LOCUS       sequence-198181-       16984 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The dCas9-TET1CD fusion protein is cloned into the pINDUCER 
            lentiviral backbone (Addgene plasmid# 46948)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16984)
  AUTHORS   Verma N, Pan H, Dore LC, Shukla A, Li QV, Pelham-Webb B, Teijeiro V,
            Gonzalez F, Krivtsov A, Chang CJ, Papapetrou EP, He C, Elemento O, 
            Huangfu D
  TITLE     TET proteins safeguard bivalent promoters from de novo methylation 
            in human embryonic stem cells.
  JOURNAL   Nat Genet. 2018 Jan;50(1):83-95. doi: 10.1038/s41588-017-0002-y. 
            Epub 2017 Dec 4.
  PUBMED    29203910
REFERENCE   2  (bases 1 to 16984)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41588-017-0002-y"; journalName: "Nat Genet"; date: 
            "2018-01"; volume: "50"; issue: "1"; pages: "83-95"
COMMENT     Sequence Label: pINDUCER dCas9-TET1CD
FEATURES             Location/Qualifiers
     source          1..16984
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2230..2248,2249..2271,2272..2290,2291..2313,2314..2332,
                     2333..2355,2356..2374,2375..2397,2398..2416,2417..2439,
                     2440..2458,2459..2481,2482..2500)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2533..2571
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2568..2592
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2701..2725
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      2750..2759
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2759..2824
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             2831..2851
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(2876..2899,2900..2902,2903..5389,5390..5392,
                     5393..6976)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             2876..6976
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     protein_bind    complement(9217..9241)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             9294..9320
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        9540..10718
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          9771..10709
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     10666..10686
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    10788..10821
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             join(10828..11445,11446..11451,11452..11487,11488..11490,
                     11491..11526,11527..11529,11530..11565,11566..11574)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    11631..12196
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(11790..11807)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     12017..12036
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(12212..12214,12215..12217,12218..12931)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(12212..12214,12215..12217,12218..12928)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(12518..12537)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     12865..12886
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     13067..13086
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    13374..13962
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(13427..13447)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(13845..13856)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             14037..14270
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(14389..14407)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        14475..14579
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(14580..14648,14649..15440)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(14798..14817)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      15611..16199
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     16100..16119
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     16353..16370
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    16610..16631
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(16646..16651,16652..16669,16670..16676)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    16684..16700
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     16689..16711
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     16708..16724
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     16708..16724
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     16774..16793
