LOCUS       sequence-198053-       10073 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression plasmid for SpCas9-HF1, REC2 FRET variant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10073)
  AUTHORS   Chen JS, Dagdas YS, Kleinstiver BP, Welch MM, Sousa AA, Harrington 
            LB, Sternberg SH, Joung JK, Yildiz A, Doudna JA
  TITLE     Enhanced proofreading governs CRISPR-Cas9 targeting accuracy.
  JOURNAL   Nature. 2017 Sep 20. doi: 10.1038/nature24268.
  PUBMED    28931002
REFERENCE   2  (bases 1 to 10073)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2017 Sep 20. doi: 10.1038/nature24268."
COMMENT     Sequence Label: pJSC129 - Bacterial expression plasmid for
            SpC...
FEATURES             Location/Qualifiers
     source          1..10073
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     120..139
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     223..242
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        223..241
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             330..352
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             372..401
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             411..1511
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     primer_bind     1485..1508
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             1566..1586
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             1593..5699
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETACATRLKRTARRRYTRRKNRISYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDCDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTAFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIESFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGALSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     ALIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRAITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     primer_bind     complement(5825..5843)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      5839..5886
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(6252..6257,6258..6274,6275..6280))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(6307..6325)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6393..6497
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6498..6566,6567..7358)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6716..6735)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7529..8117
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8018..8037
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8271..8288
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8303..8442
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8368..8387)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8528..8550
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(8544..8735)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc
       61 atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg
      121 gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg
      181 atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa attaatacga ctcactatag
      241 ggagaccaca acggtttccc tctagtgccg gctccggaga gctctttaat taagcggccg
      301 ccctgcagga ctcgagttct agaaataatt ttgtttaact ttaagaagga gatatacata
      361 tgaaatcttc tcaccatcac catcaccatc accatcacca tggttcttct atgaaaatcg
      421 aagaaggtaa actggtaatc tggattaacg gcgataaagg ctataacggt ctcgctgaag
      481 tcggtaagaa attcgagaaa gataccggaa ttaaagtcac cgttgagcat ccggataaac
      541 tggaagagaa attcccacag gttgcggcaa ctggcgatgg ccctgacatt atcttctggg
      601 cacacgaccg ctttggtggc tacgctcaat ctggcctgtt ggctgaaatc accccggaca
      661 aagcgttcca ggacaagctg tatccgttta cctgggatgc cgtacgttac aacggcaagc
      721 tgattgctta cccgatcgct gttgaagcgt tatcgctgat ttataacaaa gatctgctgc
      781 cgaacccgcc aaaaacctgg gaagagatcc cggcgctgga taaagaactg aaagcgaaag
      841 gtaagagcgc gctgatgttc aacctgcaag aaccgtactt cacctggccg ctgattgctg
      901 ctgacggggg ttatgcgttc aagtatgaaa acggcaagta cgacattaaa gacgtgggcg
      961 tggataacgc tggcgcaaaa gcgggtctga ccttcctggt tgacctgatt aaaaacaaac
     1021 acatgaatgc agacaccgat tactccatcg cagaagctgc ctttaataaa ggcgaaacag
     1081 cgatgaccat caacggcccg tgggcatggt ccaacatcga caccagcaaa gtgaattatg
     1141 gtgtaacggt actgccgacc ttcaagggtc aaccatccaa accgttcgtt ggcgtgctga
     1201 gcgcaggtat taacgccgcc agtccgaaca aagagctggc aaaagagttc ctcgaaaact
     1261 atctgctgac tgatgaaggt ctggaagcgg ttaataaaga caaaccgctg ggtgccgtag
     1321 cgctgaagtc ttacgaggaa gagttggcga aagatccacg tattgccgcc actatggaaa
     1381 acgcccagaa aggtgaaatc atgccgaaca tcccgcagat gtccgctttc tggtatgccg
     1441 tgcgtactgc ggtgatcaac gccgccagcg gtcgtcagac tgtcgatgaa gccctgaaag
     1501 acgcgcagac taattcgagc tcgaacaaca acaacaataa caataacaac aacctcggga
     1561 tcgaggaaaa cctgtacttc caatccaatg caatggataa gaaatactca ataggcttag
     1621 atatcggcac aaatagcgtc ggatgggcgg tgatcactga tgaatataag gttccgtcta
