LOCUS       sequence-198238-       10501 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral plasmid from FIRE-Cas9 system to express SS18-Frb fusion 
            protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10501)
  AUTHORS   Braun SMG, Kirkland JG, Chory EJ, Husmann D, Calarco JP, Crabtree GR
  TITLE     Rapid and reversible epigenome editing by endogenous chromatin 
            regulators.
  JOURNAL   Nat Commun. 2017 Sep 15;8(1):560. doi: 10.1038/s41467-017-00644-y.
  PUBMED    28916764
REFERENCE   2  (bases 1 to 10501)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-09-15"; volume: "8(1):560. doi"; pages: " 
            10.1038/s41467-017-00644-y"
COMMENT     Sequence Label: Lv EF1a SS18-Frb PGK Puro
FEATURES             Location/Qualifiers
     source          1..10501
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    680..805
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1302..1535
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1720..1764
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2027..2142
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2220..2431
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          2432..3370
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3327..3347
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3392..3658
                     /codon_start=1
                     /product="FKBP-rapamycin binding domain of human FRAP"
                     /label=FRB
                     /translation="MWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETSF
                     NQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISKQ"
     CDS             3689..3955
                     /codon_start=1
                     /product="FKBP-rapamycin binding domain of human FRAP"
                     /label=FRB
                     /translation="MWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETSF
                     NQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISKQ"
     CDS             3962..4003
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     promoter        5333..5832
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5368..5391)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5765..5784
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5853..6452
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5853..5872)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6349..6369
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6469..7057
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6522..6542)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6940..6951)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7264..7897
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8025..8041)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8025..8041)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8038..8060)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8049..8065
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8073..8079,8080..8097,8098..8103))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8118..8139
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8256..8273)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8427..9015)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8507..8526)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9186..9977,9978..10046))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9809..9828
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10047..10151)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    10199..10333
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10236..10255)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10290..10309
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(10501,1..633)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac
       61 acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact
      121 gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa
      181 taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga
      241 gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga
      301 gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc
      361 tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc
      421 ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag
      481 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
      541 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
      601 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
      661 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
      721 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
      781 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
      841 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
      901 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
      961 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     1021 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     1081 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     1141 agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     1201 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     1261 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     1321 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     1381 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1441 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1501 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1561 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1621 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1681 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1741 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     1801 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     1861 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     1921 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     1981 agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccttta aaagaaaagg
     2041 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     2101 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     2161 cagcagagat ccagtttatc gatgaggccc tttcgtcttc actcgaggtg cccgtcagtg
     2221 ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac
     2281 cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg
     2341 cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct
     2401 ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc
     2461 tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct ggctgcagta
     2521 cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga ggccttgcgc
     2581 ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct ggggccgccg
     2641 cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt ctctagccat
     2701 ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc ttgtaaatgc
     2761 gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg acggggcccg
     2821 tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac cgagaatcgg
     2881 acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc cgccgtgtat
     2941 cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag cggaaagatg
     3001 gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct cgggagagcg
     3061 ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg
     3121 tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga gcttttggag
     3181 tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc acactgagtg
     3241 ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg aatttgccct
     3301 ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa gtttttttct
     3361 tccatttcag gtgtcgtgag gatccgcggc catgtggcat gaaggcctgg aagaggcatc
     3421 tcgtttgtac tttggggaaa ggaacgtgaa aggcatgttt gaggtgctgg agcccttgca
     3481 tgctatgatg gaacggggcc cccagactct gaaggaaaca tcctttaatc aggcctatgg
     3541 tcgagattta atggaggccc aagagtggtg caggaagtac atgaaatcag ggaatgtcaa
     3601 ggacctcacc caagcctggg acctctatta tcatgtgttc cgacgaatct caaagcagtt
