LOCUS       sequence-19821-e         697 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Hoch RV, Soriano P
  TITLE     Context-specific requirements for Fgfr1 signaling through Frs2 and 
            Frs3 during mouse development.
  JOURNAL   Development. 2006 Feb . 133(4):663-73.
  PUBMED    16421190
REFERENCE   2  (bases 1 to 697)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development. 2006 Feb . 133(4):663-73."
COMMENT     Sequence Label: PGKneoF2L2DTA
FEATURES             Location/Qualifiers
     source          1..697
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     9..25
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9..25
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    39..72
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(128..175)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        188..687
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(223..246)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     620..639
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
ORIGIN
        1 gcggccgctc tagaactagt ggatccggaa cccttaatat aacttcgtat aatgtatgct
       61 atacgaagtt attaggtccc tcgacctgca ggaattcgag ctcgcccggg gatcctctag
      121 agtcaacgaa gttcctatac tttctagaga ataggaactt cggaatagga acttcgttaa
      181 ttctaccggg taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc
      241 ccgctgggca cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc
      301 ggtaggcgcc aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc
      361 ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa
      421 gtagcacgtc tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta
      481 ggcctttggg gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg
      541 ggaaggggtg ggtccggggg cgggctcagg ggcgggctca ggggcggggc gggcgcccga
      601 aggtcctccg gaggcccggc attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc
      661 tcctcttcct catctccggg cctttcgacc tgcagcc
//
