LOCUS       sequence-198389-        9361 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EBNA episome plasmid for U6 promoter-driven expression of a control 
            gRNA targeting TdTomato sequence. Includes PGK-puro selection 
            cassette..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9361)
  AUTHORS   Weltner J, Balboa D, Katayama S, Bespalov M, Krjutskov K, Jouhilahti
            EM, Trokovic R, Kere J, Otonkoski T
  TITLE     Human pluripotent reprogramming with CRISPR activators.
  JOURNAL   Nat Commun. 2018 Jul 6;9(1):2643. doi: 10.1038/s41467-018-05067-x.
  PUBMED    29980666
REFERENCE   2  (bases 1 to 9361)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-07-6"; volume: "9(1):2643. doi"; pages: " 
            10.1038/s41467-018-05067-x"
COMMENT     Sequence Label: GG-EBNA-TdT-guide1-PGK-Puro
FEATURES             Location/Qualifiers
     source          1..9361
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..22
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(37..42,43..60,61..67)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    75..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     80..102
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     99..115
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     134..153
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        134..152
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     218..235
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     219..235
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        236..484
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     236..256
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     407..426
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        500..575
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    733..766
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        827..1326
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(862..885)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     1259..1278
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             1366..1965
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APHNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1366..1385)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     CDS             2414..4339
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     protein_bind    4507..4540
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      4727..6516
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     primer_bind     complement(7085..7104)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7204..7226
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7264..7282)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7350..7454
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7455..7523,7524..8315)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7673..7692)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8486..9074
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8975..8994
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
       61 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
      121 ttacgccaag ctctaatacg actcactata gggagacaag cttgcatgcc tgcaggtcga
      181 ctctagagga tctcgagctc aagcttcgrw ytcaccctgt aaaacgacgg ccagtgaggg
      241 cctatttccc atgattcctt catatttgca tatacgatac aaggctgtta gagagataat
      301 tggaattaat ttgactgtaa acacaaagat attagtacaa aatacgtgac gtagaaagta
      361 ataatttctt gggtagtttg cagttttaaa attatgtttt aaaatggact atcatatgct
      421 taccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga aaggacgaaa
      481 caccggagtt cgagatcgag ttttagagct agaaatagca agttaaaata aggctagtcc
      541 gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt ttcatggtca tagctgtttc
      601 ctcatgtgag acgagatctg tcgacgcggc cgcaggaacc cmctccctct ctgcgcgctc
      661 gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg cccaattccg atcatattca
      721 ataaccctta atataacttc gtataatgta tgctatacga agttattagg tctgaagagg
      781 agtttacgtc cagccaagct taggatctcg acctcgaaat tctaccgggt aggggaggcg
      841 cttttcccaa ggcagtctgg agcatgcgct ttagcagccc cgctgggcac ttggcgctac
      901 acaagtggcc tctggcctcg cacacattcc acatccaccg gtaggcgcca accggctccg
      961 ttctttggtg gccccttcgc gccaccttct actcctcccc tagtcaggaa gttccccccc
     1021 gccccgcagc tcgcgtcgtg caggacgtga caaatggaag tagcacgtct cactagtctc
     1081 gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag gcctttgggg cagcggccaa
     1141 tagcagcttt gctccttcgc tttctgggct cagaggctgg gaaggggtgg gtccgggggc
     1201 gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca
     1261 ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc atctccgggc
     1321 ctttcgacct gcatccatct agatctcgag cagctgaagc ttaccatgac cgagtacaag
