LOCUS       sequence-19822-e         590 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 590)
  TITLE     Soriano vectors
REFERENCE   2  (bases 1 to 590)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PGKneolox2DTA.2
FEATURES             Location/Qualifiers
     source          1..590
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     59..75
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     59..75
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    89..122
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(187..210)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 gcggccgcct aggagatcta cgcgtcaatt gatgcatccc gggtttaaac taggccgctc
       61 tagaactagt ggatccggaa cccttaatat aacttcgtat aatgtatgct atacgaagtt
      121 attaggtccc tcgacctgca ggaattctac cgggtagggg aggcgctttt cccaaggcag
      181 tctggagcat gcgctttagc agccccgctg ggcacttggc gctacacaag tggcctctgg
      241 cctcgcacac attccacatc caccggtagg cgccaaccgg ctccgttctt tggtggcccc
      301 ttcgcgccac cttctactcc tcccctagtc aggaagttcc cccccgcccc gcagctcgcg
      361 tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca gatggacagc
      421 accgctgagc aatggaagcg ggtaggcctt tggggcagcg gccaatagca gctttgctcc
      481 ttcgctttct gggctcagag gctgggaagg ggtgggtccg ggggcgggct caggggcggg
      541 ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg
//
