LOCUS       sequence-198289-       11710 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Adenovirus for the expression of gRNAs targeting intron 17 of murine
            Fgfr3 and intron 6 of murine Tacc3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11710)
  AUTHORS   Cook PJ, Thomas R, Kannan R, de Leon ES, Drilon A, Rosenblum MK, 
            Scaltriti M, Benezra R, Ventura A
  TITLE     Somatic chromosomal engineering identifies BCAN-NTRK1 as a potent 
            glioma driver and therapeutic target.
  JOURNAL   Nat Commun. 2017 Jul 11;8:15987. doi: 10.1038/ncomms15987.
  PUBMED    28695888
REFERENCE   2  (bases 1 to 11710)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-07-11"; volume: "8:15987. doi"; pages: " 
            10.1038/ncomms15987"
COMMENT     Sequence Label: Adeno FT
FEATURES             Location/Qualifiers
     source          1..11710
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      225..234
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             234..299
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             306..326
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             351..4451
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             351..4451
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             4452..4499
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     primer_bind     complement(4522..4538)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4522..4538)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     polyA_signal    4558..4692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4582..4601)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4636..4655
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(4835..4857)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      complement(7932..8520)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8012..8031)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8691..9482,9483..9551))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9314..9333
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9552..9656)
                     /gene="bla"
                     /label=AmpR promoter
     repeat_region   10011..10113
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     misc_signal     10201..10351
                     /label=Ad5 Psi
                     /note="packaging signal for adenovirus serotype 5"
     promoter        10401..10649
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        10671..10746
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        10837..11085
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        11107..11182
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        11279..11564
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
ORIGIN
        1 tgccttcgcc ccgtgccccg ctccgccgcc gcctcgcgcc gcccgccccg gctctgactg
       61 accgcgttac tcccacaggt gagcgggcgg gacggccctt ctcctccggg ctgtaattag
      121 ctgagcaaga ggtaagggtt taagggatgg ttggttggtg gggtattaat gtttaattac
      181 ctggagcacc tgcctgaaat cacttttttt caggttggac cggtgccacc atggactata
      241 aggaccacga cggagactac aaggatcatg atattgatta caaagacgat gacgataaga
      301 tggccccaaa gaagaagcgg aaggtcggta tccacggagt cccagcagcc gacaagaagt
      361 acagcatcgg cctggacatc ggcaccaact ctgtgggctg ggccgtgatc accgacgagt
      421 acaaggtgcc cagcaagaaa ttcaaggtgc tgggcaacac cgaccggcac agcatcaaga
