LOCUS       sequence-198286-       11538 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Adenovirus for the expression of gRNAs targeting intron 13 of murine
            Bcan and intron 10 of murine Ntrk1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11538)
  AUTHORS   Cook PJ, Thomas R, Kannan R, de Leon ES, Drilon A, Rosenblum MK, 
            Scaltriti M, Benezra R, Ventura A
  TITLE     Somatic chromosomal engineering identifies BCAN-NTRK1 as a potent 
            glioma driver and therapeutic target.
  JOURNAL   Nat Commun. 2017 Jul 11;8:15987. doi: 10.1038/ncomms15987.
  PUBMED    28695888
REFERENCE   2  (bases 1 to 11538)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-07-11"; volume: "8:15987. doi"; pages: " 
            10.1038/ncomms15987"
COMMENT     Sequence Label: Adeno BN
FEATURES             Location/Qualifiers
     source          1..11538
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      223..232
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             232..297
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             304..324
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             349..4449
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             349..4449
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             4450..4497
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     primer_bind     complement(4520..4536)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4520..4536)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     polyA_signal    4556..4690
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4580..4599)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4634..4653
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(4833..4855)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      complement(7930..8518)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8010..8029)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8689..9480,9481..9549))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9312..9331
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9550..9654)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9722..9740
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     repeat_region   9842..9944
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     misc_signal     10032..10182
                     /label=Ad5 Psi
                     /note="packaging signal for adenovirus serotype 5"
     promoter        10232..10480
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        10501..10576
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        10667..10915
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        10937..11012
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        11102..11387
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
ORIGIN
        1 ccttcgcccc gtgccccgct ccgccgccgc ctcgcgccgc ccgccccggc tctgactgac
       61 cgcgttactc ccacaggtga gcgggcggga cggcccttct cctccgggct gtaattagct
      121 gagcaagagg taagggttta agggatggtt ggttggtggg gtattaatgt ttaattacct
      181 ggagcacctg cctgaaatca ctttttttca ggttggaccg gtgccaccat ggactataag
      241 gaccacgacg gagactacaa ggatcatgat attgattaca aagacgatga cgataagatg
      301 gccccaaaga agaagcggaa ggtcggtatc cacggagtcc cagcagccga caagaagtac
      361 agcatcggcc tggacatcgg caccaactct gtgggctggg ccgtgatcac cgacgagtac
