LOCUS       sequence-197804-        1994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses SLC39A14/ZIP14 wild-type in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Liu C, Hutchens S, Jursa T, Shawlot W, Polishchuk EV, Polishchuk RS,
            Dray BK, Gore AC, Aschner M, Smith DR, Mukhopadhyay S
  TITLE     Hypothyroidism induced by loss of the manganese efflux transporter 
            SLC30A10 may be explained by reduced thyroxine production.
  JOURNAL   J Biol Chem. 2017 Oct 6;292(40):16605-16615. doi: 
            10.1074/jbc.M117.804989. Epub 2017 Aug 31.
  PUBMED    28860195
REFERENCE   2  (bases 1 to 1994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M117.804989"; journalName: "J Biol Chem"; date: 
            "2017-10-6- 6"; volume: "292"; issue: "40"; pages: "16605-16615"
COMMENT     Sequence Label: SLC39A14-GFP
FEATURES             Location/Qualifiers
     source          1..1994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     1..21
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        6..50
                     /label=CMVd3 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields strongly reduced expression 
                     relative to the full-length promoter"
     regulatory      1618..1627
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(1669..1690)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1930..1949)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct ggtttagtga
       61 accgtcagat ccgctagcgc taccggactc agatctcgag ctcaagcttc gaattctgca
      121 gtcgacggta ccatgaagct gctgctgctg cacccggcct tccagagctg cctcctgctg
      181 accctgcttg gcttatggag aaccacccct gaggctcacg cttcatccct gggtgcacca
      241 gctatcagcg ctgcctcctt cctgcaggat ctaatacatc ggtatggcga gggtgacagc
      301 ctcactctgc agcagctgaa ggccctactc aaccacctgg atgtgggagt gggccggggt
      361 aatgtcaccc agcacgtgca aggacacagg aacctctcca cgtgctttag ttctggagac
      421 ctcttcactg cccacaattt cagcgagcag tcgcggattg ggagcagcga gctccaggag
      481 ttctgcccca ccatcctcca gcagctggat tcccgggcct gcacctcgga gaaccaggaa
      541 aacgaggaga atgagcagac ggaggagggg cggccaagcg ctgttgaagt gtggggatac
      601 ggtctcctct gtgtgaccgt catctccctc tgctccctcc tgggggccag cgtggtgccc
      661 ttcatgaaga agacctttta caagaggctg ctgctctact tcatagctct ggcgattgga
      721 accctctact ccaacgccct cttccagctc atcccggagg catttggttt caaccctctg
      781 gaagattatt atgtctccaa gtctgcagtg gtgtttgggg gcttttatct tttctttttc
      841 acagagaaga tcttgaagat tcttcttaag cagaaaaatg agcatcatca tggacacagc
      901 cattatgcct ctgagtcgct tccctccaag aaggaccagg aggagggggt gatggagaag
      961 ctgcagaacg gggacctgga ccacatgatt cctcagcact gcagcagtga gctggacggc
     1021 aaggcgccca tggtggacga gaaggtcatt gtgggctcgc tctctgtgca ggacctgcag
     1081 gcttcccaga gtgcttgcta ctggctgaaa ggtgtccgct actctgatat cggcactctg
     1141 gcctggatga tcactctgag cgacggcctc cataatttca tcgatggcct ggccatcggt
     1201 gcttccttca ctgtgtcagt tttccaaggc atcagcacct cggtggccat cctctgtgag
     1261 gagttcccac atgagctagg agactttgtc atcctgctca acgctgggat gagcatccaa
     1321 caagctctct tcttcaactt cctttctgcc tgctgctgct acctgggtct ggcctttggc
     1381 atcctggccg gcagccactt ctctgccaac tggatttttg cgctagctgg aggaatgttc
     1441 ttgtatattt ctctggctga tatgttccct gagatgaatg aggtctgtca agaggatgaa
     1501 aggaagggca gcatcttgat tccatttatc atccagaacc tgggcctcct gactggattc
     1561 accatcatgg tggtcctcac catgtattca ggacagatcc agattggggg atccatcgcc
     1621 accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
     1681 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc
     1741 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
     1801 accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg
     1861 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
     1921 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
     1981 ctggtgaacc gcat
//
