LOCUS       sequence-197837-        9529 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Allows co-translational incorporation of the fluorescent ncAA Anap 
            directly into the target protein; expresses amber suppressor tRNA 
            and the synthetase AnapRS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9529)
  AUTHORS   Chatterjee A, Guo J, Lee HS, Schultz PG
  TITLE     A genetically encoded fluorescent probe in Mammalian cells.
  JOURNAL   J Am Chem Soc. 2013 Aug 28;135(34):12540-3. doi: 10.1021/ja4059553. 
            Epub 2013 Aug 14.
  PUBMED    23924161
REFERENCE   2  (bases 1 to 9529)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/ja4059553";
            journalName: "J Am Chem Soc"; date: "2013-08-28- 28"; volume: "135";
            issue: "34"; pages: "12540-3"
COMMENT     Sequence Label: pANAP
FEATURES             Location/Qualifiers
     source          1..9529
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        19..113
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     protein_bind    67..85
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    95..113
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     tRNA            join(118..151,152..154,155..201)
                     /product="amber suppressor variant of an E. coli leucine 
                     tRNA (Wu et al., 2004)"
                     /label=tRNA(CUA)
     promoter        215..309
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     protein_bind    263..281
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    291..309
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     tRNA            join(314..347,348..350,351..397)
                     /product="amber suppressor variant of an E. coli leucine 
                     tRNA (Wu et al., 2004)"
                     /label=tRNA(CUA)
     promoter        411..505
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     protein_bind    459..477
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    487..505
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     tRNA            join(510..543,544..546,547..593)
                     /product="amber suppressor variant of an E. coli leucine 
                     tRNA (Wu et al., 2004)"
                     /label=tRNA(CUA)
     promoter        607..701
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     protein_bind    655..673
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    683..701
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     tRNA            join(706..739,740..742,743..789)
                     /product="amber suppressor variant of an E. coli leucine 
                     tRNA (Wu et al., 2004)"
                     /label=tRNA(CUA)
     promoter        803..897
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     protein_bind    851..869
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    879..897
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     tRNA            join(902..935,936..938,939..985)
                     /product="amber suppressor variant of an E. coli leucine 
                     tRNA (Wu et al., 2004)"
                     /label=tRNA(CUA)
     promoter        999..1093
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     protein_bind    1047..1065
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1075..1093
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     tRNA            join(1098..1131,1132..1134,1135..1181)
                     /product="amber suppressor variant of an E. coli leucine 
                     tRNA (Wu et al., 2004)"
                     /label=tRNA(CUA)
     promoter        1195..1289
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     protein_bind    1243..1261
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1271..1289
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     tRNA            join(1294..1327,1328..1330,1331..1377)
                     /product="amber suppressor variant of an E. coli leucine 
                     tRNA (Wu et al., 2004)"
                     /label=tRNA(CUA)
     promoter        1391..1485
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     protein_bind    1439..1457
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1467..1485
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     tRNA            join(1490..1523,1524..1526,1527..1573)
                     /product="amber suppressor variant of an E. coli leucine 
                     tRNA (Wu et al., 2004)"
                     /label=tRNA(CUA)
     primer_bind     complement(1612..1631)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1803..2182
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2183..2386
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2337..2357
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2431..2450
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2431..2449
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      2485..2494
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2491..5082
                     /codon_start=1
                     /product="aminoacyl-tRNA synthetase specific for the 
                     fluorescent amino acid Anap (Chatterjee et al., 2013)"
                     /label=AnapRS
                     /note="evolved from an E. coli leucyl-tRNA synthetase"
                     /translation="MEEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCFSGPPYPS
                     GRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTYDN
                     IAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCPNDQ
                     TVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKTMQRN
                     WIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNPELAAF
                     IDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGAVMAVPG
                     HDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDHEAAFNAI
                     ADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQLPVILPEDV
                     VMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWVLARYTCPQYKEGMLDSEA
                     ANYWLPVDIAIGGIEHAIMESLYCRFFHKLMRDAGMVNSDEPVKQLLCQGMVLADAFYY
                     VGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSKNNGIDPQVMVE
                     RYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTAKSDVAALNVDAL
                     TENQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAPTDGEQDRALMQEA
