LOCUS       sequence-198386-        8015 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains: complete oriC region of M. florum L1 (oriC4 fragment), 
            colE1 rep origin, recoded tetM and ereB resistance genes, origin of 
            transfer of RP4 plasmid..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8015)
  AUTHORS   Matteau D, Pepin ME, Baby V, Gauthier S, Arango Giraldo M, Knight 
            TF, Rodrigue S
  TITLE     Development of oriC-Based Plasmids for Mesoplasma florum.
  JOURNAL   Appl Environ Microbiol. 2017 Mar 17;83(7). pii: e03374-16. doi: 
            10.1128/AEM.03374-16. Print 2017 Apr 1.
  PUBMED    28115382
REFERENCE   2  (bases 1 to 8015)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol."; date: "2017-03-17"; volume: "83(7). pii: 
            e03374-16. doi"; pages: " 10.1128/AEM.03374-16. Print 2017 Apr 1"
COMMENT     Sequence Label: pMflET-o4
FEATURES             Location/Qualifiers
     source          1..8015
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      4904..4931
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     5005..5024
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5005..5023
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     5031..5048
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     rep_origin      complement(5131..5719)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5211..5230)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        5725..5743
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     oriT            5805..5914
                     /note="incP origin of transfer"
ORIGIN
        1 tccctttagt gagggttaat gtcgtgactg ggaaaaccct ggcggaattc ggccagtcta
       61 catgtactct ttttgataaa aaattggaga ttcctttaca aatatgctct tacgtgctat
      121 tatttaagtg actatttaaa aggagttaat aaatatgcgg caaggtattc ttaaataaac
      181 tgtcaatttg atagcgggaa caaataatta gatgtccttt tttaggaggg cttagttttt
      241 tgtacccagt ttaagaatac ctttatcatg tgattctaaa gtatccagag aatatctgta
      301 tgctttgtat acctatggtt atgcataaaa atcccggtga taaaagtatt tatcactggg
      361 atttttatgc ccttttgggt ttttgaatgg aggaaatcta gatgaaaatc ataaatatcg
      421 gtgtattagc tcacgttgat gcaggaaaaa caacattaac tgaatcactt ttatataact
      481 ctggtgcaat tactgaactt ggttcagtag ataaaggtac tactcgtact gataatacat
      541 tattagaacg tcaacgtgga atcacaattc aaacaggtat cacatctttt caatgggaaa
      601 atacaaaagt aaatattata gatacacctg gacacatgga tttccttgca gaagtatacc
      661 gtagtctttc agtattagat ggtgctattt tacttatcag cgctaaagat ggagttcaag
      721 ctcaaactcg tatcttattt cacgcattac gtaaaatggg tattccaaca attttcttta
      781 taaacaaaat tgaccaaaac ggaattgatt taagtacagt ttatcaagat atcaaagaaa
      841 aactttctgc tgaaatcgtt attaaacaaa aagttgaatt atacccaaac gtttgcgtaa
      901 caaattttac tgaatcagaa caatgggata cagttataga aggtaatgat gatttattag
      961 aaaaatacat gtcaggtaaa tcattagaag cattagaatt agaacaagaa gaaagtattc
     1021 gtttccaaaa ctgttcttta ttccctttat accatggaag cgctaaaagt aacataggta
     1081 ttgataactt aattgaagtt attactaaca aattttactc ttcaactcat cgtggtcctt
     1141 ctgaattatg cggtaacgtt ttcaaaattg aatatacaaa aaaacgtcaa cgtttagctt
     1201 atatacgtct ttatagtggt gttttacatt tacgtgatag tgttcgtgtt agtgaaaaag
     1261 aaaagattaa agttacagaa atgtatactt ctattaacgg tgaattatgc aaaattgacc
     1321 gtgcatattc aggtgaaatt gtaattttac aaaacgaatt tcttaaactt aatagtgtac
     1381 ttggtgacac aaaactttta ccacaacgta agaaaattga aaatccacac ccattacttc
     1441 aaacaacagt agaaccaagc aaacctgaac aacgtgaaat gcttttagat gctcttttag
     1501 aaattagtga ctctgaccca cttttacgtt actatgtaga ttctactact catgaaatta
     1561 ttctttcttt ccttggtaaa gttcaaatgg aagttatttc tgcattatta caagaaaaat
     1621 atcatgttga aatcgaatta aaagaaccta ctgtaattta tatggaacgt ccattaaaaa
     1681 atgctgaata tacaattcat attgaagttc caccaaatcc attttgggct tctattggtc
     1741 tttctgtttc tccacttcca cttggtagcg gaatgcaata tgaaagtagc gtaagtttag
