LOCUS       sequence-197842-       13886 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  fluorescent tagging. Plant expression of EFR Y877F (17).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13886)
  TITLE     Robatzek Lab plasmids
REFERENCE   2  (bases 1 to 13886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: EFR Y877F (17)
FEATURES             Location/Qualifiers
     source          1..13886
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        47..392
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     335..355
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    404..428
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3558..3582)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3605..4315
                     /codon_start=1
                     /gene="mgfp5"
                     /product="GFP with folding enhancement mutations"
                     /label=mgfp5
                     /note="suitable for expression in plants due to removal of 
                     a cryptic intron"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKAN
                     FKTRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(3632..3660)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     4255..4275
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             4322..4339
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      4397..5104
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     primer_bind     complement(5129..5146)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5129..5145)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5138..5160)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    5348..5372
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6673..7302
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             7731..8804
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      8870..9064
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     misc_feature    9408..9548
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     9463..9482
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(9563..9580)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9734..10322)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9814..9833)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(10409..11203)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    11628..11652
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(11738..11990)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     CDS             complement(12000..12551)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(12557..12937)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
ORIGIN
        1 actccaaaaa tgtcaaagat acagtctcag aagaccaaag ggctattgag acttttcaac
       61 aaaggataat ttcgggaaac ctcctcggat tccattgccc agctatctgt cacttcatcg
      121 aaaggacagt agaaaaggaa ggtggctcct acaaatgcca tcattgcgat aaaggaaagg
      181 ctatcattca agatctctct gccgacagtg gtcccaaaga tggaccccca cccacgagga
      241 gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat tgatgtgaca
      301 tctccactga cgtaagggat gacgcacaat cccactatcc ttcgcaagac ccttcctcta
      361 tataaggaag ttcatttcat ttggagagga cacgctcgag atcacaagtt tgtacaaaaa
      421 agcaggctcc gcggccgccc ccttcaccat gaagctgtcc ttttcacttg ttttcaatgc
      481 tctcacgttg cttcttcaag tttgcatctt tgctcaagcc aggttttcta atgagactga