                     LLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVEDSTLVVVQVNGKVRA
                     KITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLNLVVGGPV"
     primer_bind     complement(5120..5137)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5126..5350
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5396..5824
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5483..5502)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5693..5714
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5833..5853)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5838..6167
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6018..6153
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6080..6099
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6234..7028
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6288..6307)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6898..6917
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7202..7323
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7239..7258)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7293..7312
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7372..7388)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7372..7388)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7385..7407)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7396..7412
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7420..7426,7427..7444,7445..7450))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7465..7486
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7603..7620)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7774..8362)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7854..7873)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8533..9324,9325..9393))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9156..9175
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9394..9498)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctaat atttgcatgt cgctatgtgt tctgggaaat caccataaac
       61 gtgaaatccc tatcagtgat agagacttat aagttcccta tcagtgatag agacaccgcc
      121 cggatggtgg aatcggtaga cacaagggat tctaaatccc tcggcgttcg cgctgtgcgg
      181 gttcaagtcc cgctccgggt attttttgga tctaatattt gcatgtcgct atgtgttctg
      241 ggaaatcacc ataaacgtga aatccctatc agtgatagag acttataagt tccctatcag
      301 tgatagagac accgcccgga tggtggaatc ggtagacaca agggattcta aatccctcgg
      361 cgttcgcgct gtgcgggttc aagtcccgct ccgggtattt tttggatcta atatttgcat
      421 gtcgctatgt gttctgggaa atcaccataa acgtgaaatc cctatcagtg atagagactt
      481 ataagttccc tatcagtgat agagacaccg cccggatggt ggaatcggta gacacaaggg
      541 attctaaatc cctcggcgtt cgcgctgtgc gggttcaagt cccgctccgg gtattttttg
      601 gatctaatat ttgcatgtcg ctatgtgttc tgggaaatca ccataaacgt gaaatcccta
      661 tcagtgatag agacttataa gttccctatc agtgatagag acaccgcccg gatggtggaa
      721 tcggtagaca caagggattc taaatccctc ggcgttcgcg ctgtgcgggt tcaagtcccg
      781 ctccgggtat tttttggatc taatatttgc atgtcgctat gtgttctggg aaatcaccat
      841 aaacgtgaaa tccctatcag tgatagagac ttataagttc cctatcagtg atagagacac
      901 cgcccggatg gtggaatcgg tagacacaag ggattctaaa tccctcggcg ttcgcgctgt
      961 gcgggttcaa gtcccgctcc gggtattttt tggatctaat atttgcatgt cgctatgtgt
     1021 tctgggaaat caccataaac gtgaaatccc tatcagtgat agagacttat aagttcccta
     1081 tcagtgatag agacaccgcc cggatggtgg aatcggtaga cacaagggat tctaaatccc
     1141 tcggcgttcg cgctgtgcgg gttcaagtcc cgctccgggt attttttgga tctaatattt
     1201 gcatgtcgct atgtgttctg ggaaatcacc ataaacgtga aatccctatc agtgatagag
     1261 acttataagt tccctatcag tgatagagac accgcccgga tggtggaatc ggtagacaca
     1321 agggattcta aatccctcgg cgttcgcgct gtgcgggttc aagtcccgct ccgggtattt
     1381 tttggatcta atatttgcat gtcgctatgt gttctgggaa atcaccataa acgtgaaatc
     1441 cctatcagtg atagagactt ataagttccc tatcagtgat agagacaccg cccggatggt
     1501 ggaatcggta gacacaaggg attctaaatc cctcggcgtt cgcgctgtgc gggttcaagt
     1561 cccgctccgg gtattttttg gatctcccga tcccctatgg tgcactctca gtacaatctg
     1621 ctctgatgcc gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga
     1681 gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa
     1741 gaatctgctt agggttaggc gttttgcgct gcttcgcgat gtacgggcca gatatacgcg
     1801 ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag
     1861 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
     1921 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
     1981 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
     2041 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
     2101 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
     2161 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
     2221 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
     2281 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
     2341 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctctct ggctaactag
     2401 agaacccact gcttactggc ttatcgaaat taatacgact cactataggg agacccaagc
     2461 tggctagcgt ttaaacttaa gcttgccacc atggaagagc aataccgccc ggaagagata
     2521 gaatccaaag tacagcttca ttgggatgag aagcgcacat ttgaagtaac cgaagacgag
     2581 agcaaagaga agtattactg cttttctggc cctccctatc cttctggtcg actacacatg
     2641 ggccacgtac gtaactacac catcggtgac gtgatcgccc gctaccagcg tatgctgggc
     2701 aaaaacgtcc tgcagccgat cggctgggac gcgtttggtc tgcctgcgga aggcgcggcg
     2761 gtgaaaaaca acaccgctcc ggcaccgtgg acgtacgaca acatcgcgta tatgaaaaac
     2821 cagctcaaaa tgctgggctt tggttatgac tggagccgcg agctggcaac ctgtacgccg
     2881 gaatactacc gttgggaaca gaaattcttc accgagctgt ataaaaaagg cctggtatat
     2941 aagaagactt ctgcggtcaa ctggtgtccg aacgaccaga ccgtactggc gaacgaacaa
     3001 gttatcgacg gctgctgctg gcgctgcgat accaaagttg aacgtaaaga gatcccgcag
     3061 tggtttatca aaatcactgc ttacgctgac gagctgctca acgatctgga taaactggat
     3121 cactggcctg acaccgttaa aaccatgcag cgtaactgga tcggtcgttc cgaaggcgtg
     3181 gagatcacct tcaacgttaa cgactatgac aacacgctga ccgtttacac tacccgcccg
     3241 gacaccttta tgggttgtac ctacctggcg gtagctgcgg gtcatccgct ggcgcagaaa
     3301 gcggcggaaa ataatcctga actggcggcc tttattgacg aatgccgtaa caccaaagtt
     3361 gccgaagctg aaatggcgac gatggagaaa aaaggcgtcg atactggctt taaagcggtt
     3421 cacccattaa cgggcgaaga aattcccgtt tgggcagcaa acttcgtatt gatggagtac
     3481 ggcacgggcg cagttatggc ggtaccgggg cacgaccagc gcgactacga gtttgcctct
     3541 aaatacggcc tgaacatcaa accggttatc ctggcagctg acggctctga gccagatctt
     3601 tctcagcaag ccctgactga aaaaggcgtg ctgttcaact ctggcgagtt caacggtctt
     3661 gaccatgaag cggccttcaa cgccatcgcc gataaactga ctgcgatggg cgttggcgag