     1801 gttatcttaa tcaaagtttc caaaacgcag ttatggaagg tattcgttac ggttgcgaac
     1861 aaggtttata cggttggaat gttacagact gcaaaatctg ttttaagtat ggactttact
     1921 attcacctgt atcaacacct gctgactttc gtatgcttgc accaattgtt ttagaacaag
     1981 ttttaaagaa agctggaact gaacttttag aaccatacct ttcttttaaa atctatgcac
     2041 cacaagaata cttaagtcgt gcttataacg atgcacctaa atactgtgct aatattgttg
     2101 atactcaatt aaagaacaac gaagtaattt taagcggaga aattcctgca cgttgtattc
     2161 aagaatatcg tagtgattta acatttttca ctaatggacg ttctgtttgc ttaactgaat
     2221 taaaaggtta tcatgttact actggtgaac ctgtatgcca accacgtcgt cctaatagtc
     2281 gtattgataa agttcgttat atgttcaaca aaatcacata ataatactag taggaggata
     2341 aatagatgaa accagttaca ttgtatgatg tcgctgaata tgctggagtg agttatcaga
     2401 cagtttcaag agttgttaat caagcatcac acgttagcgc aaaaactaga gaaaaagttg
     2461 aagcagcaat ggctgaatta aactatattc caaatagagt tgcacaacaa ttagcaggaa
     2521 aacaatcttt attaattgga gttgcaactt catctcttgc tctacatgct ccatcactaa
     2581 ttgttgcagc aattaaatct agagcagacc aattaggagc ttctgtagtt gtttcaatgg
     2641 ttgaaagaag tggcgttgaa gcatgtaagg cagcagtgca caatttatta gcacaaaggg
     2701 tatcaggtct aattattaat tatccattag atgaccaaga cgcaatagct gtagaagcag
     2761 cttgtactaa tgttccagct ctttttttag atgtatcaga ccaaacacct attaattcaa
     2821 ttattttttc acatgaggat ggaactaggt taggggttga acatttagta gcattaggtc
     2881 atcaacaaat tgcattactt gctggtccac ttagtagtgt ttcagctcgt ttaagattag
     2941 caggatggca tagatattta acacgtaatc aaatacaacc aattgcagaa agagaaggtg
     3001 attggagtgc aatgagtgga tttcaacaaa ccatgcaaat gttaaatgaa ggaatagttc
     3061 caactgccat gttggttgct aatgaccaaa tggcattagg tgcaatgcgt gcaattactg
     3121 aatcaggtct tagagttgga gctgatattt cagttgttgg atatgacgat actgaagact
     3181 cttcatgtta tattccacct cttacaacta taaagcaaga ttttagatta ttaggacgga
     3241 caagcgttga tagactttta caattatcac aagggcaggc tgttaaaggt aatcaattac
     3301 taccagttag tcttgtgaaa agaaaaacta cacttgctcc aaatactcaa actgcttcac
     3361 ctagagcttt agctgattcc ctaatgcaat tagctagaca agtttcaaga ctagaatcag
     3421 gccaataacc atatcatagg gccctactcg caaaaaaccc cgcttcggcg gggttttttc
     3481 gcggattttg ggatggcggc gcgccgcgaa aatctcctgg ttcataaaaa atttatttgc
     3541 tttcaggaaa ttttttctgt ataatagaat agactggagg aaatctagat gagatttgaa
     3601 gaatgggtta aagataaaca tattccattc aaattaaatc accctgatga caactatgat
     3661 gactttaaac cgcttaggaa aattattgga gatactcgtg ttgtagcatt aggagaaaat
     3721 agccacttca ttaaagaatt cttcttactt agacatactt tacttagatt cttcattgag
     3781 gatttaggat ttacaacttt tgcatttgaa tttggttttg ctgaaggaca aattataaat
     3841 aactggatac atggtcaagg tactgatgac gaaattggtc gtttcttaaa acacttttat
     3901 tatccagaag agttgaaaac aacattctta tggttaagag aatataataa agccgcaaaa
     3961 gagaaaatca cattcttagg gattgatatc cctagaaacg gaggttcata tttaccaaac
     4021 atggaaatag tgcatgactt ctttagaact gctgataaag aagctctaca tataattgat
     4081 gacgctttta atattgctaa gaagattgat tacttttcaa cttcacaagc agctttaaat
     4141 ttacatgaat taacagatag tgagaaatgt agattaacaa gtcaattagc aagagtaaaa
     4201 gttagattag aagctatggc accaatccac attgagaaat atggaattga taaatatgaa
     4261 acaatattac attatgcaaa tggaatgatt tatttagatt ataatattca agcaatgagt
     4321 ggttttatta gtggtggagg tatgcaagga gatatgggag caaaagataa atatatggct
     4381 gattctgttt tatggcatct taagaatcca caatcagaac agaaagttat agttgtagct
     4441 cataatgcac atattcagaa aacacctatt ctatatgatg gatttctaag ttgtttacct
     4501 atgggacaaa gattgaagaa tgctataggt gatgattata tgtctttagg tataacatct
     4561 tattctggac atactgctgc attatatcca gaagttgata ctaaatacgg ttttagagtt
     4621 gataattttc aattgcaaga acctaatgaa ggctcagtag aaaaggcaat tagcggttgt
     4681 ggagttacaa actcttttgt attctttaga aatataccag aagatttgca atcaattcca
     4741 aatatgatta gatttgattc aatttacatg aaagctgaat tagagaaagc gtttgatggt
     4801 atatttcaaa tagagaaatc atcagtttca gaagttgttt acgaataact cgagagcgga
