LOCUS       sequence-198257-       15593 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PiggyBac construct with doxycyline inducible TRE-tight promoter 
            expression of TMP inducible DDdCas9VP192-P300core-p65-HSF1 activator
            followed by T2A-EGFP as a reporter and IRES-Neo as selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15593)
  AUTHORS   Weltner J, Balboa D, Katayama S, Bespalov M, Krjutskov K, Jouhilahti
            EM, Trokovic R, Kere J, Otonkoski T
  TITLE     Human pluripotent reprogramming with CRISPR activators.
  JOURNAL   Nat Commun. 2018 Jul 6;9(1):2643. doi: 10.1038/s41467-018-05067-x.
  PUBMED    29980666
REFERENCE   2  (bases 1 to 15593)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-07-6"; volume: "9(1):2643. doi"; pages: " 
            10.1038/s41467-018-05067-x"
COMMENT     Sequence Label: PB-tight-DDdCas9VPPH-T2A-GFP-IRES-Neo
FEATURES             Location/Qualifiers
     source          1..15593
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..22
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(37..42,43..60,61..67)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    75..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     99..115
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     134..154
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        136..154
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     repeat_region   205..267
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     CDS             557..667
                     /codon_start=1
                     /product="3 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP48
                     /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML"
     promoter        704..1018
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    712..730
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    748..766
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    783..801
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    819..837
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    855..873
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    890..908
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    926..944
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     991..1015
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1218..1237
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1277..1753
                     /codon_start=1
                     /gene="folA"
                     /product="E. coli dihydrofolate reductase"
                     /label=ecDHFR
                     /translation="MISLIAALAVDYVIGMENAMPWNLPADLAWFKRNTLNKPVIMGRH
                     TWESIGRPLPGRKNIILSSQPSTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVIEQFL
                     PKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     CDS             1778..1804
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1808..1828
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(1838..1861,1862..1864,1865..4420,4421..4423,
                     4424..5938)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the N863A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(1838..1861,1862..1864,1865..4351,4352..4354,
                     4355..5938)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5942..5962
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5999..6109
                     /codon_start=1
                     /product="3 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP48
                     /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML"
     CDS             6161..6271
                     /codon_start=1
                     /product="3 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP48
                     /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML"
     CDS             6323..6433
                     /codon_start=1
                     /product="3 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP48
                     /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML"
     CDS             6485..6595
                     /codon_start=1
                     /product="3 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP48
                     /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML"
     CDS             6644..8494
                     /codon_start=1
                     /gene="EP300"
                     /product="catalytic histone acetyltransferase core of the 
                     human E1A-associated protein p300 (Delvecchio et al., 