     1681 aaaagttcaa ggttctggga aatacagacc gccacagtat caaaaaaaat cttatagggg
     1741 ctcttttatt tgacagtgga gagacagcgt gtgcgactcg tctcaaacgg acagctcgta
     1801 gaaggtatac acgtcggaag aatcgtattt cttatctaca ggagattttt tcaaatgaga
     1861 tggcgaaagt agatgatagt ttctttcatc gacttgaaga gtcttttttg gtggaagaag
     1921 acaagaagca tgaacgtcat cctatttttg gaaatatagt agatgaagtt gcttatcatg
     1981 agaaatatcc aactatctat catctgcgaa aaaaattggt agattctact gataaagcgg
     2041 atttgcgctt aatctatttg gccttagcgc atatgattaa gtttcgtggt cattttttga
     2101 ttgagggaga tttaaatcct gataatagtg atgtggacaa actatttatc cagttggtac
     2161 aaacctacaa tcaattattt gaagaaaacc ctattaacgc aagtggagta gatgctaaag
     2221 cgattctttc tgcacgattg agtaaatcaa gacgattaga aaatctcatt gctcagctcc
     2281 ccggtgagaa gaaaaatggc ttatttggga atctcattgc tttgtcattg ggtttgaccc
     2341 ctaattttaa atcaaatttt gatttggcag aagatgctaa attacagctt tcaaaagata
     2401 cttacgattg tgatttagat aatttattgg cgcaaattgg agatcaatat gctgatttgt
     2461 ttttggcagc taagaattta tcagatgcta ttttactttc agatatccta agagtaaata
     2521 ctgaaataac taaggctccc ctatcagctt caatgattaa acgctacgat gaacatcatc
     2581 aagacttgac tcttttaaaa gctttagttc gacaacaact tccagaaaag tataaagaaa
     2641 tcttttttga tcaatcaaaa aacggatatg caggttatat tgatggggga gctagccaag
     2701 aagaatttta taaatttatc aaaccaattt tagaaaaaat ggatggtact gaggaattat
     2761 tggtgaaact aaatcgtgaa gatttgctgc gcaagcaacg gacctttgac aacggctcta
     2821 ttccccatca aattcacttg ggtgagctgc atgctatttt gagaagacaa gaagactttt
     2881 atccattttt aaaagacaat cgtgagaaga ttgaaaaaat cttgactttt cgaattcctt
     2941 attatgttgg tccattggcg cgtggcaata gtcgttttgc atggatgact cggaagtctg
     3001 aagaaacaat taccccatgg aattttgaag aagttgtcga taaaggtgct tcagctcaat
     3061 catttattga acgcatgaca gcgtttgata aaaatcttcc aaatgaaaaa gtactaccaa
     3121 aacatagttt gctttatgag tattttacgg tttataacga attgacaaag gtcaaatatg
     3181 ttactgaagg aatgcgaaaa ccagcatttc tttcaggtga acagaagaaa gccattgttg
     3241 atttactctt caaaacaaat cgaaaagtaa ccgttaagca attaaaagaa gattatttca
     3301 aaaaaataga atcttttgat agtgttgaaa tttcaggagt tgaagataga tttaatgctt
     3361 cattaggtac ctaccatgat ttgctaaaaa ttattaaaga taaagatttt ttggataatg
     3421 aagaaaatga agatatctta gaggatattg ttttaacatt gaccttattt gaagataggg
     3481 agatgattga ggaaagactt aaaacatatg ctcacctctt tgatgataag gtgatgaaac
     3541 agcttaaacg tcgccgttat actggttggg gagcgttgtc tcgaaaattg attaatggta
     3601 ttagggataa gcaatctggc aaaacaatat tagatttttt gaaatcagat ggttttgcca
     3661 atcgcaattt tatggccctg atccatgatg atagtttgac atttaaagaa gacattcaaa
     3721 aagcacaagt gtctggacaa ggcgatagtt tacatgaaca tattgcaaat ttagctggta
     3781 gccctgctat taaaaaaggt attttacaga ctgtaaaagt tgttgatgaa ttggtcaaag
     3841 taatggggcg gcataagcca gaaaatatcg ttattgaaat ggcacgtgaa aatcagacaa
     3901 ctcaaaaggg ccagaaaaat tcgcgagagc gtatgaaacg aatcgaagaa ggtatcaaag
     3961 aattaggaag tcagattctt aaagagcatc ctgttgaaaa tactcaattg caaaatgaaa
     4021 agctctatct ctattatctc caaaatggaa gagacatgta tgtggaccaa gaattagata
     4081 ttaatcgttt aagtgattat gatgtcgatc acattgttcc acaaagtttc cttaaagacg
     4141 attcaataga caataaggtc ttaacgcgtt ctgataaaaa tcgtggtaaa tcggataacg
     4201 ttccaagtga agaagtagtc aaaaagatga aaaactattg gagacaactt ctaaacgcca
     4261 agttaatcac tcaacgtaag tttgataatt taacgaaagc tgaacgtgga ggtttgagtg
     4321 aacttgataa agctggtttt atcaaacgcc aattggttga aactcgcgcc atcactaagc
     4381 atgtggcaca aattttggat agtcgcatga atactaaata cgatgaaaat gataaactta
     4441 ttcgagaggt taaagtgatt accttaaaat ctaaattagt ttctgacttc cgaaaagatt
     4501 tccaattcta taaagtacgt gagattaaca attaccatca tgcccatgat gcgtatctaa
     4561 atgccgtcgt tggaactgct ttgattaaga aatatccaaa acttgaatcg gagtttgtct
     4621 atggtgatta taaagtttat gatgttcgta aaatgattgc taagtctgag caagaaatag
     4681 gcaaagcaac cgcaaaatat ttcttttact ctaatatcat gaacttcttc aaaacagaaa
     4741 ttacacttgc aaatggagag attcgcaaac gccctctaat cgaaactaat ggggaaactg
     4801 gagaaattgt ctgggataaa gggcgagatt ttgccacagt gcgcaaagta ttgtccatgc
     4861 cccaagtcaa tattgtcaag aaaacagaag tacagacagg cggattctcc aaggagtcaa
     4921 ttttaccaaa aagaaattcg gacaagctta ttgctcgtaa aaaagactgg gatccaaaaa
     4981 aatatggtgg ttttgatagt ccaacggtag cttattcagt cctagtggtt gctaaggtgg
     5041 aaaaagggaa atcgaagaag ttaaaatccg ttaaagagtt actagggatc acaattatgg
     5101 aaagaagttc ctttgaaaaa aatccgattg actttttaga agctaaagga tataaggaag
     5161 ttaaaaaaga cttaatcatt aaactaccta aatatagtct ttttgagtta gaaaacggtc
     5221 gtaaacggat gctggctagt gccggagaat tacaaaaagg aaatgagctg gctctgccaa
     5281 gcaaatatgt gaatttttta tatttagcta gtcattatga aaagttgaag ggtagtccag
     5341 aagataacga acaaaaacaa ttgtttgtgg agcagcataa gcattattta gatgagatta
     5401 ttgagcaaat cagtgaattt tctaagcgtg ttattttagc agatgccaat ttagataaag
     5461 ttcttagtgc atataacaaa catagagaca aaccaatacg tgaacaagca gaaaatatta
     5521 ttcatttatt tacgttgacg aatcttggag ctcccgctgc ttttaaatat tttgatacaa
