LOCUS       sequence-197827-        1662 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast endogenous mRNA tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Tutucci E, Vera M, Biswas J, Garcia J, Parker R, Singer RH
  TITLE     An improved MS2 system for accurate reporting of the mRNA life 
            cycle.
  JOURNAL   Nat Methods. 2017 Nov 13. doi: 10.1038/nmeth.4502.
  PUBMED    29131164
REFERENCE   2  (bases 1 to 1662)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2017 Nov 13. doi: 10.1038/nmeth.4502."
COMMENT     Sequence Label: pET264-pUC 24xMS2V6 Loxp KANr Loxp
FEATURES             Location/Qualifiers
     source          1..1662
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tccgtgtgag ggtctctgtc gccagagctc ggtattcctg agctcgcgta acaataggaa
       61 tcccagaagt atccgcacga gtgctgatga tatatgcgcg gtagtcctgc gcaaatggtc
      121 ctgatgacga cgacagagca cccatattga agtaatgcaa cggcagggcg tattcgtccc
      181 tgtgaatgct cgcaaggcag atgcaataca cgcaccgggg gccgttttgg ctcgcagggt
      241 attcctctgc gacagtgctg ccatagcaga gtgtaaactt tgcatgggtt ggttgacaga
      301 gaatggggta ttcctccatt ggcaaaaaac ttggcggcca tctgcacacc atgtatgatg
      361 aaggtagtcg agaagcgtaa tcgtcttctc gtacatcggc agaatacctg cacaaccgaa
      421 aagatgaata gaatagaagt ggtcggtagt cctgaccaca cgagtagatt tgactgtacg
      481 agtagacatg ccgatattct gcaccatgca tgcggtaatc ctgcatgacg taagccgact
      541 ccggtatggt ccaattttga cggggaacag agtgtcccac gcggtaatcc tgcgtgtcga
      601 ttgttcggga taatggtgcg atgccccgaa ggtttcctga ctcgcttgtg gggtagtcct
      661 ccacatgtga cactggtgag caatacggaa tgttctttgt agcaccgtgg ggccacacag
      721 ggtattcctc tgtgtcgccc tgggtgacgt tgctccctgc gtataccgcg caagcgagag
      781 tgaagacgcg gtagtcctgc gtctccccgc gattgccagg gggctctggg atcttccgtg
      841 tgagggtctc tgtcgccaga gctcggtatt cctgagctcg cgtaacaata ggaatcccag
      901 aagtatccgc acgagtgctg atgatatatg cgcggtagtc ctgcgcaaat ggtcctgatg
      961 acgacgacag agcacccata ttgaagtaat gcaacggcag ggcgtattcg tccctgtgaa
     1021 tgctcgcaag gcagatgcaa tacacgcacc gggggccgtt ttggctcgca gggtattcct
     1081 ctgcgacagt gctgccatag cagagtgtaa actttgcatg ggttggttga cagagaatgg
     1141 ggtattcctc cattggcaaa aaacttggcg gccatctgca caccatgtat gatgaaggta
     1201 gtcgagaagc gtaatcgtct tctcgtacat cggcagaata cctgcacaac cgaaaagatg
     1261 aatagaatag aagtggtcgg tagtcctgac cacacgagta gatttgactg tacgagtaga
     1321 catgccgata ttctgcacca tgcatgcggt aatcctgcat gacgtaagcc gactccggta
     1381 tggtccaatt ttgacgggga acagagtgtc ccacgcggta atcctgcgtg tcgattgttc
     1441 gggataatgg tgcgatgccc cgaaggtttc ctgactcgct tgtggggtag tcctccacat
     1501 gtgacactgg tgagcaatac ggaatgttct ttgtagcacc gtggggccac acagggtatt
     1561 cctctgtgtc gccctgggtg acgttgctcc ctgcgtatac cgcgcaagcg agagtgaaga
     1621 cgcggtagtc ctgcgtctcc ccgcgattgc cagggggctc tg
//
