LOCUS       sequence-197931-        7678 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of Coronin1 in eukaryotic cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7678)
  AUTHORS   Gatfield J, Albrecht I, Zanolari B, Steinmetz MO, Pieters J
  TITLE     Association of the leukocyte plasma membrane with the actin 
            cytoskeleton through coiled coil-mediated trimeric coronin 1 
            molecules.
  JOURNAL   Mol Biol Cell. 2005 Jun;16(6):2786-98. Epub 2005 Mar 30.
  PUBMED    15800061
REFERENCE   2  (bases 1 to 7678)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell."; date: "2005-06"; volume: "16(6)"; pages: "2786-98. Epub 2005
            Mar 30"
COMMENT     Sequence Label: pCB6-Cor1-HA
FEATURES             Location/Qualifiers
     source          1..7678
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     175..195
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     polyA_signal    219..841
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     623..642
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     CDS             complement(888..914)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(2377..2580)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(2406..2426)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        2581..2960
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(3121..3138)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3121..3137)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3130..3152)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(3278..3733)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3415..3436)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3627..3646
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3760..3864
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3865..3933,3934..4725)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4083..4102)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4896..5484
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5385..5404
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5638..5655
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5772..5793
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5808..5813,5814..5831,5832..5838)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5846..5862
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5851..5873
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5870..5886
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5870..5886
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5900..5919
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5900..5918
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    5924..6058
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5948..5967)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6002..6021
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(6710..7504)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6821..6840)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     7431..7450
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(join(7558..7678,1..177))
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
ORIGIN
        1 ggggcgggat gggcggagtt aggggcggga ctatggttgc tgactaattg agatgcatgc
       61 tttgcatact tctgcctgct ggggagcctg gggactttcc acacctggtt gctgactaat
      121 tgagatgcat gctttgcata cttctgcctg ctggggagcc tggggacttt ccacacccta
      181 actgacacac attccacagc caagctggcc gcgtacccaa ttcaacaggc atctactgag
      241 tggacccaac gcatgagagg acagtgccaa gcaagcaact caaatgtccc accggttggg
      301 catggccagg tagcctatgc tgtgtctgga cgtcctcctg ctggtatagt tattttaaaa
      361 tcagaaggac agggaaggga gcagtggttc acgcctgtaa tcccagcaat ttgggaggcc
      421 aaggtgggta gatcacctga gattaggagt tggagaccag cctggccaat atggtgaaac
      481 cccgtctcta ccaaaaaaac aaaaattagc tgagcctggt catgcatgcc tggaatccca
      541 acaactcggg aggctgaggc aggagaatcg cttgaaccca ggaggcggag attgcagtga
      601 gccaagattg tgccactgca ctccagcttg gttcccaata gaccccgcag gccctacagg
      661 ttgtcttccc aacttgcccc ttgctccata ccacccccct ccaccccata atattataga
      721 aggacaccta gtcagacaaa atgatgcaac ttaattttat taggacaagg ctggtgggca
      781 ctggagtggc aacttccagg gccaggagag gcactgggga ggggtcacag ggatgccacc
      841 cgggatcctc tagagtcgac ctgcaggcat gcaagctgga attcctatgc gtagtctggt
      901 acgtcgtatg ggtacttggc ctgaacagtc tcctccagcc tgtccaggcg ctcttgtagc
      961 ttttgcacta tggcgttcag attcctcacg tcctcctcca gccttgacac ggtatccgag
     1021 ctgggcgtgc cgctgggctc tggtgtagct cttctgcgag cgctgtccag gcccctgttg
     1081 accctcagct ccctgctctt tgggggcacg tagccatcct tgagggaaat gaggaggggg
     1141 ccagcatccc gaccacccag ccattcctca gctgtgaggg cagggtcggg tcctgcagtg
     1201 ggcggataca ggtcctcctg gaacaggtcc gactttctag gcactgtcat ggcaatgggc
     1261 tcacacttcc gctcatgaag cttatagaat ctggcaatct cacacttatt cacttccagg
     1321 ccacgtttgg gcatgtagcc cataccacgc tgagactcct tggaactgaa catggaaaga
     1381 taatgcagga acggagcctc agaagtgatc tcaaaatacc ggatagagct gtcacccttg
     1441 ccacagaggt agacaatgtt ggtatcagga tcaaaaaaag gtagcaggac tccgctgctt
     1501 gtgtccagct cctgcaggga cagtggctcc tccaggtgct ttgtgtccca cagcgccacc
     1561 tgccgctcac tcatgcgact gaaacccgtg gttagaatct tcccttctga cacaaacaca
     1621 gcgtgcactg gccgagtccc ctcatgggga cggtcctttt cagccacaac agtgcctttg
     1681 cgaggctcga tgacacgaac tcgcttgtca cggcaggagg tgcagatgag agcaccgtcc
     1741 cggctccagt ccacgctgta gattgtgtcc gggtgcacat ccgggcccag cgtcagcaca
     1801 gctgccccag tgcccacgtc ccacaccagg atcacattgt cacaacctgc gctgagaagc
     1861 acattctggg ctgtggggtg ccaggccacg atgccaaccc tcttggtgtg gccctccaag
     1921 gtgatgacag gctcccgcaa gggcagcacc aggcccccat ccgggatctc ccacaccata
     1981 actgtgcagt cctcagagcc actggcaatg acattgtcat tgtgtggaca ccaggcgatg
     2041 tctagcacag gggcagtgtg gccgcagacc aggggcacgt tcttgtctac tcgtccagtc
     2101 ttgcctaggg gtagcaccag gaaggcccct cccccactgg cctcacagat cagagccatg
     2161 aacttggggt tgacagcgca gaagccactg tcccaagtgg tttgtgagac gcgcacatcc
     2221 tcatagcact ggtcagcctt ggctggctgt ccaaacacgt ggcggaattt gctggagcga
     2281 accacctgcc ggctcatcct gctgcctcgt gccgaattcc agcttatcga tacgcgtggt
     2341 accagatctg aattaattct gacggttcac taaacgagct ctgcttatat agacctccca