      481 agaacctgat cggagccctg ctgttcgaca gcggcgaaac agccgaggcc acccggctga
      541 agagaaccgc cagaagaaga tacaccagac ggaagaaccg gatctgctat ctgcaagaga
      601 tcttcagcaa cgagatggcc aaggtggacg acagcttctt ccacagactg gaagagtcct
      661 tcctggtgga agaggataag aagcacgagc ggcaccccat cttcggcaac atcgtggacg
      721 aggtggccta ccacgagaag taccccacca tctaccacct gagaaagaaa ctggtggaca
      781 gcaccgacaa ggccgacctg cggctgatct atctggccct ggcccacatg atcaagttcc
      841 ggggccactt cctgatcgag ggcgacctga accccgacaa cagcgacgtg gacaagctgt
      901 tcatccagct ggtgcagacc tacaaccagc tgttcgagga aaaccccatc aacgccagcg
      961 gcgtggacgc caaggccatc ctgtctgcca gactgagcaa gagcagacgg ctggaaaatc
     1021 tgatcgccca gctgcccggc gagaagaaga atggcctgtt cggaaacctg attgccctga
     1081 gcctgggcct gacccccaac ttcaagagca acttcgacct ggccgaggat gccaaactgc
     1141 agctgagcaa ggacacctac gacgacgacc tggacaacct gctggcccag atcggcgacc
     1201 agtacgccga cctgtttctg gccgccaaga acctgtccga cgccatcctg ctgagcgaca
     1261 tcctgagagt gaacaccgag atcaccaagg cccccctgag cgcctctatg atcaagagat
     1321 acgacgagca ccaccaggac ctgaccctgc tgaaagctct cgtgcggcag cagctgcctg
     1381 agaagtacaa agagattttc ttcgaccaga gcaagaacgg ctacgccggc tacattgacg
     1441 gcggagccag ccaggaagag ttctacaagt tcatcaagcc catcctggaa aagatggacg
     1501 gcaccgagga actgctcgtg aagctgaaca gagaggacct gctgcggaag cagcggacct
     1561 tcgacaacgg cagcatcccc caccagatcc acctgggaga gctgcacgcc attctgcggc
     1621 ggcaggaaga tttttaccca ttcctgaagg acaaccggga aaagatcgag aagatcctga
     1681 ccttccgcat cccctactac gtgggccctc tggccagggg aaacagcaga ttcgcctgga
     1741 tgaccagaaa gagcgaggaa accatcaccc cctggaactt cgaggaagtg gtggacaagg
     1801 gcgcttccgc ccagagcttc atcgagcgga tgaccaactt cgataagaac ctgcccaacg
     1861 agaaggtgct gcccaagcac agcctgctgt acgagtactt caccgtgtat aacgagctga
     1921 ccaaagtgaa atacgtgacc gagggaatga gaaagcccgc cttcctgagc ggcgagcaga
     1981 aaaaggccat cgtggacctg ctgttcaaga ccaaccggaa agtgaccgtg aagcagctga
     2041 aagaggacta cttcaagaaa atcgagtgct tcgactccgt ggaaatctcc ggcgtggaag
     2101 atcggttcaa cgcctccctg ggcacatacc acgatctgct gaaaattatc aaggacaagg
     2161 acttcctgga caatgaggaa aacgaggaca ttctggaaga tatcgtgctg accctgacac
     2221 tgtttgagga cagagagatg atcgaggaac ggctgaaaac ctatgcccac ctgttcgacg
     2281 acaaagtgat gaagcagctg aagcggcgga gatacaccgg ctggggcagg ctgagccgga
     2341 agctgatcaa cggcatccgg gacaagcagt ccggcaagac aatcctggat ttcctgaagt
     2401 ccgacggctt cgccaacaga aacttcatgc agctgatcca cgacgacagc ctgaccttta
     2461 aagaggacat ccagaaagcc caggtgtccg gccagggcga tagcctgcac gagcacattg
     2521 ccaatctggc cggcagcccc gccattaaga agggcatcct gcagacagtg aaggtggtgg
     2581 acgagctcgt gaaagtgatg ggccggcaca agcccgagaa catcgtgatc gaaatggcca
     2641 gagagaacca gaccacccag aagggacaga agaacagccg cgagagaatg aagcggatcg
     2701 aagagggcat caaagagctg ggcagccaga tcctgaaaga acaccccgtg gaaaacaccc
     2761 agctgcagaa cgagaagctg tacctgtact acctgcagaa tgggcgggat atgtacgtgg
     2821 accaggaact ggacatcaac cggctgtccg actacgatgt ggaccatatc gtgcctcaga
     2881 gctttctgaa ggacgactcc atcgacaaca aggtgctgac cagaagcgac aagaaccggg
     2941 gcaagagcga caacgtgccc tccgaagagg tcgtgaagaa gatgaagaac tactggcggc
     3001 agctgctgaa cgccaagctg attacccaga gaaagttcga caatctgacc aaggccgaga
     3061 gaggcggcct gagcgaactg gataaggccg gcttcatcaa gagacagctg gtggaaaccc
     3121 ggcagatcac aaagcacgtg gcacagatcc tggactcccg gatgaacact aagtacgacg
     3181 agaatgacaa gctgatccgg gaagtgaaag tgatcaccct gaagtccaag ctggtgtccg