      541 tatgcaagct ttgcttgagt tcaagtctca agtttctgaa aacaacaaga gagaggtctt
      601 ggcttcatgg aatcactcct ccccattttg taattggatt ggagtcacat gtggccgcag
      661 gagagaaaga gttataagtt tgaaccttgg aggattcaag ttaaccggtg tgatctcacc
      721 ctccattggt aatctctcct ttcttagatt acttaatctt gcagacaact cttttggaag
      781 taccatccct caaaaggtgg gaaggctatt taggcttcag tacttgaaca tgagctataa
      841 tcttctcgaa ggaaggattc cgtctagtct ttctaactgc tctagactgt cgaccgttga
      901 tttatcgtca aaccatcttg gacatggtgt tccttcagaa ctaggttcac tttctaagct
      961 tgccattctg gatcttagca aaaacaacct tactggaaat tttcctgcat ctttaggaaa
     1021 cttgacgtca cttcagaagc ttgactttgc atataaccag atgagaggtg agattccaga
     1081 cgaagtagct agattgactc aaatggtgtt tttccaaata gcactgaata gtttttcagg
     1141 tggttttcct cctgcattgt acaacatctc ctctcttgag tctctatctc tagctgacaa
     1201 tagcttttcg ggtaatctta gggctgattt tggttatctt ctaccaaatc taagaagact
     1261 tcttttggga acaaatcagt tcactggagc tattcccaaa acacttgcca atatctcaag
     1321 ccttgaaagg tttgatatct catctaatta cctgtctggt agtatccctt tgagctttgg
     1381 aaagttacgt aatctgtggt ggttagggat tcgtaataac tctcttggaa ataactcgtc
     1441 cagtggtctt gaatttattg gggctgtggc gaactgcact caattagagt acttagatgt
     1501 tggttacaat agacttggag gtgagcttcc tgcttctata gccaatctgt ccactacatt
     1561 gactagtctg ttccttggac aaaatcttat ctctggaacc attcctcatg acatcgggaa
     1621 tcttgtaagc ctgcaagaac tcagcttaga aacaaatatg ttgagtggag aacttcccgt
     1681 ctctttcggg aagcttttga acttgcaggt tgtggatctg tattcaaatg caatatcggg
     1741 ggaaatacca tcttattttg gcaacatgac tcggttgcag aagctccatt tgaatagcaa
     1801 tagtttccac ggaagaatcc ctcagagtct tggacgttgt cgatacttgc tagacctgtg
     1861 gatggataca aataggttga atgggactat acctcaggaa atactgcaaa ttccatccct
     1921 cgcttacata gatttgtcaa acaatttctt gacaggccat tttccagaag aagttggaaa
     1981 gttagaactt cttgttggac taggtgcttc gtacaacaaa ttatcaggaa agatgccaca
     2041 agctataggg ggttgtcttt cgatggaatt tctctttatg caaggaaatt cgtttgacgg
     2101 agccattcca gatataagcc ggttggtaag cctaaagaat gttgacttct ccaacaacaa
     2161 tctctctggc cggatacctc gatatctggc cagtcttcct tcgctgcgaa atctgaatct
     2221 ttctatgaac aagtttgagg gaagggtgcc aacaacagga gtgtttcgaa atgctacagc
     2281 agtttctgtt tttggtaaca caaatatttg cggaggcgtc cgagaaatgc aactaaagcc
     2341 atgcattgta caggcatctc caaggaagag aaagcctctg tcagttagaa agaaagttgt
     2401 cagtggtatt tgtataggta tagcttcgct tttgttaatc ataattgtgg cttctctgtg
     2461 ttggttcatg aagaggaaaa agaaaaacaa tgccagtgat ggtaacccat ctgattctac
     2521 tactttgggg atgttccatg agaaggtaag ttatgaagag cttcatagtg caacaagtcg
     2581 cttctcttca accaatttga ttggttcagg caatttcggt aatgtgttta aaggattgct
     2641 tggccctgag aataaactcg tcgcggttaa agttttgaac ctcctaaagc atggagcgac
     2701 gaaaagcttt atggcggaat gtgaaacctt caagggtata cgacatcgta accttgtaaa
     2761 actgataacg gtttgttcaa gccttgattc cgagggaaat gatttcagag ctctggtcta
     2821 tgagttcatg ccaaaaggaa gtctggatat gtggctgcag ctagaagatc tggaaagggt
     2881 aaacgatcac tcgagatctt taacacccgc agagaaactc aacatagcaa tagatgtggc
     2941 ttcagctttg gagtatctgc acgttcattg tcatgaccct gtagctcact gtgatattaa
     3001 gccaagcaac attcttctag acgatgatct gactgctcat gttagtgact ttggtttggc
     3061 tcagctcctc tataaattcg atcgagaatc ctttctaaac cagtttagtt ctgctggtgt
     3121 cagaggcacc attggctatg ccgcgccaga gtatggaatg ggaggccaac catcaataca
