LOCUS       sequence-197808-         402 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Can be used as PCR template for a STAgR reaction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 402)
  AUTHORS   Breunig CT, Durovic T, Neuner AM, Baumann V, Wiesbeck MF, Koferle A,
            Gotz M, Ninkovic J, Stricker SH
  TITLE     One step generation of customizable gRNA vectors for multiplex 
            CRISPR approaches through string assembly gRNA cloning (STAgR).
  JOURNAL   PLoS One. 2018 Apr 27;13(4):e0196015. doi: 
            10.1371/journal.pone.0196015. eCollection 2018.
  PUBMED    29702666
REFERENCE   2  (bases 1 to 402)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2018-04-27"; volume: "13(4):e0196015. doi"; pages: " 
            10.1371/journal.pone.0196015. eCollection 2018"
COMMENT     Sequence Label: STAGR_SAMScaffold_h7SK
FEATURES             Location/Qualifiers
     source          1..402
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        18..36
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        88..106
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
ORIGIN
        1 gttttagagc taggccaaca tgaggatcac ccatgtctgc agggcctagc aagttaaaat
       61 aaggctagtc cgttatcaac ttggccaaca tgaggatcac ccatgtctgc agggccaagt
      121 ggcaccgagt cggtgctttt tttcggtgct tttttttctg cagtatttag catgccccac
      181 ccatctgcaa ggcattctgg atagtgtcaa aacagccgga aatcaagtcc gtttatctca
      241 aactttagca ttttgggaat aaatgatatt tgctatgctg gttaaattag attttagtta
      301 aatttcctgc tgaagctcta gtacgataag caacttgacc taagtgtaaa gttgagactt
      361 ccttcaggtt tatatagctt gtgcgccgct tgggtacctc gg
//