     3241 atttccggaa ggatttccag ttttacaaag tgcgcgagat caacaactac caccacgccc
     3301 acgacgccta cctgaacgcc gtcgtgggaa ccgccctgat caaaaagtac cctaagctgg
     3361 aaagcgagtt cgtgtacggc gactacaagg tgtacgacgt gcggaagatg atcgccaaga
     3421 gcgagcagga aatcggcaag gctaccgcca agtacttctt ctacagcaac atcatgaact
     3481 ttttcaagac cgagattacc ctggccaacg gcgagatccg gaagcggcct ctgatcgaga
     3541 caaacggcga aaccggggag atcgtgtggg ataagggccg ggattttgcc accgtgcgga
     3601 aagtgctgag catgccccaa gtgaatatcg tgaaaaagac cgaggtgcag acaggcggct
     3661 tcagcaaaga gtctatcctg cccaagagga acagcgataa gctgatcgcc agaaagaagg
     3721 actgggaccc taagaagtac ggcggcttcg acagccccac cgtggcctat tctgtgctgg
     3781 tggtggccaa agtggaaaag ggcaagtcca agaaactgaa gagtgtgaaa gagctgctgg
     3841 ggatcaccat catggaaaga agcagcttcg agaagaatcc catcgacttt ctggaagcca
     3901 agggctacaa agaagtgaaa aaggacctga tcatcaagct gcctaagtac tccctgttcg
     3961 agctggaaaa cggccggaag agaatgctgg cctctgccgg cgaactgcag aagggaaacg
     4021 aactggccct gccctccaaa tatgtgaact tcctgtacct ggccagccac tatgagaagc
     4081 tgaagggctc ccccgaggat aatgagcaga aacagctgtt tgtggaacag cacaagcact
     4141 acctggacga gatcatcgag cagatcagcg agttctccaa gagagtgatc ctggccgacg
     4201 ctaatctgga caaagtgctg tccgcctaca acaagcaccg ggataagccc atcagagagc
     4261 aggccgagaa tatcatccac ctgtttaccc tgaccaatct gggagcccct gccgccttca
     4321 agtactttga caccaccatc gaccggaaga ggtacaccag caccaaagag gtgctggacg
     4381 ccaccctgat ccaccagagc atcaccggcc tgtacgagac acggatcgac ctgtctcagc
     4441 tgggaggcga caaaaggccg gcggccacga aaaaggccgg ccaggcaaaa aagaaaaagt
     4501 aagaattcct gcagcccggg ggatccacta gttctagagc ggccgccacc gcggggagat
     4561 ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa
     4621 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
     4681 aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg
     4741 tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga ttatgatccg
     4801 gctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
     4861 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
     4921 cgggtgttgg cgggtgtcgg ggcgcagcca tgaggtcgac tctagtcccc gcggtggcag
     4981 atctggaagg tgctgaggta cgatgagacc cgcaccaggt gcagaccctg cgagtgtggc
     5041 ggtaaacata ttaggaacca gcctgtgatg ctggatgtga ccgaggagct gaggcccgat
     5101 cacttggtgc tggcctgcac ccgcgctgag tttggctcta gcgatgaaga tacagattga
     5161 ggtactgaaa tgtgtgggcg tggcttaagg gtgggaaaga atatataagg tgggggtctt
     5221 atgtagtttt gtatctgttt tgcagcagcc gccgccgcca tgagcaccaa ctcgtttgat
     5281 ggaagcattg tgagctcata tttgacaacg cgcatgcccc catgggccgg ggtgcgtcag
     5341 aatgtgatgg gctccagcat tgatggtcgc cccgtcctgc ccgcaaactc tactaccttg
     5401 acctacgaga ccgtgtctgg aacgccgttg gagactgcag cctccgccgc cgcttcagcc
     5461 gctgcagcca ccgcccgcgg gattgtgact gactttgctt tcctgagccc gcttgcaagc
     5521 agtgcagctt cccgttcatc cgcccgcgat gacaagttga cggctctttt ggcacaattg
     5581 gattctttga cccgggaact taatgtcgtt tctcagcagc tgttggatct gcgccagcag
     5641 gtttctgccc tgaaggcttc ctcccctccc aatgcggttt aaaacataaa taaaaaacca
     5701 gactctgttt ggatttggat caagcaagtg tcttgctgtc tttatttagg ggttttgcgc
     5761 gcgcggtagg cccgggacca gcggtctcgg tcgttgaggg tcctgtgtat tttttccagg
     5821 acgtggtaaa ggtgactctg gatgttcaga tacatgggca taagcccgtc tctggggtgg
     5881 aggtagcacc actgcagagc ttcatgctgc ggggtggtgt tgtagatgat ccagtcgtag