     3181 aggagatgtg tacagcttcg gaattctact tttggagatg tttagtggaa agaaaccaac
     3241 agatgaatca tttgcaggcg attataacct ccacagctac acaaagtcta tattatcggg
     3301 ttgcacgagc agtggaggca gcaacgccat tgatgagggg ttgagactgg ttttgcaggt
     3361 ggggataaag tgttctgaag aatatccgag ggataggatg agaacggatg aagcagtacg
     3421 agaattaatc tcaataagat ctaagttctt cagttccaag acgactatta cagagagtcc
     3481 tcgagatgct ccgcaaagtt ctcctcagga atggatgtta aatacggaca tgcatactat
     3541 gaagggtggg cgcgccgacc cagctttctt gtacaaagtg gtgctcgaga tggtagatct
     3601 gactagtaaa ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg
     3661 tgatgttaat gggcacaaat tttctgtcag tggagagggt gaaggtgatg caacatacgg
     3721 aaaacttacc cttaaattta tttgcactac tggaaaacta cctgttccgt ggccaacact
     3781 tgtcactact ttctcttatg gtgttcaatg cttttcaaga tacccagatc atatgaagcg
     3841 gcacgacttc ttcaagagcg ccatgcctga gggatacgtg caggagagga ccatcttctt
     3901 caaggacgac gggaactaca agacacgtgc tgaagtcaag tttgagggag acaccctcgt
     3961 caacaggatc gagcttaagg gaatcgattt caaggaggac ggaaacatcc tcggccacaa
     4021 gttggaatac aactacaact cccacaacgt atacatcatg gccgacaagc aaaagaacgg
     4081 catcaaagcc aacttcaaga cccgccacaa catcgaagac ggcggcgtgc aactcgctga
     4141 tcattatcaa caaaatactc caattggcga tggccctgtc cttttaccag acaaccatta
     4201 cctgtccaca caatctgccc tttcgaaaga tcccaacgaa aagagagacc acatggtcct
     4261 tcttgagttt gtaacagctg ctgggattac acatggcatg gatgaactat acaaagctag
     4321 ccaccaccac caccaccacg tgtgacctag gtgagtctag agagttaatt aagacccggg
     4381 actagtccct agagtcctgc tttaatgaga tatgcgagac gcctatgatc gcatgatatt
     4441 tgctttcaat tctgttgtgc acgttgtaaa aaacctgagc atgtgtagct cagatcctta
     4501 ccgccggttt cggttcattc taatgaatat atcacccgtt actatcgtat ttttatgaat
     4561 aatattctcc gttcaattta ctgattgtac cctactactt atatgtacaa tattaaaatg
     4621 aaaacaatat attgtgctga ataggtttat agcgacatct atgatagagc gccacaataa
     4681 caaacaattg cgttttatta ttacaaatcc aattttaaaa aaagcggcag aaccggtcaa
     4741 acctaaaaga ctgattacat aaatcttatt caaatttcaa aagtgcccca ggggctagta
     4801 tctacgacac accgagcggc gaactaataa cgctcactga agggaactcc ggttccccgc
     4861 cggcgcgcat gggtgagatt ccttgaagtt gagtattggc cgtccgctct accgaaagtt
     4921 acgggcacca ttcaacccgg tccagcacgg cggccgggta accgacttgc tgccccgaga
     4981 attatgcagc atttttttgg tgtatgtggg ccccaaatga agtgcaggtc aaaccttgac
     5041 agtgacgaca aatcgttggg cgggtccagg gcgaattttg cgacaacatg tcgaggctca
     5101 gcaggacctg caggcatgca agcttggcac tggccgtcgt tttacaacgt cgtgactggg
     5161 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
     5221 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
     5281 aatgctagag cagcttgagc ttggatcaga ttgtcgtttc ccgccttcag tttaaactat
     5341 cagtgtttga caggatatat tggcgggtaa acctaagaga aaagagcgtt tattagaata
     5401 atcggatatt taaaagggcg tgaaaaggtt tatccgttcg tccatttgta tgtgcatgcc
     5461 aaccacaggg ttcccctcgg gatcaaagta ctttgatcca acccctccgc tgctatagtg
     5521 cagtcggctt ctgacgttca gtgcagccgt cttctgaaaa cgacatgtcg cacaagtcct
     5581 aagttacgcg acaggctgcc gccctgccct tttcctggcg ttttcttgtc gcgtgtttta
     5641 gtcgcataaa gtagaatact tgcgactaga accggagaca ttacgccatg aacaagagcg
     5701 ccgccgctgg cctgctgggc tatgcccgcg tcagcaccga cgaccaggac ttgaccaacc
     5761 aacgggccga actgcacgcg gccggctgca ccaagctgtt ttccgagaag atcaccggca