      421 aaggtgccca gcaagaaatt caaggtgctg ggcaacaccg accggcacag catcaagaag
      481 aacctgatcg gagccctgct gttcgacagc ggcgaaacag ccgaggccac ccggctgaag
      541 agaaccgcca gaagaagata caccagacgg aagaaccgga tctgctatct gcaagagatc
      601 ttcagcaacg agatggccaa ggtggacgac agcttcttcc acagactgga agagtccttc
      661 ctggtggaag aggataagaa gcacgagcgg caccccatct tcggcaacat cgtggacgag
      721 gtggcctacc acgagaagta ccccaccatc taccacctga gaaagaaact ggtggacagc
      781 accgacaagg ccgacctgcg gctgatctat ctggccctgg cccacatgat caagttccgg
      841 ggccacttcc tgatcgaggg cgacctgaac cccgacaaca gcgacgtgga caagctgttc
      901 atccagctgg tgcagaccta caaccagctg ttcgaggaaa accccatcaa cgccagcggc
      961 gtggacgcca aggccatcct gtctgccaga ctgagcaaga gcagacggct ggaaaatctg
     1021 atcgcccagc tgcccggcga gaagaagaat ggcctgttcg gaaacctgat tgccctgagc
     1081 ctgggcctga cccccaactt caagagcaac ttcgacctgg ccgaggatgc caaactgcag
     1141 ctgagcaagg acacctacga cgacgacctg gacaacctgc tggcccagat cggcgaccag
     1201 tacgccgacc tgtttctggc cgccaagaac ctgtccgacg ccatcctgct gagcgacatc
     1261 ctgagagtga acaccgagat caccaaggcc cccctgagcg cctctatgat caagagatac
     1321 gacgagcacc accaggacct gaccctgctg aaagctctcg tgcggcagca gctgcctgag
     1381 aagtacaaag agattttctt cgaccagagc aagaacggct acgccggcta cattgacggc
     1441 ggagccagcc aggaagagtt ctacaagttc atcaagccca tcctggaaaa gatggacggc
     1501 accgaggaac tgctcgtgaa gctgaacaga gaggacctgc tgcggaagca gcggaccttc
     1561 gacaacggca gcatccccca ccagatccac ctgggagagc tgcacgccat tctgcggcgg
     1621 caggaagatt tttacccatt cctgaaggac aaccgggaaa agatcgagaa gatcctgacc
     1681 ttccgcatcc cctactacgt gggccctctg gccaggggaa acagcagatt cgcctggatg
     1741 accagaaaga gcgaggaaac catcaccccc tggaacttcg aggaagtggt ggacaagggc
     1801 gcttccgccc agagcttcat cgagcggatg accaacttcg ataagaacct gcccaacgag
     1861 aaggtgctgc ccaagcacag cctgctgtac gagtacttca ccgtgtataa cgagctgacc
     1921 aaagtgaaat acgtgaccga gggaatgaga aagcccgcct tcctgagcgg cgagcagaaa
     1981 aaggccatcg tggacctgct gttcaagacc aaccggaaag tgaccgtgaa gcagctgaaa
     2041 gaggactact tcaagaaaat cgagtgcttc gactccgtgg aaatctccgg cgtggaagat
     2101 cggttcaacg cctccctggg cacataccac gatctgctga aaattatcaa ggacaaggac
     2161 ttcctggaca atgaggaaaa cgaggacatt ctggaagata tcgtgctgac cctgacactg
     2221 tttgaggaca gagagatgat cgaggaacgg ctgaaaacct atgcccacct gttcgacgac
     2281 aaagtgatga agcagctgaa gcggcggaga tacaccggct ggggcaggct gagccggaag
     2341 ctgatcaacg gcatccggga caagcagtcc ggcaagacaa tcctggattt cctgaagtcc
     2401 gacggcttcg ccaacagaaa cttcatgcag ctgatccacg acgacagcct gacctttaaa
     2461 gaggacatcc agaaagccca ggtgtccggc cagggcgata gcctgcacga gcacattgcc
     2521 aatctggccg gcagccccgc cattaagaag ggcatcctgc agacagtgaa ggtggtggac
     2581 gagctcgtga aagtgatggg ccggcacaag cccgagaaca tcgtgatcga aatggccaga
     2641 gagaaccaga ccacccagaa gggacagaag aacagccgcg agagaatgaa gcggatcgaa
     2701 gagggcatca aagagctggg cagccagatc ctgaaagaac accccgtgga aaacacccag
     2761 ctgcagaacg agaagctgta cctgtactac ctgcagaatg ggcgggatat gtacgtggac
     2821 caggaactgg acatcaaccg gctgtccgac tacgatgtgg accatatcgt gcctcagagc
     2881 tttctgaagg acgactccat cgacaacaag gtgctgacca gaagcgacaa gaaccggggc
     2941 aagagcgaca acgtgccctc cgaagaggtc gtgaagaaga tgaagaacta ctggcggcag
     3001 ctgctgaacg ccaagctgat tacccagaga aagttcgaca atctgaccaa ggccgagaga
     3061 ggcggcctga gcgaactgga taaggccggc ttcatcaaga gacagctggt ggaaacccgg