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactaggatc
     2221 ctttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca
     2281 gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa
     2341 agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc
     2401 ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa
     2461 agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagctcgg
     2521 tacccgggtc gaggtaggcg tgtacggtgg gaggcctata taagcagagc tcgtttagtg
     2581 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg
     2641 gaccgatcca gcctccgcgg ccccgaacta gtccagtgtg gtggaattct gcagatatca
     2701 acaagtttgt acaaaaaagc aggctccgcg gttttcaggt tggaccggtg ccaccatgga
     2761 ctataaggac cacgacggag actacaagga tcatgatatt gattacaaag acgatgacga
     2821 taagatggcc ccaaagaaga agcggaaggt cggtatccac ggagtcccag cagccgacaa
     2881 gaagtacagc atcggcctgg ccatcggcac caactctgtg ggctgggccg tgatcaccga
     2941 cgagtacaag gtgcccagca agaaattcaa ggtgctgggc aacaccgacc ggcacagcat
     3001 caagaagaac ctgatcggag ccctgctgtt cgacagcggc gaaacagccg aggccacccg
     3061 gctgaagaga accgccagaa gaagatacac cagacggaag aaccggatct gctatctgca
     3121 agagatcttc agcaacgaga tggccaaggt ggacgacagc ttcttccaca gactggaaga
     3181 gtccttcctg gtggaagagg ataagaagca cgagcggcac cccatcttcg gcaacatcgt
     3241 ggacgaggtg gcctaccacg agaagtaccc caccatctac cacctgagaa agaaactggt
     3301 ggacagcacc gacaaggccg acctgcggct gatctatctg gccctggccc acatgatcaa
     3361 gttccggggc cacttcctga tcgagggcga cctgaacccc gacaacagcg acgtggacaa
     3421 gctgttcatc cagctggtgc agacctacaa ccagctgttc gaggaaaacc ccatcaacgc
     3481 cagcggcgtg gacgccaagg ccatcctgtc tgccagactg agcaagagca gacggctgga
     3541 aaatctgatc gcccagctgc ccggcgagaa gaagaatggc ctgttcggaa acctgattgc
     3601 cctgagcctg ggcctgaccc ccaacttcaa gagcaacttc gacctggccg aggatgccaa
     3661 actgcagctg agcaaggaca cctacgacga cgacctggac aacctgctgg cccagatcgg
     3721 cgaccagtac gccgacctgt ttctggccgc caagaacctg tccgacgcca tcctgctgag
     3781 cgacatcctg agagtgaaca ccgagatcac caaggccccc ctgagcgcct ctatgatcaa
     3841 gagatacgac gagcaccacc aggacctgac cctgctgaaa gctctcgtgc ggcagcagct
     3901 gcctgagaag tacaaagaga ttttcttcga ccagagcaag aacggctacg ccggctacat
     3961 tgacggcgga gccagccagg aagagttcta caagttcatc aagcccatcc tggaaaagat
     4021 ggacggcacc gaggaactgc tcgtgaagct gaacagagag gacctgctgc ggaagcagcg
     4081 gaccttcgac aacggcagca tcccccacca gatccacctg ggagagctgc acgccattct
     4141 gcggcggcag gaagattttt acccattcct gaaggacaac cgggaaaaga tcgagaagat
     4201 cctgaccttc cgcatcccct actacgtggg ccctctggcc aggggaaaca gcagattcgc
     4261 ctggatgacc agaaagagcg aggaaaccat caccccctgg aacttcgagg aagtggtgga
     4321 caagggcgct tccgcccaga gcttcatcga gcggatgacc aacttcgata agaacctgcc
     4381 caacgagaag gtgctgccca agcacagcct gctgtacgag tacttcaccg tgtataacga
     4441 gctgaccaaa gtgaaatacg tgaccgaggg aatgagaaag cccgccttcc tgagcggcga
     4501 gcagaaaaag gccatcgtgg acctgctgtt caagaccaac cggaaagtga ccgtgaagca
     4561 gctgaaagag gactacttca agaaaatcga gtgcttcgac tccgtggaaa tctccggcgt
     4621 ggaagatcgg ttcaacgcct ccctgggcac ataccacgat ctgctgaaaa ttatcaagga
     4681 caaggacttc ctggacaatg aggaaaacga ggacattctg gaagatatcg tgctgaccct
     4741 gacactgttt gaggacagag agatgatcga ggaacggctg aaaacctatg cccacctgtt
     4801 cgacgacaaa gtgatgaagc agctgaagcg gcggagatac accggctggg gcaggctgag
     4861 ccggaagctg atcaacggca tccgggacaa gcagtccggc aagacaatcc tggatttcct
     4921 gaagtccgac ggcttcgcca acagaaactt catgcagctg atccacgacg acagcctgac
     4981 ctttaaagag gacatccaga aagcccaggt gtccggccag ggcgatagcc tgcacgagca
     5041 cattgccaat ctggccggca gccccgccat taagaagggc atcctgcaga cagtgaaggt
     5101 ggtggacgag ctcgtgaaag tgatgggccg gcacaagccc gagaacatcg tgatcgaaat
     5161 ggccagagag aaccagacca cccagaaggg acagaagaac agccgcgaga gaatgaagcg
     5221 gatcgaagag ggcatcaaag agctgggcag ccagatcctg aaagaacacc ccgtggaaaa
     5281 cacccagctg cagaacgaga agctgtacct gtactacctg cagaatgggc gggatatgta
     5341 cgtggaccag gaactggaca tcaaccggct gtccgactac gatgtggacg ctatcgtgcc
     5401 tcagagcttt ctgaaggacg actccatcga caacaaggtg ctgaccagaa gcgacaagaa
     5461 ccggggcaag agcgacaacg tgccctccga agaggtcgtg aagaagatga agaactactg
     5521 gcggcagctg ctgaacgcca agctgattac ccagagaaag ttcgacaatc tgaccaaggc
     5581 cgagagaggc ggcctgagcg aactggataa ggccggcttc atcaagagac agctggtgga
     5641 aacccggcag atcacaaagc acgtggcaca gatcctggac tcccggatga acactaagta
     5701 cgacgagaat gacaagctga tccgggaagt gaaagtgatc accctgaagt ccaagctggt
