LOCUS       sequence-198424-        5923 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5923)
  AUTHORS   Rice JJ, Daugherty PS
  TITLE     Directed evolution of a biterminal bacterial display scaffold 
            enhances the display of diverse peptides.
  JOURNAL   Protein Eng Des Sel. 2008 Jul . 21(7):435-42.
  PUBMED    18480093
REFERENCE   2  (bases 1 to 5923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein Eng
            Des Sel. 2008 Jul . 21(7):435-42."
COMMENT     Sequence Label: pB33eCPX
FEATURES             Location/Qualifiers
     source          1..5923
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      338..793
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(425..444)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     635..656
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(1349..2008)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1923..1942
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(2009..2111)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(2503..2520)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2637..3182)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(2717..2736)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3258..3277
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     CDS             complement(3457..4335)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        4362..4646
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     4569..4588
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     primer_bind     complement(5318..5335)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      5488..5574
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      5666..5693
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        5712..5803
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
       61 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
      121 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
      181 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
      241 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
      301 cagaccaagt ttactcatat atactttaga ttgatttacg cgccctgtag cggcgcatta
      361 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
      421 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
      481 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
      541 aaaaaacttg atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt
      601 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
      661 acactcaacc ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggcc
      721 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
      781 acgtttacaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
      841 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
      901 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
      961 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     1021 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
     1081 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtcag
     1141 gcatttgaga agcacacggt cacactgctt ccggtagtca ataaaccggt aaaccagcaa
     1201 tagacataag cggctattta acgaccctgc cctgaaccga cgaccgggtc gaatttgctt
     1261 tcgaatttct gccattcatc cgcttattat cacttattca ggcgtagcaa ccaggcgttt
     1321 aagggcacca ataactgcct taaaaaaatt acgccccgcc ctgccactca tcgcagtact
     1381 gttgtaattc attaagcatt ctgccgacat ggaagccatc acaaacggca tgatgaacct
     1441 gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc atggtgaaaa
     1501 cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg aaactcaccc
     1561 agggattggc tgagacgaaa aacatattct caataaaccc tttagggaaa taggccaggt
     1621 tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt
     1681 ggtattcact ccagagcgat gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag
     1741 ggtgaacact atcccatatc accagctcac cgtctttcat tgccatacgg aattccggat
     1801 gagcattcat caggcgggca agaatgtgaa taaaggccgg ataaaacttg tgcttatttt
     1861 tctttacggt ctttaaaaag gccgtaatat ccagctgaac ggtctggtta taggtacatt
     1921 gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat atatcaacgg
     1981 tggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgaa aatctcgata
     2041 actcaaaaaa tacgcccggt agtgatctta tttcattatg gtgaaagttg gaacctctta
     2101 cgtgccgatc aacgtctcat tttcgccaaa agttggccca gggcttcccg gtatcaacag
     2161 ggacaccagg atttatttat tctgcgaagt gatcttccgt cacaggtatt tattcggcgc
     2221 aaagtgcgtc gggtgatgct gccaacttac tgatttagtg tatgatggtg tttttgaggt
     2281 gctccagtgg cttctgtttc tatcagctgt ccctcctgtt cagctactga cggggtggtg