     3121 cagatcacaa agcacgtggc acagatcctg gactcccgga tgaacactaa gtacgacgag
     3181 aatgacaagc tgatccggga agtgaaagtg atcaccctga agtccaagct ggtgtccgat
     3241 ttccggaagg atttccagtt ttacaaagtg cgcgagatca acaactacca ccacgcccac
     3301 gacgcctacc tgaacgccgt cgtgggaacc gccctgatca aaaagtaccc taagctggaa
     3361 agcgagttcg tgtacggcga ctacaaggtg tacgacgtgc ggaagatgat cgccaagagc
     3421 gagcaggaaa tcggcaaggc taccgccaag tacttcttct acagcaacat catgaacttt
     3481 ttcaagaccg agattaccct ggccaacggc gagatccgga agcggcctct gatcgagaca
     3541 aacggcgaaa ccggggagat cgtgtgggat aagggccggg attttgccac cgtgcggaaa
     3601 gtgctgagca tgccccaagt gaatatcgtg aaaaagaccg aggtgcagac aggcggcttc
     3661 agcaaagagt ctatcctgcc caagaggaac agcgataagc tgatcgccag aaagaaggac
     3721 tgggacccta agaagtacgg cggcttcgac agccccaccg tggcctattc tgtgctggtg
     3781 gtggccaaag tggaaaaggg caagtccaag aaactgaaga gtgtgaaaga gctgctgggg
     3841 atcaccatca tggaaagaag cagcttcgag aagaatccca tcgactttct ggaagccaag
     3901 ggctacaaag aagtgaaaaa ggacctgatc atcaagctgc ctaagtactc cctgttcgag
     3961 ctggaaaacg gccggaagag aatgctggcc tctgccggcg aactgcagaa gggaaacgaa
     4021 ctggccctgc cctccaaata tgtgaacttc ctgtacctgg ccagccacta tgagaagctg
     4081 aagggctccc ccgaggataa tgagcagaaa cagctgtttg tggaacagca caagcactac
     4141 ctggacgaga tcatcgagca gatcagcgag ttctccaaga gagtgatcct ggccgacgct
     4201 aatctggaca aagtgctgtc cgcctacaac aagcaccggg ataagcccat cagagagcag
     4261 gccgagaata tcatccacct gtttaccctg accaatctgg gagcccctgc cgccttcaag
     4321 tactttgaca ccaccatcga ccggaagagg tacaccagca ccaaagaggt gctggacgcc
     4381 accctgatcc accagagcat caccggcctg tacgagacac ggatcgacct gtctcagctg
     4441 ggaggcgaca aaaggccggc ggccacgaaa aaggccggcc aggcaaaaaa gaaaaagtaa
     4501 gaattcctgc agcccggggg atccactagt tctagagcgg ccgccaccgc ggggagatcc
     4561 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
     4621 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
     4681 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg
     4741 ggaggttttt taaagcaagt aaaacctcta caaatgtggt atggctgatt atgatccggc
     4801 tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
     4861 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
     4921 ggtgttggcg ggtgtcgggg cgcagccatg aggtcgactc tagtccccgc ggtggcagat
     4981 ctggaaggtg ctgaggtacg atgagacccg caccaggtgc agaccctgcg agtgtggcgg
     5041 taaacatatt aggaaccagc ctgtgatgct ggatgtgacc gaggagctga ggcccgatca
     5101 cttggtgctg gcctgcaccc gcgctgagtt tggctctagc gatgaagata cagattgagg
     5161 tactgaaatg tgtgggcgtg gcttaagggt gggaaagaat atataaggtg ggggtcttat
     5221 gtagttttgt atctgttttg cagcagccgc cgccgccatg agcaccaact cgtttgatgg
     5281 aagcattgtg agctcatatt tgacaacgcg catgccccca tgggccgggg tgcgtcagaa
     5341 tgtgatgggc tccagcattg atggtcgccc cgtcctgccc gcaaactcta ctaccttgac
     5401 ctacgagacc gtgtctggaa cgccgttgga gactgcagcc tccgccgccg cttcagccgc
     5461 tgcagccacc gcccgcggga ttgtgactga ctttgctttc ctgagcccgc ttgcaagcag
     5521 tgcagcttcc cgttcatccg cccgcgatga caagttgacg gctcttttgg cacaattgga
     5581 ttctttgacc cgggaactta atgtcgtttc tcagcagctg ttggatctgc gccagcaggt
     5641 ttctgccctg aaggcttcct cccctcccaa tgcggtttaa aacataaata aaaaaccaga
     5701 ctctgtttgg atttggatca agcaagtgtc ttgctgtctt tatttagggg ttttgcgcgc
     5761 gcggtaggcc cgggaccagc ggtctcggtc gttgagggtc ctgtgtattt tttccaggac
     5821 gtggtaaagg tgactctgga tgttcagata catgggcata agcccgtctc tggggtggag