                     2013)"
                     /label=p300 core
                     /translation="IFKPEELRQALMPTLEALYRQDPESLPFRQPVDPQLLGIPDYFDI
                     VKSPMDLSTIKRKLDTGQYQEPWQYVDDIWLMFNNAWLYNRKTSRVYKYCSKLSEVFEQ
                     EIDPVMQSLGYCCGRKLEFSPQTLCCYGKQLCTIPRDATYYSYQNRYHFCEKCFNEIQG
                     ESVSLGDDPSQPQTTINKEQFSKRKNDTLDPELFVECTECGRKMHQICVLHHEIIWPAG
                     FVCDGCLKKSARTRKENKFSAKRLPSTRLGTFLENRVNDFLRRQNHPESGEVTVRVVHA
                     SDKTVEVKPGMKARFVDSGEMAESFPYRTKALFAFEEIDGVDLCFFGMHVQEYGSDCPP
                     PNQRRVYISYLDSVHFFRPKCLRTAVYHEILIGYLEYVKKLGYTTGHIWACPPSEGDDY
                     IFHCHPPDQKIPKPKRLQEWYKKMLDKAVSERIVHDYKDIFKQATEDRLTSAKELPYFE
                     GDFWPNVLEESIKELEQEEEERKREENTSNESTDVTKGDSKNAKKKNNKKTSKNKSSLS
                     RGNKKKPGMPNVSNDLSQKLYATMEKHKEVFFVIRLIAGPAANSLPPIVDPDPLIPCDL
                     MDGRDAFLTLARDKHLEFSSLRRAQWSTMCMLVELHTQSQD"
     regulatory      8258..8267
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             9098..9469
                     /codon_start=1
                     /product="C-terminal activation domain from the human heat 
                     shock transcription factor HSF1"
                     /label=HSF1
                     /translation="GFSVDTSALLDLFSPSVTVPDMSLPDLDSSLASIQELLSPQEPPR
                     PPEAENSSPDSGKQLVHYTAQPLFLLDPGSVDTGSNDLPVLFELGEGSYFSEGDGFAED
                     PTISLLTGSEPPKAKDPTVS"
     CDS             9503..9556
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(9563..9565,9566..9568,9569..10282)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(9563..9565,9566..9568,9569..10279)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(9869..9888)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     10216..10237
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    join(10304..10878,10879..10881,10882..10890)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(10471..10488)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     10698..10717
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             10891..11685
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MAEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(10945..10964)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     11555..11574
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(11987..12004)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    11993..12217
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(12264..12280)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    12288..12304
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12312..12318,12319..12336,12337..12342))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12357..12378
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     protein_bind    complement(12437..12561)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     repeat_region   12851..12885
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(12914..12933)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(12915..12933)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(12943..12960)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(12943..12959)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12952..12974)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      13101..13556
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(13188..13207)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     13398..13419
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        13582..13686
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(13687..13755,13756..14547)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(13905..13924)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      14718..15306
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     15207..15226
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     15460..15477
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
       61 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
      121 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggatt aattaagcgg
      181 ccgcatacgc gtatactaga ttaaccctag aaagataatc atattgtgac gtacgttaaa
      241 gataatcatg cgtaaaattg acgcatgtgt tttatcggtc tgtatatcga ggtttattta
      301 ttaatttgaa tagatattaa gttttattat atttacactt acatactaat aataaattca
      361 acaaacaatt tatttatgtt tatttattta ttaaaaaaaa acaaaaactc aaaatttctt
      421 ctataaagta acaaaacttt taactagaat tgagctgttc gaccggcagg gagccgaacc
