LOCUS       sequence-198016-       10073 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression plasmid for SpCas9 Cluster 3 + Q926A variant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10073)
  AUTHORS   Chen JS, Dagdas YS, Kleinstiver BP, Welch MM, Sousa AA, Harrington 
            LB, Sternberg SH, Joung JK, Yildiz A, Doudna JA
  TITLE     Enhanced proofreading governs CRISPR-Cas9 targeting accuracy.
  JOURNAL   Nature. 2017 Sep 20. doi: 10.1038/nature24268.
  PUBMED    28931002
REFERENCE   2  (bases 1 to 10073)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2017 Sep 20. doi: 10.1038/nature24268."
COMMENT     Sequence Label: pJSC239 - Bacterial expression plasmid for SpC...
FEATURES             Location/Qualifiers
     source          1..10073
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1511..1530
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     1614..1633
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1614..1632
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             1721..1743
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1763..1792
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             1802..2902
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     primer_bind     2876..2899
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             2957..2977
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             2984..7090
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYAAAGALSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRAITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     primer_bind     complement(7216..7234)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      7230..7277
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(7643..7648,7649..7665,7666..7671))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(7698..7716)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7784..7888
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7889..7957,7958..8749)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8107..8126)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8920..9508
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9409..9428
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9662..9679
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9694..9833
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9759..9778)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9919..9941
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(join(9935..10073,1..53))
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc
       61 tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg
      121 ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa
      181 caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc
      241 ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc
      301 cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa
      361 ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac
      421 gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc
      481 cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc
      541 gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg
      601 ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg
      661 aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac
      721 cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc
      781 cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccaatgatcg
      841 aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta
      901 cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa
      961 tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg
     1021 cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac
     1081 cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga
     1141 tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca
     1201 cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc
     1261 cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct
     1321 ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca
     1381 ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg
     1441 ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat
     1501 cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga
     1561 tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aattaatacg
     1621 actcactata gggagaccac aacggtttcc ctctagtgcc ggctccggag agctctttaa
     1681 ttaagcggcc gccctgcagg actcgagttc tagaaataat tttgtttaac tttaagaagg
     1741 agatatacat atgaaatctt ctcaccatca ccatcaccat caccatcacc atggttcttc
     1801 tatgaaaatc gaagaaggta aactggtaat ctggattaac ggcgataaag gctataacgg
     1861 tctcgctgaa gtcggtaaga aattcgagaa agataccgga attaaagtca ccgttgagca
     1921 tccggataaa ctggaagaga aattcccaca ggttgcggca actggcgatg gccctgacat
     1981 tatcttctgg gcacacgacc gctttggtgg ctacgctcaa tctggcctgt tggctgaaat
