LOCUS       sequence-198030-         809 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of TDP-43 NTD E17R.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Wang A, Conicella AE, Schmidt HB, Martin EW, Rhoads SN, Reeb AN, 
            Nourse A, Ramirez Montero D, Ryan VH, Rohatgi R, Shewmaker F, Naik 
            MT, Mittag T, Ayala YM, Fawzi NL
  TITLE     A single N-terminal phosphomimic disrupts TDP-43 polymerization, 
            phase separation, and RNA splicing.
  JOURNAL   EMBO J. 2018 Mar 1;37(5). pii: embj.201797452. doi: 
            10.15252/embj.201797452. Epub 2018 Feb 9.
  PUBMED    29438978
REFERENCE   2  (bases 1 to 809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2018-03-1"; volume: "37(5). pii: embj.201797452. doi"; pages:
            " 10.15252/embj.201797452. Epub 2018 Feb 9"
COMMENT     Sequence Label: TDP-43_NTD1-80_E17R
FEATURES             Location/Qualifiers
     source          1..809
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             66..86
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ttttgtttaa cttttgaagg agatatacca tgggtagcga caagattcat caccaccatc
       61 atcatgagaa cttatacttt caaggccata tgatgagcga gtatattcgt gttaccgaag
      121 atgagaacga cgagccgatc aggattccga gcgaagatga cggtacggtc ttgctgagca
      181 cggtgaccgc gcagttccca ggcgcctgtg gcctgcgcta tcgtaatccg gtcagccagt
      241 gcatgcgcgg tgtccgtctg gttgagggca ttctgcacgc tccggacgcg ggctggggca
      301 atctggtgta cgtggtcaat tacccgaaag actagaagcg caagatggat gaaactgacg
      361 ccagctctgc ggttaaagtc aagcgtgcag tgtagaaaac gagcgacctg gtcaagcgtg
      421 cagtgtagaa aacgagcgac ctgatcgtgc tgggtttgcc gtggaaaacc acggagcaag
      481 acctgaaaga atactttagc accttcggtg aggtgctgat ggtccaggta aagaaagatc
      541 tgaaaaccgg tcacagcaag ggttttggct tcgtccgttt caccgagtat gaaacccagg
      601 tgaaagttat gtcgcaacgt cacatgatcg acggtcgttg gtgtgactgc aagctgccga
      661 acagcaagca gagccaagat gaaccgctgc gctcccgtaa agttttcgtt ggtcgctgca
      721 ccgaagatat gacggaagat gagctgcgtg agttcttttc tcaatatggt gacgtgatgg
      781 atgttttcat cccgaaacct ttccgcgcc
//