      481 tgccttcctt ttcggcctgg aactaatcat atgtggcctg gagaaacagc taaagtgcga
      541 aagcggcggg ccggccgacg cccttgacga ttttgactta gacatgctcc cagccgatgc
      601 ccttgacgac tttgaccttg atatgctgcc tgctgacgct cttgacgatt ttgaccttga
      661 catgctcccc gggtaactag aattgtaaat gcattcaaac gtcgagttta ctccctatca
      721 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacga tgtcgagttt
      781 actccctatc agtgatagag aacgtatgtc gagtttactc cctatcagtg atagagaacg
      841 tatgtcgagt ttactcccta tcagtgatag agaacgtatg tcgagtttat ccctatcagt
      901 gatagagaac gtatgtcgag tttactccct atcagtgata gagaacgtat gtcgaggtag
      961 gcgtgtacgg tgggaggcct atataagcag agctcgttta gtgaaccgtc agatcgcctg
     1021 gagaaggatc cgcggccgcg ccggctctag atcgcgaacg cgtgaattgt aaatgcattc
     1081 aaacgtcgac gataaaataa aagattttat ttagtctcca gaaaaagggg ggaatgaaag
     1141 accccacctg taggtttggc aagctagagc ctctgctaac catgttcatg ccttcttctt
     1201 tttcctacag ctcctgggca acgtgctggt tattgtgctg tctcatcatt ttggcaaaga
     1261 attgggatcc gccaccatga tcagtctgat tgcggcgtta gcggtagatt acgttatcgg
     1321 catggaaaac gccatgccgt ggaacctgcc tgccgatctc gcctggttta aacgcaacac
     1381 cttaaataaa cccgtgatta tgggccgcca tacctgggaa tcaatcggtc gtccgttgcc
     1441 aggacgcaaa aatattatcc tcagcagtca accgagtacg gacgatcgcg taacgtgggt
     1501 gaagtcggtg gatgaagcca tcgcggcgtg tggtgacgta ccagaaatca tggtgattgg
     1561 cggcggtcgc gttattgaac agttcttgcc aaaagcgcaa aaactgtatc tgacgcatat
     1621 cgacgcagaa gtggaaggcg acacccattt cccggattac gagccggatg actgggaatc
     1681 ggtattcagc gaattccacg atgctgatgc gcagaactct cacagctatt gctttgagat
     1741 tctggagcgg cgaggctccg gaaccggtgc caccatgtac ccatacgatg ttccagatta
     1801 cgcttcgccg aagaaaaagc gcaaggtcga agcgtccgac aagaagtaca gcatcggcct
     1861 ggccatcggc accaactctg tgggctgggc cgtgatcacc gacgagtaca aggtgcccag
     1921 caagaaattc aaggtgctgg gcaacaccga ccggcacagc atcaagaaga acctgatcgg
     1981 agccctgctg ttcgacagcg gcgaaacagc cgaggccacc cggctgaaga gaaccgccag
     2041 aagaagatac accagacgga agaaccggat ctgctatctg caagagatct tcagcaacga
     2101 gatggccaag gtggacgaca gcttcttcca cagactggaa gagtccttcc tggtggaaga
     2161 ggataagaag cacgagcggc accccatctt cggcaacatc gtggacgagg tggcctacca
     2221 cgagaagtac cccaccatct accacctgag aaagaaactg gtggacagca ccgacaaggc
     2281 cgacctgcgg ctgatctatc tggccctggc ccacatgatc aagttccggg gccacttcct
     2341 gatcgagggc gacctgaacc ccgacaacag cgacgtggac aagctgttca tccagctggt
     2401 gcagacctac aaccagctgt tcgaggaaaa ccccatcaac gccagcggcg tggacgccaa
     2461 ggccatcctg tctgccagac tgagcaagag cagacggctg gaaaatctga tcgcccagct
     2521 gcccggcgag aagaagaatg gcctgttcgg caacctgatt gccctgagcc tgggcctgac
     2581 ccccaacttc aagagcaact tcgacctggc cgaggatgcc aaactgcagc tgagcaagga
     2641 cacctacgac gacgacctgg acaacctgct ggcccagatc ggcgaccagt acgccgacct
     2701 gtttctggcc gccaagaacc tgtccgacgc catcctgctg agcgacatcc tgagagtgaa
     2761 caccgagatc accaaggccc ccctgagcgc ctctatgatc aagagatacg acgagcacca
     2821 ccaggacctg accctgctga aagctctcgt gcggcagcag ctgcctgaga agtacaaaga
     2881 gattttcttc gaccagagca agaacggcta cgccggctac attgacggcg gagccagcca
     2941 ggaagagttc tacaagttca tcaagcccat cctggaaaag atggacggca ccgaggaact
     3001 gctcgtgaag ctgaacagag aggacctgct gcggaagcag cggaccttcg acaacggcag
     3061 catcccccac cagatccacc tgggagagct gcacgccatt ctgcggcggc aggaagattt
     3121 ttacccattc ctgaaggaca accgggaaaa gatcgagaag atcctgacct tccgcatccc
     3181 ctactacgtg ggccctctgg ccaggggaaa cagcagattc gcctggatga ccagaaagag
     3241 cgaggaaacc atcaccccct ggaacttcga ggaagtggtg gacaagggcg cttccgccca
     3301 gagcttcatc gagcggatga ccaacttcga taagaacctg cccaacgaga aggtgctgcc
     3361 caagcacagc ctgctgtacg agtacttcac cgtgtataac gagctgacca aagtgaaata
     3421 cgtgaccgag ggaatgagaa agcccgcctt cctgagcggc gagcagaaaa aggccatcgt
     3481 ggacctgctg ttcaagacca accggaaagt gaccgtgaag cagctgaaag aggactactt
     3541 caagaaaatc gagtgcttcg actccgtgga aatctccggc gtggaagatc ggttcaacgc
     3601 ctccctgggc acataccacg atctgctgaa aattatcaag gacaaggact tcctggacaa
     3661 tgaggaaaac gaggacattc tggaagatat cgtgctgacc ctgacactgt ttgaggacag
     3721 agagatgatc gaggaacggc tgaaaaccta tgcccacctg ttcgacgaca aagtgatgaa
     3781 gcagctgaag cggcggagat acaccggctg gggcaggctg agccggaagc tgatcaacgg
     3841 catccgggac aagcagtccg gcaagacaat cctggatttc ctgaagtccg acggcttcgc
     3901 caacagaaac ttcatgcagc tgatccacga cgacagcctg acctttaaag aggacatcca
     3961 gaaagcccag gtgtccggcc agggcgatag cctgcacgag cacattgcca atctggccgg
     4021 cagccccgcc attaagaagg gcatcctgca gacagtgaag gtggtggacg agctcgtgaa
