LOCUS       sequence-197847-        6878 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Reporter for translation activation via Pum..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6878)
  AUTHORS   Adamala KP, Martin-Alarcon DA, Boyden ES
  TITLE     Programmable RNA-binding protein composed of repeats of a single 
            modular unit.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 May 10;113(19):E2579-88. doi: 
            10.1073/pnas.1519368113. Epub 2016 Apr 26.
  PUBMED    27118836
REFERENCE   2  (bases 1 to 6878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1519368113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-05-10- 10"; volume: "113"; issue: "19"; pages: 
            "E2579-88"
COMMENT     Sequence Label: [UBC][firefly luciferase][target cloning][reni...
FEATURES             Location/Qualifiers
     source          1..6878
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        20..38
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      45..54
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1782..1791
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    2747..2868
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2758..2777)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2812..2831
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      3049..3504
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3136..3155)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3346..3367
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3571..3590)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3690..3712
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3750..3768)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3836..3940
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3941..4009,4010..4801)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4159..4178)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4972..5560
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5461..5480
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        5642..6853
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     6690..6709
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
ORIGIN
        1 ctcttaaggc tagagtactt aatacgactc actataggct agccgccacc atggctcgga
       61 tggaggacgc taaaaatatt aagaaaggac cggctccatt ctaccctttg gaggacggaa
      121 cagcaggcga gcagctgcac aaggcaatga aacgctacgc tctggttcca ggtacaattg
      181 ctttcacaga tgcccacatc gaagtggaca taacctatgc cgaatacttt gaaatgtccg
      241 tgcggctcgc ggaagccatg aagcgatatg ggctcaacac caaccaccga atcgttgtgt
      301 gcagtgagaa ctctctgcaa ttctttatgc cagtgttggg cgcgctcttt atcggcgtgg
      361 ccgttgcacc tgcaaatgat atttacaacg agcgggaact gctgaatagc atgggaatct
      421 cccagcctac tgttgtgttt gtatccaaga aagggcttca gaagatcttg aatgtccaga
      481 agaagctgcc gattattcag aagatcatca tcatggacag caaaaccgac taccaaggct
      541 ttcagtccat gtacacgttc gtgacaagcc atctcccccc tgggttcaat gagtacgatt
      601 tcgtgcccga atcttttgat agggacaaaa ccattgccct gattatgaac tcatctggca
      661 gcacaggctt gccgaagggc gtggctctgc cacaccgaac ggcttgcgtt cgctttagtc
      721 acgctcgaga ccctatattc ggtaatcaga tcatacctga cacggccatc ctgtccgttg
      781 tgccatttca ccacggcttt ggaatgttca caacactggg gtacttgatc tgtgggttta
      841 gagtcgtgct catgtaccgg ttcgaggagg aactgtttct gcgcagtctg caggactata
      901 agatacaatc cgccctgttg gtgcccactc tgttctcttt cttcgccaaa tccacactca
      961 tagacaagta cgatcttagc aacctgcatg aaattgcctc cggcggagcc cctctgtcca
     1021 aggaggttgg cgaagctgtg gctaaacggt tccacctgcc tggaatcaga caaggatacg
     1081 ggctcaccga aaccactagc gcaattctga taacacctga gggcgacgac aagccaggag
     1141 cagttggaaa agttgttccg ttctttgagg ctaaggtggt ggacctggac acaggaaaaa
     1201 cactgggtgt gaatcagaga ggcgagctgt gtgttcgcgg acctatgatt atgtcaggat
     1261 atgtgaacaa tcccgaggca acaaacgccc tcatcgataa agatggttgg cttcattccg
     1321 gtgacatagc ctactgggac gaggatgagc acttcttcat cgtcgataga ctgaagtccc
     1381 tgattaaata taagggctat caggttgcgc ctgccgagct ggagtccatt ttgctccaac
     1441 accctaacat ctttgacgca ggtgttgccg ggcttccgga tgatgacgcc ggagagctgc
     1501 cagctgcggt ggttgtgctt gaacatggca agacaatgac tgaaaaagag atcgtggatt
     1561 acgtcgcaag tcaggtgacg accgccaaga agctcagagg tggcgtcgtg ttcgtggatg
     1621 aggttccaaa aggattgacc gggaagctgg atgccagaaa gataagggaa attctgatca
     1681 aggccaagaa gggcggcaaa attgcagtga ctagcacctc ttccggacgc tctagatgac
     1741 acaagcttgc tgttcctggg cgcgccgcgt ggtacctcca cgccaccatg gcctccaaag
     1801 tttacgaccc ggaacagcgc aagcgaatga tcacaggacc ccagtggtgg gcccggtgta
     1861 agcagatgaa tgtactggac agttttatta actactacga tagtgagaaa cacgccgaga
     1921 atgcagtcat ctttctccat ggaaacgccg cctcctctta cttgtggcgg catgtcgtgc
     1981 cccacatcga gccagtcgcg cggtgtatta tcccggacct gatcggaatg ggcaaaagcg
     2041 gaaaatcagg caacggatcc taccgacttc ttgaccacta caagtacctc acggcctggt
     2101 tcgagctgtt gaacctcccc aaaaaaatta ttttcgtggg gcacgactgg ggtgcttgtc
     2161 tggcttttca ctatagctac gaacatcagg acaagattaa agctatcgtc catgcggagt
     2221 ccgtggtcga cgttattgag agctgggacg agtggcctga tattgaagaa gatatagctc
     2281 tcattaaatc tgaagagggg gagaagatgg tgcttgagaa taattttttc gtcgaaacaa
