cagacactct ggggactctg cccaatcttc gggagctgtg gcttgaccgg aaccagctgt
     2521 cagcactgcc cccggagctc gggaacctgc ggcgcctggt gtgcctggac gtgtcggaaa
     2581 accggctgga ggagctgcct gctgagctcg gcgggctggt gctgctcact gacctgctgc
     2641 tgtcccagaa cctgctgcgg aggctgcccg acggcatcgg tcagctgaag cagctatcca
     2701 tcctaaaggt agaccagaat cggctgtgcg aggtgactga ggccatcggg gactgtgaga
     2761 acctctctga gctgatcctc acggagaacc tgctgatggc cctgccccgc tccctgggaa
     2821 agctgactaa gctgaccaac ctcaacgtgg accggaacca cctcgaggcg ctgccgcccg
     2881 agatcggggg ctgtgtggca ctcagcgtcc tctccttgag ggacaaccgc ctggccgtcc
     2941 tgccaccaga gctggcccac acgacagagc tgcacgtgct ggacgtggcg gggaaccgcc
     3001 tgcagagtct gccgttcgcg ctcacccacc tcaatctcaa ggccctgtgg ctggcagaga
     3061 accaggcgca gcccatgctc cggttccaga cggaggatga tgcccggacc ggcgagaagg
     3121 tgctcacctg ctacttgctg ccccagcagc ccccactcag cctcgaggat gctgggcagc
     3181 aggggagcct ctcggagacc tggagcgatg ccccgccgag ccgcgtcagc gtcatccagt
     3241 tcctggaggc ccccataggt gatgaggacg ctgaggaagc tgcagctgag aagcggggcc
     3301 tacagcgccg ggccacacct caccccagcg agctcaaggt gatgaagagg agcatcgagg
     3361 ggcggcggag cgaggcctgc ccttgccagc cagactctgg gtcgcccttg cctgcagagg
     3421 aggagaagcg gctgagtgtc gagtctggcc tgagtgaaga ctctcgccca tctgccagca
     3481 cagtctctga ggctgagccc gagggcccgt cggctgaggc acagggtggg agccagcagg
     3541 aagccacgac tgctggcggg gaggaagacg ccgaagagga ctaccaggag cccacggtgc
     3601 atttcgcaga ggacgcactg ctgcccgggg atgacaggga gatcgaggag gggcagcctg
     3661 aggccccctg gaccctgcca ggcgggaggc agcggctcat ccgcaaggac acacctcact
     3721 acaaaaagca cttcaagatc tccaagctgc cccagcccga ggccgttgtg gctctgctgc
     3781 agggcatgca gcctgatggg gagggccctg tggctcccgg gggctggcac aatggccccc
     3841 acgcaccctg ggctcctcgg gcccagaagg aggaggagga ggaggaagag ggtagtcctc
     3901 aggaggagga agaggaggag gaggaaaaca gggctgaaga ggaagaggcc agcactgagg
     3961 aggaggacaa ggagggggcc gtggtttctg cgccctctgt caagggagtg tcgtttgacc
     4021 aggccaataa cctgctgata gagcctgctc gcattgagga ggaagagctg accctcacca
     4081 tcctgcggca gactgggggc ctgggcatca gcattgcggg cggcaagggc tccacaccct
     4141 ataaggggga cgacgagggc atattcatct ctcgggtgtc cgaggaaggc cctgcggccc
     4201 gggctggagt ccgtgtgggt gacaagctcc tggaggtgaa tggtgtggct ctgcagggcg
     4261 ccgagcacca cgaggccgtg gaggcgctcc ggggggccgg cactgccgtg cagatgcgag
     4321 tgtggcggga gcgcatggtg gagcctgaga acgcggtcac caccacgccg ctgcggcccg
     4381 aggatgatta cagcccccga gagcggcggg gaggggggct gcgcctgccc ctgctcccgc
     4441 ctgagagccc cgggcccctc cgtcagcgcc acgtggcctg cctggcacgc agcgagaggg
     4501 ggctgggctt cagcattgct ggtgggaaag gctccacacc ctacagggct ggtgatgcgg
     4561 gcatcttcgt ctcccgcatt gccgagggcg gtgctgctca ccgcgcgggc acactgcagg
     4621 ttggcgaccg cgtcctctct attaatggag tggacgtgac tgaggccagg catgaccacg
     4681 ccgtctccct gctgaccgct gcctccccca ccatcgccct gctgttggag cgggaggctg
     4741 ggggccctct tcctcccagc cctctgccac attcctcacc ccccaccgct gctgttgcca
     4801 ccaccagcat aaccactgcc acccccgggg tgcctgggtt gccgagcctg gcccccagcc
     4861 tgctggctgc cgcgttggaa gggccatacc cagtggagga gatccgtctg ccaagagctg
     4921 ggggccctct ggggcttagt attgtcggag gctccgacca ttccagccac ccgtttggtg
     4981 tccaggagcc tggtgtgttc atctccaagg tgctcccgcg gggcctggcc gctcgcagcg
     5041 gcctgcgggt tggggaccgc atcctggcag tgaacgggca agacgtgcgg gatgccacgc
     5101 accaagaagc agtcagtgcc ctgctccggc cctgcctgga gctgtcgctg ctggtgcgga
     5161 gggacccggc acccccgggc ctacgggaac tgtgcatcca gaaggcacct ggggagaggc
     5221 tgggcatcag catccgcggg ggtgccaggg gccacgctgg caacccccgc gaccccacag
     5281 acgagggcat cttcatctcc aaggtgagcc ccacgggggc agccgggcgc gacggtcggc
     5341 tgcgtgtggg tttgcggctg ttggaggtga accagcagag cctgctgggc ctgacgcacg
     5401 gcgaggcggt gcagctgctc cgcagtgtgg gcgacaccct caccgtgctg gtctgtgacg
     5461 gcttcgaggc cagcaccgac gcagccctgg aggtgtcccc aggtgtcatt gccaacccct
     5521 ttgcggcagg catcggccac cggaacagcc tggagagcat ctcttccatc gaccgggagc
     5581 tgagccctga gggcccaggc aaggagaagg agctgcctgg acagaccctg cactgggggc
     5641 ccgaggccac agaagccgca ggtcggggtc tgcagcccct gaagctggac taccgcgccc
     5701 tggccgccgt gcccagcgct ggcagcgtgc agagggtacc gtctggagca gctggaggga
     5761 agatggctga atctccctgc tcccctagtg gccagcagcc gccctccccg ccttctccgg
     5821 atgagctgcc cgccaatgtg aagcaggcct acagggcctt cgcggccgtg cccacttctc
     5881 acccgcctga ggatgcccct gcccagcccc ccacgcctgg gcctgcagcc tccccggagc
     5941 agctgtcctt ccgggagcgg cagaagtact ttgagctgga ggtgcgcgtg ccccaggccg
     6001 agggcccccc taagcgcgtg tccctggtgg gtgctgacga cctgcggaag atgcaggagg
     6061 aggaagccag aaaactacag cagaagagag cgcagatgct gcgggaggcg gcagaggctg