     4081 agtgatgggc cggcacaagc ccgagaacat cgtgatcgaa atggccagag agaaccagac
     4141 cacccagaag ggacagaaga acagccgcga gagaatgaag cggatcgaag agggcatcaa
     4201 agagctgggc agccagatcc tgaaagaaca ccccgtggaa aacacccagc tgcagaacga
     4261 gaagctgtac ctgtactacc tgcagaatgg gcgggatatg tacgtggacc aggaactgga
     4321 catcaaccgg ctgtccgact acgatgtcga cgccatcgtg cctcagagct ttctgaagga
     4381 cgactccatc gacaacaagg tgctgaccag aagcgacaag aaccggggca agagcgacaa
     4441 cgtgccctcc gaagaggtcg tgaagaagat gaagaactac tggcggcagc tgctgaacgc
     4501 caagctgatt acccagagaa agttcgacaa tctgaccaag gccgagagag gcggcctgag
     4561 cgaactggat aaggccggct tcatcaagag acagctggtg gaaacccggc agatcacaaa
     4621 gcacgtggca cagatcctgg actcccggat gaacactaag tacgacgaga atgacaagct
     4681 gatccgggaa gtgaaagtga tcaccctgaa gtccaagctg gtgtccgatt tccggaagga
     4741 tttccagttt tacaaagtgc gcgagatcaa caactaccac cacgcccacg acgcctacct
     4801 gaacgccgtc gtgggaaccg ccctgatcaa aaagtaccct aagctggaaa gcgagttcgt
     4861 gtacggcgac tacaaggtgt acgacgtgcg gaagatgatc gccaagagcg agcaggaaat
     4921 cggcaaggct accgccaagt acttcttcta cagcaacatc atgaactttt tcaagaccga
     4981 gattaccctg gccaacggcg agatccggaa gcggcctctg atcgagacaa acggcgaaac
     5041 cggggagatc gtgtgggata agggccggga ttttgccacc gtgcggaaag tgctgagcat
     5101 gccccaagtg aatatcgtga aaaagaccga ggtgcagaca ggcggcttca gcaaagagtc
     5161 tatcctgccc aagaggaaca gcgataagct gatcgccaga aagaaggact gggaccctaa
     5221 gaagtacggc ggcttcgaca gccccaccgt ggcctattct gtgctggtgg tggccaaagt
     5281 ggaaaagggc aagtccaaga aactgaagag tgtgaaagag ctgctgggga tcaccatcat
     5341 ggaaagaagc agcttcgaga agaatcccat cgactttctg gaagccaagg gctacaaaga
     5401 agtgaaaaag gacctgatca tcaagctgcc taagtactcc ctgttcgagc tggaaaacgg
     5461 ccggaagaga atgctggcct ctgccggcga actgcagaag ggaaacgaac tggccctgcc
     5521 ctccaaatat gtgaacttcc tgtacctggc cagccactat gagaagctga agggctcccc
     5581 cgaggataat gagcagaaac agctgtttgt ggaacagcac aagcactacc tggacgagat
     5641 catcgagcag atcagcgagt tctccaagag agtgatcctg gccgacgcta atctggacaa
     5701 agtgctgtcc gcctacaaca agcaccggga taagcccatc agagagcagg ccgagaatat
     5761 catccacctg tttaccctga ccaatctggg agcccctgcc gccttcaagt actttgacac
     5821 caccatcgac cggaagaggt acaccagcac caaagaggtg ctggacgcca ccctgatcca
     5881 ccagagcatc accggcctgt acgagacacg gatcgacctg tctcagctgg gaggcgacag
     5941 ccccaagaag aagagaaagg tggaggccag cctcgagtgc gaaagcggcg ggccggccga
     6001 cgcccttgac gattttgact tagacatgct cccagccgat gcccttgacg actttgacct
     6061 tgatatgctg cctgctgacg ctcttgacga ttttgacctt gacatgctcc ccgggtcact
     6121 acccagcgct agcgtcgagt gcgaaagcgg cgggccggcc gacgcccttg acgattttga
     6181 cttagacatg ctcccagccg atgcccttga cgactttgac cttgatatgc tgcctgctga
     6241 cgctcttgac gattttgacc ttgacatgct ccccgggtca ctacccagcg ctagcgtcga
     6301 gtgcgaaagc ggcgggccgg ccgacgccct tgacgatttt gacttagaca tgctcccagc
     6361 cgatgccctt gacgactttg accttgatat gctgcctgct gacgctcttg acgattttga
     6421 ccttgacatg ctccccgggt cactacccag cgctagcgtc gagtgcgaaa gcggcgggcc
     6481 ggccgacgcc cttgacgatt ttgacttaga catgctccca gccgatgccc ttgacgactt
     6541 tgaccttgat atgctgcctg ctgacgctct tgacgatttt gaccttgaca tgctccccgg
     6601 gtcactaccc agcgctagcg tcgagttcga aggcagcggc gggattttca aaccagaaga
     6661 actacgacag gcactgatgc caactttgga ggcactttac cgtcaggatc cagaatccct
     6721 tccctttcgt caacctgtgg accctcagct tttaggaatc cctgattact ttgatattgt
     6781 gaagagcccc atggatcttt ctaccattaa gaggaagtta gacactggac agtatcagga
     6841 gccctggcag tatgtcgatg atatttggct tatgttcaat aatgcctggt tatataaccg
     6901 gaaaacatca cgggtataca aatactgctc caagctctct gaggtctttg aacaagaaat
     6961 tgacccagtg atgcaaagcc ttggatactg ttgtggcaga aagttggagt tctctccaca
     7021 gacactgtgt tgctacggca aacagttgtg cacaatacct cgtgatgcca cttattacag
     7081 ttaccagaac aggtatcatt tctgtgagaa gtgtttcaat gagatccaag gggagagcgt
     7141 ttctttgggg gatgaccctt cccagcctca aactacaata aataaagaac aattttccaa
     7201 gagaaaaaat gacacactgg atcctgaact gtttgttgaa tgtacagagt gcggaagaaa
     7261 gatgcatcag atctgtgtcc ttcaccatga gatcatctgg cctgctggat tcgtctgtga
     7321 tggctgttta aagaaaagtg cacgaactag gaaagaaaat aagttttctg ctaaaaggtt
     7381 gccatctacc agacttggca cctttctaga gaatcgtgtg aatgactttc tgaggcgaca
     7441 gaatcaccct gagtcaggag aggtcactgt tagagtagtt catgcttctg acaaaaccgt
     7501 ggaagtaaaa ccaggcatga aagcaaggtt tgtggacagt ggagagatgg cagaatcctt
     7561 tccataccga accaaagccc tctttgcctt tgaagaaatt gatggtgttg acctgtgctt
     7621 ctttggcatg catgttcaag agtatggctc tgactgccct ccacccaacc agaggagagt
