LOCUS       sequence-198219-       11154 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral plasmid from FIRE-Cas9 system to express MS2-HP1cs fusion
            protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11154)
  AUTHORS   Braun SMG, Kirkland JG, Chory EJ, Husmann D, Calarco JP, Crabtree GR
  TITLE     Rapid and reversible epigenome editing by endogenous chromatin 
            regulators.
  JOURNAL   Nat Commun. 2017 Sep 15;8(1):560. doi: 10.1038/s41467-017-00644-y.
  PUBMED    28916764
REFERENCE   2  (bases 1 to 11154)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-09-15"; volume: "8(1):560. doi"; pages: " 
            10.1038/s41467-017-00644-y"
COMMENT     Sequence Label: Lv EF1a MS2-HP1cs 2A Hygro
FEATURES             Location/Qualifiers
     source          1..11154
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2687..3865
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2918..3856
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3813..3833
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3872..3881
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3881..4267
                     /codon_start=1
                     /product="bacteriophage MS2 coat protein"
                     /label=MS2-N55K
                     /note="binds with high affinity to a specific stem-loop 
                     structure in the viral RNA (Lim et al., 1994)"
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
                     CSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
                     VKAMQGLLKDGNPIPSAIAANSGIY"
     CDS             4322..4342
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4730..4783
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4787..5806
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     "
     misc_feature    5846..6434
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5899..5919)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6317..6328)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6437..6453)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6438..6454)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6959..7139
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7165..7182)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7171..7395
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7441..7869
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7528..7547)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7738..7759
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7878..7898)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7883..8212
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8063..8198
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8125..8144
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8260..8307
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8326..8700
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8830..8951
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8867..8886)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8921..8940
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9000..9016)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9000..9016)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9013..9035)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9024..9040
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9048..9054,9055..9072,9073..9078))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9093..9114
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9231..9248)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9402..9990)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9482..9501)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10161..10952,10953..11021))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10784..10803
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11022..11126)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta gctagctgca aagatggata aagttttaaa cagagaggaa
     2641 tctttgcagc taatggacct tctaggtctt gaaaggagtg ggaattggct ccggtgcccg
     2701 tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa
     2761 ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg
     2821 gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa
     2881 cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt gtggttcccg
     2941 cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt ccacctggct
     3001 gcagtacgtg attcttgatc ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc
     3061 ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg
     3121 ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct
     3181 agccatttaa aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt
     3241 aaatgcgggc caagatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg
     3301 ggcccgtgcg tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag
     3361 aatcggacgg gggtagtctc aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc
     3421 gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga
     3481 aagatggccg cttcccggcc ctgctgcagg gagctcaaaa tgaaggacgc ggcgctcggg
     3541 agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc
     3601 ttcatgtgac tccacggagt accgggcgcc gtccaggcac ctcgattagt tctcgagctt
     3661 ttggagtacg tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac
     3721 tgagtgggtg gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt
     3781 tgcccttttt gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt
     3841 ttttcttcca tttcaggtgt cgtgacgtac ggccaccatg gcttcaaact ttactcagtt
     3901 cgtgctcgtg gacaatggtg ggacagggga tgtgacagtg gctccttcta atttcgctaa
     3961 tggggtggca gagtggatca gctccaactc acggagccag gcctacaagg tgacatgcag
     4021 cgtcaggcag tctagtgccc agaagagaaa gtataccatc aaggtggagg tccccaaagt
     4081 ggctacccag acagtgggcg gagtcgaact gcctgtcgcc gcttggaggt cctacctgaa
     4141 catggagctc actatcccaa ttttcgctac caattctgac tgtgaactca tcgtgaaggc
     4201 aatgcagggg ctcctcaaag acggtaatcc tatcccttcc gccatcgccg ctaactcagg
     4261 tatctacagc gctggaggag gtggaagcgg aggaggagga agcggaggag gaggtagcgg
     4321 acctaagaaa aagaggaagg tggcggccgc tggatccatg aaggagggtg aaaacaataa
     4381 gcccagggag aaatcagaag gaaacaagag gaaatccagt ttctccaaca gcgctgatga
     4441 tattaaatct aaaaaaaaga gagagcaaag caatgatatc gctcggggct ttgagagagg
     4501 actggaacca gaaaagatca tcggagcaac agattcctgc ggtgacttaa tgttcttaat
     4561 gaaatggaaa gacacagatg aagctgacct ggttcttgca aaagaagcta acgtgaagtg
     4621 tccacagatt gtgatagcat tttatgaaga gagactgacg tggcacgcat atccagagga
     4681 tgcggaaaac aaagaaaaag aaagcgcgaa gagctgtaca ggcagtggag agggcagagg
     4741 aagtctgcta acatgcggtg acgtcgagga gaatcctggc ccaaccatga aaaagcctga
     4801 actcaccgct acctctgtcg agaagtttct gatcgaaaag ttcgacagcg tctccgacct
     4861 gatgcagctc tccgagggcg aagaatctcg ggctttcagc ttcgatgtgg gagggcgtgg
     4921 atatgtcctg cgggtgaata gctgcgccga tggtttctac aaagatcgct atgtttatcg