     2341 tgctcccctc caaaattatg cgcaaacttg aacctgagga attcgctgct tacctggaac
     2401 cgttcaagga gaagggggaa gtccgccgcc ccactctttc ctggcccagg gaaatccccc
     2461 tggttaaagg cggcaaaccg gacgtggtcc agatcgtaag aaactataat gcatacctga
     2521 gagccagtga tgatctccca aagatgttca tcgaaagcga ccctggtttc tttagtaacg
     2581 caattgtaga aggggccaag aagttcccca acactgaatt tgtgaaggtc aagggcctgc
     2641 atttctcaca ggaggatgcc cccgatgaga tggggaagta catcaagtct ttcgttgagc
     2701 gggtgctgaa gaacgaacaa tgagcggccg cttcgagcag acatgataag atacattgat
     2761 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
     2821 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat
     2881 tgcattcatt ttatgtttca ggttcagggg gagatgtggg aggtttttta aagcaagtaa
     2941 aacctctaca aatgtggtaa aatcgataag gatccgggct ggcgtaatag cgaagaggcc
     3001 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggac gcgccctgta
     3061 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     3121 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     3181 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     3241 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     3301 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     3361 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     3421 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     3481 acaaaatatt aacgcttaca atttcctgat gcggtatttt ctccttacgc atctgtgcgg
     3541 tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     3601 ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
     3661 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
     3721 gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     3781 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     3841 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     3901 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     3961 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     4021 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     4081 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     4141 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     4201 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     4261 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     4321 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     4381 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     4441 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     4501 gttgcgcaaa ctattaactg gcgaactact tactcttgct tcccggcaac aattaataga
     4561 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     4621 gtttattgct gataaatctg gagccggtga gcgtgggtcg cgcggtatca ttgcagcact
     4681 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     4741 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     4801 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     4861 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     4921 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     4981 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     5041 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     5101 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     5161 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     5221 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     5281 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     5341 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     5401 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     5461 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     5521 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     5581 tttacggttc ctggcctttt gctggccttt tgctcacatg gctcgacaga tctccagatc
     5641 tggcctccgc gccgggtttt ggcgcctccc gcgggcgccc ccctcctcac ggcgagcgct
     5701 gccacgtcag acgaagggcg cagcgagcgt cctgatcctt ccgcccggac gctcaggaca
     5761 gcggcccgct gctcataaga ctcggcctta gaaccccagt atcagcagaa ggacatttta
     5821 ggacgggact tgggtgactc tagggcactg gttttctttc cagagagcgg aacaggcgag
     5881 gaaaagtagt cccttctcgg cgattctgcg gagggatctc cgtggggcgg tgaacgccga
     5941 tgattatata aggacgcgcc gggtgtggca cagctagttc cgtcgcagcc gggatttggg
     6001 tcgcggttct tgtttgtgga tcgctgtgat cgtcacttgg tgagtagcgg gctgctgggc
     6061 tggccggggc tttcgtggcc gccgggccgc tcggtgggac ggaagcgtgt ggagagtccg
     6121 ccaagggctg tagtctgggt ccgcgagcaa ggttgccctg aactgggggt tggggggagc
     6181 gcagcaaaat ggcggctgtt cccgagtctt gaatggaaga cgcttgtgag gcgggctgtg
     6241 aggtcgttga aacaaggtgg ggggcatggt gggcggcaag aacccaaggt cttgaggcct
     6301 tcgctaatgc gggaaagctc ttattcgggt gagatgggct ggggcaccat ctggggaccc
     6361 tgacgtgaag tttgtcactg actggagaac tcggtttgtc gtctgttgcg ggggcggcag
     6421 ttatggcggt gccgttgggc agtgcacccg tacctttggg agcgcgcgcc ctcgtcgtgt
     6481 cgtgacgtca cccgttctgt tggcttataa tgcagggtgg ggccacctgc cggtaggtgt
     6541 gcggtaggct tttctccgtc gcaggacgca gggttcgggc ctagggtagg ctctcctgaa
     6601 tcgacaggcg ccggacctct ggtgagggga gggataagtg aggcgtcagt ttctttggtc
     6661 ggttttatgt acctatcttc ttaagtagct gaagctccgg ttttgaacta tgcgctcggg
     6721 gttggcgagt gtgttttgtg aagtttttta ggcacctttt gaaatgtaat catttgggtc
     6781 aatatgtaat tttcagtgtt agactagtaa attgtccgct aaattctggc cgtttttggc
     6841 ttttttgtta gacgtgtcca ctcccagttc aattacag
//
