LOCUS       sequence-198285-        9966 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PiggyBac transposon system construct with 10 concatenated U6 
            promoter driven transcriptional cassettes targeting OCT4, MYC, KLF4,
            SOX2 and LIN28A promoters. Contains PGK-puro selection cassette..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9966)
  AUTHORS   Weltner J, Balboa D, Katayama S, Bespalov M, Krjutskov K, Jouhilahti
            EM, Trokovic R, Kere J, Otonkoski T
  TITLE     Human pluripotent reprogramming with CRISPR activators.
  JOURNAL   Nat Commun. 2018 Jul 6;9(1):2643. doi: 10.1038/s41467-018-05067-x.
  PUBMED    29980666
REFERENCE   2  (bases 1 to 9966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-07-6"; volume: "9(1):2643. doi"; pages: " 
            10.1038/s41467-018-05067-x"
COMMENT     Sequence Label: PB-GG-OMKSL-PGK-Puro
FEATURES             Location/Qualifiers
     source          1..9966
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..22
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(37..42,43..60,61..67)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    75..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     99..115
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     134..154
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        136..154
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     repeat_region   205..267
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     480..496
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        497..745
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        766..841
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     873..889
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        890..1138
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        1160..1235
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     complement(1247..1263)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1267..1283
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1284..1532
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        1554..1629
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     1661..1677
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1678..1926
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        1948..2023
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     2055..2071
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2072..2320
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        2342..2417
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    2575..2608
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     2951..2967
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2968..3216
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        3238..3313
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     3345..3361
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3362..3610
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        3632..3707
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     complement(3719..3735)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3739..3755
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3756..4004
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        4026..4101
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     4133..4149
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4150..4398
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        4420..4495
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     4527..4543
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4544..4792
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        4814..4889
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    5047..5080
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        5141..5640
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     5573..5592
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5680..6279
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APHNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5680..5699)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     complement(6360..6377)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6366..6590
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6637..6653)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6661..6677
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6685..6691,6692..6709,6710..6715))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6730..6751
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     protein_bind    complement(6810..6934)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     repeat_region   7224..7258
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(7287..7306)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7288..7306)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7316..7332)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7325..7347)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7474..7929
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7561..7580)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7771..7792
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7955..8059
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8060..8128,8129..8920)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8278..8297)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9091..9679
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9580..9599
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
       61 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
      121 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggatt aattaagcgg
      181 ccgcatacgc gtatactaga ttaaccctag aaagataatc atattgtgac gtacgttaaa
      241 gataatcatg cgtaaaattg acgcatgtgt tttatcggtc tgtatatcga ggtttattta
