LOCUS       sequence-198028-        1103 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of TDP-43 NTD Y4R.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Wang A, Conicella AE, Schmidt HB, Martin EW, Rhoads SN, Reeb AN, 
            Nourse A, Ramirez Montero D, Ryan VH, Rohatgi R, Shewmaker F, Naik 
            MT, Mittag T, Ayala YM, Fawzi NL
  TITLE     A single N-terminal phosphomimic disrupts TDP-43 polymerization, 
            phase separation, and RNA splicing.
  JOURNAL   EMBO J. 2018 Mar 1;37(5). pii: embj.201797452. doi: 
            10.15252/embj.201797452. Epub 2018 Feb 9.
  PUBMED    29438978
REFERENCE   2  (bases 1 to 1103)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2018-03-1"; volume: "37(5). pii: embj.201797452. doi"; pages:
            " 10.15252/embj.201797452. Epub 2018 Feb 9"
COMMENT     Sequence Label: TDP-43_NTD1-80_Y4R
FEATURES             Location/Qualifiers
     source          1..1103
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             63..80
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             81..101
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 tnnnnnctnn nnnaattttg tttaactttt gaaggagata taccatgggt agcgacaaga
       61 ttcatcacca ccatcatcat gagaacttat actttcaagg ccatatgatg agcgagagga
      121 ttcgtgttac cgaagatgag aacgacgagc cgatcgagat tccgagcgaa gatgacggta
      181 cggtcttgct gagcacggtg accgcgcagt tcccaggcgc ctgtggcctg cgctatcgta
      241 atccggtcag ccagtgcatg cgcggtgtcc gtctggttga gggcattctg cacgctccgg
      301 acgcgggctg gggcaatctg gtgtacgtgg tcaattaccc gaaagactag aagcgcaaga
      361 tggatgaaac tgacgccagc tctgcggtta aagtcaagcg tgcagtgtag aaaacgagcg
      421 acctggtcaa gcgtgcagtg tagaaaacga gcgacctgat cgtgctgggt ttgccgtgga
      481 aaaccacgga gcaagacctg aaagaatact ttagcacctt cggtgaggtg ctgatggtcc
      541 aggtaaagaa agatctgaaa accggtcaca gcaagggttt tggcttcgtc cgtttcaccg
      601 agtatgaaac ccaggtgaaa gttatgtcgc aacgtcacat gatcgacggt cgttggtgtg
      661 actgcaagct gccgaacagc aagcagagcc aagatgaacc gctgcgctcc cgtaaagttt
      721 tcgttggtcg ctgcaccgaa gatatgacgg aagatgagct gcgtgagttc ttttctcaat
      781 atggtgacgt gatggatgtt ttcatcccga aacctttccn gngcctttgc atttgttacc
      841 ttcgctgacg atcaaatcgc gcagagcctg tgtggtgang gatctgatta tnnaagnnat
      901 ttccgttcnc ntttctaacc gcgganccaa agcataacag caacccgccc aactgnnncg
      961 tttcnggtnn tttcggtggn aatccnnnnn nnntnngcaa cacggnngnn nttngcaatt
     1021 cnngnggcgg ngagngcggn ttgnncnata accanngnnc cannannnct gnnggtntnn
     1081 nnntnnggtg ccctccccct cca
//
