LOCUS       sequence-198398-       10537 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of EphA2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10537)
  AUTHORS   Song W, Ma Y, Wang J, Brantley-Sieders D, Chen J
  TITLE     JNK signaling mediates EPHA2-dependent tumor cell proliferation, 
            motility, and cancer stem cell-like properties in non-small cell 
            lung cancer.
  JOURNAL   Cancer Res. 2014 May 1;74(9):2444-54. doi: 
            10.1158/0008-5472.CAN-13-2136. Epub 2014 Mar 7.
  PUBMED    24607842
REFERENCE   2  (bases 1 to 10537)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/0008-5472.CAN-13-2136"; journalName: "Cancer Res"; date: 
            "2014-05-1- 1"; volume: "74"; issue: "9"; pages: "2444-54"
COMMENT     Sequence Label: pCLXSN-EphA2-Flag
FEATURES             Location/Qualifiers
     source          1..10537
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     154..174
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(193..210)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(217..264)
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /label=2xFLAG
                     /translation="DYKDDDDKDYKDDDDK"
     CDS             complement(265..279)
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     CDS             complement(292..339)
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /label=2xFLAG
                     /translation="DYKDDDDKDYKDDDDK"
     CDS             complement(3304..3720)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(3320..3336)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(3352..3374)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    3921..4120
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     LTR             4183..4358
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     promoter        complement(4359..4562)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(4388..4408)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        4563..4942
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(5087..5106)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5266..5284)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5352..5456
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5457..5525,5526..6317)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5675..5694)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6488..7076
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6977..6996
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7230..7247
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      7312..7446
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7904..8038
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7928..7947)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7982..8001
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(8310..8330)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          8460..8525
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     LTR             8870..9463
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     CDS             complement(9523..10317)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9634..9653)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     10244..10263
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(join(10377..10537,1..169))
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10391..10526
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 ggggcgggac tatggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg
       61 gggagcctgg ggactttcca cacctggttg ctgactaatt gagatgcatg ctttgcatac
      121 ttctgcctgc tggggagcct ggggactttc cacaccctaa ctgacacaca ttccacagcc
      181 ggatcaaact caatggtgat ggtgatgatg accggtctta tcgtcatcgt ctttgtaatc
      241 cttgtcatcg tcatctttat agtccttatc gtcgtcatcg tctttgtaat ccttgtcatc
      301 gtcatcttta tagtccttat cgtcgtcatc cttgtaatct ctagagatgg ggatccccac
      361 agtgttcacc tggtccttga gtcccagcag gctgtaggcg atgcgcttct ggtggccggg
      421 cagccgcacc ccaatcctct tgatgtcgtc gttggtcatc tgcaccacct tctcgatggc