     1381 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc
     1441 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg
     1501 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
     1561 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg
     1621 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg
     1681 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg
     1741 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
     1801 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc
     1861 cacaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
     1921 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgacgccc gccccacgac
     1981 ccgcagcgcc cgaccgaaag gagcgcacga ccccatgcat cgataagctg atcctcacag
     2041 gccgcaccca gcttttcttc cgttgcccca gtagcatctc tgtctggtga ccttgaagag
     2101 gaagaggagg ggtcccgaga atccccatcc ctaccgtcca gcaaaaaggg ggacgaggaa
     2161 tttgaggcct ggcttgaggc tcaggacgca aatcttgagg atgttcagcg ggagttttcc
     2221 gggctgcgag taattggtga tgaggacgag gatggttcgg aggatgggga attttcagac
     2281 ctggatctgt ctgacagcga ccatgaaggg gatgagggtg ggggggctgt tggagggggc
     2341 aggagtctgc actccctgta ttcactgagc gtcgtctaat aaagatgtct attgatctct
     2401 tttagtgtga atcatgtctg acgaggggcc aggtacagga cctggaaatg gcctaggaga
     2461 gaagggagac acatctggac cagaaggctc cggcggcagt ggacctcaaa gaagaggggg
     2521 tgataaccat ggacgaggac ggggaagagg acgaggacga ggaggcggaa gaccaggagc
     2581 cccgggcggc tcaggatcag ggccaagaca tagagatggt gtccggagac cccaaaaacg
     2641 tccaagttgc attggctgca aagggaccca cggtggaaca ggagcaggag caggagcggg
     2701 aggggcagga gcaggagggg caggagcagg aggaggggca ggagcaggag gaggggcagg
     2761 aggggcagga ggggcaggag gggcaggagc aggaggaggg gcaggagcag gaggaggggc
     2821 aggaggggca ggaggggcag gagcaggagg aggggcagga gcaggaggag gggcaggagg
     2881 ggcaggagca ggaggagggg caggaggggc aggaggggca ggagcaggag gaggggcagg
     2941 agcaggagga ggggcaggag gggcaggagc aggaggaggg gcaggagggg caggaggggc
     3001 aggagcagga ggaggggcag gagcaggagg ggcaggaggg gcaggagggg caggagcagg
     3061 aggggcagga gcaggaggag gggcaggagg ggcaggaggg gcaggagcag gaggggcagg
     3121 agcaggaggg gcaggagcag gaggggcagg agcaggaggg gcaggagggg caggagcagg
     3181 aggggcagga ggggcaggag caggaggggc aggaggggca ggagcaggag gaggggcagg
     3241 aggggcagga gcaggaggag gggcaggagg ggcaggagca ggaggggcag gaggggcagg
     3301 agcaggaggg gcaggagggg caggagcagg aggggcagga ggggcaggag caggaggagg
     3361 ggcaggagca ggaggggcag gagcaggagg tggaggccgg ggtcgaggag gcagtggagg
     3421 ccggggtcga ggaggtagtg gaggccgggg tcgaggaggt agtggaggcc gccggggtag
     3481 aggacgtgaa agagccaggg ggggaagtcg tgaaagagcc agggggagag gtcgtggacg
     3541 tggagaaaag aggcccagga gtcccagtag tcagtcatca tcatccgggt ctccaccgcg
     3601 caggccccct ccaggtagaa ggccattttt ccaccctgta ggggaagccg attattttga
     3661 ataccaccaa gaaggtggcc cagatggtga gcctgacgtg cccccgggag cgatagagca
     3721 gggccccgca gatgacccag gagaaggccc aagcactgga ccccggggtc agggtgatgg
     3781 aggcaggcgc aaaaaaggag ggtggtttgg aaagcatcgt ggtcaaggag gttccaaccc
     3841 gaaatttgag aacattgcag aaggtttaag agctctcctg gctaggagtc acgtagaaag
     3901 gactaccgac gaaggaactt gggtcgccgg tgtgttcgta tatggaggta gtaagacctc
     3961 cctttacaac ctaaggcgag gaactgccct tgctattcca caatgtcgtc ttacaccatt
     4021 gagtcgtctc ccctttggaa tggcccctgg acccggccca caacctggcc cgctaaggga
     4081 gtccattgtc tgttatttca tggtcttttt acaaactcat atatttgctg aggttttgaa
     4141 ggatgcgatt aaggaccttg ttatgacaaa gcccgctcct acctgcaata tcagggtgac
     4201 tgtgtgcagc tttgacgatg gagtagattt gcctccctgg tttccaccta tggtggaagg
     4261 ggctgccgcg gagggtgatg acggagatga cggagatgaa ggaggtgatg gagatgaggg
     4321 tgaggaaggg caggagtgat gtaacttgtt aggagacgcc ctcaatcgta ttaaaagccg
     4381 tgtattcccc cgcactaaag aataaatccc cagtagacat catgcgtgct gttggtgtat
     4441 ttctggccat ctgtcttgtc accattttcg tcctcccaac atggggcaat tgccggaacc
     4501 cttaatataa cttcgtataa tgtatgctat acgaagttat taggtccctc gaagaggttc
     4561 actagcggat ctcaattggg catacccatg ttgtcacgtc actcagctcc gcgctcaaca
     4621 ccttctcgcg ttggaaaaca ttagcgacat ttacctggtg agcaatcaga catgcgacgg
     4681 ctttagcctg gcctccttaa attcacctaa gaatgggagc aaccagcagg aaaaggacaa
     4741 gcagcgaaaa ttcacgcccc cttgggaggt ggcggcatat gcaaaggata gcactcccac
     4801 tctactactg ggtatcatat gctgactgta tatgcatgag gatagcatat gctacccgga
     4861 tacagattag gatagcatat actacccaga tatagattag gatagcatat gctacccaga
     4921 tatagattag gatagcctat gctacccaga tataaattag gatagcatat actacccaga
     4981 tatagattag gatagcatat gctacccaga tatagattag gatagcctat gctacccaga
     5041 tatagattag gatagcatat gctacccaga tatagattag gatagcatat gctatccaga
     5101 tatttgggta gtatatgcta cccagatata aattaggata gcatatacta ccctaatctc
     5161 tattaggata gcatatgcta cccggataca gattaggata gcatatacta cccagatata