     2341 cgtaacggca aaagcaccgc cggacatcag cgctagcgga gtgtatactg gcttactatg
     2401 ttggcactga tgagggtgtc agtgaagtgc ttcatgtggc aggagaaaaa aggctgcacc
     2461 ggtgcgtcag cagaatatgt gatacaggat atattccgct tcctcgctca ctgactcgct
     2521 acgctcggtc gttcgactgc ggcgagcgga aatggcttac gaacggggcg gagatttcct
     2581 ggaagatgcc aggaagatac ttaacaggga agtgagaggg ccgcggcaaa gccgtttttc
     2641 cataggctcc gcccccctga caagcatcac gaaatctgac gctcaaatca gtggtggcga
     2701 aacccgacag gactataaag ataccaggcg tttccccctg gcggctccct cgtgcgctct
     2761 cctgttcctg cctttcggtt taccggtgtc attccgctgt tatggccgcg tttgtctcat
     2821 tccacgcctg acactcagtt ccgggtaggc agttcgctcc aagctggact gtatgcacga
     2881 accccccgtt cagtccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     2941 ggaaagacat gcaaaagcac cactggcagc agccactggt aattgattta gaggagttag
     3001 tcttgaagtc atgcgccggt taaggctaaa ctgaaaggac aagttttggt gactgcgctc
     3061 ctccaagcca gttacctcgg ttcaaagagt tggtagctca gagaaccttc gaaaaaccgc
     3121 cctgcaaggc ggttttttcg ttttcagagc aagagattac gcgcagacca aaacgatctc
     3181 aagaagatca tcttattaat cagataaaat atttctaggc tcatgagccc gaagtggcga
     3241 gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg
     3301 ccggtgatgc cggccacgat gcgtccggcg tagaggatct gctcatgttt gacagcttat
     3361 catcgatgca taatgtgcct gtcaaatgga cgaagcaggg attctgcaaa ccctatgcta
     3421 ctccgtcaag ccgtcaattg tctgattcgt taccaattat gacaacttga cggctacatc
     3481 attcactttt tcttcacaac cggcacggaa ctcgctcggg ctggccccgg tgcatttttt
     3541 aaatacccgc gagaaataga gttgatcgtc aaaaccaaca ttgcgaccga cggtggcgat
     3601 aggcatccgg gtggtgctca aaagcagctt cgcctggctg atacgttggt cctcgcgcca
     3661 gcttaagacg ctaatcccta actgctggcg gaaaagatgt gacagacgcg acggcgacaa
     3721 gcaaacatgc tgtgcgacgc tggcgatatc aaaattgctg tctgccaggt gatcgctgat
     3781 gtactgacaa gcctcgcgta cccgattatc catcggtgga tggagcgact cgttaatcgc
     3841 ttccatgcgc cgcagtaaca attgctcaag cagatttatc gccagcagct ccgaatagcg
     3901 cccttcccct tgcccggcgt taatgatttg cccaaacagg tcgctgaaat gcggctggtg
     3961 cgcttcatcc gggcgaaaga accccgtatt ggcaaatatt gacggccagt taagccattc
     4021 atgccagtag gcgcgcggac gaaagtaaac ccactggtga taccattcgc gagcctccgg
     4081 atgacgaccg tagtgatgaa tctctcctgg cgggaacagc aaaatatcac ccggtcggca
     4141 aacaaattct cgtccctgat ttttcaccac cccctgaccg cgaatggtga gattgagaat
     4201 ataacctttc attcccagcg gtcggtcgat aaaaaaatcg agataaccgt tggcctcaat
     4261 cggcgttaaa cccgccacca gatgggcatt aaacgagtat cccggcagca ggggatcatt
     4321 ttgcgcttca gccatacttt tcatactccc gccattcaga gaagaaacca attgtccata
     4381 ttgcatcaga cattgccgtc actgcgtctt ttactggctc ttctcgctaa ccaaaccggt
     4441 aaccccgctt attaaaagca ttctgtaaca aagcgggacc aaagccatga caaaaacgcg
     4501 taacaaaagt gtctataatc acggcagaaa agtccacatt gattatttgc acggcgtcac
     4561 actttgctat gccatagcat ttttatccat aagattagcg gatcctacct gacgcttttt
     4621 atcgcaactc tctactgttt ctccataccc gtttttttgg gctagcgaat tcgagctcgg
     4681 tacctttgag gtggttatga aaaaaattgc atgtctttca gcactggccg cagttctggc
     4741 tttcaccgca ggtacttccg tagctggcca gtctggccag ggagggcagt ctgggcagtc
     4801 tggtgactac aacaaaaacc agtactacgg catcactgct ggtccggctt accgcattaa
     4861 cgactgggca agcatctacg gtgtagtggg tgtgggttat ggtaaattcc agaccactga
     4921 atacccgacc tacaaacacg acaccagcga ctacggtttc tcctacggtg cgggtctgca
     4981 gttcaacccg atggaaaacg ttgctctgga cttctcttac gagcagagcc gtattcgtag
     5041 cgttgacgta ggcacctgga ttttgtctgt tggttaccgc ttcgggagta aatcgcgtcg
     5101 cgcgacttct actgtaactg gcggttacgc acagagcgac gctcagggcc aaatgaacaa
     5161 aatgggcggt ttcaacctga aataccgcta tgaagaagac aacagcccgc tgggtgtgat
     5221 cggttctttc acttacaccg agaaaagccg tactgcaagc taataaggcc aaggtggcca
     5281 agcttggctg ttttggcgga tgagagaaga ttttcagcct gatacagatt aaatcagaac
     5341 gcagaagcgg tctgataaaa cagaatttgc ctggcggcag tagcgcggtg gtcccacctg
     5401 accccatgcc gaactcagaa gtgaaacgcc gtagcgccga tggtagtgtg gggtctcccc
     5461 atgcgagagt agggaactgc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg
     5521 gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc tgagtaggac aaatccgccg
     5581 ggagcggatt tgaacgttgc gaagcaacgg cccggagggt ggcgggcagg acgcccgcca
     5641 taaactgcca ggcatcaaat taagcagaag gccatcctga cggatggcct ttttgcgttt
     5701 ctacaaactc ttttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     5761 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     5821 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     5881 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gca
//