      481 agtgtagccg gccgccatga agtgctccgt atactgctgc atcttgatgg actccagcca
      541 ctcggacacc gtgcggaagg gcaccccctc cgagccgctc gtgctgggga gccggataga
      601 cacgcggggg tcaaagtcag ccagggtctt gagggagtca ggggcacgaa tgagcttgtc
      661 caggatgctg acgatgtcag cgaacttggg gcggcgggca cgctcctgct gccagcactg
      721 catcatgagc tggtagatgg cggaggggca gtccatgggt gtggggagcc ggaagccatc
      781 attgatggct ttcatcacct cgtggttgga caactcccag tagggccgct cgccataggt
      841 catcacctcc cacatgacaa tgccaaagct ccacacgtcg ctggcagagg tgaacttccg
      901 gtaggaaatg gcctccgggg cggtccagcg gatggggatc ttgccgccac tggtggtgta
      961 ggtggcctcg gggtcgtcct ccagcacgcg ggacaggcca aagtcagaca ccttgcagac
     1021 caggttgctg ttgacgagga tgttgcgggc agccaggtca cggtgcacat agttcatgtt
     1081 ggccaggtac ttcatgccag ctgcgatgcc ccgcagcatg cccaccagct gcagcacgct
     1141 gaactcgcca tccttctccc gaaggaactt gtccagggcc ccattctcca tgtactcagt
     1201 gatgatcatc atgggcttgt atttggagat gacgccctct aggcggatga tgttgtggtg
     1261 gctgaactgg cccatgatgc cggcctcgcc gaggaagtcc actcgctgct tctctgtgta
     1321 gccggctttc agcgtcttga tggccaccgg cacctccttc ttccccgagg atgtcttcag
     1381 catgcccttg tacacctccc caaactctcc tgctccgatc accttctgcc gagtgacaca
     1441 ggatggatgg atctcggtag tgaacttcaa cacagcctgg ttggggtcct catatgtgtg
     1501 ggggtccacg tatgtcttca ggggcttcag ttgttctgac ttggagaagt aaacgtcctc
     1561 cggggactgg cgggcacgct ggttcttcct cctgcggtgg ataaagaagc caactcctgc
     1621 cagcaccaga agcaggacca caccgacagc cacgccgcca atcaccgcca agttgccaga
     1681 tccctccggg gacagcgtct ggaattcgtg caccttgctg ccggccccct ggccctcctg
     1741 cgtcagtgcc tgcacctgga ccaggtaggt ggtgtctggg gccaggtcgt ccagggtcac
     1801 ggagaaaccc tcggtgcggc gcacattgta gctgttggag tctcccttct tgcggtaagt
     1861 gacctcgtac ttccacactc ggctctgctg cggcgggggg atgctccagg agacgctaag
     1921 cgaggtggtg ctgcggccct ccagcctcac cttggggggc tctgtctggt tgatgctgac
     1981 actggcagta cggaagctgc ggctggttac caggcctgag acgccattgc gggcctccac
     2041 ggtgaaggtg tagttcatgt ggggctccag gtcgctcact gtcacactgg tgcgggtcag
     2101 tccgtgagga ggctccgagt agcgcacact ggcctcacac ggcccgcatt ccccagactc
     2161 gggccagcac tgttcgcagg tgacgctgta gacaatgtcc tcgcggcccc cgctgtcctg
     2221 agggggcgtc cagcgcagct ccaccttggc acccatgccc acggctgtga ggtagtgtgg
     2281 ggcggagggg ggtcgtgtgc aaggcatcga cgctgggtcc tgaggtgccc ggaagaagcc
     2341 ttcctcacac tcgcaggagg tggcaccctc aggggatggc agcgtgtgct cagggcactc
     2401 caagcagggg ctctcagatg cctcaaactt aaaaaatcca ggcgagcagg cctggcaggc
     2461 atcctccacc ttctcgtagc ctgcctggca caggcactgc ccaatgggca ccagccactc
     2521 gccatccact gcacagtgca tacggggctc ttcacccccc ggtggcacca cggcatggtc
     2581 cacacaggtg ccggccacag tggccaggga aggtgcatca gagccggcga tggtctcagg
     2641 gaagtgggcc aggccctgca gcagctcggg gcacttcttg tagtagacac ggacggagag
     2701 cagcgccaca caggcaccga tatcctggaa ggccaggtag aagcctttgc gggtgagcgg
     2761 ccccacggag cgctcctcca cgttcagctt cacgtggcgt gcctcgaagt cgctgctgac
     2821 ggtgatctca tcgggcgcaa tggtgtcaat cttggtgaac aggcgcttct ggaagttggt
     2881 gccgtagtcc aggtccgact cggcatagta gaggttgaaa gtctccttgc aggagctggc
     2941 gccaccaggg aagctgttgc agtcacgtac agtaaacttg agctcaatga agatacgctc
     3001 agcctctcct cggtacaccc agttggtgcg gagccagttg tcctggtcgc cagacatcac
     3061 gttgcacacg gagtacatgt agatcggcat gtcattcatg atgttctgca tcaggtccca
     3121 ccctttgcca tacgggtgtg tgagccagcc gagctcccct ccagctgcag caaagtccag
     3181 cagtaccact tccttgccct gcgccgccgc ggccgcggcc agcgcacagc cccacagcag
     3241 ggcgaagcag gcgcgggctg cctggagctc catctcgagt taacgaattg cggccgcaat
     3301 tccggcgcct agagaaggag tgagggctgg ataaagggag gatcgaggcg gggtcgaacg
     3361 aggaggttca agggggagag acggggcgga tggaggaaga ggaggcggag gcttagggtg
     3421 tacaaagggc ttgacccagg gaggggggtc aaaagccaag gcttcccagg tcacgatgta
     3481 ggggacctgg tctgggtgtc catgcgggcc aggtgaaaag accttgatct taacctgggt
     3541 gatgaggtct cggttaaagg tgccgtctcg cggccatccg acgttaaagg ttggccattc
     3601 tgcagagcag aaggtaaccc aacgtctctt cttgacatct accgactggt tgtgagcgat
     3661 ccgctcgaca tctttccagt gacctaaggt caaacttaag ggagtggtaa cagtctggcc