     4981 gcactttgca tccgccgctc tccctattcc cgaagtgctt gacattgggg agttcagcga
     5041 gagcctgacc tattgcatct cccgccgtgc acagggtgtc accttgcaag acctgcctga
     5101 aaccgaactg cccgctgttc tccagcccgt cgccgaggcc atggatgcca tcgctgccgc
     5161 cgatcttagc cagaccagcg ggttcggccc attcggacct caaggaatcg gtcaatacac
     5221 tacatggcgc gatttcatct gcgctattgc tgatccccat gtgtatcact ggcaaactgt
     5281 gatggacgac accgtcagtg cctccgtcgc ccaggctctc gatgagctga tgctttgggc
     5341 cgaggactgc cccgaagtcc ggcacctcgt gcacgccgat ttcggctcca acaatgtcct
     5401 gaccgacaat ggccgcataa cagccgtcat tgactggagc gaggccatgt tcggggattc
     5461 ccaatacgag gtcgccaaca tcttcttctg gaggccctgg ttggcttgta tggagcagca
     5521 gacccgctac ttcgagcgga ggcatcccga gcttgcagga tctcctcggc tccgggctta
     5581 tatgctccgc attggtcttg accaactcta tcagagcttg gttgacggca atttcgatga
     5641 tgcagcttgg gctcagggtc gctgcgacgc aatcgtccgg tccggagccg ggactgtcgg
     5701 gcgtacacaa atcgcccgca gaagcgctgc cgtctggacc gatggctgtg tggaagtgct
     5761 cgccgatagt ggaaacagac gccccagcac tcgtcctagg gcaaaggatc tgcagtaatg
     5821 agaattcgat atcaagctta tcggtaatca acctctggat tacaaaattt gtgaaagatt
     5881 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     5941 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     6001 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     6061 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     6121 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     6181 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     6241 atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     6301 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     6361 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     6421 ggccgcctcc ccgcatcgat accgtcgacc tcgagaccta gaaaaacatg gagcaatcac
     6481 aagtagcaat acagcagcta ccaatgctga ttgtgcctgg ctagaagcac aagaggagga
     6541 ggaggtgggt tttccagtca cacctcaggt acctttaaga ccaatgactt acaaggcagc
     6601 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
     6661 acgaagacaa gatatccttg atctgtggat ctaccacaca caaggctact tccctgattg
     6721 gcagaactac acaccagggc cagggatcag atatccactg acctttggat ggtgctacaa
     6781 gctagtacca gttgagcaag agaaggtaga agaagccaat gaaggagaga acacccgctt
     6841 gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtat tagagtggag
     6901 gtttgacagc cgcctagcat ttcatcacat ggcccgagag ctgcatccgg actgtactgg
     6961 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     7021 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     7081 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     7141 ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
     7201 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     7261 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     7321 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
     7381 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc
     7441 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     7501 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     7561 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     7621 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     7681 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     7741 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     7801 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     7861 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     7921 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     7981 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     8041 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     8101 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga
     8161 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc
     8221 gggagcttgt atatccattt tcggatctga tcagcacgtg ttgacaatta atcatcggca
     8281 tagtatatcg gcatagtata atacgacaag gtgaggaact aaaccatggc caagttgacc
     8341 agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac
     8401 cggctcgggt tctcccggga cttcgtggag gacgacttcg ccggtgtggt ccgggacgac
     8461 gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc cggacaacac cctggcctgg
     8521 gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt cggaggtcgt gtccacgaac
     8581 ttccgggacg cctccgggcc ggccatgacc gagatcggcg agcagccgtg ggggcgggag
     8641 ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg tggccgagga gcaggactga
     8701 cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     8761 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     8821 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     8881 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     8941 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg
     9001 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     9061 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     9121 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     9181 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     9241 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     9301 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     9361 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     9421 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     9481 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     9541 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     9601 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     9661 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     9721 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     9781 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     9841 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     9901 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     9961 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
    10021 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
    10081 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
    10141 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
    10201 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
    10261 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
    10321 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
    10381 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
    10441 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
    10501 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
    10561 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
    10621 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
    10681 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
    10741 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
    10801 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
    10861 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
    10921 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
    10981 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
    11041 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
    11101 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgac
//