     6121 gggccgaagc gaggctcgcc ctggacgggg agacgctggg cgaggaggaa caggaggatg
     6181 agcagccacc ctgggccagc ccgagcccca cctcaaggca gagcccggcg tcccccccgc
     6241 ccctgggagg tggcgccccg gtgcggacgg ccaaagctga acggcgccac caggagcggc
     6301 tgcgcgtgca gagtccggag ccaccggcac ccgagcgtgc cctgtcccct gccgagctcc
     6361 gggccctgga ggccgagaag cgtgcgctgt ggagggcagc caggatgaag tcattggaac
     6421 aggacgctct ccgagcacag atggtcctca gcaggtccca ggaaggccgg ggcacgcggg
     6481 ggcccctgga gcgactggcc gaggcccctt cccctgcgcc caccccgtcg cccacccctg
     6541 tggaagacct cggcccccag accagcacct ccccgggacg cctgtcaccg gactttgctg
     6601 aggagttgag gtccctggaa ccatctccca gccctggccc gcaggaggag gatggagaag
     6661 tggctctggt gcttctgggc aggccctcac ccggcgctgt gggccctgaa gatgtggcac
     6721 tgtgcagcag ccgccgcccc gtaaggcctg ggcgccgtgg cctgggccct gtgccctcct
     6781 agcggccgcg aattcaatcg atggccgcca tggcccaact tgtttattgc agcttataat
     6841 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     6901 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat cgggaattaa
     6961 ttcggcgcag caccatggcc tgaaataacc tctgaaagag gaacttggtt aggtaccttc
     7021 tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     7081 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     7141 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     7201 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     7261 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     7321 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     7381 ctgttaacag cttggcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     7441 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
     7501 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga
     7561 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatacgt caaagcaacc
     7621 atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     7681 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     7741 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     7801 atttagtgct ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag
     7861 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     7921 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcgggct attcttttga
     7981 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     8041 atttaacgcg aattttaaca aaatattaac gtttacaatt ttatggtgca ctctcagtac
     8101 aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc
     8161 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
     8221 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct
     8281 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
     8341 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     8401 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     8461 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     8521 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     8581 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     8641 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     8701 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     8761 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     8821 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     8881 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     8941 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     9001 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     9061 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     9121 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     9181 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     9241 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     9301 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     9361 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     9421 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     9481 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     9541 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     9601 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     9661 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     9721 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     9781 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     9841 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     9901 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     9961 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
    10021 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
    10081 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
    10141 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
    10201 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
    10261 agcggaagag cgc
//