     5941 caggagcgct gggcgtggtg cctaaaaatg tctttcagta gcaagctgat tgccaggggc
     6001 aggcccttgg tgtaagtgtt tacaaagcgg ttaagctggg atgggtgcat acgtggggat
     6061 atgagatgca tcttggactg tatttttagg ttggctatgt tcccagccat atccctccgg
     6121 ggattcatgt tgtgcagaac caccagcaca gtgtatccgg tgcacttggg aaatttgtca
     6181 tgtagcttag aaggaaatgc gtggaagaac ttggagacgc ccttgtgacc tccaagattt
     6241 tccatgcatt cgtccataat gatggcaatg ggcccacggg cggcggcctg ggcgaagata
     6301 tttctgggat cactaacgtc atagttgtgt tccaggatga gatcgtcata ggccattttt
     6361 acaaagcgcg ggcggagggt gccagactgc ggtataatgg ttccatccgg cccaggggcg
     6421 tagttaccct cacagatttg catttcccac gctttgagtt cagatggggg gatcatgtct
     6481 acctgcgggg cgatgaagaa aacggtttcc ggggtagggg agatcagctg ggaagaaagc
     6541 aggttcctga gcagctgcga cttaccgcag ccggtgggcc cgtaaatcac acctattacc
     6601 ggctgcaact ggtagttaag agagctgcag ctgccgtcat ccctgagcag gggggccact
     6661 tcgttaagca tgtccctgac tcgcatgttt tccctgacca aatccgccag aaggcgctcg
     6721 ccgcccagcg atagcagttc ttgcaaggaa gcaaagtttt tcaacggttt gagaccgtcc
     6781 gccgtaggca tgcttttgag cgtttgacca agcagttcca ggcggtccca cagctcggtc
     6841 acctgctcta cggcatctcg atccagcata tctcctcgtt tcgcgggttg gggcggcttt
     6901 cgctgtacgg cagtagtcgg tgctcgtcca gacgggccag ggtcatgtct ttccacgggc
     6961 gcagggtcct cgtcagcgta gtctgggtca cggtgaaggg gtgcgctccg ggctgcgcgc
     7021 tggccagggt gcgcttgagg ctggtcctgc tggtgctgaa gcgctgccgg tcttcgccct
     7081 gcgcgtcggc caggtagcat ttgaccatgg tgtcatagtc cagcccctcc gcggcgtggc
     7141 ccttggcgcg cagcttgccc ttggaggagg cgccgcacga ggggcagtgc agacttttga
     7201 gggcgtagag cttgggcgcg agaaataccg attccgggga gtaggcatcc gcgccgcagg
     7261 ccccgcagac ggtctcgcat tccacgagcc aggtgagctc tggccgttcg gggtcaaaaa
     7321 ccaggtttcc cccatgcttt ttgatgcgtt tcttacctct ggtttccatg agccggtgtc
     7381 cacgctcggt gacgaaaagg ctgtccgtgt ccccgtatac agacttgaga ggcctgtcct
     7441 cgaccgatgc ccttgagagc cttcaaccca gtcagctcct tccggtgggc gcggggcatg
     7501 actatcgtcg ccgcacttat gactgtcttc tttatcatgc aactcgtagg acaggtgccg
     7561 gcagcgctct gggtcatttt cggcgaggac cgctttcgct ggagcgcgac gatgatcggc
     7621 ctgtcgcttg cggtattcgg aatcttgcac gccctcgctc aagccttcgt cactggtccc
     7681 gccaccaaac gtttcggcga gaagcaggcc attatcgccg gcatggcggc cgacgcgctg
     7741 ggctacgtct tgctggcgtt cgcgacgcga ggctggatgg ccttccccat tatgattctt
     7801 ctcgcttccg gcggcatcgg gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat
     7861 gacgaccatc agggacagct tcaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     7921 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     7981 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     8041 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     8101 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     8161 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     8221 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     8281 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     8341 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