     5821 ccaggcgcga ccgcccggag ctggccagga tgcttgacca cctacgccct ggcgacgttg
     5881 tgacagtgac caggctagac cgcctggccc gcagcacccg cgacctactg gacattgccg
     5941 agcgcatcca ggaggccggc gcgggcctgc gtagcctggc agagccgtgg gccgacacca
     6001 ccacgccggc cggccgcatg gtgttgaccg tgttcgccgg cattgccgag ttcgagcgtt
     6061 ccctaatcat cgaccgcacc cggagcgggc gcgaggccgc caaggcccga ggcgtgaagt
     6121 ttggcccccg ccctaccctc accccggcac agatcgcgca cgcccgcgag ctgatcgacc
     6181 aggaaggccg caccgtgaaa gaggcggctg cactgcttgg cgtgcatcgc tcgaccctgt
     6241 accgcgcact tgagcgcagc gaggaagtga cgcccaccga ggccaggcgg cgcggtgcct
     6301 tccgtgagga cgcattgacc gaggccgacg ccctggcggc cgccgagaat gaacgccaag
     6361 aggaacaagc atgaaaccgc accaggacgg ccaggacgaa ccgtttttca ttaccgaaga
     6421 gatcgaggcg gagatgatcg cggccgggta cgtgttcgag ccgcccgcgc acgtctcaac
     6481 cgtgcggctg catgaaatcc tggccggttt gtctgatgcc aagctggcgg cctggccggc
     6541 cagcttggcc gctgaagaaa ccgagcgccg ccgtctaaaa aggtgatgtg tatttgagta
     6601 aaacagcttg cgtcatgcgg tcgctgcgta tatgatgcga tgagtaaata aacaaatacg
     6661 caaggggaac gcatgaaggt tatcgctgta cttaaccaga aaggcgggtc aggcaagacg
     6721 accatcgcaa cccatctagc ccgcgccctg caactcgccg gggccgatgt tctgttagtc
     6781 gattccgatc cccagggcag tgcccgcgat tgggcggccg tgcgggaaga tcaaccgcta
     6841 accgttgtcg gcatcgaccg cccgacgatt gaccgcgacg tgaaggccat cggccggcgc
     6901 gacttcgtag tgatcgacgg agcgccccag gcggcggact tggctgtgtc cgcgatcaag
     6961 gcagccgact tcgtgctgat tccggtgcag ccaagccctt acgacatatg ggccaccgcc
     7021 gacctggtgg agctggttaa gcagcgcatt gaggtcacgg atggaaggct acaagcggcc
     7081 tttgtcgtgt cgcgggcgat caaaggcacg cgcatcggcg gtgaggttgc cgaggcgctg
     7141 gccgggtacg agctgcccat tcttgagtcc cgtatcacgc agcgcgtgag ctacccaggc
     7201 actgccgccg ccggcacaac cgttcttgaa tcagaacccg agggcgacgc tgcccgcgag
     7261 gtccaggcgc tggccgctga aattaaatca aaactcattt gagttaatga ggtaaagaga
     7321 aaatgagcaa aagcacaaac acgctaagtg ccggccgtcc gagcgcacgc agcagcaagg
     7381 ctgcaacgtt ggccagcctg gcagacacgc cagccatgaa gcgggtcaac tttcagttgc
     7441 cggcggagga tcacaccaag ctgaagatgt acgcggtacg ccaaggcaag accattaccg
     7501 agctgctatc tgaatacatc gcgcagctac cagagtaaat gagcaaatga ataaatgagt
     7561 agatgaattt tagcggctaa aggaggcggc atggaaaatc aagaacaacc aggcaccgac
     7621 gccgtggaat gccccatgtg tggaggaacg ggcggttggc caggcgtaag cggctgggtt
     7681 gcctgccggc cctgcaatgg cactggaacc cccaagcccg aggaatcggc gtgagcggtc
     7741 gcaaaccatc cggcccggta caaatcggcg cggcgctggg tgatgacctg gtggagaagt
     7801 tgaaggccgc gcaggccgcc cagcggcaac gcatcgaggc agaagcacgc cccggtgaat
     7861 cgtggcaagc ggccgctgat cgaatccgca aagaatcccg gcaaccgccg gcagccggtg
     7921 cgccgtcgat taggaagccg cccaagggcg acgagcaacc agattttttc gttccgatgc
     7981 tctatgacgt gggcacccgc gatagtcgca gcatcatgga cgtggccgtt ttccgtctgt
     8041 cgaagcgtga ccgacgagct ggcgaggtga tccgctacga gcttccagac gggcacgtag
     8101 aggtttccgc agggccggcc ggcatggcca gtgtgtggga ttacgacctg gtactgatgg
     8161 cggtttccca tctaaccgaa tccatgaacc gataccggga agggaaggga gacaagcccg
     8221 gccgcgtgtt ccgtccacac gttgcggacg tactcaagtt ctgccggcga gccgatggcg
     8281 gaaagcagaa agacgacctg gtagaaacct gcattcggtt aaacaccacg cacgttgcca
     8341 tgcagcgtac gaagaaggcc aagaacggcc gcctggtgac ggtatccgag ggtgaagcct
     8401 tgattagccg ctacaagatc gtaaagagcg aaaccgggcg gccggagtac atcgagatcg