     5221 gattaggata gcatatgcta cccagatata gattaggata gcctatgcta cccagatata
     5281 aattaggata gcatatacta cccagatata gattaggata gcatatgcta cccagatata
     5341 gattaggata gcctatgcta cccagatata gattaggata gcatatgcta tccagatatt
     5401 tgggtagtat atgctaccca tggcaacatt agcccaccgt gctctcagcg acctcgtgaa
     5461 tatgaggacc aacaaccctg tgcttggcgc tcaggcgcaa gtgtgtgtaa tttgtcctcc
     5521 agatcgcagc aatcgcgccc ctatcttggc ccgcccacct acttatgcag gtattccccg
     5581 gggtgccatt agtggttttg tgggcaagtg gtttgaccgc agtggttagc ggggttacaa
     5641 tcagccaagt tattacaccc ttattttaca gtccaaaacc gcagggcggc gtgtgggggc
     5701 tgacgcgtgc ccccactcca caatttcaaa aaaaagagtg gccacttgtc tttgtttatg
     5761 ggccccattg gcgtggagcc ccgtttaatt ttcgggggtg ttagagacaa ccagtggagt
     5821 ccgctgctgt cggcgtccac tctctttccc cttgttacaa atagagtgta acaacatggt
     5881 tcacctgtct tggtccctgc ctgggacaca tcttaataac cccagtatca tattgcacta
     5941 ggattatgtg ttgcccatag ccataaattc gtgtgagatg gacatccagt ctttacggct
     6001 tgtccccacc ccatggattt ctattgttaa agatattcag aatgtttcat tcctacacta
     6061 gtatttattg cccaaggggt ttgtgagggt tatattggtg tcatagcaca atgccaccac
     6121 tgaacccccc gtccaaattt tattctgggg gcgtcacctg aaaccttgtt ttcgagcacc
     6181 tcacatacac cttactgttc acaactcagc agttattcta ttagctaaac gaaggagaat
     6241 gaagaagcag gcgaagattc aggagagttc actgcccgct ccttgatctt cagccactgc
     6301 ccttgtgact aaaatggttc actaccctcg tggaatcctg accccatgta aataaaaccg
     6361 tgacagctca tggggtggga gatatcgctg ttccttagga cccttttact aaccctaatt
     6421 cgatagcata tgcttcccgt tgggtaacat atgctattga attagggtta gtctggatag
     6481 tatatactac tacccgggaa gcatatgcta cccgtttagg gttaacaagg gggccttata
     6541 aacactattg ctaatgccct cttgagggtc cgcttatcgg tagctacaca ggcccctctg
     6601 attgacgttg gtgtagcctc ccgtagtctt cctgggcccc tgggaggtac atgtccccca
     6661 gcattggtgt aagagcttca gccaagagtt acacataaag gcaatgttgt gttgcagtcc
     6721 acagactgca aagtctgctc caggatgaaa gccactcagt gttggcaaat gtgcacatcc
     6781 atttataagg atgtcaacta cagtcagaga acccctttgt gtttggtccc cccccgtgtc
     6841 acatgtggaa cagggcccag ttggcaagtt gtaccaacca actgaaggga ttacatgcac
     6901 tgccccgcga agaaggggca gagatgtcgt agtcaggttt agttcgtccg gggcggggat
     6961 cgatcctcta gtaagggatt ttgccgattt cggtctattg gttaaaaaat gagctgattt
     7021 aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttta tggtgcactc
     7081 tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg
     7141 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
     7201 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa
     7261 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     7321 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     7381 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     7441 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     7501 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     7561 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     7621 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     7681 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     7741 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     7801 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     7861 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     7921 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     7981 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     8041 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     8101 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     8161 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     8221 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     8281 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     8341 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     8401 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     8461 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     8521 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     8581 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     8641 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     8701 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     8761 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     8821 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     8881 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     8941 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     9001 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     9061 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     9121 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     9181 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     9241 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     9301 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     9361 t
//