     7681 atacatatct tacctcgata gtgttcattt cttccgtcct aaatgcttga ggactgcagt
     7741 ctatcatgaa atcctaattg gatatttaga atatgtcaag aaattaggtt acacaacagg
     7801 gcatatttgg gcatgtccac caagtgaggg agatgattat atcttccatt gccatcctcc
     7861 tgaccagaag atacccaagc ccaagcgact gcaggaatgg tacaaaaaaa tgcttgacaa
     7921 ggctgtatca gagcgtattg tccatgacta caaggatatt tttaaacaag ctactgaaga
     7981 tagattaaca agtgcaaagg aattgcctta tttcgagggt gatttctggc ccaatgttct
     8041 ggaagaaagc attaaggaac tggaacagga ggaagaagag agaaaacgag aggaaaacac
     8101 cagcaatgaa agcacagatg tgaccaaggg agacagcaaa aatgctaaaa agaagaataa
     8161 taagaaaacc agcaaaaata agagcagcct gagtaggggc aacaagaaga aacccgggat
     8221 gcccaatgta tctaacgacc tctcacagaa actatatgcc accatggaga agcataaaga
     8281 ggtcttcttt gtgatccgcc tcattgctgg ccctgctgcc aactccctgc ctcccattgt
     8341 tgatcctgat cctctcatcc cctgcgatct gatggatggt cgggatgcgt ttctcacgct
     8401 ggcaagggac aagcacctgg agttctcttc actccgaaga gcccagtggt ccaccatgtg
     8461 catgctggtg gagctgcaca cgcagagcca ggacgtcgac actagcgtcg agttcgaagg
     8521 cagcggcggg ccttcagggc agatcagcaa ccaggccctg gctctggccc ctagctccgc
     8581 tccagtgctg gcccagacta tggtgccctc tagtgctatg gtgcctctgg cccagccacc
     8641 tgctccagcc cctgtgctga ccccaggacc accccagtca ctgagcgctc cagtgcccaa
     8701 gtctacacag gccggcgagg ggactctgag tgaagctctg ctgcacctgc agttcgacgc
     8761 tgatgaggac ctgggagctc tgctggggaa cagcaccgat cccggagtgt tcacagatct
     8821 ggcctccgtg gacaactctg agtttcagca gctgctgaat cagggcgtgt ccatgtctca
     8881 tagtacagcc gaaccaatgc tgatggagta ccccgaagcc attacccggc tggtgaccag
     8941 cagccagcgg ccccccgacc ccgctccaac tcccctggga accagcggcc tgcctaatgg
     9001 gctgtccgga gatgaagact tctcaagcat cgctgatatg gactttagtg ccctgctgtc
     9061 acagatttcc tctagtgggc agggaggagg tggaagcggc ttcagcgtgg acaccagtgc
     9121 cctgctggac ctgttcagcc cctcggtgac cgtgcccgac atgagcctgc ctgaccttga
     9181 cagcagcctg gccagtatcc aagagctcct gtctccccag gagcccccca ggcctcccga
     9241 ggcagagaac agcagcccgg attcagggaa gcagctggtg cactacacag cgcagccgct
     9301 gttcctgctg gaccccggct ccgtggacac cgggagcaac gacctgccgg tgctgtttga
     9361 gctgggagag ggctcctact tctccgaagg ggacggcttc gccgaggacc ccaccatctc
     9421 cctgctgaca ggctcggagc ctcccaaagc caaggacccc actgtctcct ctagagtcga
     9481 cactagcgtc gacggctccg gagagggcag aggaagtctg ctaacatgcg gtgacgtcga
     9541 ggagaatcct ggcccaatcg atatggtgag caagggcgag gagctgttca ccggggtggt
     9601 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     9661 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
     9721 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag
     9781 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     9841 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     9901 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     9961 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
    10021 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga
    10081 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
    10141 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa
    10201 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
    10261 catggacgag ctgtacaagt aatttaaaga attccgcccc ccccccctct ccctcccccc
    10321 cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt
    10381 tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct
    10441 tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga
    10501 atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga
    10561 ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac
    10621 gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag
    10681 ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc
    10741 agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg
    10801 tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt
    10861 gaaaaacacg atgataagct tgccacaacc atggctgaac aagatggatt gcacgcaggt
    10921 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
    10981 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
    11041 accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg
    11101 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
    11161 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
    11221 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
    11281 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
    11341 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
    11401 ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
    11461 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
