LOCUS       sequence-198037-       10073 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression plasmid for SpCas9 Cluster 4 + Q926A variant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10073)
  AUTHORS   Chen JS, Dagdas YS, Kleinstiver BP, Welch MM, Sousa AA, Harrington 
            LB, Sternberg SH, Joung JK, Yildiz A, Doudna JA
  TITLE     Enhanced proofreading governs CRISPR-Cas9 targeting accuracy.
  JOURNAL   Nature. 2017 Sep 20. doi: 10.1038/nature24268.
  PUBMED    28931002
REFERENCE   2  (bases 1 to 10073)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2017 Sep 20. doi: 10.1038/nature24268."
COMMENT     Sequence Label: pJSC236 - Bacterial expression plasmid for SpC...
FEATURES             Location/Qualifiers
     source          1..10073
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     314..333
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     417..436
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        417..435
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             524..546
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             566..595
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             605..1705
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     primer_bind     1679..1702
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             1760..1780
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             1787..5893
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSAIERAAAFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRAITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     primer_bind     complement(6019..6037)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      6033..6080
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(6446..6451,6452..6468,6469..6474))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(6501..6519)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6587..6691
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6692..6760,6761..7552)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6910..6929)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7723..8311
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8212..8231
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8465..8482
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8497..8636
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8562..8581)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8722..8744
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(8738..8929)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca
       61 tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac
      121 gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga
      181 gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca
      241 cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc
      301 gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg
      361 tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat
      421 acgactcact atagggagac cacaacggtt tccctctagt gccggctccg gagagctctt
      481 taattaagcg gccgccctgc aggactcgag ttctagaaat aattttgttt aactttaaga
      541 aggagatata catatgaaat cttctcacca tcaccatcac catcaccatc accatggttc
      601 ttctatgaaa atcgaagaag gtaaactggt aatctggatt aacggcgata aaggctataa
      661 cggtctcgct gaagtcggta agaaattcga gaaagatacc ggaattaaag tcaccgttga
      721 gcatccggat aaactggaag agaaattccc acaggttgcg gcaactggcg atggccctga
      781 cattatcttc tgggcacacg accgctttgg tggctacgct caatctggcc tgttggctga
      841 aatcaccccg gacaaagcgt tccaggacaa gctgtatccg tttacctggg atgccgtacg
      901 ttacaacggc aagctgattg cttacccgat cgctgttgaa gcgttatcgc tgatttataa
      961 caaagatctg ctgccgaacc cgccaaaaac ctgggaagag atcccggcgc tggataaaga
     1021 actgaaagcg aaaggtaaga gcgcgctgat gttcaacctg caagaaccgt acttcacctg
     1081 gccgctgatt gctgctgacg ggggttatgc gttcaagtat gaaaacggca agtacgacat
     1141 taaagacgtg ggcgtggata acgctggcgc gaaagcgggt ctgaccttcc tggttgacct
     1201 gattaaaaac aaacacatga atgcagacac cgattactcc atcgcagaag ctgcctttaa
     1261 taaaggcgaa acagcgatga ccatcaacgg cccgtgggca tggtccaaca tcgacaccag
     1321 caaagtgaat tatggtgtaa cggtactgcc gaccttcaag ggtcaaccat ccaaaccgtt
     1381 cgttggcgtg ctgagcgcag gtattaacgc cgccagtccg aacaaagagc tggcaaaaga
     1441 gttcctcgaa aactatctgc tgactgatga aggtctggaa gcggttaata aagacaaacc
     1501 gctgggtgcc gtagcgctga agtcttacga ggaagagttg gcgaaagatc cacgtattgc
     1561 cgccactatg gaaaacgccc agaaaggtga aatcatgccg aacatcccgc agatgtccgc
     1621 tttctggtat gccgtgcgta ctgcggtgat caacgccgcc agcggtcgtc agactgtcga
     1681 tgaagccctg aaagacgcgc agactaattc gagctcgaac aacaacaaca ataacaataa
     1741 caacaacctc gggatcgagg aaaacctgta cttccaatcc aatgcaatgg ataagaaata
     1801 ctcaataggc ttagatatcg gcacaaatag cgtcggatgg gcggtgatca ctgatgaata
