LOCUS       sequence-197854-       10303 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GFP vector for cloning in guides.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10303)
  AUTHORS   Weintraub AS, Li CH, Zamudio AV, Sigova AA, Hannett NM, Day DS, 
            Abraham BJ, Cohen MA, Nabet B, Buckley DL, Guo YE, Hnisz D, Jaenisch
            R, Bradner JE, Gray NS, Young RA
  TITLE     YY1 Is a Structural Regulator of Enhancer-Promoter Loops.
  JOURNAL   Cell. 2017 Dec 14;171(7):1573-1588.e28. doi: 
            10.1016/j.cell.2017.11.008. Epub 2017 Dec 7.
  PUBMED    29224777
REFERENCE   2  (bases 1 to 10303)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2017.11.008"; journalName: "Cell"; date: 
            "2017-12-14- 14"; volume: "171"; issue: "7"; pages: "1573-1588.e28"
COMMENT     Sequence Label: pAW12.lentiguide.GFP
FEATURES             Location/Qualifiers
     source          1..10303
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        87..1265
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          318..1256
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1213..1233
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1272..1281
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1278..1280,1281..1283,1284..1997)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1278..1280,1281..1283,1284..1994)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1323..1344)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1584..1603)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1931..1952
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2013..2601
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2066..2086)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2484..2495)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2673..2906
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2984..3105
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3021..3040)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3075..3094
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3145..3280
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3300..3319)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3301..3319)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3329..3346)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3329..3345)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3338..3360)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3487..3942
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3574..3593)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3784..3805
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3968..4072
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4073..4141,4142..4933)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4291..4310)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5104..5692
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5593..5612
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5846..5863
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5980..6001
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6016..6021,6022..6039,6040..6046)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6054..6070
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6059..6081
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6078..6094
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6078..6094
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6113..6133
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6115..6133
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6161..6387
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6388..6568
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6615..6740
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7233..7466
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7651..7695
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7873..8121
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     7873..7893
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     8044..8063
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        10003..10078
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    10134..10251
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cccgggtgca aagatggata aagttttaaa cagagaggaa tctttgcagc taatggacct
       61 tctaggtctt gaaaggagtg ggaattggct ccggtgcccg tcagtgggca gagcgcacat
      121 cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgatccggt gcctagagaa
      181 ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg
      241 gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt
      301 ttgccgccag aacacaggta agtgccgtgt gtggttcccg cgggcctggc ctctttacgg
      361 gttatggccc ttgcgtgcct tgaattactt ccacctggct gcagtacgtg attcttgatc
      421 ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt
      481 cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt
      541 ggcaccttcg cgcctgtctc gctgctttcg ataagtctct agccatttaa aatttttgat
      601 gacctgctgc gacgcttttt ttctggcaag atagtcttgt aaatgcgggc caagatctgc
      661 acactggtat ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca
      721 catgttcggc gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc
      781 aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg
      841 cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga aagatggccg cttcccggcc
      901 ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac
      961 ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac tccacggagt
     1021 accgggcgcc gtccaggcac ctcgattagt tctcgagctt ttggagtacg tcgtctttag
     1081 gttgggggga ggggttttat gcgatggagt ttccccacac tgagtgggtg gagactgaag
     1141 ttaggccagc ttggcacttg atgtaattct ccttggaatt tgcccttttt gagtttggat
     1201 cttggttcat tctcaagcct cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt
     1261 cgtgacgtac ggccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
     1321 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
     1381 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
     1441 ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct
     1501 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
     1561 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
     1621 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
     1681 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg
     1741 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg
     1801 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
     1861 tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga
     1921 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
     1981 acgagctgta caagtaaacg cgttaagtcg acaatcaacc tctggattac aaaatttgtg
     2041 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     2101 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     2161 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     2221 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     2281 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     2341 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     2401 cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg
     2461 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     2521 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     2581 ccctttgggc cgcctccccg cgtcgacttt aagaccaatg acttacaagg cagctgtaga
     2641 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
     2701 acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
     2761 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     2821 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     2881 ttagtcagtg tggaaaatct ctagcagtac gtatagtagt tcatgtcatc ttattattca
     2941 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc
     3001 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     3061 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta
     3121 gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     3181 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     3241 gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc
     3301 cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg
     3361 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
     3421 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
     3481 aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     3541 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     3601 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     3661 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     3721 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     3781 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     3841 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     3901 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg
     3961 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     4021 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     4081 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     4141 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     4201 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     4261 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     4321 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     4381 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     4441 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     4501 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     4561 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     4621 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     4681 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     4741 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     4801 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     4861 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     4921 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     4981 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     5041 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     5101 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     5161 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     5221 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     5281 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     5341 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     5401 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     5461 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     5521 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     5581 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     5641 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     5701 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     5761 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     5821 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     5881 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     5941 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     6001 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     6061 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa
     6121 ccctcactaa agggaacaaa agctggagct gcaagcttaa tgtagtctta tgcaatactc
     6181 ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg agagaaaaag
     6241 caccgtgcat gccgattggt ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa
     6301 cagacgggtc tgacatggat tggacgaacc actgaattgc cgcattgcag agatattgta
     6361 tttaagtgcc tagctcgata cataaacggg tctctctggt tagaccagat ctgagcctgg
     6421 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     6481 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     6541 ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag
     6601 ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag
     6661 gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga
     6721 gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa
     6781 ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca
     6841 gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac
     6901 aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat
     6961 ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg
     7021 aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg
     7081 ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat
     7141 aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga
     7201 gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga
     7261 gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta
     7321 ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat
     7381 ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa
     7441 agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc
     7501 accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat
     7561 cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc
     7621 ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat
     7681 aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta
     7741 ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata
     7801 gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg
     7861 aggggaccca gagagggcct atttcccatg attccttcat atttgcatat acgatacaag
     7921 gctgttagag agataattag aattaatttg actgtaaaca caaagatatt agtacaaaat
     7981 acgtgacgta gaaagtaata atttcttggg tagtttgcag ttttaaaatt atgttttaaa
     8041 atggactatc atatgcttac cgtaacttga aagtatttcg atttcttggc tttatatatc
     8101 ttgtggaaag gacgaaacac cggagacggt tgtaaatgag cacacaaaat acacatgcta
     8161 aaatattata ttctatgacc tttataaaat caaccaaaat cttcttttta ataactttag
     8221 tatcaataat tagaattttt atgttccttt ttgcaaactt ttaataaaaa tgagcaaaat
     8281 aaaaaaacgc tagttttagt aactcgcgtt gttttcttca cctttaataa tagctactcc
     8341 accacttgtt cctaagcggt cagctcctgc ttcaatcatt ttttgagcat cttcaaatgt
     8401 tctaactcca ccagctgctt taactaaagc attgtcttta acaactgact tcattagttt
     8461 aacatcttca aatgttgcac ctgattttga aaatcctgtt gatgttttaa caaattctaa
     8521 tccagcttca acagctattt cacaagcttt catgatttct tcttttgtta ataaacaatt
     8581 ttccataata catttaacaa catgtgatcc agctgctttt tttacagctt tcatgtcttc
     8641 taaaactaat tcataatttt tgtcttttaa tgcaccaata tttaatacca tatcaatttc
     8701 tgttgcacca tctttaattg cttcagaaac ttcgaatgct tttgtagctg ttgtgcatgc
     8761 acctagagga aaacctacaa catttgttat tcctacattt gtgcctttta ataattcttt
     8821 acaatagctt gttcaatatg aattaacaca aactgttgca aaatcaaatt caattgcttc
     8881 atcacataat tgtttaattt cagctttcgt agcatcttgt tttaataatg tgtgatctat
     8941 atatttgttt agtttcattt tttctcctat atattcattt ttaattttaa ttctttaata
     9001 atttcgtcta ctttaacttt agcgttttga acagattcac caacacctat aaaataaatt
     9061 tttagtttag gttcagttcc acttgggcga acagcaaatc atgacttatc ttctaaataa
     9121 aattttagta agtcttgtcc tggcatatta tacattccat cgatgtagtc ttcaacatta
     9181 acaactttaa gtccagcaat ttgagttaag ggtgttgctc tcaatgattt cattaatggt
     9241 tcaattttta atttcttttc ttctggttta aaattcaagt ttaaagtgaa agtgtaatat
     9301 gcacccattt ctttaaataa atcttctaaa tagtctacta atgttttatt ttgtttttta
     9361 taaaatcaag cagcctctgc tattaatata gaagcttgta ttccatcttt atctctagct
     9421 gagtcatcaa ttacatatcc ataactttct tcataagcaa aaacaaaatt taatccgtta
     9481 tcttcttctt tagcaatttc tctacccatt catttaaatc cagttaaagt ttttacaata
     9541 ttaactccat atttttcatg agcgattcta tcacccaaat cacttgttac aaaacttgaa
     9601 tatagagccg gattttttgg aatgctattt aagcgtttta gatttgataa ttttcaatca
     9661 attaaaattg gtcctgtttg atttccatct aatcttacaa aatgaccatc atgttttatt
     9721 gccattccaa atctgtcagc atctgggtca ttcataataa taatatctgc atcatgttta
     9781 ataccatatt caagcggtat ttttcatgca ggatcaaatt ctggatttgg atttacaaca
     9841 tttttaaatg tttcatcttc aaatgcatgc tcttcaacct caataacgtt atatcctgat
     9901 tcacgtaata tttttggggt aaatttagtt cctgttccat taactgcgct aaaaataatt
     9961 tttaaatctt ttttagcttc ttgctctttt ttgtacgtct ctgttttaga gctagaaata
    10021 gcaagttaaa ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtgctt
    10081 ttttaagctt ggcgtaacta gatcttgaga caaatggcag tattcatcca caattttaaa
    10141 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
    10201 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat
    10261 tacagggaca gcagagatcc actttggcgc cggctcgagg ggg
//