     5881 gtagcaccac tgcagagctt catgctgcgg ggtggtgttg tagatgatcc agtcgtagca
     5941 ggagcgctgg gcgtggtgcc taaaaatgtc tttcagtagc aagctgattg ccaggggcag
     6001 gcccttggtg taagtgttta caaagcggtt aagctgggat gggtgcatac gtggggatat
     6061 gagatgcatc ttggactgta tttttaggtt ggctatgttc ccagccatat ccctccgggg
     6121 attcatgttg tgcagaacca ccagcacagt gtatccggtg cacttgggaa atttgtcatg
     6181 tagcttagaa ggaaatgcgt ggaagaactt ggagacgccc ttgtgacctc caagattttc
     6241 catgcattcg tccataatga tggcaatggg cccacgggcg gcggcctggg cgaagatatt
     6301 tctgggatca ctaacgtcat agttgtgttc caggatgaga tcgtcatagg ccatttttac
     6361 aaagcgcggg cggagggtgc cagactgcgg tataatggtt ccatccggcc caggggcgta
     6421 gttaccctca cagatttgca tttcccacgc tttgagttca gatgggggga tcatgtctac
     6481 ctgcggggcg atgaagaaaa cggtttccgg ggtaggggag atcagctggg aagaaagcag
     6541 gttcctgagc agctgcgact taccgcagcc ggtgggcccg taaatcacac ctattaccgg
     6601 ctgcaactgg tagttaagag agctgcagct gccgtcatcc ctgagcaggg gggccacttc
     6661 gttaagcatg tccctgactc gcatgttttc cctgaccaaa tccgccagaa ggcgctcgcc
     6721 gcccagcgat agcagttctt gcaaggaagc aaagtttttc aacggtttga gaccgtccgc
     6781 cgtaggcatg cttttgagcg tttgaccaag cagttccagg cggtcccaca gctcggtcac
     6841 ctgctctacg gcatctcgat ccagcatatc tcctcgtttc gcgggttggg gcggctttcg
     6901 ctgtacggca gtagtcggtg ctcgtccaga cgggccaggg tcatgtcttt ccacgggcgc
     6961 agggtcctcg tcagcgtagt ctgggtcacg gtgaaggggt gcgctccggg ctgcgcgctg
     7021 gccagggtgc gcttgaggct ggtcctgctg gtgctgaagc gctgccggtc ttcgccctgc
     7081 gcgtcggcca ggtagcattt gaccatggtg tcatagtcca gcccctccgc ggcgtggccc
     7141 ttggcgcgca gcttgccctt ggaggaggcg ccgcacgagg ggcagtgcag acttttgagg
     7201 gcgtagagct tgggcgcgag aaataccgat tccggggagt aggcatccgc gccgcaggcc
     7261 ccgcagacgg tctcgcattc cacgagccag gtgagctctg gccgttcggg gtcaaaaacc
     7321 aggtttcccc catgcttttt gatgcgtttc ttacctctgg tttccatgag ccggtgtcca
     7381 cgctcggtga cgaaaaggct gtccgtgtcc ccgtatacag acttgagagg cctgtcctcg
     7441 accgatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac
     7501 tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc
     7561 agcgctctgg gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct
     7621 gtcgcttgcg gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc
     7681 caccaaacgt ttcggcgaga agcaggccat tatcgccggc atggcggccg acgcgctggg
     7741 ctacgtcttg ctggcgttcg cgacgcgagg ctggatggcc ttccccatta tgattcttct
     7801 cgcttccggc ggcatcggga tgcccgcgtt gcaggccatg ctgtccaggc aggtagatga
     7861 cgaccatcag ggacagcttc aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     7921 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     7981 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     8041 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     8101 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     8161 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     8221 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     8281 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     8341 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
