LOCUS       sequence-198364-       10913 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EBNA episome plasmid for U6 promoter-driven expression of 5 gRNAs 
            targeting EEA-motif. Includes PGK-puro selection cassette..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10913)
  AUTHORS   Weltner J, Balboa D, Katayama S, Bespalov M, Krjutskov K, Jouhilahti
            EM, Trokovic R, Kere J, Otonkoski T
  TITLE     Human pluripotent reprogramming with CRISPR activators.
  JOURNAL   Nat Commun. 2018 Jul 6;9(1):2643. doi: 10.1038/s41467-018-05067-x.
  PUBMED    29980666
REFERENCE   2  (bases 1 to 10913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-07-6"; volume: "9(1):2643. doi"; pages: " 
            10.1038/s41467-018-05067-x"
COMMENT     Sequence Label: GG-EBNA-EEA-5guides-PGK-Puro
FEATURES             Location/Qualifiers
     source          1..10913
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..22
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(37..42,43..60,61..67)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    75..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     80..102
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     99..115
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     134..153
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        134..152
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     219..235
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        236..484
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        500..575
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     607..623
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        624..872
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        888..963
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     complement(975..991)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     995..1011
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1012..1260
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        1276..1351
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     1383..1399
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1400..1648
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        1664..1739
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     1771..1787
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1788..2036
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        2052..2127
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    2285..2318
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        2379..2878
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2414..2437)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2811..2830
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             2918..3517
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APHNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2918..2937)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     CDS             3966..5891
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGXGGXGXGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     protein_bind    6059..6092
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      6279..8068
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     primer_bind     complement(8637..8656)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8756..8778
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8816..8834)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8902..9006
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9007..9075,9076..9867)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9225..9244)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10038..10626
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10527..10546
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
       61 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
      121 ttacgccaag ctctaatacg actcactata gggagacaag cttgcatgcc tgcaggtcga
      181 ctctagagga tctcgagctc aagcttcgaa ttccccctgt aaaacgacgg ccagtgaggg
      241 cctatttccc atgattcctt catatttgca tatacgatac aaggctgtta gagagataat
      301 tggaattaat ttgactgtaa acacaaagat attagtacaa aatacgtgac gtagaaagta
      361 ataatttctt gggtagtttg cagttttaaa attatgtttt aaaatggact atcatatgct