     3721 ctaattttca gacaaataca gaaacacagt cagacagaga caacacagaa cgatgctgca
     3781 gcgctgcagc agacaagacg cgcggcttcg gttccaaacc gaaagcaaaa attcagacgg
     3841 aggcgggaac tgttttaggt tctcgtctcc taccagaacc acatatcctg acggggtcgg
     3901 attccacatc gactcccttc ctcaggtcgg gccacaaaaa cggcccccaa agtccctggg
     3961 acgtctccca gggttgcggc cgggtgttca gaactcgtca gttccaccac gggtccgcca
     4021 gatacagagc tagttagcta actagtacag acgcaggcgc ataacatcaa acatagacac
     4081 tagacaatcg gacagacaca gataagttgc tggccagctt acctcccggt ggtgggtcgg
     4141 tggtccctgg gcaggggtct ccaaatcccg gacgagcccc caaatgaaag acccccgtcg
     4201 tgggtagtca atcactcaga ggagaccctc ccaaggaaca gcgagaccac gattcggatg
     4261 caaacagcaa gaggctttat tgggaatacg ggtacccggg cgacgcagtc tatcggaaga
     4321 ctggcgcgcc gagtgagggg ttgtgggctc ttttattgag ctctgcttat atagacctcc
     4381 caccgtacac gcctaccgcc catttgcgtc aatggggcgg agttgttacg acattttgga
     4441 aagtcccgtt gattttggtg ccaaaacaaa ctcccattga cgtcaatggg gtggagactt
     4501 ggaaatcccc gtgagtcaaa ccgctatcca cgcccattga tgtactgcca aaaccgcatc
     4561 accatggtaa tagcgatgac taatacgtag atgtactgcc aagtaggaaa gtcccataag
     4621 gtcatgtact gggcataatg ccaggcgggc catttaccgt cattgacgtc aatagggggc
     4681 gtacttggca tatgatacac ttgatgtact gccaagtggg cagtttaccg taaatactcc
     4741 acccattgac gtcaatggaa agtccctatt ggcgttacta tgggaacata cgtcattatt
     4801 gacgtcaatg ggcgggggtc gttgggcggt cagccaggcg ggccatttac cgtaagttat
     4861 gtaacgcgga actccatata tgggctatga actaatgacc ccgtaattga ttactattaa
     4921 taactagtca ataatcaatg tcaacgcgct ggcgtaatag cgaagaggcc cgcaccgatc
     4981 gcccttccca acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg tattttctcc
     5041 ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg
     5101 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
     5161 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
     5221 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc
     5281 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
     5341 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     5401 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     5461 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     5521 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     5581 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     5641 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     5701 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     5761 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     5821 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     5881 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     5941 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     6001 cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     6061 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     6121 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     6181 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     6241 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     6301 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     6361 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     6421 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     6481 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     6541 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     6601 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     6661 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     6721 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     6781 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     6841 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     6901 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     6961 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     7021 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     7081 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     7141 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     7201 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     7261 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agcctccaaa