     5761 gtccgatttc cggaaggatt tccagtttta caaagtgcgc gagatcaaca actaccacca
     5821 cgcccacgac gcctacctga acgccgtcgt gggaaccgcc ctgatcaaaa agtaccctaa
     5881 gctggaaagc gagttcgtgt acggcgacta caaggtgtac gacgtgcgga agatgatcgc
     5941 caagagcgag caggaaatcg gcaaggctac cgccaagtac ttcttctaca gcaacatcat
     6001 gaactttttc aagaccgaga ttaccctggc caacggcgag atccggaagc ggcctctgat
     6061 cgagacaaac ggcgaaaccg gggagatcgt gtgggataag ggccgggatt ttgccaccgt
     6121 gcggaaagtg ctgagcatgc cccaagtgaa tatcgtgaaa aagaccgagg tgcagacagg
     6181 cggcttcagc aaagagtcta tcctgcccaa gaggaacagc gataagctga tcgccagaaa
     6241 gaaggactgg gaccctaaga agtacggcgg cttcgacagc cccaccgtgg cctattctgt
     6301 gctggtggtg gccaaagtgg aaaagggcaa gtccaagaaa ctgaagagtg tgaaagagct
     6361 gctggggatc accatcatgg aaagaagcag cttcgagaag aatcccatcg actttctgga
     6421 agccaagggc tacaaagaag tgaaaaagga cctgatcatc aagctgccta agtactccct
     6481 gttcgagctg gaaaacggcc ggaagagaat gctggcctct gccggcgaac tgcagaaggg
     6541 aaacgaactg gccctgccct ccaaatatgt gaacttcctg tacctggcca gccactatga
     6601 gaagctgaag ggctcccccg aggataatga gcagaaacag ctgtttgtgg aacagcacaa
     6661 gcactacctg gacgagatca tcgagcagat cagcgagttc tccaagagag tgatcctggc
     6721 cgacgctaat ctggacaaag tgctgtccgc ctacaacaag caccgggata agcccatcag
     6781 agagcaggcc gagaatatca tccacctgtt taccctgacc aatctgggag cccctgccgc
     6841 cttcaagtac tttgacacca ccatcgaccg gaagaggtac accagcacca aagaggtgct
     6901 ggacgccacc ctgatccacc agagcatcac cggcctgtac gagacacgga tcgacctgtc
     6961 tcagctggga ggcgacaaaa ggccggcggc cacgaaaaag gccggccagc tgcccacctg
     7021 cagctgtctt gatcgagtta tacaaaaaga caaaggccca tattatacac accttggggc
     7081 aggaccaagt gttgctgctg tcagggaaat catggagaat aggtatggtc aaaaaggaaa
     7141 cgcaataagg atagaaatag tagtgtacac cggtaaagaa gggaaaagct ctcatgggtg
     7201 tccaattgct aagtgggttt taagaagaag cagtgatgaa gaaaaagttc tttgtttggt
     7261 ccggcagcgt acaggccacc actgtccaac tgctgtgatg gtggtgctca tcatggtgtg
     7321 ggatggcatc cctcttccaa tggccgaccg gctatacaca gagctcacag agaatctaaa
     7381 gtcatacaat gggcacccta ccgacagaag atgcaccctc aatgaaaatc gtacctgtac
     7441 atgtcaagga attgatccag agacttgtgg agcttcattc tcttttggct gttcatggag
     7501 tatgtacttt aatggctgta agtttggtag aagcccaagc cccagaagat ttagaattga
     7561 tccaagctct cccttacatg aaaaaaacct tgaagataac ttacagagtt tggctacacg
     7621 attagctcca atttataagc agtatgctcc agtagcttac caaaatcagg tggaatatga
     7681 aaatgttgcc cgagaatgtc ggcttggcag caaggaaggt cgacccttct ctggggtcac
     7741 tgcttgcctg gacttctgtg ctcatcccca cagggacatt cacaacatga ataatggaag
     7801 cactgtggtt tgtaccttaa ctcgagaaga taaccgctct ttgggtgtta ttcctcaaga
     7861 tgagcagctc catgtgctac ctctttataa gctttcagac acagatgagt ttggctccaa
     7921 ggaaggaatg gaagccaaga tcaaatctgg ggccatcgag gtcctggcac cccgccgcaa
     7981 aaaaagaacg tgtttcactc agcctgttcc ccgttctgga aagaagaggg ctgcgatgat
     8041 gacagaggtt cttgcacata agataagggc agtggaaaag aaacctattc cccgaatcaa
     8101 gcggaagaat aactcaacaa caacaaacaa cagtaagcct tcgtcactgc caaccttagg
     8161 gagtaacact gagaccgtgc aacctgaagt aaaaagtgaa accgaacccc attttatctt
     8221 aaaaagttca gacaacacta aaacttattc gctgatgcca tccgctcctc acccagtgaa
     8281 agaggcatct ccaggcttct cctggtcccc gaagactgct tcagccacac cagctccact
     8341 gaagaatgac gcaacagcct catgcgggtt ttcagaaaga agcagcactc cccactgtac
     8401 gatgccttcg ggaagactca gtggtgccaa tgctgcagct gctgatggcc ctggcatttc
     8461 acagcttggc gaagtggctc ctctccccac cctgtctgct cctgtgatgg agcccctcat
     8521 taattctgag ccttccactg gtgtgactga gccgctaacg cctcatcagc caaaccacca
     8581 gccctccttc ctcacctctc ctcaagacct tgcctcttct ccaatggaag aagatgagca
     8641 gcattctgaa gcagatgagc ctccatcaga cgaaccccta tctgatgacc ccctgtcacc
     8701 tgctgaggag aaattgcccc acattgatga gtattggtca gacagtgagc acatcttttt
     8761 ggatgcaaat attggtgggg tggccatcgc acctgctcac ggctcggttt tgattgagtg
     8821 tgcccggcga gagctgcacg ctaccactcc tgttgagcac cccaaccgta atcatccaac
     8881 ccgcctctcc cttgtctttt accagcacaa aaacctaaat aagccccaac atggttttga
     8941 actaaacaag attaagtttg aggctaaaga agctaagaat aagaaaatga aggcctcaga
     9001 gcaaaaagac caggcagcta atgaaggtcc agaacagtcc tctgaagtaa atgaattgaa
     9061 ccaaattcct tctcataaag cattaacatt aacccatgac aatgttgtca ccgtgtcccc
     9121 ttatgctctc acacacgttg cggggcccta taaccattgg gtctgaggcc ggccaggcaa
     9181 aaaagaaaaa gtaagaattc aagggtgggc gcgccgaccc agctttcttg tacaaagtgg