     1861 taaggttccg tctaaaaagt tcaaggttct gggaaataca gaccgccaca gtatcaaaaa
     1921 aaatcttata ggggctcttt tatttgacag tggagagaca gcggaagcga ctcgtctcaa
     1981 acggacagct cgtagaaggt atacacgtcg gaagaatcgt atttgttatc tacaggagat
     2041 tttttcaaat gagatggcga aagtagatga tagtttcttt catcgacttg aagagtcttt
     2101 tttggtggaa gaagacaaga agcatgaacg tcatcctatt tttggaaata tagtagatga
     2161 agttgcttat catgagaaat atccaactat ctatcatctg cgaaaaaaat tggtagattc
     2221 tactgataaa gcggatttgc gcttaatcta tttggcctta gcgcatatga ttaagtttcg
     2281 tggtcatttt ttgattgagg gagatttaaa tcctgataat agtgatgtgg acaaactatt
     2341 tatccagttg gtacaaacct acaatcaatt atttgaagaa aaccctatta acgcaagtgg
     2401 agtagatgct aaagcgattc tttctgcacg attgagtaaa tcaagacgat tagaaaatct
     2461 cattgctcag ctccccggtg agaagaaaaa tggcttattt gggaatctca ttgctttgtc
     2521 attgggtttg acccctaatt ttaaatcaaa ttttgatttg gcagaagatg ctaaattaca
     2581 gctttcaaaa gatacttacg atgatgattt agataattta ttggcgcaaa ttggagatca
     2641 atatgctgat ttgtttttgg cagctaagaa tttatcagat gctattttac tttcagatat
     2701 cctaagagta aatactgaaa taactaaggc tcccctatca gcttcaatga ttaaacgcta
     2761 cgatgaacat catcaagact tgactctttt aaaagcttta gttcgacaac aacttccaga
     2821 aaagtataaa gaaatctttt ttgatcaatc aaaaaacgga tatgcaggtt atattgatgg
     2881 gggagctagc caagaagaat tttataaatt tatcaaacca attttagaaa aaatggatgg
     2941 tactgaggaa ttattggtga aactaaatcg tgaagatttg ctgcgcaagc aacggacctt
     3001 tgacaacggc tctattcccc atcaaattca cttgggtgag ctgcatgcta ttttgagaag
     3061 acaagaagac ttttatccat ttttaaaaga caatcgtgag aagattgaaa aaatcttgac
     3121 ttttcgaatt ccttattatg ttggtccatt ggcgcgtggc aatagtcgtt ttgcatggat
     3181 gactcggaag tctgaagaaa caattacccc atggaatttt gaagaagttg tcgataaagg
     3241 tgcttcagct caatcagcta ttgaacgcgc tgctgctttt gataaaaatc ttccaaatga
     3301 aaaagtacta ccaaaacata gtttgcttta tgagtatttt acggtttata acgaattgac
     3361 aaaggtcaaa tatgttactg aaggaatgcg aaaaccagca tttctttcag gtgaacagaa
     3421 gaaagccatt gttgatttac tcttcaaaac aaatcgaaaa gtaaccgtta agcaattaaa
     3481 agaagattat ttcaaaaaaa tagaatgttt tgatagtgtt gaaatttcag gagttgaaga
     3541 tagatttaat gcttcattag gtacctacca tgatttgcta aaaattatta aagataaaga
     3601 ttttttggat aatgaagaaa atgaagatat cttagaggat attgttttaa cattgacctt
     3661 atttgaagat agggagatga ttgaggaaag acttaaaaca tatgctcacc tctttgatga
     3721 taaggtgatg aaacagctta aacgtcgccg ttatactggt tggggacgtt tgtctcgaaa
     3781 attgattaat ggtattaggg ataagcaatc tggcaaaaca atattagatt ttttgaaatc
     3841 agatggtttt gccaatcgca attttatgca gctgatccat gatgatagtt tgacatttaa
     3901 agaagacatt caaaaagcac aagtgtctgg acaaggcgat agtttacatg aacatattgc
     3961 aaatttagct ggtagccctg ctattaaaaa aggtatttta cagactgtaa aagttgttga
     4021 tgaattggtc aaagtaatgg ggcggcataa gccagaaaat atcgttattg aaatggcacg
     4081 tgaaaatcag acaactcaaa agggccagaa aaattcgcga gagcgtatga aacgaatcga
     4141 agaaggtatc aaagaattag gaagtcagat tcttaaagag catcctgttg aaaatactca
     4201 attgcaaaat gaaaagctct atctctatta tctccaaaat ggaagagaca tgtatgtgga
     4261 ccaagaatta gatattaatc gtttaagtga ttatgatgtc gatcacattg ttccacaaag
     4321 tttccttaaa gacgattcaa tagacaataa ggtcttaacg cgttctgata aaaatcgtgg
     4381 taaatcggat aacgttccaa gtgaagaagt agtcaaaaag atgaaaaact attggagaca
     4441 acttctaaac gccaagttaa tcactcaacg taagtttgat aatttaacga aagctgaacg
     4501 tggaggtttg agtgaacttg ataaagctgg ttttatcaaa cgccaattgg ttgaaactcg
     4561 cgctatcact aagcatgtgg cacaaatttt ggatagtcgc atgaatacta aatacgatga
     4621 aaatgataaa cttattcgag aggttaaagt gattacctta aaatctaaat tagtttctga
     4681 cttccgaaaa gatttccaat tctataaagt acgtgagatt aacaattacc atcatgccca
     4741 tgatgcgtat ctaaatgccg tcgttggaac tgctttgatt aagaaatatc caaaacttga
     4801 atcggagttt gtctatggtg attataaagt ttatgatgtt cgtaaaatga ttgctaagtc
     4861 tgagcaagaa ataggcaaag caaccgcaaa atatttcttt tactctaata tcatgaactt
     4921 cttcaaaaca gaaattacac ttgcaaatgg agagattcgc aaacgccctc taatcgaaac
     4981 taatggggaa actggagaaa ttgtctggga taaagggcga gattttgcca cagtgcgcaa
     5041 agtattgtcc atgccccaag tcaatattgt caagaaaaca gaagtacaga caggcggatt
     5101 ctccaaggag tcaattttac caaaaagaaa ttcggacaag cttattgctc gtaaaaaaga
     5161 ctgggatcca aaaaaatatg gtggttttga tagtccaacg gtagcttatt cagtcctagt
     5221 ggttgctaag gtggaaaaag ggaaatcgaa gaagttaaaa tccgttaaag agttactagg
     5281 gatcacaatt atggaaagaa gttcctttga aaaaaatccg attgactttt tagaagctaa
     5341 aggatataag gaagttaaaa aagacttaat cattaaacta cctaaatata gtctttttga
     5401 gttagaaaac ggtcgtaaac ggatgctggc tagtgccgga gaattacaaa aaggaaatga
     5461 gctggctctg ccaagcaaat atgtgaattt tttatattta gctagtcatt atgaaaagtt
     5521 gaagggtagt ccagaagata acgaacaaaa acaattgttt gtggagcagc ataagcatta
     5581 tttagatgag attattgagc aaatcagtga attttctaag cgtgttattt tagcagatgc
     5641 caatttagat aaagttctta gtgcatataa caaacataga gacaaaccaa tacgtgaaca
