LOCUS       sequence-197948-        3225 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Autophagy cargo assay.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3225)
  AUTHORS   Mercer CA, Kaliappan A, Dennis PB
  TITLE     Macroautophagy-dependent, intralysosomal cleavage of a betaine 
            homocysteine methyltransferase fusion protein requires stable 
            multimerization.
  JOURNAL   Autophagy. 2008 Feb;4(2):185-94. Epub 2007 Nov 7.
  PUBMED    18059170
REFERENCE   2  (bases 1 to 3225)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Autophagy."; date: "2008-02"; volume: "4(2)"; pages: "185-94. Epub 
            2007 Nov 7"
COMMENT     Sequence Label: GST-BHMT-I/G
FEATURES             Location/Qualifiers
     source          1..3225
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     624..646
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     misc_feature    1887..2458
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2054..2071)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2281..2300
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(2461..2463,2464..2466,2467..3177)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2461..2463,2464..2466,2467..3177)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2506..2527)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2767..2786)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3114..3135
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3187..3216
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 cgcgagccag ccatgtcccc tatactaggt tattggaaaa ttaagggcct tgtgcaaccc
       61 actcgacttc ttttggaata tcttgaagaa aaatatgaag agcatttgta tgagcgcgat
      121 gaaggtgata aatggcgagc caaaaagttt gaattgggtt tggagtttcc caatcttcct
      181 tattatattg atggtgatgt taaattaaca cagtctatgg ccatcatacg ttatatagct
      241 gacaagcaca acatgttggg tggttgtcca aaagagcgtg cagagatttc aatgcttgaa
      301 ggagcggttt tggatattag atacggtgtt tcgagaattg catatagtaa agactttgaa
      361 actctcaaag ttgattttct tagcaagcta cctgaaatgc tgaaaatgtt cgaagatcgt
      421 ttatgtcata aaacatattt aaatggtgat catgtaaccc atcctgactt catgttgtat
      481 gacgctcttg atgttgtttt atacatggac ccaatgtgcc tggatgcgtt cccaaaatta
      541 gtttgtttta aaaaacgtat tgaagctatc ccacaaattg ataagtactt gaaatccagc
      601 aagtatatag catggccttt gcagggctgg caagccacgt ttggtggtgg cggccaccca
      661 cctcaatccg gtggcggccg cgccaagaag ggcatcctag aacgtttaaa tgctggagag
      721 attgtgattg gagatggagg gtttgtcttt gcactggaga agaggggcta cgtaaaggca
      781 ggaccctgga ctcctgaagc tgctgtggag cacccagaag cagttcgcca gcttcatcga
      841 gagttcctca gagctggctc aaacgtcatg cagaccttca ccttctatgc gagtgaagac
      901 aagctggaga acaggggcaa ctatgtctta gagaagatat ctgggcagga agtcaatgaa
      961 gctgcttgcg acatcgcccg acaagtggct gatgaaggag atgctttggt agcaggagga
     1021 gtgagtcaga caccttcata ccttagctgc aagagtgaaa ctgaagtcaa aaaagtattt
     1081 ctgcaacagt tagaggtctt tatgaagaag aacgtggact tcttgattgc agagtatttt
     1141 gaacacgttg aagaagctgt gtgggcagtt gaaaccttga tagcatccgg taaacctgtg
     1201 gcagcaacca tgtgcattgg cccagaagga gatttgcatg gcgtgccccc cggcgagtgt
     1261 gcagtgcgcc tggtgaaagc aggagcatcc atcattggtg tgaactgcca ctttgacccc
     1321 accattagtt taaaaacagt gaagctcatg aaggagggct tggaggctgc ccgactgaaa
     1381 gctcacctga tgagccagcc cttggcttac cacactcctg actgcaacaa gcagggattc
     1441 atcgatctcc cagaattccc atttggactg gaacccagag ttgccaccag atgggatatt
     1501 caaaaatacg ccagagaggc ctacaacctg ggggtcaggt acattggcgg gtgctgtgga
     1561 tttgagccct accacatcag ggcaattgca gaggagctgg ccccagaaag gggctttttg
     1621 ccaccagctt cagaaaaaca tggcagctgg ggaagtggtt tggacatgca caccaaaccc
     1681 tgggttagag caagggccag gaaggaatac tgggagaatc ttcggatagc ctcaggccgg
     1741 ccatacaacc cttcaatgtc aaagccagat ggctggggag tgaccaaagg aacagccgag
     1801 ctgatgcagc agaaagaagc cacaactgag cagcagctga aagagctctc tagataagaa
     1861 ttcgttaacc tcgaggatca attccgcccc tctccctccc ccccccctaa cgttactggc
     1921 cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg
     1981 ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct
     2041 aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca
     2101 gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg
     2161 aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct
     2221 gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa
     2281 tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt
     2341 atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa
     2401 aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgataatacc
     2461 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
     2521 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
     2581 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
     2641 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
     2701 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
     2761 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     2821 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     2881 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
     2941 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
     3001 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
     3061 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
     3121 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtct
     3181 agaatggagc agaagctgat ctccgaggag gacctgtgaa agctt
//