     8401 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     8461 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     8521 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     8581 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     8641 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     8701 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     8761 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     8821 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     8881 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     8941 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     9001 tgctgcaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     9061 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     9121 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     9181 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     9241 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     9301 gtcaacacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     9361 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     9421 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     9481 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     9541 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     9601 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     9661 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
     9721 aaataggcgt atcacgaggc cctttcgtct tcaattaatt aagctagcat catcaataat
     9781 ataccttatt ttggattgaa gccaatatga taatgagggg gtggagaatt aattaagcta
     9841 gcatcatcaa taatatacct tattttggat tgaagccaat atgataatga gggggtggag
     9901 tttgtgacgt ggcgcggggc gtgggaacgg ggcgggtgac gtagtagtgt ggcggaagtg
     9961 tgatgttgca agtgtggcgg aacacatgta agcgacggat gtggcaaaag tgacgttttt
    10021 ggtgtgcgcc ggtgtacaca ggaagtgaca attttcgcgc ggttttaggc ggatgttgta
    10081 gtaaatttgg gcgtaaccga gtaagatttg gccattttcg cgggaaaact gaataagagg
    10141 aagtgaaatc tgaataattt tgtgttactc atagcgcgta atatttgtct agggagatcc
    10201 ggtaccgttt aaactcgaga atggcgcatg tgagggccta tttcccatga ttccttcata
    10261 tttgcatata cgatacaagg ctgttagaga gataattgga attaatttga ctgtaaacac
    10321 aaagatatta gtacaaaata cgtgacgtag aaagtaataa tttcttgggt agtttgcagt
    10381 tttaaaatta tgttttaaaa tggactatca tatgcttacc gtaacttgaa agtatttcga
    10441 tttcttggct ttatatatct tgtggaaagg acgaaacacc gcatgcagtt gaccaagctc
    10501 gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt
    10561 ggcaccgagt cggtgctttt ttgttttaga gctagaaata gcaagttaaa ataaggctag
    10621 tccgttttta gcgcgtgcgc caattctgca gacaaatggc tctagagagg gcctatttcc
    10681 catgattcct tcatatttgc atatacgata caaggctgtt agagagataa ttggaattaa
    10741 tttgactgta aacacaaaga tattagtaca aaatacgtga cgtagaaagt aataatttct
    10801 tgggtagttt gcagttttaa aattatgttt taaaatggac tatcatatgc ttaccgtaac
    10861 ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgaa acaccgtgga
    10921 gctctggaga cctcgtgttt tagagctaga aatagcaagt taaaataagg ctagtccgtt
    10981 atcaacttga aaaagtggca ccgagtcggt gcttttttgt tttagagcta gaaatagcaa
    11041 gttaaaataa ggctagtccg tttttagcgc gtgcgccaat tctgcagaca aatggggtac
    11101 ccgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
    11161 tgacgtcaat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
    11221 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
    11281 acgtcaatga cggtaaatgg cccgcctggc attgtgccca gtacatgacc ttatgggact
    11341 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtc gaggtgagcc
    11401 ccacgttctg cttcactctc cccatctccc ccccctcccc acccccaatt ttgtatttat
    11461 ttatttttta attattttgt gcagcgatgg gggcgggggg gggggggggg cgcgcgccag
    11521 gcggggcggg gcggggcg
//
