LOCUS       sequence-198414-        4017 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses ATP sensor in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4017)
  AUTHORS   Lobas MA, Tao R, Nagai J, Kronschlager MT, Borden PM, Marvin JS, 
            Looger LL, Khakh BS
  TITLE     A genetically encoded single-wavelength sensor for imaging cytosolic
            and cell surface ATP.
  JOURNAL   Nat Commun. 2019 Feb 12;10(1):711. doi: 10.1038/s41467-019-08441-5.
  PUBMED    30755613
REFERENCE   2  (bases 1 to 4017)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-02-12"; volume: "10(1):711. doi"; pages: " 
            10.1038/s41467-019-08441-5"
COMMENT     Sequence Label: HHM-iATPSnFR1.1
FEATURES             Location/Qualifiers
     source          1..4017
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             51..73
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             84..101
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             105..131
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     692..713
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(816..837)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             1302..1331
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(1400..1418)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1414..1461
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      1558..2012
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1645..1664)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1855..1876
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2038..2142
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2143..2211,2212..3003)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2361..2380)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3174..3762
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3663..3682
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3916..3933
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac
       61 tttaagaagg agatatacat atgcatcatc atcatcatca tggttatccc tatgatgttc
      121 cagattatgc tggggcccag ccggccagat ctatgaagac tattcacgtg agtgtcgtaa
      181 ctcccgacgg gcctgtatat gaggatgacg ttgaaatggt gagcgtcaaa gcaaaaagtg
      241 gcgagctcgg tattctccca ggccacattc ccttgaaagc tcccctagag atcagtgccg
      301 cacgcctgaa gaaagggggc aaaacacagt atatcgctgt gtcaggcggc aacctcgaag
      361 tgcggcctga caaggtgacc atctatgccc aggcagccga gagggctgag gatatcgatg
      421 tcctgcgcgc caagaaagcc aaggagagag ccgagcgccg actccaatca caggtcctga
      481 gccacaacgt ctatatcacc gccgacaagc agaagaacgg catcaaggcg aacttcaaga
      541 tccgccacaa cgtggaggac ggcagcatgc agctcgccga ccactaccag cagaacaccc
      601 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgtgc
      661 tgagcaaaga ccctaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgtcg
      721 ccgggatcac tctcggcatg gacgagctgt acaagggcgg taccggaggg agcatgagca
      781 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
      841 acggccacaa gttcagcgtg cgcggcgagg gcgagggcga tgccaccaac ggcaagctga
      901 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
      961 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
     1021 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcagc ttcaaggacg
     1081 acggcaccta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     1141 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     1201 acaactttgg gttgcacgac atcgatttta agcgcgccga gctcagcctt aagcgcgcaa
     1261 tgaataggct ctcagttgcc gaaatgaagc tgcaggtcga cgaacaaaaa ctcatctcag
     1321 aagaggatct gaattaagaa ttcgaagctt gatccggctg ctaacaaagc ccgaaaggaa
     1381 gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa
     1441 cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatctgg cgtaatagcg
     1501 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg
     1561 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     1621 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     1681 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     1741 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     1801 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     1861 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     1921 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     1981 cgaattttaa caaaatatta cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc
     2041 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     2101 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     2161 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     2221 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     2281 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     2341 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     2401 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     2461 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     2521 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     2581 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     2641 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     2701 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     2761 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     2821 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     2881 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     2941 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     3001 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     3061 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     3121 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     3181 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     3241 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     3301 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     3361 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     3421 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     3481 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     3541 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     3601 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     3661 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     3721 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     3781 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     3841 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     3901 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     3961 ccgcctctcc ccgcgcgttg gccgattcat taatgcagga tctcgatccc gcgaaat
//