     5701 agcagaaaat attattcatt tatttacgtt gacgaatctt ggagctcccg ctgcttttaa
     5761 atattttgat acaacaattg atcgtaaacg atatacgtct acaaaagaag ttttagatgc
     5821 cactcttatc catcaatcca tcactggtct ttatgaaaca cgcattgatt tgagtcagct
     5881 aggaggtgac taataacatt ggaagtggat aacggatccg cgatcgcggc gcgccacctg
     5941 gtggccggcc ggtaccacgc gtgcgcgctg atccggctgc taacaaagcc cgaaaggaag
     6001 ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac
     6061 gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt
     6121 gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg
     6181 cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac
     6241 gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc
     6301 cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg
     6361 gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag
     6421 caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga
     6481 gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
     6541 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     6601 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     6661 aatgcttcaa taacattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     6721 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     6781 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     6841 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     6901 taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg
     6961 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     7021 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     7081 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     7141 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     7201 cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa
     7261 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     7321 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     7381 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     7441 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     7501 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     7561 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     7621 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     7681 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     7741 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     7801 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     7861 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     7921 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     7981 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     8041 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     8101 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     8161 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     8221 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     8281 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     8341 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     8401 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     8461 gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac
     8521 gcatctgtgc ggtatttcac accgcaatgg tgcactctca gtacaatctg ctctgatgcc
     8581 gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc
     8641 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     8701 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     8761 cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga
     8821 tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc
     8881 ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg
     8941 tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca
     9001 cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac
     9061 tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg
     9121 ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga
     9181 acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga
     9241 agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc
     9301 gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg
     9361 tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga
     9421 tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg
     9481 tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc
     9541 cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg
     9601 acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt
     9661 ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccaa tgatcgaagt
     9721 taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg
     9781 cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat
     9841 aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc
     9901 ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt
     9961 gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat
    10021 cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccg
//