      421 taccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga aaggacgaaa
      481 caccgcccag cactttgggg ttttagagct agaaatagca agttaaaata aggctagtcc
      541 gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt ttcatggtca tagctgtttc
      601 ctcatggtaa aacgacggcc agtgagggcc tatttcccat gattccttca tatttgcata
      661 tacgatacaa ggctgttaga gagataattg gaattaattt gactgtaaac acaaagatat
      721 tagtacaaaa tacgtgacgt agaaagtaat aatttcttgg gtagtttgca gttttaaaat
      781 tatgttttaa aatggactat catatgctta ccgtaacttg aaagtatttc gatttcttgg
      841 ctttatatat cttgtggaaa ggacgaaaca ccgaatccca gcactttgtt ttagagctag
      901 aaatagcaag ttaaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg
      961 tgcttttttt catggtcata gctgtttcct ggacgtaaaa cgacggccag tgagggccta
     1021 tttcccatga ttccttcata tttgcatata cgatacaagg ctgttagaga gataattgga
     1081 attaatttga ctgtaaacac aaagatatta gtacaaaata cgtgacgtag aaagtaataa
     1141 tttcttgggt agtttgcagt tttaaaatta tgttttaaaa tggactatca tatgcttacc
     1201 gtaacttgaa agtatttcga tttcttggct ttatatatct tgtggaaagg acgaaacacc
     1261 ggcctcccaa agtgcgtttt agagctagaa atagcaagtt aaaataaggc tagtccgtta
     1321 tcaacttgaa aaagtggcac cgagtcggtg ctttttttca tggtcatagc tgtttcctcc
     1381 aggtaaaacg acggccagtg agggcctatt tcccatgatt ccttcatatt tgcatatacg
     1441 atacaaggct gttagagaga taattggaat taatttgact gtaaacacaa agatattagt
     1501 acaaaatacg tgacgtagaa agtaataatt tcttgggtag tttgcagttt taaaattatg
     1561 ttttaaaatg gactatcata tgcttaccgt aacttgaaag tatttcgatt tcttggcttt
     1621 atatatcttg tggaaaggac gaaacaccgg ctacttggga ggcgttttag agctagaaat
     1681 agcaagttaa aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct
     1741 ttttttcatg gtcatagctg tttccttgtt gtaaaacgac ggccagtgag ggcctatttc
     1801 ccatgattcc ttcatatttg catatacgat acaaggctgt tagagagata attggaatta
     1861 atttgactgt aaacacaaag atattagtac aaaatacgtg acgtagaaag taataatttc
     1921 ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga ctatcatatg cttaccgtaa
     1981 cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga aacaccggcc
     2041 tcccaagtag cgttttagag ctagaaatag caagttaaaa taaggctagt ccgttatcaa
     2101 cttgaaaaag tggcaccgag tcggtgcttt ttttcatggt catagctgtt tcctaacgag
     2161 cggccgcagg aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc
     2221 actgaggccg ggcgaccaaa ggtcgcccga cgcccaattc cgatcatatt caataaccct
     2281 taatataact tcgtataatg tatgctatac gaagttatta ggtctgaaga ggagtttacg
     2341 tccagccaag cttaggatct cgacctcgaa attctaccgg gtaggggagg cgcttttccc
     2401 aaggcagtct ggagcatgcg ctttagcagc cccgctgggc acttggcgct acacaagtgg
     2461 cctctggcct cgcacacatt ccacatccac cggtaggcgc caaccggctc cgttctttgg
     2521 tggccccttc gcgccacctt ctactcctcc cctagtcagg aagttccccc ccgccccgca
     2581 gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt ctcactagtc tcgtgcagat
     2641 ggacagcacc gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc aatagcagct
     2701 ttgctccttc gctttctggg ctcagaggct gggaaggggt gggtccgggg gcgggctcag
     2761 gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac
     2821 gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac
     2881 ctgcatccat ctagatctcg agcagctgaa gcttaccatg accgagtaca agcccacggt
     2941 gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc
     3001 cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga
     3061 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga
     3121 cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt
     3181 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca
     3241 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
     3301 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
     3361 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccacaacct
     3421 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
     3481 gcgcacctgg tgcatgaccc gcaagcccgg tgcctgacgc ccgccccacg acccgcagcg
     3541 cccgaccgaa aggagcgcac gaccccatgc atcgataagc tgatcctcac aggccgcacc
     3601 cagcttttct tccgttgccc cagtagcatc tctgtctggt gaccttgaag aggaagagga
     3661 ggggtcccga gaatccccat ccctaccgtc cagcaaaaag ggggacgagg aatttgaggc
     3721 ctggcttgag gctcaggacg caaatcttga ggatgttcag cgggagtttt ccgggctgcg
     3781 agtaattggt gatgaggacg aggatggttc ggaggatggg gaattttcag acctggatct