     3721 cgtaaagtga actaccgcct gcgcgactgg ggtgtttccc gtcagcgtta ctggggcgcg
     3781 ccgattccga tggtgactct agaagacggt accgtaatgc cgaccccgga cgaccagctg
     3841 ccggtgatcc tgccggagga tgtggtaatg gacggcatta ccagcccgat taaagcagat
     3901 ccggagtggg cgaaaactac cgttaacggt atgccagcac tgcgtgaaac cgacactttc
     3961 gacaccttta tggagtcctc ctgggttctt gcgcgctaca cttgcccgca gtacaaagaa
     4021 ggtatgctgg attccgaagc ggctaactac tggctgccgg tggatatcgc gattggtggt
     4081 attgaacacg ccattatgga gagtctctac tgtcgcttct tccacaaact gatgcgtgat
     4141 gcaggcatgg tgaactctga cgaaccagtt aaacagttgc tgtgtcaggg tatggtgctg
     4201 gcagatgcct tctactatgt tggcgaaaac ggcgaacgta actgggtttc cccggttgat
     4261 gctatcgttg aacgtgacga gaaaggccgt atcgtgaaag cgaaagatgc ggcaggccat
     4321 gaactggttt ataccggcat gagcaaaatg tccaagtcga agaacaacgg tatcgacccg
     4381 caggtgatgg ttgaacgtta cggcgcggac accgttcgtc tgtttatgat gtttgcttct
     4441 ccggctgata tgactctcga atggcaggaa tccggtgtgg aaggggctaa ccgcttcctg
     4501 aaacgtgtct ggaaactggt ttacgagcac acagcaaaaa gtgatgttgc ggcactgaac
     4561 gttgatgcgc tgactgaaaa tcagaaagcg ctgcgtcgcg atgtgcataa aacgatcgct
     4621 aaagtgaccg atgatatcgg ccgtcgtcag accttcaaca ccgcaattgc ggcgattatg
     4681 gagctgatga acaaactggc gaaagcacca accgatggcg agcaggatcg cgctctgatg
     4741 caggaagcac tgctggccgt tgtccgtatg cttaacccgt tcaccccgca catctgcttc
     4801 acgctgtggc aggaactgaa aggcgaaggc gatatcgaca acgcgccgtg gccggttgct
     4861 gacgaaaaag cgatggtgga agactccacg ctggtcgtgg tgcaggttaa cggtaaagtc
     4921 cgtgccaaaa tcaccgttcc ggtggacgca acggaagaac aggttcgcga acgtgctggc
     4981 caggaacatc tggtagcaaa atatcttgat ggcgttactg tacgtaaagt gatttacgta
     5041 ccaggtaaac tcctcaatct ggtcgttggc gggcccgttt aactcgagtc tagagggccc
     5101 gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
     5161 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
     5221 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
     5281 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
     5341 ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg
     5401 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     5461 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     5521 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     5581 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     5641 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     5701 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     5761 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     5821 aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
     5881 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     5941 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     6001 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     6061 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat
     6121 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag
     6181 cttgtatatc cattttcgga tctgatcaag agacaggatg aggatcgttt cgcatgattg
     6241 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg
     6301 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg
     6361 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg
     6421 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg
     6481 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc
     6541 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc
     6601 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc
     6661 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc
     6721 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg
     6781 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct
     6841 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt
     6901 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc
     6961 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt
     7021 tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc
     7081 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg
     7141 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc
     7201 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     7261 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     7321 ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct
     7381 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     7441 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     7501 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     7561 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     7621 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     7681 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     7741 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     7801 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     7861 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     7921 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     7981 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     8041 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     8101 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     8161 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     8221 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     8281 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     8341 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     8401 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     8461 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     8521 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     8581 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     8641 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     8701 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     8761 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     8821 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     8881 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     8941 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     9001 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     9061 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     9121 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     9181 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     9241 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     9301 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     9361 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     9421 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     9481 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtc
//