     2041 caccccggac aaagcgttcc aggacaagct gtatccgttt acctgggatg ccgtacgtta
     2101 caacggcaag ctgattgctt acccgatcgc tgttgaagcg ttatcgctga tttataacaa
     2161 agatctgctg ccgaacccgc caaaaacctg ggaagagatc ccggcgctgg ataaagaact
     2221 gaaagcgaaa ggtaagagcg cgctgatgtt caacctgcaa gaaccgtact tcacctggcc
     2281 gctgattgct gctgacgggg gttatgcgtt caagtatgaa aacggcaagt acgacattaa
     2341 agacgtgggc gtggataacg ctggcgcgaa agcgggtctg accttcctgg ttgacctgat
     2401 taaaaacaaa cacatgaatg cagacaccga ttactccatc gcagaagctg cctttaataa
     2461 aggcgaaaca gcgatgacca tcaacggccc gtgggcatgg tccaacatcg acaccagcaa
     2521 agtgaattat ggtgtaacgg tactgccgac cttcaagggt caaccatcca aaccgttcgt
     2581 tggcgtgctg agcgcaggta ttaacgccgc cagtccgaac aaagagctgg caaaagagtt
     2641 cctcgaaaac tatctgctga ctgatgaagg tctggaagcg gttaataaag acaaaccgct
     2701 gggtgccgta gcgctgaagt cttacgagga agagttggcg aaagatccac gtattgccgc
     2761 cactatggaa aacgcccaga aaggtgaaat catgccgaac atcccgcaga tgtccgcttt
     2821 ctggtatgcc gtgcgtactg cggtgatcaa cgccgccagc ggtcgtcaga ctgtcgatga
     2881 agccctgaaa gacgcgcaga ctaattcgag ctcgaacaac aacaacaata acaataacaa
     2941 caacctcggg atcgaggaaa acctgtactt ccaatccaat gcaatggata agaaatactc
     3001 aataggctta gatatcggca caaatagcgt cggatgggcg gtgatcactg atgaatataa
     3061 ggttccgtct aaaaagttca aggttctggg aaatacagac cgccacagta tcaaaaaaaa
     3121 tcttataggg gctcttttat ttgacagtgg agagacagcg gaagcgactc gtctcaaacg
     3181 gacagctcgt agaaggtata cacgtcggaa gaatcgtatt tgttatctac aggagatttt
     3241 ttcaaatgag atggcgaaag tagatgatag tttctttcat cgacttgaag agtctttttt
     3301 ggtggaagaa gacaagaagc atgaacgtca tcctattttt ggaaatatag tagatgaagt
     3361 tgcttatcat gagaaatatc caactatcta tcatctgcga aaaaaattgg tagattctac
     3421 tgataaagcg gatttgcgct taatctattt ggccttagcg catatgatta agtttcgtgg
     3481 tcattttttg attgagggag atttaaatcc tgataatagt gatgtggaca aactatttat
     3541 ccagttggta caaacctaca atcaattatt tgaagaaaac cctattaacg caagtggagt
     3601 agatgctaaa gcgattcttt ctgcacgatt gagtaaatca agacgattag aaaatctcat
     3661 tgctcagctc cccggtgaga agaaaaatgg cttatttggg aatctcattg ctttgtcatt
     3721 gggtttgacc cctaatttta aatcaaattt tgatttggca gaagatgcta aattacagct
     3781 ttcaaaagat acttacgatg atgatttaga taatttattg gcgcaaattg gagatcaata
     3841 tgctgatttg tttttggcag ctaagaattt atcagatgct attttacttt cagatatcct
     3901 aagagtaaat actgaaataa ctaaggctcc cctatcagct tcaatgatta aacgctacga
     3961 tgaacatcat caagacttga ctcttttaaa agctttagtt cgacaacaac ttccagaaaa
     4021 gtataaagaa atcttttttg atcaatcaaa aaacggatat gcaggttata ttgatggggg
     4081 agctagccaa gaagaatttt ataaatttat caaaccaatt ttagaaaaaa tggatggtac
     4141 tgaggaatta ttggtgaaac taaatcgtga agatttgctg cgcaagcaac ggacctttga
     4201 caacggctct attccccatc aaattcactt gggtgagctg catgctattt tgagaagaca
     4261 agaagacttt tatccatttt taaaagacaa tcgtgagaag attgaaaaaa tcttgacttt
     4321 tcgaattcct tattatgttg gtccattggc gcgtggcaat agtcgttttg catggatgac
     4381 tcggaagtct gaagaaacaa ttaccccatg gaattttgaa gaagttgtcg ataaaggtgc
     4441 ttcagctcaa tcatttattg aacgcatgac aaactttgat aaaaatcttc caaatgaaaa
     4501 agtactacca aaacatagtt tgctttatga gtattttacg gtttataacg aattgacaaa
     4561 ggtcaaatat gttactgaag gaatgcgaaa accagcattt ctttcaggtg aacagaagaa
     4621 agccattgtt gatttactct tcaaaacaaa tcgaaaagta accgttaagc aattaaaaga
     4681 agattatttc aaaaaaatag aatgttttga tagtgttgaa atttcaggag ttgaagatag
     4741 atttaatgct tcattaggta cctaccatga tttgctaaaa attattaaag ataaagattt
     4801 tttggataat gaagaaaatg aagatatctt agaggatatt gttttaacat tgaccttatt
     4861 tgaagatagg gagatgattg aggaaagact taaaacatat gctcacctct ttgatgataa
     4921 ggtgatgaaa cagcttaaac gtcgccgtta tgctgctgct ggagctttgt ctcgaaaatt
     4981 gattaatggt attagggata agcaatctgg caaaacaata ttagattttt tgaaatcaga
     5041 tggttttgcc aatcgcaatt ttatgcagct gatccatgat gatagtttga catttaaaga
     5101 agacattcaa aaagcacaag tgtctggaca aggcgatagt ttacatgaac atattgcaaa
     5161 tttagctggt agccctgcta ttaaaaaagg tattttacag actgtaaaag ttgttgatga
     5221 attggtcaaa gtaatggggc ggcataagcc agaaaatatc gttattgaaa tggcacgtga
     5281 aaatcagaca actcaaaagg gccagaaaaa ttcgcgagag cgtatgaaac gaatcgaaga
     5341 aggtatcaaa gaattaggaa gtcagattct taaagagcat cctgttgaaa atactcaatt
     5401 gcaaaatgaa aagctctatc tctattatct ccaaaatgga agagacatgt atgtggacca
     5461 agaattagat attaatcgtt taagtgatta tgatgtcgat cacattgttc cacaaagttt
     5521 ccttaaagac gattcaatag acaataaggt cttaacgcgt tctgataaaa atcgtggtaa
     5581 atcggataac gttccaagtg aagaagtagt caaaaagatg aaaaactatt ggagacaact
     5641 tctaaacgcc aagttaatca ctcaacgtaa gtttgataat ttaacgaaag ctgaacgtgg
     5701 aggtttgagt gaacttgata aagctggttt tatcaaacgc caattggttg aaactcgcgc
     5761 tatcactaag catgtggcac aaattttgga tagtcgcatg aatactaaat acgatgaaaa
