LOCUS       sequence-197809-         473 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Can be used as PCR template for a STAgR reaction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Breunig CT, Durovic T, Neuner AM, Baumann V, Wiesbeck MF, Koferle A,
            Gotz M, Ninkovic J, Stricker SH
  TITLE     One step generation of customizable gRNA vectors for multiplex 
            CRISPR approaches through string assembly gRNA cloning (STAgR).
  JOURNAL   PLoS One. 2018 Apr 27;13(4):e0196015. doi: 
            10.1371/journal.pone.0196015. eCollection 2018.
  PUBMED    29702666
REFERENCE   2  (bases 1 to 473)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2018-04-27"; volume: "13(4):e0196015. doi"; pages: " 
            10.1371/journal.pone.0196015. eCollection 2018"
COMMENT     Sequence Label: STAGR_SAMScaffold_mU6
FEATURES             Location/Qualifiers
     source          1..473
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        18..36
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        88..106
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     promoter        158..471
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for mouse U6 snRNA (Das 
                     et al., 1988)"
     primer_bind     390..410
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
ORIGIN
        1 gttttagagc taggccaaca tgaggatcac ccatgtctgc agggcctagc aagttaaaat
       61 aaggctagtc cgttatcaac ttggccaaca tgaggatcac ccatgtctgc agggccaagt
      121 ggcaccgagt cggtgctttt tttcggtgct tttttttgat ccgacgccgc catctctagg
      181 cccgcgccgg ccccctcgca cagacttgtg ggagaagctc ggctactccc ctgccccggt
      241 taatttgcat ataatatttc ctagtaacta tagaggctta atgtgcgata aaagacagat
      301 aatctgttct ttttaatact agctacattt tacatgatag gcttggattt ctataagaga
      361 tacaaatact aaattattat tttaaaaaac agcacaaaag gaaactcacc ctaactgtaa
      421 agtaattgtg tgttttgaga ctataaatat cccttggaga aaagccttgt ttg
//