     8461 agctagctga ttggatgtac cgcgagatca cagaaggcaa gaacccggac gtgctgacgg
     8521 ttcaccccga ttactttttg atcgatcccg gcatcggccg ttttctctac cgcctggcac
     8581 gccgcgccgc aggcaaggca gaagccagat ggttgttcaa gacgatctac gaacgcagtg
     8641 gcagcgccgg agagttcaag aagttctgtt tcaccgtgcg caagctgatc gggtcaaatg
     8701 acctgccgga gtacgatttg aaggaggagg cggggcaggc tggcccgatc ctagtcatgc
     8761 gctaccgcaa cctgatcgag ggcgaagcat ccgccggttc ctaatgtacg gagcagatgc
     8821 tagggcaaat tgccctagca ggggaaaaag gtcgaaaagg tctctttcct gtggatagca
     8881 cgtacattgg gaacccaaag ccgtacattg ggaaccggaa cccgtacatt gggaacccaa
     8941 agccgtacat tgggaaccgg tcacacatgt aagtgactga tataaaagag aaaaaaggcg
     9001 atttttccgc ctaaaactct ttaaaactta ttaaaactct taaaacccgc ctggcctgtg
     9061 cataactgtc tggccagcgc acagccgaag agctgcaaaa agcgcctacc cttcggtcgc
     9121 tgcgctccct acgccccgcc gcttcgcgtc ggcctatcgc ggccgctggc cgctcaaaaa
     9181 tggctggcct acggccaggc aatctaccag ggcgcggaca agccgcgccg tcgccactcg
     9241 accgccggcg cccacatcaa ggcaccctgc ctcgcgcgtt tcggtgatga cggtgaaaac
     9301 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     9361 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc
     9421 cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg
     9481 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
     9541 gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     9601 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     9661 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     9721 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     9781 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     9841 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     9901 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     9961 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
    10021 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
    10081 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
    10141 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
    10201 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
    10261 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
    10321 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
    10381 aagggatttt ggtcatgcat tctaggtact aaaacaattc atccagtaaa atataatatt
    10441 ttattttctc ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg acatactgtt
    10501 cttccccgat atcctccctg atcgaccgga cgcagaaggc aatgtcatac cacttgtccg
    10561 ccctgccgct tctcccaaga tcaataaagc cacttacttt gccatctttc acaaagatgt
    10621 tgctgtctcc caggtcgccg tgggaaaaga caagttcctc ttcgggcttt tccgtcttta
    10681 aaaaatcata cagctcgcgc ggatctttaa atggagtgtc ttcttcccag ttttcgcaat
    10741 ccacatcggc cagatcgtta ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc
    10801 tattcgtata gggacaatcc gatatgtcga tggagtgaaa gagcctgatg cactccgcat
    10861 acagctcgat aatcttttca gggctttgtt catcttcata ctcttccgag caaaggacgc
    10921 catcggcctc actcatgagc agattgctcc agccatcatg ccgttcaaag tgcaggacct
    10981 ttggaacagg cagctttcct tccagccata gcatcatgtc cttttcccgt tccacatcat
    11041 aggtggtccc tttataccgg ctgtccgtca tttttaaata taggttttca ttttctccca
    11101 ccagcttata taccttagca ggagacattc cttccgtatc ttttacgcag cggtattttt
    11161 cgatcagttt tttcaattcc ggtgatattc tcattttagc catttattat ttccttcctc
    11221 ttttctacag tatttaaaga taccccaaga agctaattat aacaagacga actccaattc
