LOCUS       sequence-19797-e         697 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Wright DA, Thibodeau-Beganny S, Sander JD, Winfrey RJ, Hirsh AS, 
            Eichtinger M, Fu F, Porteus MH, Dobbs D, Voytas DF, Joung JK
  TITLE     Standardized reagents and protocols for engineering zinc finger 
            nucleases by modular assembly.
  JOURNAL   Nat Protoc. 2006;1(3):1637-52. doi: 10.1038/nprot.2006.259
  PUBMED    17406455
REFERENCE   2  (bases 1 to 697)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Protoc."; date: "2006"; volume: "1(3):1637-52. doi"; pages: " 
            10.1038/nprot.2006.25"
COMMENT     Sequence Label: pGP-FB-orig BA
FEATURES             Location/Qualifiers
     source          1..697
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(62..67,68..85,86..92)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    100..116
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     105..127
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             418..441
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 agctcacgat acgggttact gatgatgaac atgcccggtt actggaacgg gaattccggg
       61 ctttacactt tatgcttccg gctcgtataa tgtgtcgact tgtgagcgga taacaatttc
      121 acacaggaaa cagctatgcc tcaacagcag caaatgcaac ctcccaattc aagtgcggac
      181 aacaaccctt tgcaacagca atcatcacaa aataccgtac caaacgtcct caaccaaatt
      241 aaccaaatct tttctccaga ggagcaacgc agcttattac aagaagccat cgaaacctgc
      301 aagaattttg aaaaaacaca attgggtagt acgatgacgg aacctgtcaa gcaaagtttt
      361 attaggaaat acattgtgca aaaggccctg agaaaaatcc aagctttggc ggccgctgac
      421 tacaaagacg atgatgacaa atctagaccc ggggagcgcc ccttccagtg tcgcatttgc
      481 atgcggaact tttcggaccg atcctcgctt acgcgtcata cccgtactca taccggtgaa
      541 aaaccgtttc agtgtcggat ctgtatgcga aatttctccc agggggggga atttggtgcg
      601 gcatctacgt acgcacaccg gcgagaagcc attccaatgc cgaatatgca tgcgcaactt
      661 cagtcaggca gccaccctgc agcgacacct aaaaacc
//