     5581 caattgatcg taaacgatat acgtctacaa aagaagtttt agatgccact cttatccatc
     5641 aatccatcac tggtctttat gaaacacgca ttgatttgag tcagctagga ggtgactaat
     5701 aacattggaa gtggataacg gatccgcgat cgcggcgcgc cacctggtgg ccggccggta
     5761 ccacgcgtgc gcgctgatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct
     5821 gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt
     5881 tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat
     5941 cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg
     6001 gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag
     6061 cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg
     6121 cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac
     6181 gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga
     6241 taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac
     6301 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     6361 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     6421 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataac
     6481 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     6541 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     6601 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     6661 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     6721 gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact
     6781 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     6841 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     6901 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     6961 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     7021 agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     7081 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     7141 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     7201 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     7261 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     7321 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     7381 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     7441 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     7501 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     7561 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     7621 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
     7681 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     7741 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     7801 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     7861 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     7921 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     7981 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     8041 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     8101 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     8161 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     8221 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     8281 tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
     8341 tttcacaccg caatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     8401 gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca
     8461 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
     8521 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg
     8581 cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca
     8641 tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg
     8701 gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt
     8761 ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact
     8821 gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg
     8881 cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta
     8941 ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag
     9001 ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt
     9061 gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt
     9121 gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg
     9181 agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg
     9241 ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca
     9301 gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc
     9361 cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga
     9421 ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg
     9481 aggcggcata aatcgccgtg acgatcagcg gtccaatgat cgaagttagg ctggtaagag
     9541 ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg
     9601 cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca
     9661 tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg
     9721 cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag
     9781 cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc
     9841 gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca
     9901 tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg
     9961 agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct
    10021 gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gca
//