     7321 aaagcctcct cactacttct ggaatagctc agaggccgag gcggcctcgg cctctgcata
     7381 aataaaaaaa attagtcagc catggggcgg agaatgggcg gaactgggcg gagttagggg
     7441 cgggatgggc ggagtaagac ccgcacccgt tcccaaactg cttattaaag ggggtatgga
     7501 ggtgctggac cttgtgacag ggccagacag tgtgacagaa atagaagcag tcctgttttg
     7561 acaagttgcc tctggaagcc tctacaatgc ctctcttctt tttctccaga gtaagcggag
     7621 gccaggggcc cccggcctct gcttaatact aaaaaaaaca gctgttgtca tagtaatgat
     7681 tgggtggaaa cattctaggc ctgggtggag aggctttttg cttcctcttg caaaaccaca
     7741 ctgccctctg gagggcagtt gcctagcaac taattaaaag aggatgtcgc acggccagct
     7801 gcggtcagtt agtcacttcc tgcttaactg acttgacatt ttctatttta agagtcggga
     7861 ggaaaattac tgtgttggag gccctccgcc atcttctgaa gctgatccag acatgataag
     7921 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg
     7981 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
     8041 caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta
     8101 aagcaagtaa aacctctaca aatgtggtat ggctgattat gatctctagt caaggcacta
     8161 tacatcaaat atttttccat aattttcttg tatagcagtg cagctttttc ctttgtggtg
     8221 taaatagcaa agcaagcaag agttctatta ctaaacacag catgactcaa aaaacttagc
     8281 aattctgaag gaaagtcctt ggggtcttct acctttctct tcttttttgg aggagtagaa
     8341 tgttgagagt cagcagtagc ctcatcatca ctagatggca tttcttctga gcaaaacagg
     8401 ttttcctcat taaaggcatt ccaccactgc tcccattcat cagttccata ggttggaatc
     8461 taaaatacac aaacaattag aatcagtagt ttaacacatt atacacttaa aaattttata
     8521 tttaccttag agctttaaat ctctgtaggt agtttgtcca attatgtcac accacagaag
     8581 taaggttcct tcacaaagat cctctagcta cctagctaga tacactccgc tatcgctacg
     8641 tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
     8701 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
     8761 tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ccagcttacc tcccggtggt
     8821 gggtcggtgg tccctgggca ggggtctccc gatcccggac gagcccccaa atgaaagacc
     8881 cccgctgacg ggtagtcaat cactcagagg agaccctccc aaggaacagc gagaccacaa
     8941 gtcggatgca actgcaagag ggtttattgg atacacgggt acccgggcga ctcagtcaat
     9001 cggaggactg gcgccccgag tgaggggttg tgggctcttt tattgagctc ggggagcaga
     9061 agcgcgcgaa cagaagcgag aagcgaactg attggttagt tcaaataagg cacagggtca
     9121 tttcaggtcc ttggggcacc ctggaaacat ctgatggttc tctagaaact gctgagggct
     9181 ggaccgcatc tggggaccat ctgttcttgg ccctgagccg gggcaggaac tgcttaccac
     9241 agatatcctg tttggcccat attcagctgt tccatctgtt cttggccctg agccggggca
     9301 ggaactgctt accacagata tcctgtttgg cccatattca gctgttccat ctgttcctga
     9361 ccttgatctg aacttctcta ttctcagtta tgtatttttc catgccttgc aaaatggcgt
     9421 tacttaagct agcttgccaa acctacaggt ggggtctttc attcccccct ttttctggag
     9481 actaaataaa atcttttatt ttatcgaacc ccagagtccc gctcagaaga actcgtcaag
     9541 aaggcgatag aaggcgatgc gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa
     9601 gcggtcagcc cattcgccgc caagctcttc agcaatatca cgggtagcca acgctatgtc
     9661 ctgatagcgg tccgccacac ccagccggcc acagtcgatg aatccagaaa agcggccatt
     9721 ttccaccatg atattcggca agcaggcatc gccatgggtc acgacgagat cctcgccgtc
     9781 gggcatgcgc gccttgagcc tggcgaacag ttcggctggc gcgagcccct gatgctcttc
     9841 gtccagatca tcctgatcga caagaccggc ttccatccga gtacgtgctc gctcgatgcg
     9901 atgtttcgct tggtggtcga atgggcaggt agccggatca agcgtatgca gccgccgcat
     9961 tgcatcagcc atgatggata ctttctcggc aggagcaagg tgagatgaca ggagatcctg
    10021 ccccggcact tcgcccaata gcagccagtc ccttcccgct tcagtgacaa cgtcgagcac
    10081 agctgcgcaa ggaacgcccg tcgtggccag ccacgatagc cgcgctgcct cgtcctgcag
    10141 ttcattcagg gcaccggaca ggtcggtctt gacaaaaaga accgggcgcc cctgcgctga
    10201 cagccggaac acggcggcat cagagcagcc gattgtctgt tgtgcccagt catagccgaa
    10261 tagcctctcc acccaagcgg ccggagaacc tgcgtgcaat ccatcttgtt caatcatgcg
    10321 aaacgatcct catcctgtct cttgatcaga tccgcctcga cctgcagccc aagctttttg
    10381 caaaagccta ggcctccaaa aaagcctcct cactacttct ggaatagctc agaggccgag
    10441 gcggcctcgg cctctgcata aataaaaaaa attagtcagc catggggcgg agaatgggcg
    10501 gaactgggcg gagttagggg cgggatgggc ggagtta
//
