LOCUS       sequence-197913-        5624 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses c-Myc in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5624)
  AUTHORS   Jing H, Hu J, He B, Negron Abril YL, Stupinski J, Weiser K, 
            Carbonaro M, Chiang YL, Southard T, Giannakakou P, Weiss RS, Lin H
  TITLE     A SIRT2-Selective Inhibitor Promotes c-Myc Oncoprotein Degradation 
            and Exhibits Broad Anticancer Activity.
  JOURNAL   Cancer Cell. 2016 Mar 14;29(3):297-310. doi: 
            10.1016/j.ccell.2016.02.007.
  PUBMED    26977881
REFERENCE   2  (bases 1 to 5624)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.02"; journalName: "Cancer Cell"; date: 
            "2016-03-14- 14"; volume: "29"; issue: "3"; pages: "297-310"
COMMENT     Sequence Label: pCMV4a-Flag-c-Myc
FEATURES             Location/Qualifiers
     source          1..5624
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        43..346
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        347..550
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     501..521
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        596..614
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             672..695
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             699..2018
                     /codon_start=1
                     /product="human c-Myc proto-oncogene"
                     /label=c-Myc
                     /translation="MPLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQQSELQPPAP
                     SEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFSTADQLEMVTE
                     LLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSGSPN
                     PARGHSVCSTSSLYLQDLSAAASECIDPSVVFPYPLNDSSSPKSCASQDSSAFSPSSDS
                     LLSSTESSPQGSPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQAPGKRSESGS
                     PSAGGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRVKLDSVRVLRQISNNR
                     KCTSPRSSDTEENVKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKKA
                     TAYILSVQAEEQKLISEEDLLRKRREQLKHKLEQLRNSCA"
     CDS             2025..2048
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(2101..2120)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2102..2120)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    2394..2515
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2431..2450)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2485..2504
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2522..2977)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2659..2680)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2871..2890
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3004..3108
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3110..3467
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3110..3301
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3133..3153)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3318..3453
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3502..4296
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3502..4296
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3556..3575)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4166..4185
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4484..4503)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4528..4575
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4904..5492
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5393..5412
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
       61 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
      121 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
      181 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
      241 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
      301 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
      361 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
      421 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
      481 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
      541 aagcagagct ggtttagtga accgtcagat ccgctagcga ttacgccaag ctcgaaatta
      601 accctcacta aagggaacaa aagctggagc tccaccgcgg tggcggccgc tctagcccgg
      661 gcggatccat ggattacaag gatgacgacg ataagggaat gcccctcaac gttagcttca
      721 ccaacaggaa ctatgacctc gactacgact cggtgcagcc gtatttctac tgcgacgagg
      781 aggagaactt ctaccagcag cagcagcaga gcgagctgca gcccccggcg cccagcgagg
      841 atatctggaa gaaattcgag ctgctgccca ccccgcccct gtcccctagc cgccgctccg
      901 ggctctgctc gccctcctac gttgcggtca cacccttctc ccttcgggga gacaacgacg
      961 gcggtggcgg gagcttctcc acggccgacc agctggagat ggtgaccgag ctgctgggag
     1021 gagacatggt gaaccagagt ttcatctgcg acccggacga cgagaccttc atcaaaaaca
     1081 tcatcatcca ggactgtatg tggagcggct tctcggccgc cgccaagctc gtctcagaga
     1141 agctggcctc ctaccaggct gcgcgcaaag acagcggcag cccgaacccc gcccgcggcc
     1201 acagcgtctg ctccacctcc agcttgtacc tgcaggatct gagcgccgcc gcctcagagt
     1261 gcatcgaccc ctcggtggtc ttcccctacc ctctcaacga cagcagctcg cccaagtcct
     1321 gcgcctcgca agactccagc gccttctctc cgtcctcgga ttctctgctc tcctcgacgg
     1381 agtcctcccc gcagggcagc cccgagcccc tggtgctcca tgaggagaca ccgcccacca
     1441 ccagcagcga ctctgaggag gaacaagaag atgaggaaga aatcgatgtt gtttctgtgg
     1501 aaaagaggca ggctcctggc aaaaggtcag agtctggatc accttctgct ggaggccaca
     1561 gcaaacctcc tcacagccca ctggtcctca agaggtgcca cgtctccaca catcagcaca
     1621 actacgcagc gcctccctcc actcggaagg actatcctgc tgccaagagg gtcaagttgg
     1681 acagtgtcag agtcctgaga cagatcagca acaaccgaaa atgcaccagc cccaggtcct
     1741 cggacaccga ggagaatgtc aagaggcgaa cacacaacgt cttggagcgc cagaggagga
     1801 acgagctaaa acggagcttt tttgccctgc gtgaccagat cccggagttg gaaaacaatg
     1861 aaaaggcccc caaggtagtt atccttaaaa aagccacagc atacatcctg tccgtccaag
     1921 cagaggagca aaagctcatt tctgaagagg acttgttgcg gaaacgacga gaacagttga
     1981 aacacaaact tgaacagcta cggaactctt gtgcgtaact cgaggattac aaggatgacg
     2041 acgataagta gggcccggta ccttaattaa ttaaggtacc aggtaagtgt acccaattcg
     2101 ccctatagtg agtcgtatta caattcactc gatcgccctt cccaacagtt gcgcagcctg
     2161 aatggcgaat ggagatccaa tttttaagtg tataatgtgt taaactactg attctaattg
     2221 tttgtgtatt ttagattcac agtcccaagg ctcatttcag gcccctcagt cctcacagtc
     2281 tgttcatgat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc
     2341 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
     2401 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     2461 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttaacgcg
     2521 taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca
     2581 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag
     2641 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc
     2701 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc
     2761 taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc
     2821 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa
     2881 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc
     2941 acacccgccg cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt tcggggaaat
     3001 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     3061 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagaatcc tgaggcggaa
     3121 agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
     3181 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     3241 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     3301 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     3361 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     3421 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaagat cgatcaagag
     3481 acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc
     3541 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat
     3601 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg
     3661 tccggtgccc tgaatgaact gcaagacgag gcagcgcggc tatcgtggct ggccacgacg
     3721 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta
     3781 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta
     3841 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
     3901 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc
     3961 gatcaggatg atctggacga agaacatcag gggctcgcgc cagccgaact gttcgccagg
     4021 ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg
     4081 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt
     4141 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agaacttggc
     4201 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc
     4261 atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga
     4321 ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg
     4381 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg
     4441 atctcatgct ggagttcttc gcccacccta gggggaggct aactgaaaca cggaaggaga
     4501 caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa acgcacggtg
     4561 ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg ctggcactct gtcgataccc
     4621 caccgagacc ccattggggc caatacgccc gcgtttcttc cttttcccca ccccaccccc
     4681 caagttcggg tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag
     4741 cctcaggtta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     4801 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     4861 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     4921 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     4981 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     5041 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     5101 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     5161 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     5221 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     5281 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     5341 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     5401 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     5461 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     5521 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     5581 tggataaccg tattaccgcc atgcattagt tattaatagt aatc
//
