LOCUS       sequence-198022-         949 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  express His tagged P298L S329C hnRNPA2 LC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 949)
  AUTHORS   Ryan VH, Dignon GL, Zerze GH, Chabata CV, Silva R, Conicella AE, 
            Amaya J, Burke KA, Mittal J, Fawzi NL
  TITLE     Mechanistic View of hnRNPA2 Low-Complexity Domain Structure, 
            Interactions, and Phase Separation Altered by Mutation and Arginine 
            Methylation.
  JOURNAL   Mol Cell. 2018 Jan 17. pii: S1097-2765(17)30979-6. doi: 
            10.1016/j.molcel.2017.12.022.
  PUBMED    29358076
REFERENCE   2  (bases 1 to 949)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2018 Jan 17. pii: S1097-2765(17)30979-6. doi: 
            10.1016/j.molcel.2017.12.022."
COMMENT     Sequence Label: hnRNPA2_LC_P298L_S329C
FEATURES             Location/Qualifiers
     source          1..949
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             11..33
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             58..75
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             76..96
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             568..585
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(638..656)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      652..699
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ctannntnnt tttgtttaac tttaagaagg agatatacca tgggctctga taaaattcat
       61 catcatcatc atcacgaaaa tctttatttt caaggtcata tgggtcgtgg tggcaacttt
      121 ggtttcggtg atagccgcgg tggtggcggc aacttcggtc cgggtccggg cagcaatttc
      181 cgtggtggtt ccgacggtta cggtagcggt cgcggctttg gcgatggcta taatggttac
      241 ggtggtggtc cgggcggcgg caatttcggt ggtagcccgg gttacggcgg tggtcgcggc
      301 ggttatggcg gtggcggtcc aggctacggt aaccagggtg gtggttatgg tggtggctac
      361 gataactatg gcggtggtaa ctatggtagc ggcaattaca atgattttgg caactacaac
      421 cagcaactga gcaattacgg cccgatgaaa tccggcaatt ttggcggttc ccgtaatatg
      481 ggtggtccgt atggtggcgg taactacggc ccgggtggtt gcggtggttc tggcggctat
      541 ggtggccgca gccgttacta actcgagcac caccaccacc accactgaga tccggctgct
      601 aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa
      661 ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc
      721 ggattggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggnta
      781 cncgcancnn gaccgctaca cttgccagcg ncntagcgcc cgctcctttc nctttcttcc
      841 cttcctttct cnnacgntcg nnggnttncc ccnnnnagct ctaaatcagg ggnntccntt
      901 tanngttcnn nnntnnnnnn tttncngnnn nnnnnncncn aaannnnct
//
