LOCUS       sequence-198021-        1270 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  express MBP hnRNPA2 LC with 4 R to K mutations.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1270)
  AUTHORS   Ryan VH, Dignon GL, Zerze GH, Chabata CV, Silva R, Conicella AE, 
            Amaya J, Burke KA, Mittal J, Fawzi NL
  TITLE     Mechanistic View of hnRNPA2 Low-Complexity Domain Structure, 
            Interactions, and Phase Separation Altered by Mutation and Arginine 
            Methylation.
  JOURNAL   Mol Cell. 2018 Jan 17. pii: S1097-2765(17)30979-6. doi: 
            10.1016/j.molcel.2017.12.022.
  PUBMED    29358076
REFERENCE   2  (bases 1 to 1270)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2018 Jan 17. pii: S1097-2765(17)30979-6. doi: 
            10.1016/j.molcel.2017.12.022."
COMMENT     Sequence Label: MBP-hnRNPA2_LC_R191K_R201K_R216K_R254K
FEATURES             Location/Qualifiers
     source          1..1270
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(33..50)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(522..542)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     protein_bind    complement(551..575)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(597..620)
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     regulatory      728..737
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     gap             1051..1066
                     /estimated_length=16
     gap             1122..1132
                     /estimated_length=11
     gap             1149..1158
                     /estimated_length=10
     gap             1226..1247
                     /estimated_length=22
     gap             1250..1261
                     /estimated_length=12
ORIGIN
        1 ttcttncggn ctttgttagc agccggatct cagtggtggt ggtggtggtg ctcgagttag
       61 taacggctgc ggccaccata gccgccagaa ccaccgctac cacccgggcc gtagttaccg
      121 ccaccatacg gaccacccat attacgggaa ccgccaaaat tgccggattt catcgggccg
      181 taattgctcg gttgctggtt gtagttgcca aaatcattgt aattgccgct accatagtta
      241 ccaccgccat agttatcgta gccaccacca taaccaccac cctggttacc gtagcctgga
      301 ccgccaccgc cataaccgcc tttaccaccg ccgtaacccg ggctaccacc gaaattgccg
      361 ccgcccggac caccaccgta accattatag ccatcgccaa agccgcgacc gctaccgtaa
      421 ccgtcggaac cacccttgaa attgctgccc ggacccggac cgaagttgcc gccaccaccc
      481 ttgctatcac cgaaaccaaa gttgccacct ttacccatat gaccctggaa gtacaggttc
      541 tcggtaccgc cttcagcttt tttgtacaaa cttgtgatat cagatcccga attagtctgc
      601 gcgtctttca gggcttcatc gacagtctga cgaccgctgg cggcgttgat caccgcagta
      661 cgcacggcat accagaaagc ggacatctgc gggatgttcg gcatgatttc acctttctgg
      721 gcgttttcca tggtggcggc aatacgtgga tctttcgcca actcttcctc gtaagacttc
      781 agcgctacgg cacccagcgg tttgtcttta ttaaccgctt ccagaccttc atcagtcagc
      841 agatagtttt cgaggaactc ttttgccagc tctttgttcg gactggcggc gttaatacct
      901 gcgctcagca cgccaacgaa cggtttggat ggttgaccct tgaaggtcgg cagtaccgtt
      961 acaccataat tcactttgct gggntcgatg ttggaccatg ccnacgggcc gttnatgnnn
     1021 atccntnttn cccnttnntn aangnnnnnt nnnnnnnnnn nnnnnncngg gncnnnntnc
     1081 nnnnnnnggt ttnnnnnngn nnnnangaan gnnaancccn tnnnnnnnnn nnttnnnncc
     1141 cnnnntttnn nnnnnnnntt nnntnnnnnt nnnngnnnan ccccnnannn aannanggnn
     1201 nnnnannnnn ttnnngnnna aannannnnn nnnnnnnnnn nnnnnnnccn nnnnnnnnnn
     1261 ntttnnntgg
//