     3661 cggcggcgga ggtagcggcg gtggatcaat gtggcacgag ggattggagg aagctagcag
     3721 actttatttc ggtgagcgca atgtcaaggg aatgttcgag gtcctcgaac ctctccacgc
     3781 catgatggag agaggacctc aaaccctcaa agagactagt ttcaaccaag cttatggcag
     3841 ggacctgatg gaagctcagg aatggtgtcg caaatatatg aagagcggca acgtgaaaga
     3901 tttgacacag gcttgggatt tgtactacca cgtctttagg aggattagca aacaattcga
     3961 aggtaagcct atccctaacc ctctcctcgg tctcgattct acgggaggcg gatcaggtgg
     4021 cggtagcgcg gccgcaatgt ctgtggcgtt cgcagccccg aggcagcggg gcaagggcga
     4081 aatcacgccc gccgccatcc agaagatgct ggatgaaaac aaccatctta ttcagtgtat
     4141 aatggactat cagaacaaag ggaaggcctc ggagtgctcg cagtatcagc agatattgca
     4201 tacaaacctg gtataccttg ctacaatagc agactctaat caaaatatgc agtctctctt
     4261 accagcaccg cccacacaga ctatgccaat gggtcctgga gggatgagtc agagtggccc
     4321 tccaccccct ccccgctctc acaacatgcc ttcagatgga atggtgggtg ggggccctcc
     4381 tgcaccacac atgcagaacc agatgaacgg ccagatgcct gggcctaacc atatgccaat
     4441 gcagggacct ggacccagtc agctcagcat gacaaacagc tccatgaata tgccttcaag
     4501 tagccatggc tccatgggag gttacaacca ttctgtgccg tcatcccaga gcatgcccgt
     4561 gcagaaccag atgacaatga gtcaggggca gccaatggga aactatggtc ccagaccaaa
     4621 catgaatatg caaccaaatc aagggccgat gatgcaccag cagcctcctt ctcagcagta
     4681 caatatgcca cctggagggg cacagcatta ccaaggacag caggcgccca tggggctgat
     4741 gggccaagtt aaccaaggca gtcacatgat gggccagcga cagatgcctc cctacagacc
     4801 tccgcaacag ggcccaccac agcagtactc aggccaggaa gactattatg gggaccaata
     4861 cagtcatggt ggacaaggtc ctccagaagg catgaaccag caatattacc ctgatggtca
     4921 taatgattac ggttatcagc aaccgtcgta tcctgaacaa ggctacgata ggccttatga
     4981 ggattcctca caacattact acgaaggagg aaactcccag tatggccaac agcaagacgc
     5041 ttaccaggga ccacctccac agcaaggata cccaccccag cagcagcagt acccgggaca
     5101 gcagggatac ccagggcagc agcagagcta tggtccttcg cagggcggtc caggtcctca
     5161 gtatcctaat tatcctcagg gtcaaggtca gcagtatggg ggctatagac caacacagcc
     5221 aggaccaccc cagccacccc agcagaggcc ttatgggtac gaccagggac agtatggaaa
     5281 ttaccagcag tgagccgcgc cggctctaga tcgcgaacgc gtgaattcta ccgggtaggg
     5341 gaggcgcttt tcccaaggca gtctggagca tgcgctttag cagccccgct gggcacttgg
     5401 cgctacacaa gtggcctctg gcctcgcaca cattccacat ccaccggtag gcgccaaccg
     5461 gctccgttct ttggtggccc cttcgcgcca ccttctactc ctcccctagt caggaagttc
     5521 ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact
     5581 agtctcgtgc agatggacag caccgctgag caatggaagc gggtaggcct ttggggcagc
     5641 ggccaatagc agctttgctc cttcgctttc tgggctcaga ggctgggaag gggtgggtcc
     5701 gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc
     5761 ccggcattct gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct
     5821 ccgggccttt cgacctgcag cccaagctta ccatgaccga gtacaagccc acggtgcgcc
     5881 tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact
     5941 accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc
     6001 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg
     6061 gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg
     6121 agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg
     6181 aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg
     6241 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg
     6301 cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct
     6361 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca
     6421 cctggtgcat gacccgcaag cccggtgcct gacgggcgcg tctggaacaa tcaacctctg
     6481 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     6541 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     6601 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     6661 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     6721 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     6781 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     6841 aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc
     6901 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     6961 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     7021 cagacgagtc ggatctccct ttgggccgcc tccccgcctg gaattaattc tgcagtcgag
     7081 acctagaaaa acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg
     7141 cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct caggtacctt
     7201 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaagaggg
     7261 gactggaagg gctaattcac tcccaacgaa gacaagatat ccttgatctg tggatctacc
     7321 acacacaagg ctacttccct gattagcaga actacacacc agggccaggg gtcagatatc
     7381 cactgacctt tggatggtgc tacaagctag taccagttga gccagataag gtagaagagg
     7441 ccaataaagg agagaacacc agcttgttac accctgtgag cctgcatggg atggatgacc
     7501 cggagagaga agtgttagag tggaggtttg acagccgcct agcatttcat cacgtggccc
     7561 gagagctgca tccggagtac ttcaagaact gctgatatcg agcttgctac aagggacttt
     7621 ccgctgggga ctttccaggg aggcgtggcc tgggcgggac tggggagtgg cgagccctca
     7681 gatcctgcat ataagcagct gctttttgcc tgtactgggt ctctctggtt agaccagatc
     7741 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     7801 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     7861 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt
     7921 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggcc ttgacattgc
     7981 tagcgtttac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct
     8041 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     8101 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     8161 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     8221 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     8281 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     8341 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     8401 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     8461 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     8521 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     8581 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     8641 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     8701 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     8761 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     8821 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     8881 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     8941 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     9001 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     9061 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     9121 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     9181 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     9241 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     9301 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     9361 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     9421 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     9481 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     9541 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     9601 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     9661 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     9721 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     9781 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     9841 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     9901 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     9961 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
    10021 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
    10081 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
    10141 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatcaa
    10201 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
    10261 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
    10321 tcatgtctgg atcaactgga taactcaagc taaccaaaat catcccaaac ttcccacccc
    10381 ataccctatt accactgcca attacctgtg gtttcattta ctctaaacct gtgattcctc
    10441 tgaattattt tcattttaaa gaaattgtat ttgttaaata tgtactacaa acttagtagt
    10501 t
//