     9241 ttgatatcca gcacagtggc ggccgctcga gtctagaggg cccgcggttc gaatacccat
     9301 acgacgtccc agactacgct tagtaatgat taattaaact agaaattcta ccgggtaggg
     9361 gaggcgcttt tcccaaggca gtctggagta acccacccaa gatctggcct ccgcgccggg
     9421 ttttggcgcc tcccgcgggc gcccccctcc tcacggcgag ccgcgtaaga tctggcctcc
     9481 gcgccgggtt ttggcgcctc ccgcgggcgc ccccctcctc acggcgagcc tagtcgtgag
     9541 gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg
     9601 gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg
     9661 atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag
     9721 tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg
     9781 tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg ccttgaatta
     9841 cttccacctg gctgcagtac gtgattcttg atcccgagct tcgggttgga agtgggtggg
     9901 agagttcgag gccttgcgct taaggagccc cttcgcctcg tgcttgagtt gaggcctggc
     9961 ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt
    10021 tcgataagtc tctagccatt taaaattttt gatgacctgc tgcgacgctt tttttctggc
    10081 aagatagtct tgtaaatgcg ggccaagatc tgcacactgg tatttcggtt tttggggccg
    10141 cgggcggcga cggggcccgt gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag
    10201 cgcggccacc gagaatcgga cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg
    10261 gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag gctggcccgg tcggcaccag
    10321 ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc agggagctca aaatggagga
    10381 cgcggcgctc gggagagcgg gcgggtgagt cacccacaca aaggaaaagg gcctttccgt
    10441 cctcagccgt cgcttcatgt gactccacgg agtaccgggc gccgtccagg cacctcgatt
    10501 agttctcgag cttttggagt acgtcgtctt taggttgggg ggaggggttt tatgcgatgg
    10561 agtttcccca cactgagtgg gtggagactg aagttaggcc agcttggcac ttgatgtaat
    10621 tctccttgga atttgccctt tttgagtttg gatcttggtt cattctcaag cctcagacag
    10681 tggttcaaag tttttttctt ccatttcagg tgtcgtgaag cggccgccat gctagtaaat
    10741 tgtccgctaa attctggccg tttttggctt ttttgttaga cggatccata acttcgtata
    10801 atgtatgcta tacgaagtta tgccaccatg tctagactgg acaagagcaa agtcataaac
    10861 ggagctctgg aattactcaa tggtgtcggt atcgaaggcc tgacgacaag gaaactcgct
    10921 caaaagctgg gagttgagca gcctaccctg tactggcacg tgaagaacaa gcgggccctg
    10981 ctcgatgccc tgccaatcga gatgctggac aggcatcata cccacttctg ccccctggaa
    11041 ggcgagtcat ggcaagactt tctgcggaac aacgccaagt cataccgctg tgctctcctc
    11101 tcacatcgcg acggggctaa agtgcatctc ggcacccgcc caacagagaa acagtacgaa
    11161 accctggaaa atcagctcgc gttcctgtgt cagcaaggct tctccctgga gaacgcactg
    11221 tacgctctgt ccgccgtggg ccactttaca ctgggctgcg tattggagga acaggagcat
    11281 caagtagcaa aagaggaaag agagacacct accaccgatt ctatgccccc acttctgaga
    11341 caagcaattg agctgttcga ccggcaggga gccgaacctg ccttcctttt cggcctggaa
    11401 ctaatcatat gtggcctgga gaaacagcta aagtgcgaaa gcggcgggcc gaccgacgcc
    11461 cttgacgatt ttgacttaga catgctccca gccgatgccc ttgacgactt tgaccttgat
    11521 atgctgcctg ctgacgctct tgacgatttt gaccttgaca tgctccccgg gtaactaagt
    11581 aaggatccgc ggccgcacta gaggaattcc gcccctctcc cccccccccc tccccccccc
    11641 ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt
    11701 attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt
    11761 cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa
    11821 tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac
    11881 cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg
    11941 tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt
    12001 tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca
    12061 gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacatgtgt
    12121 ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg
    12181 aaaaacacga tgataagctt accggtccac catggtgagc aagggcgagg agctgttcac
    12241 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
    12301 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
    12361 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca
    12421 gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
    12481 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
    12541 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
    12601 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
    12661 cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca
    12721 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
    12781 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa
    12841 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
    12901 cactctcggc atggacgagc tgtacaagta agcatgcccg acggcgagga tctcgtcgtg