      301 ttaatttgaa tagatattaa gttttattat atttacactt acatactaat aataaattca
      361 acaaacaatt tatttatgtt tatttattta ttaaaaaaaa acaaaaactc aaaatttctt
      421 ctataaagta acaaaacttt taactagagg atctcgagct caagcttcga attccccctg
      481 taaaacgacg gccagtgagg gcctatttcc catgattcct tcatatttgc atatacgata
      541 caaggctgtt agagagataa ttggaattaa tttgactgta aacacaaaga tattagtaca
      601 aaatacgtga cgtagaaagt aataatttct tgggtagttt gcagttttaa aattatgttt
      661 taaaatggac tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct tggctttata
      721 tatcttgtgg aaaggacgaa acaccggggg gagaaactga ggcgagtttt agagctagaa
      781 atagcaagtt aaaataaggc tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg
      841 ctttttttca tggtcatagc tgtttcctca tggtaaaacg acggccagtg agggcctatt
      901 tcccatgatt ccttcatatt tgcatatacg atacaaggct gttagagaga taattggaat
      961 taatttgact gtaaacacaa agatattagt acaaaatacg tgacgtagaa agtaataatt
     1021 tcttgggtag tttgcagttt taaaattatg ttttaaaatg gactatcata tgcttaccgt
     1081 aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac gaaacaccgc
     1141 cctttataat gcgagggtcg ttttagagct agaaatagca agttaaaata aggctagtcc
     1201 gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt ttcatggtca tagctgtttc
     1261 ctggacgtaa aacgacggcc agtgagggcc tatttcccat gattccttca tatttgcata
     1321 tacgatacaa ggctgttaga gagataattg gaattaattt gactgtaaac acaaagatat
     1381 tagtacaaaa tacgtgacgt agaaagtaat aatttcttgg gtagtttgca gttttaaaat
     1441 tatgttttaa aatggactat catatgctta ccgtaacttg aaagtatttc gatttcttgg
     1501 ctttatatat cttgtggaaa ggacgaaaca ccggctgcca tagcaacgat ggagttttag
     1561 agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa agtggcaccg
     1621 agtcggtgct ttttttcatg gtcatagctg tttcctccag gtaaaacgac ggccagtgag
     1681 ggcctatttc ccatgattcc ttcatatttg catatacgat acaaggctgt tagagagata
     1741 attggaatta atttgactgt aaacacaaag atattagtac aaaatacgtg acgtagaaag
     1801 taataatttc ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga ctatcatatg
     1861 cttaccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga
     1921 aacaccgggc acagtgccag aggtctggtt ttagagctag aaatagcaag ttaaaataag
     1981 gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcttttttt catggtcata
     2041 gctgtttcct tgttgtaaaa cgacggccag tgagggccta tttcccatga ttccttcata
     2101 tttgcatata cgatacaagg ctgttagaga gataattgga attaatttga ctgtaaacac
     2161 aaagatatta gtacaaaata cgtgacgtag aaagtaataa tttcttgggt agtttgcagt
     2221 tttaaaatta tgttttaaaa tggactatca tatgcttacc gtaacttgaa agtatttcga
     2281 tttcttggct ttatatatct tgtggaaagg acgaaacacc gtctgtgggg gacctgcact
     2341 ggttttagag ctagaaatag caagttaaaa taaggctagt ccgttatcaa cttgaaaaag
     2401 tggcaccgag tcggtgcttt ttttcatggt catagctgtt tcctaacgag cggccgcagg
     2461 aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg
     2521 ggcgaccaaa ggtcgcccga cgcccaattc cgatcatatt caataaccct taatataact
     2581 tcgtataatg tatgctatac gaagttatta ggtctgaaga ggagtttacg tccagccaag
     2641 cttaggatct cgacctcgaa attctaccgg gtaggggagg cgcttttccc aaggcagtct
     2701 ggagcatgcg ctttagcagc cccgctgggc acttggcgct acacaagtgg cctctggcct
     2761 cgcacacatt ccacatccac cggtaggcgc caaccggctc cgttctttgg tggccccttc
     2821 gcgccacctt ctactcctcc cctagtcagg aagttccccc ccgccccgca gctcgcgtcg
     2881 tgcaggacgt gacaaatgga agtagcacgt ctcactagag gatctcgagc tcaagcttcg
     2941 aattccccct gtaaaacgac ggccagtgag ggcctatttc ccatgattcc ttcatatttg
     3001 catatacgat acaaggctgt tagagagata attggaatta atttgactgt aaacacaaag
     3061 atattagtac aaaatacgtg acgtagaaag taataatttc ttgggtagtt tgcagtttta
     3121 aaattatgtt ttaaaatgga ctatcatatg cttaccgtaa cttgaaagta tttcgatttc
     3181 ttggctttat atatcttgtg gaaaggacga aacaccggtg tcagagaccg gagttgtgtt
     3241 ttagagctag aaatagcaag ttaaaataag gctagtccgt tatcaacttg aaaaagtggc
     3301 accgagtcgg tgcttttttt catggtcata gctgtttcct catggtaaaa cgacggccag
     3361 tgagggccta tttcccatga ttccttcata tttgcatata cgatacaagg ctgttagaga
     3421 gataattgga attaatttga ctgtaaacac aaagatatta gtacaaaata cgtgacgtag
     3481 aaagtaataa tttcttgggt agtttgcagt tttaaaatta tgttttaaaa tggactatca
     3541 tatgcttacc gtaacttgaa agtatttcga tttcttggct ttatatatct tgtggaaagg
     3601 acgaaacacc ggtggctggc aggctggctc tgttttagag ctagaaatag caagttaaaa
     3661 taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt ttttcatggt
     3721 catagctgtt tcctggacgt aaaacgacgg ccagtgaggg cctatttccc atgattcctt
     3781 catatttgca tatacgatac aaggctgtta gagagataat tggaattaat ttgactgtaa
     3841 acacaaagat attagtacaa aatacgtgac gtagaaagta ataatttctt gggtagtttg
     3901 cagttttaaa attatgtttt aaaatggact atcatatgct taccgtaact tgaaagtatt
     3961 tcgatttctt ggctttatat atcttgtgga aaggacgaaa caccgcgggg tactcaagtc
     4021 ttctagtttt agagctagaa atagcaagtt aaaataaggc tagtccgtta tcaacttgaa
     4081 aaagtggcac cgagtcggtg ctttttttca tggtcatagc tgtttcctcc aggtaaaacg
     4141 acggccagtg agggcctatt tcccatgatt ccttcatatt tgcatatacg atacaaggct
     4201 gttagagaga taattggaat taatttgact gtaaacacaa agatattagt acaaaatacg
     4261 tgacgtagaa agtaataatt tcttgggtag tttgcagttt taaaattatg ttttaaaatg
     4321 gactatcata tgcttaccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg
     4381 tggaaaggac gaaacaccgt aattatctgc ccggggggtg ttttagagct agaaatagca
     4441 agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt
     4501 ttcatggtca tagctgtttc cttgttgtaa aacgacggcc agtgagggcc tatttcccat
     4561 gattccttca tatttgcata tacgatacaa ggctgttaga gagataattg gaattaattt
     4621 gactgtaaac acaaagatat tagtacaaaa tacgtgacgt agaaagtaat aatttcttgg
     4681 gtagtttgca gttttaaaat tatgttttaa aatggactat catatgctta ccgtaacttg
     4741 aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgaaaca ccgcaaaacc