     8401 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     8461 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     8521 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     8581 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     8641 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     8701 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     8761 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     8821 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     8881 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     8941 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     9001 attgctgcag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     9061 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     9121 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     9181 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     9241 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     9301 gcgtcaacac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     9361 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     9421 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     9481 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     9541 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     9601 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     9661 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
     9721 aaaaataggc gtatcacgag gccctttcgt cttcaattaa ttaagctagc atcatcaata
     9781 atatacctta ttttggattg aagccaatat gataatgagg gggtggaggg gttattgtct
     9841 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     9901 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
     9961 taaaaatagg cgtatcacga ggccctttcg tcttcaatta attaagctag catcatcaat
    10021 aatatacctt attttggatt gaagccaata tgataatgag ggggtggagt ttgtgacgtg
    10081 gcgcggggcg tgggaacggg gcgggtgacg tagtagtgtg gcggaagtgt gatgttgcaa
    10141 gtgtggcgga acacatgtaa gcgacggatg tggcaaaagt gacgtttttg gtgtgcgccg
    10201 gtgtacacag gaagtgacaa ttttcgcgcg gttttaggcg gatgttgtag taaatttggg
    10261 cgtaaccgag taagatttgg ccattttcgc gggaaaactg aataagagga agtgaaatct
    10321 gaataatttt gtgttactca tagcgcgtaa tatttgtcta gggagatccg gtaccgttta
    10381 aactcgagaa tggcgcatgt gagggcctat ttcccatgat tccttcatat ttgcatatac
    10441 gatacaaggc tgttagagag ataattggaa ttaatttgac tgtaaacaca aagatattag
    10501 tacaaaatac gtgacgtaga aagtaataat ttcttgggta gtttgcagtt ttaaaattat
    10561 gttttaaaat ggactatcat atgcttaccg taacttgaaa gtatttcgat ttcttggctt
    10621 tatatatctt gtggaaagga cgaaacaccg ggttttatcg gggtatgttg gttttagagc
    10681 tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt
    10741 cggtgctttt ttgttttaga gctagaaata gcaagttaaa ataaggctag tccgttttta
    10801 gcgcgtgcgc caattctgca gacaaatggc tctagagagg gcctatttcc catgattcct
    10861 tcatatttgc atatacgata caaggctgtt agagagataa ttggaattaa tttgactgta
    10921 aacacaaaga tattagtaca aaatacgtga cgtagaaagt aataatttct tgggtagttt
    10981 gcagttttaa aattatgttt taaaatggac tatcatatgc ttaccgtaac ttgaaagtat
    11041 ttcgatttct tggctttata tatcttgtgg aaaggacgaa acaccggttc aggctatgcc
    11101 ccctaagttt tagagctaga aatagcaagt taaaataagg ctagtccgtt atcaacttga
    11161 aaaagtggca ccgagtcggt gcttttttgt tttagagcta gaaatagcaa gttaaaataa
    11221 ggctagtccg tttttagcgc gtgcgccaat tctgcagaca aatggctcta gaggtacccg
    11281 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
    11341 cgtcaatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
    11401 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
    11461 tcaatgacgg taaatggccc gcctggcatt gtgcccagta catgacctta tgggactttc
    11521 ctacttggca gtacatctac gtattagtca tcgctattac catggtcgag gtgagcccca
    11581 cgttctgctt cactctcccc atctcccccc cctccccacc cccaattttg tatttattta
    11641 ttttttaatt attttgtgca gcgatggggg cggggggggg gggggggcgc gcgccaggcg
    11701 gggcggggcg
//