     3841 gtctgacagc gaccatgaag gggatgaggg tgggggggct gttggagggg gcaggagtct
     3901 gcactccctg tattcactga gcgtcgtcta ataaagatgt ctattgatct cttttagtgt
     3961 gaatcatgtc tgacgagggg ccaggtacag gacctggaaa tggcctagga gagaagggag
     4021 acacatctgg accagaaggc tccggcggca gtggacctca aagaagaggg ggtgataacc
     4081 atggacgagg acggggaaga ggacgaggac gaggaggcgg aagaccagga gccccgggcg
     4141 gctcaggatc agggccaaga catagagatg gtgtccggag accccaaaaa cgtccaagtt
     4201 gcattggctg caaagggacc cacggtggaa caggagcagg agcaggagcg ggaggggcag
     4261 gagcaggagg ggcaggagca ggaggagggg caggagcagg aggaggggca ggaggggcag
     4321 gaggggcagg aggggcagga gcaggaggag gggcaggagc aggaggaggg gcaggagggg
     4381 caggaggggc aggagcagga ggaggggcag gagcaggagg aggggcagga ggggcaggag
     4441 caggaggagg ggcaggaggg gcaggagggg caggagcagg aggaggggca ggagcaggag
     4501 gaggggcagg aggggcagga gcaggaggag gggcaggagg ggcaggaggg gcaggagcag
     4561 gaggaggggc aggagcagga ggggcaggag gggcaggagg ggcaggagca ggaggggcag
     4621 gagcaggagg aggggcagga ggggcaggag gggcaggagc aggaggggca ggagcaggag
     4681 gggcaggagc aggaggggca ggagcaggag gggcaggagg ggcaggagca ggaggggcag
     4741 gaggggcagg agcaggaggg gcaggagggg caggagcagg aggaggggca ggaggggcag
     4801 gagcaggagg aggggcagga ggggcaggag caggaggggs aggaggggsa ggagsaggag
     4861 gggcaggagg ggcaggagca ggaggggcag gaggggcagg agcaggagga ggggcaggag
     4921 caggaggggc aggagcagga ggtggaggcc ggggtcgagg aggcagtgga ggccggggtc
     4981 gaggaggtag tggaggccgg ggtcgaggag gtagtggagg ccgccggggt agaggacgtg
     5041 aaagagccag ggggggaagt cgtgaaagag ccagggggag aggtcgtgga cgtggagaaa
     5101 agaggcccag gagtcccagt agtcagtcat catcatccgg gtctccaccg cgcaggcccc
     5161 ctccaggtag aaggccattt ttccaccctg taggggaagc cgattatttt gaataccacc
     5221 aagaaggtgg cccagatggt gagcctgacg tgcccccggg agcgatagag cagggccccg
     5281 cagatgaccc aggagaaggc ccaagcactg gaccccgggg tcagggtgat ggaggcaggc
     5341 gcaaaaaagg agggtggttt ggaaagcatc gtggtcaagg aggttccaac ccgaaatttg
     5401 agaacattgc agaaggttta agagctctcc tggctaggag tcacgtagaa aggactaccg
     5461 acgaaggaac ttgggtcgcc ggtgtgttcg tatatggagg tagtaagacc tccctttaca
     5521 acctaaggcg aggaactgcc cttgctattc cacaatgtcg tcttacacca ttgagtcgtc
     5581 tcccctttgg aatggcccct ggacccggcc cacaacctgg cccgctaagg gagtccattg
     5641 tctgttattt catggtcttt ttacaaactc atatatttgc tgaggttttg aaggatgcga
     5701 ttaaggacct tgttatgaca aagcccgctc ctacctgcaa tatcagggtg actgtgtgca
     5761 gctttgacga tggagtagat ttgcctccct ggtttccacc tatggtggaa ggggctgccg
     5821 cggagggtga tgacggagat gacggagatg aaggaggtga tggagatgag ggtgaggaag
     5881 ggcaggagtg atgtaacttg ttaggagacg ccctcaatcg tattaaaagc cgtgtattcc
     5941 cccgcactaa agaataaatc cccagtagac atcatgcgtg ctgttggtgt atttctggcc
     6001 atctgtcttg tcaccatttt cgtcctccca acatggggca attgccggaa cccttaatat
     6061 aacttcgtat aatgtatgct atacgaagtt attaggtccc tcgaagaggt tcactagcgg
     6121 atctcaattg ggcataccca tgttgtcacg tcactcagct ccgcgctcaa caccttctcg
     6181 cgttggaaaa cattagcgac atttacctgg tgagcaatca gacatgcgac ggctttagcc
     6241 tggcctcctt aaattcacct aagaatggga gcaaccagca ggaaaaggac aagcagcgaa
     6301 aattcacgcc cccttgggag gtggcggcat atgcaaagga tagcactccc actctactac
     6361 tgggtatcat atgctgactg tatatgcatg aggatagcat atgctacccg gatacagatt
     6421 aggatagcat atactaccca gatatagatt aggatagcat atgctaccca gatatagatt
     6481 aggatagcct atgctaccca gatataaatt aggatagcat atactaccca gatatagatt
     6541 aggatagcat atgctaccca gatatagatt aggatagcct atgctaccca gatatagatt
     6601 aggatagcat atgctaccca gatatagatt aggatagcat atgctatcca gatatttggg
     6661 tagtatatgc tacccagata taaattagga tagcatatac taccctaatc tctattagga
     6721 tagcatatgc tacccggata cagattagga tagcatatac tacccagata tagattagga
     6781 tagcatatgc tacccagata tagattagga tagcctatgc tacccagata taaattagga
     6841 tagcatatac tacccagata tagattagga tagcatatgc tacccagata tagattagga
     6901 tagcctatgc tacccagata tagattagga tagcatatgc tatccagata tttgggtagt
     6961 atatgctacc catggcaaca ttagcccacc gtgctctcag cgacctcgtg aatatgagga
     7021 ccaacaaccc tgtgcttggc gctcaggcgc aagtgtgtgt aatttgtcct ccagatcgca
     7081 gcaatcgcgc ccctatcttg gcccgcccac ctacttatgc aggtattccc cggggtgcca
     7141 ttagtggttt tgtgggcaag tggtttgacc gcagtggtta gcggggttac aatcagccaa
