LOCUS       sequence-198153-        1809 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  LI Gene of Interest module.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Wu D, Schandry N, Lahaye T
  TITLE     A modular toolbox for Golden-Gate-based plasmid assembly streamlines
            generation of Ralstonia solanacearum species complex knockout 
            strains and multi-cassette complementation constructs.
  JOURNAL   Mol Plant Pathol. 2017 Oct 27. doi: 10.1111/mpp.12632.
  PUBMED    29077245
REFERENCE   2  (bases 1 to 1809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Plant 
            Pathol. 2017 Oct 27. doi: 10.1111/mpp.12632."
COMMENT     Sequence Label: LI C-D GUS
FEATURES             Location/Qualifiers
     source          1..1809
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..1809
                     /codon_start=1
                     /gene="uidA (or gusA) from E. coli"
                     /product="beta-glucuronidase"
                     /label=GUS
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
                     AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
                     FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQF
                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
                     ADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
                     KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD
                     LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY
                     TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK
                     PKSAAFLLQKRWTGMNFGEKPQQGGKQ"
ORIGIN
        1 atgttacgtc ctgtagaaac cccaacccgt gaaatcaaaa aactcgacgg cctgtgggca
       61 ttcagtctgg atcgcgaaaa ctgtggaatt gatcagcgtt ggtgggaaag cgcgttacaa
      121 gaaagccggg caattgctgt gccaggcagt tttaacgatc agttcgccga tgcagatatt
      181 cgtaattatg cgggcaacgt ctggtatcag cgcgaagtct ttataccgaa aggttgggca
      241 ggccagcgta tcgtgctgcg tttcgatgcg gtcactcatt acggcaaagt gtgggtcaat
      301 aatcaggaag tgatggagca tcagggcggc tatacgccat ttgaagccga tgtcacgccg
      361 tatgttattg ccgggaaaag tgtacgtatc accgtttgtg tgaacaacga actgaactgg
      421 cagactatcc cgccgggaat ggtgattacc gacgaaaacg gcaagaaaaa gcagtcttac
      481 ttccatgatt tctttaacta tgccggaatc catcgcagcg taatgctcta caccacgccg
      541 aacacctggg tggacgatat caccgtggtg acgcatgtcg cgcaagactg taaccacgcg
      601 tctgttgact ggcaggtggt ggccaatggt gatgtcagcg ttgaactgcg tgatgcggat
      661 caacaggtgg ttgcaactgg acaaggcact agcgggactt tgcaagtggt gaatccgcac
      721 ctctggcaac cgggtgaagg ttatctctat gaactgtgcg tcacagccaa aagccagaca
      781 gagtgtgata tctacccgct tcgcgtcggc atccggtcag tggcagtgaa gggcgaacag
      841 ttcctgatta accacaaacc gttctacttt actggctttg gtcgtcatga agatgcggac
      901 ttgcgtggca aaggattcga taacgtgctg atggtgcacg accacgcatt aatggactgg
      961 attggggcca actcctaccg tacctcgcat tacccttacg ctgaagagat gctcgactgg
     1021 gcagatgaac atggcatcgt ggtgattgat gaaactgctg ctgtcggctt taacctctct
     1081 ttaggcattg gtttcgaagc gggcaacaag ccgaaagaac tgtacagcga agaggcagtc
     1141 aacggggaaa ctcagcaagc gcacttacag gcgattaaag agctgatagc gcgtgacaaa
     1201 aaccacccaa gcgtggtgat gtggagtatt gccaacgaac cggatacccg tccgcaaggt
     1261 gcacgggaat atttcgcgcc actggcggaa gcaacgcgta aactcgaccc gacgcgtccg
     1321 atcacctgcg tcaatgtaat gttctgcgac gctcacaccg ataccatcag cgatctcttt
     1381 gatgtgctgt gcctgaaccg ttattacgga tggtatgtcc aaagcggcga tttggaaacg
     1441 gcagagaagg tactggaaaa agaacttctg gcctggcagg agaaactgca tcagccgatt
     1501 atcatcaccg aatacggcgt ggatacgtta gccgggctgc actcaatgta caccgacatg
     1561 tggagtgaag agtatcagtg tgcatggctg gatatgtatc accgcgtctt tgatcgcgtc
     1621 agcgccgtcg tcggtgaaca ggtatggaat ttcgccgatt ttgcgacctc gcaaggcata
     1681 ttgcgcgttg gcggtaacaa gaaagggatc ttcactcgcg accgcaaacc gaagtcggcg
     1741 gcttttctgc tgcaaaaacg ctggactggc atgaacttcg gtgaaaaacc gcagcaggga
     1801 ggcaaacaa
//