    11521 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
    11581 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
    11641 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg atcggctaga
    11701 gtcgacccgg acgggacgct cctgcgcctg atacagaacg aattgcttgc aggcatctca
    11761 tgagtgtgtc ttcccgtttt ccgcctgagg tcactgcgtg gatggagcgc tggcgcctgc
    11821 tgcgcgacgg cgagctgctc accacccact cgagggctgc agcgctgcag aggccgagtg
    11881 cagaactgct ccaaagggac ctcaaggctt tccgagggac actaggctga ctccatcgag
    11941 ccagtgtaga gataagctta tcgatgatct agagctcgct gatcagcctc gactgtgcct
    12001 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
    12061 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
    12121 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
    12181 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaacctgc
    12241 agcccaagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
    12301 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
    12361 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
    12421 tcgtgccagc ggatcccata gtgactggat atgttgtgtt ttacagtatt atgtagtctg
    12481 ttttttatgc aaaatctaat ttaatatatt gatatttata tcattttacg tttctcgttc
    12541 agcttttttg tacaaacttg tgctagcatt ctagttgata tctataacaa gaaaatatat
    12601 atataataag ttatcacgta agtagaacat gaaataacaa tataattatc gtatgagtta
    12661 aatcttaaaa gtcacgtaaa agataatcat gcgtcatttt gactcacgcg gtcgttatag
    12721 ttcaaaatca gtgacactta ccgcattgac aagcacgcct cacgggagct ccaagcggcg
    12781 actgagatgt cctaaatgca cagcgacgga ttcgcgctat ttagaaagag agagcaatat
    12841 ttcaagaatg catgcgtcaa ttttacgcag actatctttc tagggttaat ctagaatggc
    12901 gcgccccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca
    12961 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
    13021 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
    13081 cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
    13141 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
    13201 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
    13261 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
    13321 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
    13381 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
    13441 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
    13501 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt
    13561 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
    13621 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
    13681 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
    13741 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
    13801 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
    13861 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
    13921 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
    13981 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
    14041 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
    14101 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
    14161 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
    14221 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
    14281 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
    14341 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
    14401 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
    14461 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
    14521 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
    14581 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
    14641 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
    14701 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
    14761 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
    14821 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
    14881 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
    14941 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
    15001 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
    15061 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
    15121 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
    15181 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
    15241 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
    15301 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
    15361 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
    15421 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
    15481 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
    15541 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aat
//
