LOCUS       sequence-198375-        9767 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  N-terminal Dronpa fragment fused to the C-terminus of full-length 
            HER2; for BiFC..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9767)
  AUTHORS   Hertel F, Mo GC, Duwe S, Dedecker P, Zhang J
  TITLE     RefSOFI for Mapping Nanoscale Organization of Protein-Protein 
            Interactions in Living Cells.
  JOURNAL   Cell Rep. 2016 Jan 12;14(2):390-400. doi: 
            10.1016/j.celrep.2015.12.036. Epub 2015 Dec 31.
  PUBMED    26748717
REFERENCE   2  (bases 1 to 9767)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2015.12.036"; journalName: "Cell Rep"; date: 
            "2016-01-12- 12"; volume: "14"; issue: "2"; pages: "390-400"
COMMENT     Sequence Label: pcDNA3-HER2-DMVF-181-N
FEATURES             Location/Qualifiers
     source          1..9767
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      661..670
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(5086..5104)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5087..5104)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5124..5141)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5130..5354
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5400..5828
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5487..5506)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5697..5718
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5837..5857)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5842..6171
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6022..6157
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6084..6103
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6238..7032
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6292..6311)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6902..6921
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7206..7327
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7243..7262)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7297..7316
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7376..7392)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7376..7392)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7389..7411)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7400..7416
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7424..7430,7431..7448,7449..7454))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7469..7490
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7607..7624)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7778..8366)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7858..7877)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8537..9328,9329..9397))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9160..9179
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9398..9502)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9577..9596)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctt
      661 gccaccatgg agctggcggc cttgtgccgc tgggggctcc tcctcgccct cttgcccccc
      721 ggagccgcga gcacccaagt gtgcaccggc acagacatga agctgcggct ccctgccagt
      781 cccgaggccc acctggacat gctccgccac ctctaccagg gctgccaggt ggtgcaggga
      841 aacctggaac tcacctacct gcccaccaat gccagcctgt ccttcctgca ggatatccag
      901 gaggtgcagg gctacgtgct catcgctcac aaccaagtga ggcaggtccc actgcagagg
      961 ctgcggattg tgcgaggcac ccagctcttt gaggacaact atgccctggc cgtgctagac
     1021 aatggagacc cgctgaacaa taccacccct gtcacagggg cctccccagg aggcctgcgg
     1081 gagctgcagc ttcgaagcct cacagagatc ttgaaaggag gggtcttgat ccagcggaac
     1141 ccccagctct gctaccagga cacgattttg tggaaggaca tcttccacaa gaacaaccag
     1201 ctggctctca cactgataga caccaaccgc tctcgggcct gccacccctg ttctccgatg
     1261 tgtaagggct cccgctgctg gggagagagt tctgaggatt gtcagagcct gacgcgcact
     1321 gtctgtgccg gtggctgtgc ccgctgcaag gggccactgc ccactgactg ctgccatgag
     1381 cagtgtgctg ccggctgcac gggccccaag cactctgact gcctggcctg cctccacttc
     1441 aaccacagtg gcatctgtga gctgcactgc ccagccctgg tcacctacaa cacagacacg
     1501 tttgagtcca tgcccaatcc cgagggccgg tatacattcg gcgccagctg tgtgactgcc
     1561 tgtccctaca actacctttc tacggacgtg ggatcctgca ccctcgtctg ccccctgcac
     1621 aaccaagagg tgacagcaga ggatggaaca cagcggtgtg agaagtgcag caagccctgt
     1681 gcccgagtgt gctatggtct gggcatggag cacttgcgag aggtaagggc agttaccagt
     1741 gccaatatcc aggagtttgc tggctgcaag aagatctttg ggagcctggc atttctgccg
     1801 gagagctttg atggggaccc agcctccaac actgccccgc tccagccaga gcagctccaa
     1861 gtgtttgaga ctctggaaga gatcacaggt tacctataca tctcagcatg gccggacagc
     1921 ctgcctgacc tcagcgtctt ccagaacctg caagtaatcc ggggacgaat tctgcacaat
     1981 ggcgcctact cgctgaccct gcaagggctg ggcatcagct ggctggggct gcgctcactg
     2041 agggaactgg gcagtggact ggccctcatc caccataaca cccacctctg cttcgtgcac
     2101 acggtgccct gggaccagct ctttcggaac ccgcaccaag ctctgctcca cactgccaac
     2161 cggccagagg acgagtgtgt gggcgagggc ctggcctgcc accagctgtg cgcccgaggg
     2221 cactgctggg gtccagggcc cacccagtgt gtcaactgca gccagttcct tcggggccag
     2281 gagtgcgtgg aggaatgccg agtactgcag gggctcccca gggagtatgt gaatgccagg
     2341 cactgtttgc cgtgccaccc tgagtgtcag ccccagaatg gctcagtgac ctgttttgga
     2401 ccggaggctg accagtgtgt ggcctgtgcc cactataagg accctccctt ctgcgtggcc
     2461 cgctgcccca gcggtgtgaa acctgacctc tcctacatgc ccatctggaa gtttccagat
     2521 gaggagggcg catgccagcc ttgccccatc aactgcaccc actcctgtgt ggacctggat
     2581 gacaagggct gccccgccga gcagagagcc agccctctga cgtccatcgt ctctgcggtg
     2641 gttggcatcc tgctggtcgt ggtcttgggg gtggtctttg ggatcctcat caagcgacga
     2701 cagcagaaga tccggaagta cacgatgcgg agactgctgc aggaaacgga gctggtggag
     2761 ccgctgacac ctagcggagc gatgcccaac caggcgcaga tgcggatcct gaaagagacg
     2821 gagctgagga aggtgaaggt gcttggatct ggcgcttttg gcacagtcta caagggcatc
     2881 tggatccctg atggggagaa tgtgaaaatt ccagtggcca tcaaagtgtt gagggaaaac
     2941 acatccccca aagccaacaa agaaatctta gacgaagcat acgtgatggc tggtgtgggc
     3001 tccccatatg tctcccgcct tctgggcatc tgcctgacat ccacggtgca gctggtgaca
     3061 cagcttatgc cctatggctg cctcttagac catgtccggg aaaaccgcgg acgcctgggc
     3121 tcccaggacc tgctgaactg gtgtatgcag attgccaagg ggatgagcta cctggaggat
     3181 gtgcggctcg tacacaggga cttggccgct cggaacgtgc tggtcaagag tcccaaccat
     3241 gtcaaaatta cagacttcgg gctggctcgg ctgctggaca ttgacgagac agagtaccat
     3301 gcagatgggg gcaaggtgcc catcaagtgg atggcgctgg agtccattct ccgccggcgg
     3361 ttcacccacc agagtgatgt gtggagttat ggtgtgactg tgtgggagct gatgactttt
     3421 ggggccaaac cttacgatgg gatcccagcc cgggagatcc ctgacctgct ggaaaagggg
     3481 gagcggctgc cccagccccc catctgcacc attgatgtct acatgatcat ggtcaaatgt
     3541 tggatgattg actctgaatg tcggccaaga ttccgggagt tggtgtctga attctcccgc
     3601 atggccaggg acccccagcg ctttgtggtc atccagaatg aggacttggg cccagccagt
     3661 cccttggaca gcaccttcta ccgctcactg ctggaggacg atgacatggg ggacctggtg
