LOCUS       sequence-198348-        4284 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor Vector containing ZNF79 transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4284)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 4284)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR223-ZNF79
FEATURES             Location/Qualifiers
     source          1..4284
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1595..1694)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1711..1730)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1712..1730)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1735..1751)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1735..1751)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2182..2973
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3066..3654
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3555..3574
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3808..3825
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3984..4011)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4103..4189)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4238..4260
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4252..4269
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4253..4269
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagttggca tgctggagga aggagtactg
      121 ccttccccag gccctgccct tccccaagag gaaaacacag gagaggaagg aatggctgct
      181 ggtctcctca tagctgggcc ccggggatcc acattcttca gcagtgtgac ggtagctttt
      241 gcacaggaag ggtggaggtg cctcgtgtct actccacggg acaggttcaa ggaggggata
      301 ccaggaaagt ccaggagcct tgtcctacta ggacttccag tttcccagcc tggcatgaac
      361 tcccagttgg aacaaaggga aggcgcatgg atgctggagg gcgaagacct gcgaagtccc
      421 tctccaggct ggaagattat atctggatca ccaccagagc aagccctttc tgaagcttca
      481 ttccaagacc catgtgtaga gatgccccct ggggattcag accacgggac cagtgacctt
      541 gagaagagct tcaatctgag accagtcctc tctccgcaac agagagtgcc cgtggaagcg
      601 agacctcgca aatgtgagac acacaccgag agcttcaaga actcggaaat cctgaaacct
      661 cacagagcaa aaccatatgc atgtaatgaa tgtggcaaag ccttcagtta ctgttcttcc
      721 ctttctcagc atcagaagag ccacactgga gagaagccct atgagtgcag tgaatgtggg
      781 aaggccttta gccagagctc atctctcatt cagcaccaga ggattcacac tggagagaag
      841 ccttacaagt gcagtgaatg tggaagagcc ttcagccaga acgccaacct caccaaacac
      901 cagcgaaccc acaccggaga gaagccctac agatgcagcg agtgtgagaa agccttcagt
      961 gactgctcag ctcttgttca gcatcagaga attcataccg gagagaagcc ctacgaatgc
     1021 agcgactgtg ggaaggcctt ccgtcacagt gcaaacctca cgaaccatca gaggactcac
     1081 accggggaga agccctacaa gtgcagcgag tgtgggaagg ccttcagtta ctgcgcagcg
     1141 ttcattcagc accagaggat tcacaccggg gagaagccct acagatgtgc cgcgtgtggg
     1201 aaggccttca gccagagtgc aaacctcaca aaccatcaga ggactcacac tggggagaaa
     1261 ccctacaagt gcagcgagtg tgggaaggcc ttcagccaga gtaccaatct cataatccac
     1321 caaaagaccc acaccgggga gaagccatat aaatgtaatg aatgtgggaa attcttcagt
     1381 gagagctcag ccctcattcg gcatcatata atccacaccg gagaaaaacc ttatgagtgt
     1441 aatgagtgtg gtaaagcgtt taaccagagc tcatccctta gtcagcatca gagaatccac
     1501 acaggcgtga aaccctacga atgcagcgag tgtgggaagg ccttccggtg cagctctgcc
     1561 ttcgttagac atcagagact ccacgccgga gagtayccaa ctttcttgta caaagttggc
     1621 attataagaa agcattgctt atcaatttgt tgcaacgaac aggtcactat cagtcaaaat
     1681 aaaatcatta tttgccatcc agctgatatc ccctatagtg agtcgtatta catggtcata
     1741 gctgtttcct ggcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat
     1801 aaaaatatat catcatgcct cctctagacc agccaggaca gaaatgcctc gacttcgctg
     1861 ctacccaagg ttgccgggtg acgcacaccg tggaaacgga tgaaggcacg aacccagtgg
     1921 acataagcct gttcggttcg taagctgtaa tgcaagtagc gtatgcgctc acgcaactgg
     1981 tccagaacct tgaccgaacg cagcggtggt aacggcgcag tggcggtttt catggcttgt
     2041 tatgactgtt tttttggggt acagtctatg cctcgggcat ccaagcagca agcgcgttac
     2101 gccgtgggtc gatgtttgat gttatggagc agcaacgatg ttacgcagca gggcagtcgc
     2161 cctaaaacaa agttaaacat tatgagggaa gcggtgatcg ccgaagtatc gactcaacta
     2221 tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg
     2281 tacggctccg cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg
     2341 gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact
     2401 tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac
     2461 gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag
     2521 cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc
     2581 ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc
     2641 tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg
     2701 aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt
     2761 tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg
     2821 gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga
     2881 caagaagaag atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg
     2941 aaaggcgaga tcaccaaggt agtcggcaaa taaccctcga gccacccatg accaaaatcc
     3001 cttaacgtga gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     3061 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     3121 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     3181 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     3241 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     3301 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     3361 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     3421 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     3481 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     3541 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     3601 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     3661 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     3721 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     3781 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     3841 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     3901 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatacgcg taccgctagc
     3961 caggaagagt ttgtagaaac gcaaaaaggc catccgtcag gatggccttc tgcttagttt
     4021 gatgcctggc agtttatggc gggcgtcctg cccgccaccc tccgggccgt tgcttcacaa
     4081 cgttcaaatc cgctcccggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac
     4141 agataaaacg aaaggcccag tcttccgact gagcctttcg ttttatttga tgcctggcag
     4201 ttccctactc tcgcgttaac gctagcatgg atgttttccc agtcacgacg ttgtaaaacg
     4261 acggccagtc ttaagctcgg gccc
//