     4801 cggcagcgag gctgttttag agctagaaat agcaagttaa aataaggcta gtccgttatc
     4861 aacttgaaaa agtggcaccg agtcggtgct ttttttcatg gtcatagctg tttcctaacg
     4921 agcggccgca ggaaccccta gtgatggagt tggccactcc ctctctgcgc gctcgctcgc
     4981 tcactgaggc cgggcgacca aaggtcgccc gacgcccaat tccgatcata ttcaataacc
     5041 cttaatataa cttcgtataa tgtatgctat acgaagttat taggtctgaa gaggagttta
     5101 cgtccagcca agcttaggat ctcgacctcg aaattctacc gggtagggga ggcgcttttc
     5161 ccaaggcagt ctggagcatg cgctttagca gccccgctgg gcacttggcg ctacacaagt
     5221 ggcctctggc ctcgcacaca ttccacatcc accggtaggc gccaaccggc tccgttcttt
     5281 ggtggcccct tcgcgccacc ttctactcct cccctagtca ggaagttccc ccccgccccg
     5341 cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac gtctcactag tctcgtgcag
     5401 atggacagca ccgctgagca atggaagcgg gtaggccttt ggggcagcgg ccaatagcag
     5461 ctttgctcct tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc
     5521 aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc
     5581 acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc gggcctttcg
     5641 acctgcatcc atctagatct cgagcagctg aagcttacca tgaccgagta caagcccacg
     5701 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     5761 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     5821 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     5881 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     5941 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     6001 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     6061 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     6121 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccacaac
     6181 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     6241 ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac gcccgcccca cgacccgcag
     6301 cgcccgaccg aaaggagcgc acgaccccat gcatcgatga tctagagctc gctgatcagc
     6361 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
     6421 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
     6481 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
     6541 ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc
     6601 ggaaagaacc tgcagcccaa gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     6661 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     6721 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     6781 gtcgggaaac ctgtcgtgcc agcggatccc atagtgactg gatatgttgt gttttacagt
     6841 attatgtagt ctgtttttta tgcaaaatct aatttaatat attgatattt atatcatttt
     6901 acgtttctcg ttcagctttt ttgtacaaac ttgtgctagc attctagttg atatctataa
     6961 caagaaaata tatatataat aagttatcac gtaagtagaa catgaaataa caatataatt
     7021 atcgtatgag ttaaatctta aaagtcacgt aaaagataat catgcgtcat tttgactcac
     7081 gcggtcgtta tagttcaaaa tcagtgacac ttaccgcatt gacaagcacg cctcacggga
     7141 gctccaagcg gcgactgaga tgtcctaaat gcacagcgac ggattcgcgc tatttagaaa
     7201 gagagagcaa tatttcaaga atgcatgcgt caattttacg cagactatct ttctagggtt
     7261 aatctagaat ggcgcgcccc aattcgccct atagtgagtc gtattacgcg cgctcactgg
     7321 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
     7381 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
     7441 cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg
     7501 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     7561 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     7621 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     7681 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     7741 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     7801 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     7861 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc
     7921 ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     7981 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     8041 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     8101 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     8161 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     8221 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     8281 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     8341 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     8401 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     8461 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     8521 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     8581 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     8641 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     8701 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     8761 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     8821 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     8881 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     8941 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     9001 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     9061 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     9121 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     9181 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     9241 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     9301 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     9361 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     9421 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     9481 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     9541 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     9601 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     9661 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     9721 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     9781 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     9841 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     9901 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     9961 cgcaat
//