     3721 gatgctgagg agtatctggt accccagcag ggcttcttct gtccagaccc tgccccgggc
     3781 gctgggggca tggtccacca caggcaccgc agctcatcta ccaggagtgg cggtggggac
     3841 ctgacactag ggctggagcc ctctgaagag gaggccccca ggtctccact ggcaccctcc
     3901 gaaggggctg gctccgatgt atttgatggt gacctgggaa tgggggcagc caaggggctg
     3961 caaagcctcc ccacacatga ccccagccct ctacagcggt acagtgagga ccccacagta
     4021 cccctgccct ctgagactga tggctacgtt gcccccctga cctgcagccc ccagcctgaa
     4081 tatgtgaacc agccagatgt tcggccccag cccccttcgc cccgagaggg ccctctgcct
     4141 gctgcccgac ctgctggtgc cactctggaa aggcccaaga ctctctcccc agggaagaat
     4201 ggggtcgtca aagacgtttt tgcctttggg ggtgccgtgg agaaccccga gtacttgaca
     4261 ccccagggag gagctgcccc tcagccccac cctcctcctg ccttcagccc agccttcgac
     4321 aacctctatt actgggacca ggacccacca gagcgggggg ctccacccag caccttcaaa
     4381 gggacaccta cggcagagaa cccagagtac ctgggtctgg acgtgccagt gggaggtagc
     4441 ggaggtacta gtggtaccgg tggaggtgga tctggaggaa tgagtgtgat taaaccagac
     4501 atgaagatca agctgcgtat ggaaggcgct gtaaatggac acccgttcgc gattgaagga
     4561 gttggccttg ggaagccttt cgagggaaaa cagagtatag accttaaagt caaagaaggc
     4621 ggacctctgc ctttcgccta tgacatcttg acaactgcgt tctgttacgg caacagggta
     4681 ttcgccaaat acccagaaaa tatagtagac tatttcaagc agtcgtttcc tgagggctac
     4741 tcttgggaac gaagcatgaa ttacgaagac gggggcattt gtaacgcgac aaacgacata
     4801 accctggatg gtgactgtta tatctatgaa attcgatttg atggtgtgaa ctttcctgcc
     4861 aatggtccag ttatgcagaa gaggactgtg aaatgggagc catccactga gaaattgtat
     4921 gtgcgtgatg gagtgctgaa gggtgatgtt aacatggctc tgtcgcttga aggaggtggc
     4981 cattaccgat gtgacgttaa aactacttat aaagctaaga agtaagaatt ctgcagatat
     5041 ccatcacact ggcggccgct cgagcatgca tctagagggc cctattctat agtgtcacct
     5101 aaatgctaga gctcgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
     5161 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
     5221 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
     5281 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc
     5341 ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca
     5401 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     5461 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     5521 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     5581 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     5641 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     5701 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     5761 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     5821 cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
     5881 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
     5941 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
     6001 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     6061 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag
     6121 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg
     6181 ggagcttgta tatccatttt cggatctgat caagagacag gatgaggatc gtttcgcatg
     6241 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc
     6301 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg
     6361 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag
     6421 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc
     6481 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat
     6541 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
     6601 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc
     6661 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag
     6721 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc
     6781 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc
     6841 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata
     6901 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc
     6961 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac
     7021 gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc
     7081 catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt
     7141 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc
     7201 accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     7261 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     7321 tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat
     7381 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     7441 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     7501 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     7561 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     7621 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     7681 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     7741 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     7801 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     7861 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     7921 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     7981 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     8041 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     8101 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     8161 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     8221 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     8281 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     8341 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     8401 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     8461 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     8521 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     8581 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     8641 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     8701 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     8761 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     8821 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     8881 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     8941 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     9001 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     9061 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     9121 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     9181 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     9241 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     9301 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     9361 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     9421 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     9481 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat
     9541 cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg atgccgcata
     9601 gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa
     9661 aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc tgcttagggt
     9721 taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgtt
//
