LOCUS       sequence-198362-        2945 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transcripts the r_oligo part that includes the HIV reverse 
            transcriptase binding site (HTBS).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 892 to 926)
  AUTHORS   Elbaz J, Yin P, Voigt CA
  TITLE     Genetic encoding of DNA nanostructures and their self-assembly in 
            living bacteria.
  JOURNAL   Nat Commun. 2016 Apr 19;7:11179. doi: 10.1038/ncomms11179.
  PUBMED    27091073
REFERENCE   2  (bases 1 to 2945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-04-19"; volume: "7:11179. doi"; pages: " 
            10.1038/ncomms11179"
COMMENT     Sequence Label: pO_72
FEATURES             Location/Qualifiers
     source          1..2945
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..792
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPVESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRTDQLEEFVHYVKGEITKVVGK"
     promoter        892..926
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
     terminator      1152..1183
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     rep_origin      1693..2238
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     2139..2158
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2355..2372
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 atgagggaag cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc
       61 gagcgccatc tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc
      121 ggcctgaagc cacacagtga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa
      181 acaacgcggc gagctttgat caacgacctt ttggaaactt cggcttcccc tgtagagagc
      241 gagattctcc gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt
      301 tatccagcta agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt
      361 atcttcgagc cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa
      421 catagcgttg ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag
      481 gatctatttg aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct
      541 ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc
      601 aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat
      661 cagcccgtca tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc
      721 tcgcgcacag atcagttgga agaatttgtc cactacgtga aaggcgagat caccaaggta
      781 gtcggcaaat aatactagct ccggcaaaaa aacgggcaag gtgtcaccac cctgcccttt
      841 ttctttaaaa ccgaaaagat tacttcgcgt ttgccacctg acgtctaaga attgacggct
      901 agctcagtcc taggtacagt gctagcaaaa ccattcccta aacatttaag atgagacaca
      961 tagcctgcgc acgaatcacg actgcactat aacccgcgaa aaaaaacgtg gcgcccgaac
     1021 agggacggat agctcagtcg gtagagcatc agacttttaa tctgagggtc cagggttcaa
     1081 gtccctgttc gggcgccacg ttttttttcg ctgcaggagt cactaagggt tagttagtta
     1141 gattagcaga aagtcaaaag cctccgaccg gaggcttttg actaaaactt cccttggggt
     1201 tatcattggg gctcactcaa aggcggtaat cagataaaaa aatccttagc tttcgctaag
     1261 gatgatttct gctagagatg gaatagactg gatggatgcg gataaagttg caggaccact
     1321 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     1381 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     1441 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     1501 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     1561 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     1621 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccttaat
     1681 aagatgatct tcttgagatc gttttggtct gcgcgtaatc tcttgctctg aaaacgaaaa
     1741 aactgccttg cagggcggtt tttcgaaggt tctctgagct accaactctt tgaaccgagg
     1801 taactggctt ggaggagcgc agtcaccaaa acttgtcctt tcagtttagc cttaaccggc
     1861 gcatgacttc aagactaact cctctaaatc aattaccagt ggctgctgcc agtggtgctt
     1921 ttgcatgtct ttccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     1981 actgaacggg gggttcgtgc atacagtcca gcttggagcg aactgcctac ccggaactga
     2041 gtgccaggcg tggaatgaga caaacgcggc cataacagcg gaatgacacc ggtaaaccga
     2101 aaggcaggaa caggagagcg cacgagggag ccgccagggg gaaacgcctg gtatctttat
     2161 agtcctgtcg ggtttcgcca ccactgattt gagcgtcaga tttcgtgatg cttgtcaggg
     2221 gggcggagcc tatggaaaaa cggctttgcc gcgtccctct cacttccctg ttaagtatct
     2281 tcctggcatc ttccaggaaa tctccgcctc gttcgtaagc catttccgct cgccgcagtc
     2341 gaacgaccga gcgtagcgag tcagtgagcg aggaagcgga atatatcctg tatcacatat
     2401 tctgctgacg caccggtgca gccttttttc tcctgccaca tgaagcactt cactgacacc
     2461 ctcatcagtg ccaacatagt aagccagtat acactccgct agcgctgagg tctgcctcgt
     2521 gaagaaggtg ttgctgactc ataccaggcc tgaatcgccc catcatccag ccagaaagtg
     2581 agggagccac ggttgatgag agctttgttg taggtggacc agttggtgat tttgaacttt
     2641 tgctttgtca cggaacggtc tgcgttgtcg ggaagatgcg tgatctgatc cttcaactca
     2701 gcaaaagttc gatttattca acaaagccac gttgtgtctc aaaatctctg atgttacatt
     2761 gcacaagata aaaatatatc atcatgaaca ataaaactgt ctgcttacat aaacagtaat
     2821 acaggggtgt ttactagagg ttgatcgggc acgtaagagg ttccaacttt caccataatg
     2881 aaataagatc actaccgggc gtattttttg agttatcgag attttcagga gctaaggaag
     2941 ctaaa
//