     5821 tgataaactt attcgagagg ttaaagtgat taccttaaaa tctaaattag tttctgactt
     5881 ccgaaaagat ttccaattct ataaagtacg tgagattaac aattaccatc atgcccatga
     5941 tgcgtatcta aatgccgtcg ttggaactgc tttgattaag aaatatccaa aacttgaatc
     6001 ggagtttgtc tatggtgatt ataaagttta tgatgttcgt aaaatgattg ctaagtctga
     6061 gcaagaaata ggcaaagcaa ccgcaaaata tttcttttac tctaatatca tgaacttctt
     6121 caaaacagaa attacacttg caaatggaga gattcgcaaa cgccctctaa tcgaaactaa
     6181 tggggaaact ggagaaattg tctgggataa agggcgagat tttgccacag tgcgcaaagt
     6241 attgtccatg ccccaagtca atattgtcaa gaaaacagaa gtacagacag gcggattctc
     6301 caaggagtca attttaccaa aaagaaattc ggacaagctt attgctcgta aaaaagactg
     6361 ggatccaaaa aaatatggtg gttttgatag tccaacggta gcttattcag tcctagtggt
     6421 tgctaaggtg gaaaaaggga aatcgaagaa gttaaaatcc gttaaagagt tactagggat
     6481 cacaattatg gaaagaagtt cctttgaaaa aaatccgatt gactttttag aagctaaagg
     6541 atataaggaa gttaaaaaag acttaatcat taaactacct aaatatagtc tttttgagtt
     6601 agaaaacggt cgtaaacgga tgctggctag tgccggagaa ttacaaaaag gaaatgagct
     6661 ggctctgcca agcaaatatg tgaatttttt atatttagct agtcattatg aaaagttgaa
     6721 gggtagtcca gaagataacg aacaaaaaca attgtttgtg gagcagcata agcattattt
     6781 agatgagatt attgagcaaa tcagtgaatt ttctaagcgt gttattttag cagatgccaa
     6841 tttagataaa gttcttagtg catataacaa acatagagac aaaccaatac gtgaacaagc
     6901 agaaaatatt attcatttat ttacgttgac gaatcttgga gctcccgctg cttttaaata
     6961 ttttgataca acaattgatc gtaaacgata tacgtctaca aaagaagttt tagatgccac
     7021 tcttatccat caatccatca ctggtcttta tgaaacacgc attgatttga gtcagctagg
     7081 aggtgactaa taacattgga agtggataac ggatccgcga tcgcggcgcg ccacctggtg
     7141 gccggccggt accacgcgtg cgcgctgatc cggctgctaa caaagcccga aaggaagctg
     7201 agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg
     7261 tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg
     7321 gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg
     7381 cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca
     7441 tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc
     7501 aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg
     7561 ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa
     7621 tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
     7681 ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     7741 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     7801 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     7861 gcttcaataa cattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     7921 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     7981 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     8041 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     8101 agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg
     8161 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     8221 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     8281 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     8341 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     8401 accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact
     8461 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     8521 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     8581 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     8641 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     8701 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     8761 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     8821 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     8881 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     8941 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     9001 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     9061 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     9121 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     9181 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     9241 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     9301 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     9361 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     9421 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     9481 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     9541 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     9601 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     9661 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca
     9721 tctgtgcggt atttcacacc gcaatggtgc actctcagta caatctgctc tgatgccgca
     9781 tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac
     9841 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
     9901 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
     9961 aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt
    10021 ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtc
//
