LOCUS       sequence-19800-e         747 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Wright DA, Thibodeau-Beganny S, Sander JD, Winfrey RJ, Hirsh AS, 
            Eichtinger M, Fu F, Porteus MH, Dobbs D, Voytas DF, Joung JK
  TITLE     Standardized reagents and protocols for engineering zinc finger 
            nucleases by modular assembly.
  JOURNAL   Nat Protoc. 2006;1(3):1637-52. doi: 10.1038/nprot.2006.259
  PUBMED    17406455
REFERENCE   2  (bases 1 to 747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Protoc."; date: "2006"; volume: "1(3):1637-52. doi"; pages: " 
            10.1038/nprot.2006.25"
COMMENT     Sequence Label: pBAC-BA-lacZ
FEATURES             Location/Qualifiers
     source          1..747
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(29..45)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(29..45)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        complement(join(74..80,81..98,99..104))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     complement(175..193)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(271..298)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(390..476)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 tggctgcagg tcgaaattcg taatcatggt catagctgtt tcctgtgcga aattgttatc
       61 cgctcggtcg acacaacata cgagccggaa gcataaagtg taaagcccgg ggtgcctaat
      121 gtaatgggct tctgcgtgtc gaagatcttc cacagaattc ttgaagacga aagggcctcg
      181 tgatacgcct atttttatag gttaatgtca tgagcggata catatttgaa tgtatttaga
      241 aaaatcaaca aaagagtttg tagaaacgca aaaaggccat ccgtcaggat ggccttctgc
      301 ttaatttgat gcctggcagt ttatggcggg cgtcctgccc gccaccctcc gggccgttgc
      361 ttctcaacgt tcaaatccgc tcccggcgga tttgtcctac tcaggagagc gttcaccgac
      421 taacaacaga taaaacgaaa ggcccagact ttcgactgag cctttcgttt tatttgatgc
      481 ctggcagttc cctactctcg catggttgcg gccgcccggg ccgtcgacca attctcatgt
      541 ttgacagctt atcatcgaat ttctgccatt catccgctta ttatcactta ttcaggcgta
      601 gcagccaggc gttttaaggg caccaataac tgccttaaaa aaattacgcc ccgccctgtc
      661 actcatcgca gtactgttgt aattcattaa gcattctgcc gacatggaag ccctcacaaa
      721 cgggatgatg aacctgattc gccagcg
//
