LOCUS       sequence-197850-         951 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses SLC30A10-GFP wild-type in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Selyunin AS, Iles LR, Bartholomeusz G, Mukhopadhyay S
  TITLE     Genome-wide siRNA screen identifies UNC50 as a regulator of Shiga 
            toxin 2 trafficking.
  JOURNAL   J Cell Biol. 2017 Oct 2;216(10):3249-3262. doi: 
            10.1083/jcb.201704015. Epub 2017 Sep 7.
  PUBMED    28883040
REFERENCE   2  (bases 1 to 951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1083/jcb.201704015"; journalName: "J Cell Biol"; date: 
            "2017-10-2- 2"; volume: "216"; issue: "10"; pages: "3249-3262"
COMMENT     Sequence Label: SLC30A10-GFP
FEATURES             Location/Qualifiers
     source          1..951
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      160..169
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(166..168,169..171,172..684)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(211..232)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(472..491)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     819..840
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cngnntgang gcctcngtag aagagacgca agagaagtgg ctattgaagt gtctttggat
       61 agctgtctga gtgaccacgg acaaagtctt aacaaaactc aggaggacca atgttatgtc
      121 aacagaacgc attttggtac cgcgggcccg ggatccatcg ccaccatggt gagcaagggc
      181 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
      241 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
      301 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
      361 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
      421 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
      481 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
      541 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
      601 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac
      661 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
      721 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gancacccag
      781 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
      841 accgccgccn gggatcactc tcggcatgga cganctgtac aagtaaagcg gccgcgactc
      901 tagatcataa tcagccatac cacatttgta gaggttttac tngctttaaa a
//
