LOCUS       sequence-197807-         382 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Can be used as PCR template for a STAgR reaction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 382)
  AUTHORS   Breunig CT, Durovic T, Neuner AM, Baumann V, Wiesbeck MF, Koferle A,
            Gotz M, Ninkovic J, Stricker SH
  TITLE     One step generation of customizable gRNA vectors for multiplex 
            CRISPR approaches through string assembly gRNA cloning (STAgR).
  JOURNAL   PLoS One. 2018 Apr 27;13(4):e0196015. doi: 
            10.1371/journal.pone.0196015. eCollection 2018.
  PUBMED    29702666
REFERENCE   2  (bases 1 to 382)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2018-04-27"; volume: "13(4):e0196015. doi"; pages: " 
            10.1371/journal.pone.0196015. eCollection 2018"
COMMENT     Sequence Label: STAGR_SAMScaffold_hH1
FEATURES             Location/Qualifiers
     source          1..382
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        18..36
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        88..106
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     promoter        158..372
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     293..312
                     /label=H1
                     /note="Human H1 promoter, forward primer"
ORIGIN
        1 gttttagagc taggccaaca tgaggatcac ccatgtctgc agggcctagc aagttaaaat
       61 aaggctagtc cgttatcaac ttggccaaca tgaggatcac ccatgtctgc agggccaagt
      121 ggcaccgagt cggtgctttt tttcggtgct tttttttgaa cgctgacgtc atcaacccgc
      181 tccaaggaat cgcgggccca gtgtcactag gcgggaacac ccagcgcgcg tgcgccctgg
      241 caggaagatg gctgtgaggg acaggggagt ggcgccctgc aatatttgca tgtcgctatg
      301 tgttctggga aatcaccata aacgtgaaat gtctttggat ttgggaatct tataagttct
      361 gtatgagacc actctttccc ag
//