    11281 actgttcctt gcattctaaa accttaaata ccagaaaaca gctttttcaa agttgttttc
    11341 aaagttggcg tataacatag tatcgacgga gccgattttg aaaccgcggt gatcacaggc
    11401 agcaacgctc tgtcatcgtt acaatcaaca tgctaccctc cgcgagatca tccgtgtttc
    11461 aaacccggca gcttagttgc cgttcttccg aatagcatcg gtaacatgag caaagtctgc
    11521 cgccttacaa cggctctccc gctgacgccg tcccggactg atgggctgcc tgtatcgagt
    11581 ggtgattttg tgccgagctg ccggtcgggg agctgttggc tggctggtgg caggatatat
    11641 tgtggtgtaa acaaattgac gcttagacaa cttaataaca cattgcggac gtttttaatg
    11701 tactgaatta acgccgaatt aattcgagct cggatctgat aatttatttg aaaattcata
    11761 agaaaagcaa acgttacatg aattgatgaa acaatacaaa gacagataaa gccacgcaca
    11821 tttaggatat tggccgagat tactgaatat tgagtaagat cacggaattt ctgacaggag
    11881 catgtcttca attcagccca aatggcagtt gaaatactca aaccgcccca tatgcaggag
    11941 cggatcattc attgtttgtt tggttgcctt tgccaacatg ggagtccaag attctgcagt
    12001 caaatctcgg tgacgggcag gaccggacgg ggcggtaccg gcaggctgaa gtccagctgc
    12061 cagaaaccca cgtcatgcca gttcccgtgc ttgaagccgg ccgcccgcag catgccgcgg
    12121 ggggcatatc cgagcgcctc gtgcatgcgc acgctcgggt cgttgggcag cccgatgaca
    12181 gcgaccacgc tcttgaagcc ctgtgcctcc agggacttca gcaggtgggt gtagagcgtg
    12241 gagcccagtc ccgtccgctg gtggcggggg gagacgtaca cggtcgactc ggccgtccag
    12301 tcgtaggcgt tgcgtgcctt ccaggggccc gcgtaggcga tgccggcgac ctcgccgtcc
    12361 acctcggcga cgagccaggg atagcgctcc cgcagacgga cgaggtcgtc cgtccactcc
    12421 tgcggttcct gcggctcggt acggaagttg accgtgcttg tctcgatgta gtggttgacg
    12481 atggtgcaga ccgccggcat gtccgcctcg gtggcacggc ggatgtcggc cgggcgtcgt
    12541 tctgggctca tcgattcgat ttggtgtatc gagattggtt atgaaattca gatgctagtg
    12601 taatgtattg gtaatttggg aagatataat aggaagcaag gctatttatc catttctgaa
    12661 aaggcgaaat ggcgtcaccg cgagcgtcac gcgcattccg ttcttgctgt aaagcgttgt
    12721 ttggtacact tttgactagc gaggcttggc gtgtcagcgt atctattcaa aagtcgttaa
    12781 tggctgcgga tcaagaaaaa gttggaatag aaacagaata cccgcgaaat tcaggcccgg
    12841 ttgccatgtc ctacacgccg aaataaacga ccaaattagt agaaaaataa aaactgactc
    12901 ggatacttac gtcacgtctt gcgcactgat ttgaaaaatc tcagaattcc aatcccacaa
    12961 aaatctgagc ttaacagcac agttgctcct ctcagagcag aatcgggtat tcaacaccct
    13021 catatcaact actacgttgt gtataacggt ccacatgccg gtatatacga tgactggggt
    13081 tgtacaaagg cggcaacaaa cggcgttccc ggagttgcac acaagaaatt tgccactatt
    13141 acagaggcaa gagcagcagc tgacgcgtac acaacaagtc agcaaacaga caggttgaac
    13201 ttcatcccca aaggagaagc tcaactcaag cccaagagct ttgctaaggc cctaacaagc
    13261 ccaccaaagc aaaaagccca ctggctcacg ctaggaacca aaaggcccag cagtgatcca
    13321 gccccaaaag agatctcctt tgccccggag attacaatgg acgatttcct ctatctttac
    13381 gatctaggaa ggaagttcga aggtgaaggt gacgacacta tgttcaccac tgataatgag
    13441 aaggttagcc tcttcaattt cagaaagaat gctgacccac agatggttag agaggcctac
    13501 gcagcaggtc tcatcaagac gatctacccg agtaacaatc tccaggagat caaatacctt
    13561 cccaagaagg ttaaagatgc agtcaaaaga ttcaggacta attgcatcaa gaacacagag
    13621 aaagacatat ttctcaagat cagaagtact attccagtat ggacgattca aggcttgctt
    13681 cataaaccaa ggcaagtaat agagattgga gtctctaaaa aggtagttcc tactgaatct
    13741 aaggccatgc atggagtcta agattcaaat cgaggatcta acagaactcg ccgtgaagac
    13801 tggcgaacag ttcatacaga gtcttttacg actcaatgac aagaagaaaa tcttcgtcaa
    13861 catggtggag cacgacactc tggtct
//