     7201 gttattacac ccttatttta cagtccaaaa ccgcagggcg gcgtgtgggg gctgacgcgt
     7261 gcccccactc cacaatttca aaaaaaagag tggccacttg tctttgttta tgggccccat
     7321 tggcgtggag ccccgtttaa ttttcggggg tgttagagac aaccagtgga gtccgctgct
     7381 gtcggcgtcc actctctttc cccttgttac aaatagagtg taacaacatg gttcacctgt
     7441 cttggtccct gcctgggaca catcttaata accccagtat catattgcac taggattatg
     7501 tgttgcccat agccataaat tcgtgtgaga tggacatcca gtctttacgg cttgtcccca
     7561 ccccatggat ttctattgtt aaagatattc agaatgtttc attcctacac tagtatttat
     7621 tgcccaaggg gtttgtgagg gttatattgg tgtcatagca caatgccacc actgaacccc
     7681 ccgtccaaat tttattctgg gggcgtcacc tgaaaccttg ttttcgagca cctcacatac
     7741 accttactgt tcacaactca gcagttattc tattagctaa acgaaggaga atgaagaagc
     7801 aggcgaagat tcaggagagt tcactgcccg ctccttgatc ttcagccact gcccttgtga
     7861 ctaaaatggt tcactaccct cgtggaatcc tgaccccatg taaataaaac cgtgacagct
     7921 catggggtgg gagatatcgc tgttccttag gaccctttta ctaaccctaa ttcgatagca
     7981 tatgcttccc gttgggtaac atatgctatt gaattagggt tagtctggat agtatatact
     8041 actacccggg aagcatatgc tacccgttta gggttaacaa gggggcctta taaacactat
     8101 tgctaatgcc ctcttgaggg tccgcttatc ggtagctaca caggcccctc tgattgacgt
     8161 tggtgtagcc tcccgtagtc ttcctgggcc cctgggaggt acatgtcccc cagcattggt
     8221 gtaagagctt cagccaagag ttacacataa aggcaatgtt gtgttgcagt ccacagactg
     8281 caaagtctgc tccaggatga aagccactca gtgttggcaa atgtgcacat ccatttataa
     8341 ggatgtcaac tacagtcaga gaaccccttt gtgtttggtc cccccccgtg tcacatgtgg
     8401 aacagggccc agttggcaag ttgtaccaac caactgaagg gattacatgc actgccccgc
     8461 gaagaagggg cagagatgtc gtagtcaggt ttagttcgtc cggggcgggg atcgatcctc
     8521 tagtaaggga ttttgccgat ttcggtctat tggttaaaaa atgagctgat ttaacaaaaa
     8581 tttaacgcga attttaacaa aatattaacg tttacaattt tatggtgcac tctcagtaca
     8641 atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg
     8701 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     8761 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc
     8821 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     8881 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     8941 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     9001 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     9061 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     9121 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     9181 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     9241 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     9301 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     9361 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     9421 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     9481 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     9541 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     9601 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     9661 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     9721 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     9781 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     9841 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     9901 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     9961 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
    10021 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
    10081 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
    10141 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
    10201 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
    10261 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
    10321 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
    10381 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
    10441 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
    10501 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
    10561 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
    10621 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
    10681 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
    10741 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
    10801 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
    10861 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aat
//