     2401 ccgtacacgc ctaccgccca tttgcgtcaa cggggcgggg ttattacgac attttggaaa
     2461 gtcccgttga ttttggtgcc aaaacaaact cccattgacg tcaatggggt ggagacttgg
     2521 aaatccccgt gagtcaaacc gctatccacg cccattggtg tactgccaaa accgcatcac
     2581 catggtaata gcgatgacta atacgtagat gtactgccaa gtaggaaagt cccgtaaggt
     2641 catgtactgg gcataatgcc aggcgggcca tttaccgtca ttgacgtcaa tagggggcgg
     2701 acttggcata tgatacactt gatgtactgc caagtgggca gtttaccgta aatactccac
     2761 ccattgacgt caatggaaag tccctattgg cgttactatg ggaacatacg tcattattga
     2821 cgtcaatggg cgggggtcgt tgggcggtca gccaggcggg ccatttaccg taagttatgt
     2881 aacgcggaac tccatatatg ggctatgaac taatgacccc gtaattgatt actattaata
     2941 actagtcaat aatcaatgtc aacatggcgg tcatattgga catgagccaa tataaatgta
     3001 catattatga tatagataca acgtatgcaa tggccaatag ccaatattga tttatgctat
     3061 ataaccaatg actaatatgg ctaattgcca atattgattc aatgtataga tcagcttggc
     3121 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
     3181 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
     3241 cccttcccaa cagttgcgca gcctgaatgg cgaatggaaa ttgtaagcgt taatattttg
     3301 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc
     3361 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt
     3421 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc
     3481 tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg
     3541 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga
     3601 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg
     3661 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg
     3721 ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     3781 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     3841 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     3901 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     3961 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     4021 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     4081 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     4141 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     4201 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     4261 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     4321 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     4381 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     4441 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     4501 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     4561 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     4621 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     4681 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     4741 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     4801 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     4861 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     4921 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     4981 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     5041 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     5101 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     5161 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     5221 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     5281 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     5341 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     5401 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     5461 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     5521 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     5581 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     5641 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     5701 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     5761 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
     5821 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
     5881 tatgacatga ttacgaattt aatacgactc actataggga attgatccag acatgataag
     5941 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg
     6001 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
     6061 caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta
     6121 aagcaagtaa aacctctaca aatgtggtat ggctgattat gatcggcgga gcctatggaa
     6181 aaacgccagc aacgcggcct ttttacggtt ccagcttggc gagtgggtgg tgagcagctc
     6241 gccgtcgcgc agcaggcgcc agcgctccat ccacgcagtg acctcaggcg gaaaacggga
     6301 agacacactc atgagatgcc tgcaagcaat tcgttctgta tcaggcgcag gagcgtcccg
     6361 tccgggtcga ccaaagcggc catcgtgcct ccccactcct gcagttcggg ggcatggatg
     6421 cgcggatagc cgctgctggt ttcctggatg ccgacggatt tgcactgccg gtagaactcc
     6481 gcgaggtcgt ccagcctcag gcagcagctg aaccaactcg cgaggggatc gagcccgggg
     6541 tgggcgaaga actccagcat gagatccccg cgctggagga tcatccagcc ggcgtcccgg
     6601 aaaacgattc cgaagcccaa cctttcatag aaggcggcgg tggaatcgaa atctcgtgat
     6661 ggcaggttgg gcgtcgcttg gtcggtcatt tcgaacccca gagtcccgct cagaagaact
     6721 cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg agcggcgata ccgtaaagca
     6781 cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc aatatcacgg gtagccaacg
     6841 ctatgtcctg atagcggtcc gccacaccca gccggccaca gtcgatgaat ccagaaaagc
     6901 ggccattttc caccatgata ttcggcaagc aggcatcgcc atgggtcacg acgagatcct
     6961 cgccgtcggg catgcgcgcc ttgagcctgg cgaacagttc ggctggcgcg agcccctgat
     7021 gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc catccgagta cgtgctcgct
     7081 cgatgcgatg tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc gtatgcagcc
     7141 gccgcattgc atcagccatg atggatactt tctcggcagg agcaaggtga gatgacagga
     7201 gatcctgccc cggcacttcg cccaatagca gccagtccct tcccgcttca gtgacaacgt
     7261 cgagcacagc tgcgcaagga acgcccgtcg tggccagcca cgatagccgc gctgcctcgt
     7321 cctgcagttc attcagggca ccggacaggt cggtcttgac aaaaagaacc gggcgcccct
     7381 gcgctgacag ccggaacacg gcggcatcag agcagccgat tgtctgttgt gcccagtcat
     7441 agccgaatag cctctccacc caagcggccg gagaacctgc gtgcaatcca tcttgttcaa
     7501 tcatgcgaaa cgatcctcat cctgtctctt gatcagatcc caagctgatc aattcctcca
     7561 aaaaagcctc ctcactactt ctggaatagc tcagaggccg aggcggcctc ggcctctgca
     7621 taaataaaaa aaattagtca gccatggggc ggagaatggg cggaactggg cggagtta
//