     4861 gatttcgggt atcaatgggt accaccttac ctggactcac actggctcac cttcgggtgg
     4921 gcctttctgc gtttatatac tagagagaga atataaaaag ccagattata aatccggctt
     4981 ttttattatt tgcggccgct gcagtaatac gactcactat agggcatacg atttaggtga
     5041 cactatagta gggataacag ggtaatttag taggtagggc tatatggtcg ttatgcgata
     5101 ctgatgcata aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     5161 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     5221 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     5281 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     5341 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     5401 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     5461 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     5521 cggtgctaca gagttcttga agttgtggcc taactacggc tacactagaa gaacagtatt
     5581 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     5641 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     5701 cagaaaaaaa ggatctcaac gcgcaattaa ccctcactaa agggaacaaa agctggagcg
     5761 ctgcaggaat tcgatatcaa gctagcttat cgataccgtc gacgccggcc agcctcgcag
     5821 agcaggattc ccgttgagca ccgccaggtg cgaataaggg acagtgaaga aggaacaccc
     5881 gctcgcgggt gggcctactt cacctatcct gcccggctga cgccgttgga tacaccaagg
     5941 aaagtctaca cgaacccttt ggcaaaatcc tgtatatcgt gcgaaaaagc atggatatac
     6001 cgaaaaaatc gctataatga ccccgaagca gggttatgca gcggaaaagc gctggtaccc
     6061 agcgatgagg atgaacccac tcagggacct gatgtgacgt gccgtgaaca cgagcgtgtt
     6121 tagtttttga tggttgtcaa gttcttttca ttgtattcaa accccctttt aaagttgata
     6181 attcttgtta attataatat aagtgctgga aaaagtatag ttttttattg ttatttttaa
     6241 gaaaatttat ccattgtgtt tttatatgaa ctttttcaac attttttata accttttgtg
     6301 gaaaagcgga aaaaagatat ataaatacta aactattgct ttttagtgtt attcattatg
     6361 tttatttctt tttaaaatat ttttttatcc acacattgtg gaagactttt attattatgt
     6421 gttttaattt attttatgaa ggggaattaa tatggaaaca aaagcgcttt gagaaaaatt
     6481 aattaataag ttaaagaagg aaaaattaat agaccaagac attattgaag aacatattgt
     6541 tacttcagaa ttaattaaaa tttcaaatac agagttcgta atccttgtta gaagtaatct
     6601 tggtgtaact attttaaatg aatttaaaga agtatttgtg tacgaattta aataagtttt
     6661 aaacagttat gtgtctgttg attttttaac taaagaaatt tttgagaaaa atacaaaaaa
     6721 agagaataaa aaagaaccca taaatactgt tttatctgaa aatgcactta cttttgagaa
     6781 ttttatagtt gggtcaagta ataaacaagc taatttagct gctaaaaatg ttgttgcaaa
     6841 ccctggtatg agttttaatc ctttatttat ttatggagat tcaggactgg gtaaaacaca
     6901 tttattacaa gctattaaaa atcaagcaga attaaatgga aaaaaagttt tatatttaac
     6961 atctgaagaa tttacaaaaa gaattgttaa tgctttaaac aaaggtgatt taagtgaaat
     7021 agaagaatta aaaactgaga taaattctaa tgaattcttt attttagatg atgttcaatt
     7081 tttaagtaaa aaagataaaa caaacgagtt tttctttaac attattaaca attttactga
     7141 aaatggtaaa caattagttt tttctagtga taagactcct gaattgttaa atggttttga
     7201 taaaagaatg ataactagat ttaattcagg tttatcaaca cctattaatg ctttagatat
     7261 tccaactgcc aaattgataa ttgaagctga aattaaaaaa caaggtttaa aacaaaaaat
     7321 taaagaagat gccgttgttt acttagctca aaactttagc gatgatgtta gaaaaattaa
     7381 aggtttagtt aatagattac ttttctttgg tattcaaaac gatttaggtc acataattga
     7441 tttggaagat gttattgact tatttaaaga tacaccttca gctaatttag gattattaaa
     7501 tgttaaaaaa attaaagaag ttgttgctaa aaaatatgat gttactataa aagccatcga
     7561 tggtaaagca agaacaactg ctataaaaaa tgctagacat ctttctatgt attttgcaaa
     7621 aataatttta aaccatacat caactcaaat tggtgcagaa tttggtggaa gagaccatag
     7681 tacagtttta agtgctattt cgcgtattga aaaattaata tataaagaaa aagaattcaa
     7741 aaaaatagtt gagtctttaa aaaacgaaat aataggaaaa tagatgtaaa ttcgcatcta
     7801 ttttttttgt gtggaaaaga aattaaactt gttgaataaa aagtcattta aacctttatt
     7861 taccgttgtt ttatgagtgt tttccacata tcaactgcat tataaatatt acttatataa
     7921 agaataatat aatatataaa agcatgcgta tttcttttgt ttttatttta taaatatgag
     7981 ataatatata ttgtaaataa gaggtgttat atgag
//