    12961 acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc
    13021 atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt
    13081 gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc
    13141 gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgatgt
    13201 acaagtaaag cggccgcgac tctagatcat aatcagccat accacatttg tagaggtttt
    13261 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat
    13321 tgttgttgtt tagtccctcc caattcgata tcaagcttat cgcgatagat cctaatcaac
    13381 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
    13441 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
    13501 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
    13561 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
    13621 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
    13681 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
    13741 ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg
    13801 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
    13861 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
    13921 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctgagatc ctttaagacc
    13981 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
    14041 agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg
    14101 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
    14161 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
    14221 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc
    14281 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
    14341 gcccgggtta attaaggaaa gggctagatc attcttgaag acgaaagggc ctcgtgatac
    14401 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
    14461 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
    14521 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
    14581 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
    14641 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
    14701 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
    14761 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
    14821 gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
    14881 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
    14941 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
    15001 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
    15061 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
    15121 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
    15181 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
    15241 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
    15301 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
    15361 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
    15421 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
    15481 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
    15541 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
    15601 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
    15661 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
    15721 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
    15781 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
    15841 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
    15901 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
    15961 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
    16021 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
    16081 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
    16141 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
    16201 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
    16261 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
    16321 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
    16381 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagcaagc
    16441 tcatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
    16501 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctccccgt
    16561 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
    16621 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
    16681 gaattgtgag cggataacaa tttcacacag gaaacagcta tgacatgatt acgaatttca
    16741 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
    16801 cttatcatgt ctggatcaac tggataactc aagctaacca aaatcatccc aaacttccca
    16861 ccccataccc tattaccact gccaattacc tgtggtttca tttactctaa acctgtgatt
    16921 cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag
    16981 tagt
//
