LOCUS       sequence-197879-        9227 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses CD9 with mEmerald tag (NOT GFP) at the N terminal.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9227)
  AUTHORS   Boker KO, Lemus-Diaz N, Rinaldi Ferreira R, Schiller L, Schneider S,
            Gruber J
  TITLE     The Impact of the CD9 Tetraspanin on Lentivirus Infectivity and 
            Exosome Secretion.
  JOURNAL   Mol Ther. 2018 Feb 7;26(2):634-647. doi: 
            10.1016/j.ymthe.2017.11.008. Epub 2017 Nov 16.
  PUBMED    29221804
REFERENCE   2  (bases 1 to 9227)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ymthe.2017.11.008"; journalName: "Mol Ther"; date: 
            "2018-02-7- 7"; volume: "26"; issue: "2"; pages: "634-647"
COMMENT     Sequence Label: pLenti6.3/TO/CD9-GFP
FEATURES             Location/Qualifiers
     source          1..9227
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1804..1921
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2247..2450
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2401..2421
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2452..2470
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2473..2491
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2532..2556
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      2603..2612
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2609..2611,2612..2614,2615..3325)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2654..2675)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2915..2934)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3262..3283
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(4085..4109)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4162..4203
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    4227..4818
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4280..4300)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4701..4712)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4833..5149
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5000..5135
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        5197..5244
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5263..5661
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             5748..5981
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6053..6174
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6090..6109)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6144..6163
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6214..6349
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6369..6388)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6370..6388)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6398..6415)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6398..6414)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6407..6429)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6556..7011
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6643..6662)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6853..6874
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7037..7141
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7142..7210,7211..8002)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7360..7379)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8173..8761
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8662..8681
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8915..8932
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9049..9070
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9085..9090,9091..9108,9109..9115)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9123..9139
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9128..9150
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9147..9163
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9147..9163
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9182..9202
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9184..9202
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgataag cttgggagtt ccgcttacat aacttacggt aaatggcccg cctggctgac
     1981 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
     2041 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
     2101 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
     2161 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
     2221 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
     2281 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
     2341 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
     2401 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct ctccctatca
     2461 gtgatagaga tctccctatc agtgatagag atcgtcgact agtccagtgt ggtggaattc
     2521 tgcagatatc aacaagtttg tacaaaaaag caggctttaa aggaaccaat tcagtcgact
     2581 ggatccggta ccgaattcga ttgccaccat ggtgagcaag ggcgaggagc tgttcaccgg
     2641 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
     2701 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
     2761 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ttgacctacg gcgtgcagtg
     2821 cttcgcccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
     2881 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
     2941 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
     3001 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaaggt
     3061 ctatatcacc gccgacaagc agaagaacgg catcaaggtg aacttcaaga cccgccacaa
     3121 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
     3181 cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccaagc tgagcaaaga
     3241 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
     3301 tctcggcatg gacgagctgt acaagtccgg actcagatct cgagctcaag cttccccggt
     3361 caaaggaggc accaagtgca tcaaatacct gctgttcgga tttaacttca tcttctggct
     3421 tgccgggatt gctgtccttg ccattggact atggctccga ttcgactctc agaccaagag
     3481 catctycgag caagaaacta ataataataa ttccagcttc tacacaggag tctatattct
     3541 gatcggagcc ggcgccctca tgatgctggt gggcttcctg ggctgctgcg gggctgtgca
     3601 ggagtcccag tgcatgctgg gactgttctt cggcttcctc ttggtgatat tcgccattga
     3661 aatagctgcg gccatctggg gatattccca caaggatgag gtgattaagg aagtccagga
     3721 gttttacaag gacacctaca acaagctgaa aaccaaggat gagccccagc gggaaacgct
     3781 gaaagccatc cactatgcgt tgaactgctg tggtttggct gggggcgtgg aacagtttat
     3841 ctcagacatc tgccccaaga aggacgtact cgaaaccttc accgtgaagt cctgtcctga
     3901 tgccatcaaa gaggtcttcg acaataaatt ccacatcatc ggcgcagtgg gcatcggcat
     3961 tgccgtggtc atgatatttg gcatgatctt cagtatgatc ttgtgctgtg ctatccgcag
     4021 gaaccgcgag atggtctagg gatccaatca ctagtgaatt cgcggccgca ctcgagatat
     4081 ctagacccag ctttcttgta caaagtggtt gatatccagc acagtggcgg ccgctcgagt
     4141 ctagagggcc cgcggttcga aggtaagcct atccctaacc ctctcctcgg tctcgattct
     4201 acgcgtaccg gttagtaatg atcgacaatc aacctctgga ttacaaaatt tgtgaaagat
     4261 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     4321 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     4381 gggttgctgt ctctttatga aggagttgtg ggcccgttgt caggcaacgt ggcgtggtgt
     4441 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     4501 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     4561 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     4621 ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     4681 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     4741 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     4801 tttgggccgc ctccccgcct ggcgatggta ccggtgtgga aagtccccag gctccccagc
     4861 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     4921 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     4981 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     5041 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct
     5101 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg
     5161 agcttgtata tccattttcg gatctgatca gcacgtgttg acaattaatc atcggcatag
     5221 tatatcggca tagtataata cgacaaggtg aggaactaaa ccatggccaa gcctttgtct
     5281 caagaagaat ccaccctcat tgaaagagca acggctacaa tcaacagcat ccccatctct
     5341 gaagactaca gcgtcgccag cgcagctctc tctagcgacg gccgcatctt cactggtgtc
     5401 aatgtatatc attttactgg gggaccttgt gcagaactcg tggtgctggg cactgctgct
     5461 gctgcggcag ctggcaacct gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc
     5521 ttgagcccct gcggacggtg ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc
     5581 atagtgaagg acagtgatgg acagccgacg gcagttggga ttcgtgaatt gctgccctct
     5641 ggttatgtgt gggagggcta agcacaattc gagctcggta cctttaagac caatgactta
     5701 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     5761 tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca
     5821 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     5881 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     5941 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct
     6001 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt
     6061 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     6121 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     6181 ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     6241 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     6301 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct agggacgtac
     6361 ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc
     6421 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
     6481 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
     6541 tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     6601 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     6661 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     6721 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     6781 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     6841 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     6901 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     6961 tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac
     7021 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     7081 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     7141 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     7201 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     7261 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     7321 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     7381 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     7441 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     7501 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     7561 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     7621 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     7681 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     7741 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     7801 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     7861 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     7921 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     7981 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     8041 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     8101 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     8161 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     8221 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     8281 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     8341 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     8401 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     8461 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     8521 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     8581 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     8641 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     8701 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     8761 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     8821 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     8881 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     8941 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     9001 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
     9061 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
     9121 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg
     9181 cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagctt
//
