LOCUS       sequence-198246-        4140 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BphP1-PpsR2 reporter plasmid; PBr_crtE-sfGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4140)
  AUTHORS   Ong NTX, Olson EJ, Tabor JJ
  TITLE     Engineering an E. coli near-infrared light sensor.
  JOURNAL   ACS Synth Biol. 2017 Nov 1. doi: 10.1021/acssynbio.7b00289.
  PUBMED    29091422
REFERENCE   2  (bases 1 to 4140)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2017 Nov 1. doi: 10.1021/acssynbio.7b00289."
COMMENT     Sequence Label: pNO4
FEATURES             Location/Qualifiers
     source          1..4140
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             86..799
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     misc_feature    826..846
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      847..904
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(928..945)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1207..2157)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      2205..2427
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(2919..3013)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(3043..3834,3835..3903))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3666..3685
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3904..4008)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4073..4091
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(4095..4138)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
ORIGIN
        1 gacgcaggaa atgtctagca tgatttacag tataggtgta agctgtaatt tacatgtaaa
       61 gaaataaaaa gggaggttac aacctatgcg taaaggcgaa gagctgttca ctggtgtcgt
      121 ccctattctg gtggaactgg atggtgatgt caacggtcat aagttttccg tgcgtggcga
      181 gggtgaaggt gacgcaacta atggtaaact gacgctgaag ttcatctgta ctactggtaa
      241 actgccggta ccttggccga ctctggtaac gacgctgact tatggtgttc agtgctttgc
      301 tcgttatccg gaccatatga agcagcatga cttcttcaag tccgccatgc cggaaggcta
      361 tgtgcaggaa cgcacgattt cctttaagga tgacggcacg tacaaaacgc gtgcggaagt
      421 gaaatttgaa ggcgataccc tggtaaaccg cattgagctg aaaggcattg actttaaaga
      481 agacggcaat atcctgggcc ataagctgga atacaatttt aacagccaca atgtttacat
      541 caccgccgat aaacaaaaaa atggcattaa agcgaatttt aaaattcgcc acaacgtgga
      601 ggatggcagc gtgcagctgg ctgatcacta ccagcaaaac actccaatcg gtgatggtcc
      661 tgttctgctg ccagacaatc actatctgag cacgcaaagc gttctgtcta aagatccgaa
      721 cgagaaacgc gatcatatgg ttctgctgga gttcgtaacc gcagcgggca tcacgcatgg
      781 tatggatgaa ctgtacaaat gatgagcccg tatttcgcgt aaggatacta gtagcggccg
      841 ctgcagtccg gcaaaaaagg gcaaggtgtc accaccctgc cctttttctt taaaaccgaa
      901 aagattactt cgcgttatgc aggcttcctc gctcactgac tcgctgcgct cggtcgttcg
      961 gctgcggcga gcggtatcag ctcactcaaa ggcggtaatc tcgaggttac attgtcgatc
     1021 tgttcatggt gaacagcttt gaatgcacca aaaactcgta aaagctctga tgtatctatc
     1081 ttttttacac cgttttcatc tgtgcatatg gacagttttc cctttgatat gtaacggtga
     1141 acagttgttc tacttttgtt tgttagtctt gatgcttcac tgatagatac aagagccata
     1201 agaacctcag atccttccgt atttagccag tatgttctct agtgtggttc gttgtttttg
     1261 cgtgagccat gagaacgaac cattgagatc atacttactt tgcatgtcac tcaaaaattt
     1321 tgcctcaaaa ctggtgagct gaatttttgc agttaaagca tcgtgtagtg tttttcttag
     1381 tccgttatgt aggtaggaat ctgatgtaat ggttgttggt attttgtcac cattcatttt
     1441 tatctggttg ttctcaagtt cggttacgag atccatttgt ctatctagtt caacttggaa
     1501 aatcaacgta tcagtcgggc ggcctcgctt atcaaccacc aatttcatat tgctgtaagt
     1561 gtttaaatct ttacttattg gtttcaaaac ccattggtta agccttttaa actcatggta
     1621 gttattttca agcattaaca tgaacttaaa ttcatcaagg ctaatctcta tatttgcctt
     1681 gtgagttttc ttttgtgtta gttcttttaa taaccactca taaatcctca tagagtattt
     1741 gttttcaaaa gacttaacat gttccagatt atattttatg aattttttta actggaaaag
     1801 ataaggcaat atctcttcac taaaaactaa ttctaatttt tcgcttgaga acttggcata
     1861 gtttgtccac tggaaaatct caaagccttt aaccaaagga ttcctgattt ccacagttct
     1921 cgtcatcagc tctctggttg ctttagctaa tacaccataa gcattttccc tactgatgtt
     1981 catcatctga gcgtattggt tataagtgaa cgataccgtc cgttctttcc ttgtagggtt
     2041 ttcaatcgtg gggttgagta gtgccacaca gcataaaatt agcttggttt catgctccgt
     2101 taagtcatag cgactaatcg ctagttcatt tgctttgaaa acaactaatt cagacataca
     2161 tctcaattgg tctaggtgat tttaatcact ataccaattg agatgggcta gtcaatgata
     2221 attacatgtc cttttccttt gagttgtggg tatctgtaaa ttctgctaga cctttgctgg
     2281 aaaacttgta aattctgcta gaccctctgt aaattccgct agacctttgt gtgttttttt
     2341 tgtttatatt caagtggtta taatttatag aataaagaaa gaataaaaaa agataaaaag
     2401 aatagatccc agccctgtgt ataactcact actttagtca gttccgcagt attacaaaag
     2461 gatgtcgcaa acgctgtttg ctcctctaca aaacagacct taaaacccta aaggcttaag
     2521 tagcaccctc gcaagctcgg gcaaatcgct gaatattcct tttgtctccg accatcaggc
     2581 acctgagtcg ctgtcttttt cgtgacattc agttcgctgc gctcacggct ctggcagtga
     2641 atgggggtaa atggcactac aggcgccttt tatggattca tgcaaggaaa ctacccataa
     2701 tacaagaaaa gcccgtcacg ggcttctcag ggcgttttat ggcgggtctg ctatgtggtg
     2761 ctatctgact ttttgctgtt cagcagttcc tgccctctga ttttccagtc tgaccacttc
     2821 ggattatccc gtgacaggtc attcagactg gctaatgcac ccagtaaggc agcggtatca
     2881 tcaacaggct tacccgtctt actgtcccta gtgcttggat tctcaccaat aaaaaacgcc
     2941 cggcggcaac cgagcgttct gaacaaatcc agatggagtt ctgaggtcat tactggatct
     3001 atcaacagga gtccaagcga gctcgtaaac ttggtctgac agttaccaat gcttaatcag
     3061 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     3121 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     3181 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     3241 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     3301 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     3361 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     3421 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     3481 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     3541 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     3601 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     3661 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     3721 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     3781 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     3841 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     3901 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     3961 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttcccat
     4021 ggtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     4081 tatcacgagg cagaatttca gataaaaaaa atccttagct ttcgctaagg atgatttctg
//
